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The American Journal of Sports

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Arthroscopic Meniscus Allograft Transplantation in Male Professional Soccer Players: A 36-Month


Follow-up Study
Maurilio Marcacci, Giulio Maria Marcheggiani Muccioli, Alberto Grassi, Margherita Ricci, Kyriakos Tsapralis, Gianni
Nanni, Tommaso Bonanzinga and Stefano Zaffagnini
Am J Sports Med published online November 8, 2013
DOI: 10.1177/0363546513508763

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Arthroscopic Meniscus Allograft


Transplantation in Male Professional
Soccer Players
A 36-Month Follow-up Study
Maurilio Marcacci,* MD, Giulio Maria Marcheggiani Muccioli,*y MD, Alberto Grassi,* MD,
Margherita Ricci,z MD, Kyriakos Tsapralis,z MD, Gianni Nanni,z MD,
Tommaso Bonanzinga,* MD, and Stefano Zaffagnini,* MD
Investigation performed at Istituto Ortopedico Rizzoli, Università di Bologna, Bologna, Italy

Background: Meniscus allograft transplantation (MAT) is an option for symptomatic patients who have undergone subtotal
meniscectomy and can potentially result in pain relief and increased function.
Hypothesis: Professional soccer players would benefit from arthroscopic MAT in terms of pain, knee function, and return to play
at 36-month follow-up.
Study Design: Case series; Level of evidence, 4.
Methods: Twelve male patients who had undergone MAT (6 medial, 6 lateral) were prospectively evaluated at 12- and 36-month
follow-up. The mean age at the time of surgery was 24.5 6 3.6 years (range, 19-29 years), and the mean time from meniscectomy
to surgery was 37 6 31 months (range, 2-82 months). The transplantation was performed in patients who had undergone subtotal
meniscectomy using an arthroscopic bone plug–free technique with a single tibial tunnel plus ‘‘all-inside’’ meniscus sutures. The
anterior horn of the transplanted meniscus was sutured to the capsule and to the remnant of the anterior horn of the native menis-
cus. Seven patients (58%) underwent concurrent procedures. All patients were evaluated at follow-up by the Tegner, Lysholm,
International Knee Documentation Committee (IKDC) subjective, IKDC objective, Western Ontario and McMaster Universities
Osteoarthritis Index (WOMAC), and visual analog scale (VAS) for pain scores. Information regarding rehabilitation, return to
play, and return to official competition was recorded.
Results: Eleven of the 12 patients (92%) returned to play soccer. At 36-month follow-up, 9 patients (75%) were still playing as
professionals (Tegner score of 10), whereas 2 patients (17%) were playing as semiprofessionals (Tegner score of 9). The mean
time from surgery to the end of rehabilitation was 7.5 6 2 months, whereas the mean time to official competition was 10.5 6
2.6 months. Patients demonstrated significant improvements on the median Tegner score from 8 (interquartile range, 3-10) to
10 (interquartile range, 9-10) (P = .0391); the mean Lysholm score from 67 6 14 to 92 6 10 (P = .0021); the mean IKDC subjective
score from 61.8 6 16.3 to 85.3 6 9.8 (P = .0026); the mean IKDC objective score from 1 A, 8 B, 1 C, and 2 D to 7 A and 5 B (P =
.0077); the mean WOMAC score from 77.1 6 15.9 to 92.2 6 9.1 (P = .0242); and the mean VAS score from 61 6 16 to 29 6 32 (P =
.0029) at 12-month follow-up. There were no significant improvements in these outcomes at 36-month follow-up. One patient
developed a knee infection after MAT plus anterior cruciate ligament allograft reconstruction. This complication was successfully
treated, but the patient stopped playing soccer (Tegner score of 3 at 36-month follow-up), and this was considered a failure (8%).
Conclusion: Arthroscopic MAT in professional soccer players allowed a return to play at the same level (Tegner score of 10) in
75% of the cases at 36-month follow-up.
Keywords: knee; arthroscopic surgery; meniscus allograft transplantation; professional soccer players

To treat meniscus tears in professional soccer players (a because of the long rehabilitation period required after
particular population of patients in whom these injuries meniscus sutures3 and the high failure rate (20.2%-
are very common2), meniscectomy is sometimes preferred 24.3%) reported after this surgery.19
to meniscus suturing by patients and surgeons. This is After removal of the meniscus, recurrent joint effusions
and pain may develop, affecting the career of athletes.10,15
It is also well accepted that complete or even partial menis-
cectomy can lead to osteoarthritis in the affected compart-
The American Journal of Sports Medicine, Vol. XX, No. X
DOI: 10.1177/0363546513508763 ment in the long term8,9 and in rare cases to severe acute
Ó 2013 The Author(s) chondrolysis, especially in soccer players.18

1
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2 Marcacci et al The American Journal of Sports Medicine

