18BTC201J- GENE MANIPULATION AND GENOMICS LABORATORY B.Tech (Biotechnology) TI YEAR/ V SEMESTER Name Register no DEPARTMENT OF BIOTECHNOLOGY SCHOOL OF BIOENGINEERING FACULTY OF ENGINEERING AND TECHNOLOGY SRM INSTITUTE OF SCIENCE AND TECHNOLOGY KATTANKULATHUR- 603 203 20 -20RM BONAFIDE CERTIFICATE Register no: Certified to be the bonafide record of work done by. of B.Tech Biotechnology Degree course in the 18BTC201J- Gene Manipulation and Genomics Laboratory at SRM Institute of Science and Technology, Kattankulathur, during the academic year 20 - 20 Lab Incharge Date: Head of Department Submitted for University Examination held in _ at SRM Institute of Science and Technology, Kattankulathur. Date: Examiner-1 Examiner-2Name of the student: Register no INDEX Date Name of the experiment Page No Date of submi Remarks Preparation of DNA insert — ion enzyme digestion of Genomic Preparation of DNA inserts - (II) Elution of. DNA Hind III digest (DNA inserts) from agarose Gel Preparation of plasmid Vector — Digestion ‘of pUCI8 by Hind III restriction enzyme Preparation of rDNA for cloning - Ligation of plasmid vector with lambda DNA insert [Bacterial competent cell preparation for transformation Transformation of (DNA in to competent cells (DHSa strain) !analyzing Transformants by Colony PCR [Total RNA Isolation DNA synthesis jantitative Real-Time PCR k