| | Name: I Demonstrator: Flow Diagram 1. Preparation of Sample ‘Orange Juice (40-50 ml) Beaker | Add 23tsp celte Stir to make slurry Vacuum fier Fitrate (used in Part 3) 3, Titration of range Juice 5 mL 2% KI olution, 2mLS% acetic acid, 5 drops starch ind Conical flask ‘Add 20-30 mi distiled water | te with NBS in burette ‘Lo. minutes Experiment 1: Vitamin C Content of Orange Juice 5 mL aliquot of filtered Experiment Day and Group: 2. Standardisation of Oxidisi ‘Accurately weigh~ 40 mg ascorbic acid Volumetric flask ~ make to 100 mL t 10 mt aliquot SmL2%KI solution 2mL 5% acetic acid 5 drops starch indicator Conical flask ‘Add 20-30 ml cistiled water Titrate with NBS in burette Repeat until tre agrees to ‘orange juice from Part 1 = within 0.2 me OF ny Pelt Repeat titration until titre agrees towithin 0.2 mL of| all ‘ Experiment 1 Pre-Lab Questions 1. Write the redox equation forthe reaction between ascorbic acid and N-bromosuccinimide. Please use structural formul ° ant oe vo ae ( oN dada ¢ sf ere : ey mal Ni ; at a. Ascorbic acid 2D “(6H9,) FHL Oo: C4 3(s)4 bCDEO 6C+¥-1220 Ce4IV Dehyaro ascorbic aid Cog Oy o 604 64) +6(--0 6c +b-n co 6c 46-0 C#+loe @VID \Which carbon stoma in Sic acid are oud gong to dehyro aso cd? eahonal gong). aa isk Unie Ale eeend cectan 3, What event marks the end point ofthe titration of ascorbic acid with N-bromosuccinimide? Briefly, why does this occur? LED gre chai tn salt fe tippetia libre 4. Chemwatch practice question: Use the Chemical Risk Assessment Sheet to find what precautions you need to take when handling N-bromosuccinimide. aos ‘Chemistry 2 Laboratory Manu\abertory Worksheets Experiment Results 2, Standardization of N-Bromosuccinimide (NBS) solution CcHaOg + CaHyBrNO —+ Cgllg0g + C\HsNO, + HBr ‘To standardise the NBS solution, first work out how much NAS is required to oxidize a known mass of pure ascorbic acid. The amount of ascorbic acid that was weighed out is known, and so the number of moles of NBS required to react completely with the amount of ascorbic acid ina 10 mi aliquot can be found (using the equation above). This allows you to work out the concentration of the NBS solution. Yo Mass of pure ascorbic acid: AS"... mg; Please note massisin miligram and not gram, Massis ‘made up to 100.0 ml in a volumetric ask. Use 10.00 mL aliquots. NB: You need to take into account ‘that you used 10.00 ml in your calculations, rather than the whole 100.0 ml. Sample number for titration Initial burette reading Final burette reading Volume of N8S son used ‘Average volume of NBS soln Concentration of NBS solution expressed asa) molarity; and b) mg ascorbic acid equivalent to 1,000 mL of NaS souton: a) Be. molt 1) .0:202, mgmt" (8: your ration resus ve the umber of mi of NBS required to react wth about 4.00 mg (se your acura mes of sori ai) (show cleulatons ere) | 1f2]3 8), 0.0401 /ay, 1 atest a 2 Rae poadaor [2774 asi 20e8 | 4 Rath onde * = 0-04 F mol /e = ee Ag (1£35 2 pied Ee 14.655, 0 by 40. NIFTY Cog aliquot) = 0 opi? mol/t Aes * 176,12 = 0.202 5/. sole (hemi 2 Laboratory Maal 50.20% mg fonExperiment 3.Thration of Orange Juice: ‘The exact concentration of NBS is knawn from Part 2, so you can now work out how much vitamin C (sscorbc acic) fs present in the aliquot of orange juice used. This allows you to work out the Concentration of vitamin Cin 100 mi of juice and hence you can calculate the mass of vitamin Cin 200 mLofjuce. entity of orange juice used:... golden, Cale + 5.00 mL aliquots used. Sample number for titration a ae a 1388 26730 436 36.45 22.5 Bk 22.5 I burette reading Faint, lure of NBS soln used (ml) 1$:8.. ge volume of NBS soln 22 35m 45.158 centration of Ascorbic Acid in juic ‘mg ascorbic acid / 100 mL Orange Juice (NB: You 5.00 mL aliquots of orange juice, 1g = 1000 mg ‘mass of ascorbic acid = 176.124 g mol? alataostere) ne cv = 0.04? * 6.02235 n(wBo) = 2.5445 00°" a Y nid) as ) . g08tt2? Ans (sat) (19 i) = paar 0.945157 = R0tAra fie ~ ASS Fy ide, 2 abort Manual ae| sal Experiment 1 Post-Lab Questions 1. (Hearning question) Some fruits are better sources of vitamin C than the others. For ‘example, oranges have 53 mg of vitamin C/100 g of fruit while apples have only 6 mg. Describe how you can modify the experiment above in order to measure the amounts of \tamin C present/100g in ruts e.g lemonylime or kiwifruit -E.omh. Octet ntl ialtt Sam. ltd... a shen Habra tol prac. 319), 0b. te dase, te sepeat Sint tite Surie Alta rileess.A6rei. all fect fe. eat7th;. Raped eadleh. lhe. 81K. in. geil... uinble cB, 2. The recommended dietary intake for vitamin C is 60 mg per day. According to your ‘experimental resuits, what volume of orange juice should you consume to obtain the ecommended dose? Show your calculations Tek eR ct MSAD Ping. til: ©, nat a The dif betetee 60. salt 45.187 Ma $8309... IMS (98.182. 20.3297 4A BAKE H), XIere ee ae Jn A tbe erated RO elt .$4.0htsin, bo 9 ty : 4 = vs 3. Give areason 2s to why your calculated amount of. diferent to that stated on the container. Lit... Antec isn. avi Fase sss skaple eamipond: aloe RA tae) yl ttl tod mened bef 6 8 PALE Atif tHE $i it... ttbalhccas tee, eaneved fetes. alla. lela, pnts BEE. ldo 6. ascorbic ald (determined in Part 3) is (mrisey 2 aberstory Manual sotpein 1 aboratony Worksheets Marking Scheme A eA ae Pretab Questions R Question B auestion2 R auesion3 Questions c TOTAL 2 Resule = art2: Volumes for NB standardisation titration concordant tires) R 'NBS concentration calculations uy Part 3: Volumes for 04 titration R Vitamin concentration calculations 's Tora ha Post ab Questions Question R Question 2 A Question 3 R ToTAL b Teocker Check a Formatting a Significant Figures R TOTAL 26 Crerisey 2 Laboratory Maral 6ot6