Metabolic Pathways |® 2. ot Hemoglobin Oxygen Dissociation Curve Hemoglobin: ees MELE Th 2 Pi RBC Preservation Anticoagulant Preservative Solutic RBC Prese vy a chiettcemermemms Anticoagulant Preservative, . = SS SOUS ween a Correlated with the sca! = ioieRot ste cca = whieh “useeldted See = is tie teal es Taenass. Sara RBC Preservation Anticoagulant (Prasedvadie’ in all ceo preservatives SQWkUMeS depleted of 2,3-0PG by the second week of storage - Stored RBCIs do regain the ability to synthesize 2,3-DPG after transfusion +24 hours: t© restore normal levels of 2,3-DPG + 6 hours. to reach normal levels RBC Preservation Anticoagulant Preservative Solu tia.nsim ie she iad ity of wanstused see Bebe PAS AMA a ERE BE Ne EB Dattng’ erage, a MaLUcitpe atethainerady-onthalate (oerh) ne CEMENT bane teams af girs Sie 'toe's ‘dening Sovage: a Rasiese cost ml Intex toe plastic RBC Preservation Anticoagulant Preservative RBC Preservation Additive Solutions Benefits of RBC Additive Solutions 1. Extends the Ghelf life of RBCs to 42 days by adding nutrients; BLAllows for the harvasting ot mail plasm a and platelets from the unit; Biproduces a ABC conceals! of low or viscosity that easier to infuse; ‘RBC Preservation Additive Se, tions preserving that are added to the RBCs after removal of the plasma with or without platelets. It contained in a satellite bag and is added to them les» — amine ne? iia BGWHOAS. three momen ee additive solutions mr ow vw contains ——w ae Saline, Adenine sens = and Glucose | == ae = AS-1 and AS-5 n= 4 Protects) against rwanesnteanmeententomes forage ftiatea == hem olvsis aca mae =RBC Preservation RBC Freezing - It is primavily used for autologous unit and the storage of rare blood types It involves addition of a cyroprotective agent to RBCs that are less than 6 days old Glycerol is used most commonly and is added to the RBCs slowly with vigorous shaking, thereby enabling the glycerol to permeate thomROrl 5. — RBC Preservat RBC Freezing RBC Preservation RBC , REMI E Less by which ATP and 2,3- DPG levels are restored or enhanced by metabolic alterations It is accomplished by incubating the RBC unit with 50mL of the rejuvenating solution for 1 hour at 378C - Then washed to remove the. rejuvenation solution and transfused Within 24 how nemenn ions ‘a