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For each of the following biochemical tests complete the following:

1. Provide a brief description of what the test does, making sure to include whether or not extra
reagents need to be added after incubation to see results.
2. Discuss how you would determine if a test was positive or negative- then discuss why the test
is positive or negative.
3. Provide examples of “scoring” if it exists with the test. (Litmus milk, Triple sugar iron agar)
Along with the interpretations of the scoring.
4. You will need to search for much of this information online. If you have questions, please ask!

Litmus Milk

Urea Broth

Nitrate Broth

MRVP

Dextrose Broth

Sucrose Broth

Lactose Broth

Triple Sugar Iron Agar

Citrate Agar

Skim Milk Agar

Synder Agar

SIM Agar

Starch Agar

Potato Dextrose Agar

Mannitol Salt Agar

Eosin Methylene Blue Agar

MacConkey Agar

Mueller Hinton Agar

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