Meniscus allograft transplantation (MAT) is a viable and standing anteroposterior and Rosenberg radiographs
option for symptomatic patients who have undergone before surgery to determine their suitability for MAT. A
total/subtotal meniscectomy and can potentially result in detailed medical history was obtained, and a complete phys-
pain relief and increased function.20,23,24,27 However, there ical examination was also performed.
is no consensus on the use of MAT in a high-demand pop- Fresh-frozen (–80°C) nonirradiated and non–antigen-
ulation such as soccer players. Furthermore, opinions vary matched allografts were used. The age criterion for donors
on the amount of arthritic changes that would be contrain- was 15 to 35 years. Radiographic measurements and
dications to meniscus transplantation.5 anthropometric parameters were used to establish the cor-
This procedure has been recently performed in nonpro- rect size of the graft.
fessional athletes, and the results have been promising.1,6 All athletes underwent MAT in our Sports Medicine
What is still unknown is whether this procedure can be an Department, which was performed by 2 senior authors
effective treatment for ‘‘postmeniscectomy syndrome’’ in (M.M. and S.Z.). The transplantation was performed using
professional soccer players. an arthroscopic bone plug–free technique. A single tibial
The purpose of this study was to report clinical out- tunnel was used to fix the posterior horn, and the graft
comes and the return to sport in a consecutive series of was fixed to the capsule with ‘‘all-inside’’ stitches (nonab-
symptomatic male professional soccer players who had sorbable ULTRABRAID No. 0 and poly-L-lactide bioab-
undergone previous subtotal meniscectomy and were trea- sorbable ULTRA FAST-FIX, Smith & Nephew, Andover,
ted with arthroscopic MAT. We hypothesized that the use Massachusetts). The anterior horn of the transplanted
of this technique would result in reduced symptoms meniscus was then fixed to the remnant of the anterior
(pain), lead to improved knee function, and allow a return horn of the native meniscus by an outside-in standard
to play at the same premeniscectomy level at a minimum suturing technique. The anterior horn of the transplanted
36-month follow-up. meniscus was also fixed to the capsule with the sutures
that were previously placed on the anterior horn (during
graft preparation) tied through the corresponding arthro-
MATERIALS AND METHODS scopic portal. Patients with ligamentous instability under-
went concurrent ligament reconstruction surgery to
All professional soccer players who underwent arthro-
restore stability. One patient with focal Outerbridge21
scopic MAT without bone plugs at our institution between
grade IV cartilage disease in the affected joint underwent
2008 and 2010 (a part of a larger prospective database of
concurrent osteochondral scaffold implantation. Another
MATs) were included in this study. More specifically,
patient who had previously undergone arthroscopic autol-
inclusion criteria were identified as (1) unicompartmental
ogous chondrocyte implantation (ACI) presented with an
knee pain after subtotal meniscectomy (meniscus loss
Outerbridge21 grade II cartilage lesion at the time of MAT.
.75%), (2) a stable knee or anterior cruciate ligament
The postoperative rehabilitation protocol started with
(ACL) deficiencies stabilized at the time of the index sur-
a 2-week period with no weightbearing and immobiliza-
gery, (3) a patient between 15 and 55 years of age (on the
tion, followed by toe-touch weightbearing, restriction of
basis of previous studies7), and (4) a contralateral healthy
range of motion (ROM) (0°-90° during weeks 3-4 and
knee. Exclusion criteria included (1) concomitant posterior
then free ROM), isometric exercises, and closed chain
cruciate ligament insufficiency of the involved knee, (2) dif-
strengthening. At week 6 postoperatively, full weightbear-
fuse Outerbridge21 grade IV disease in the affected joint,
ing was started, and patients were allowed to fully flex the
(3) uncorrected malformations or axial malalignment in
knee. Sport-specific exercises started after 3 months, and
the lower extremity, (4) systemic or local infections, (5) his-
a return to noncontact activities was not allowed until
tory of anaphylactoid reactions, (6) systemic administra-
the fourth month. Patients were advised not to resume
tion of any type of corticosteroid or immunosuppressive
playing soccer before 8 months postoperatively.
agent within 30 days of surgery, (7) evidence of osteonecro-
The sizing protocol,22,26 operative technique,17 and
sis in the involved knee, (8) history of autoimmune dis-
rehabilitation protocol12,17 were followed as described in
eases, and (9) neurological abnormalities or conditions
the literature.
that would preclude the patient’s adherence to the rehabil-
itation program.
Patient Data
Planning, Graft Selection, Sizing,
Surgical Technique, and Rehabilitation Thirteen patients met the inclusion criteria of the study.
One patient was excluded, as he had not been playing soc-
All patients were screened with magnetic resonance imaging cer for more than 1 year because of problems not related to
scans, standard (anteroposterior and lateral) radiographs, his knee status. All of the remaining 12 patients were male

y
Address correspondence to Giulio Maria Marcheggiani Muccioli, MD, Laboratorio di Biomeccanica ed Innovazione Tecnologica, Istituto Ortopedico
Rizzoli, via di Barbiano, 1/10, 40136 Bologna, Italy (e-mail: marcheggianimuccioli@me.com).
*Clinica Ortopedica e Traumatologica II, Laboratorio di Biomeccanica ed Innovazione Tecnologica, Istituto Ortopedico Rizzoli, Università di Bologna,
Bologna, Italy.
z
Isokinetic FIFA Medical Centre of Excellence, Bologna, Italy.
The authors declared that they have no conflicts of interest in the authorship and publication of this contribution.

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Vol. XX, No. X, XXXX MAT in Professional Soccer Players 3

TABLE 1
Patient Dataa
BMI at Time
Age at BMI at Final Chondral From
Patient Age at Final Surgery, Follow-up, Damage at Meniscectomy,
No. Surgery, y Follow-up, y Knee Meniscus kg/m2 kg/m2 Previous Surgery Concomitant Surgery Surgery mo

1 23 26 Right Medial 22.8 22.8 ACL, MM, LM R-ACL III, MTP 42.8
2 25 28 Left Medial 23.8 23.5 ACL, MM, ACI Microfracture (MFC) III, MFC 78.7
3 19 22 Left Lateral 23.4 23.4 ACL, LM — — 1.8
4 23 26 Right Lateral 22.7 22.7 LM Microfracture (LFC) III, LFC 56.0
5 24 27 Left Medial 23.5 23.5 ACL, R-ACL, medial — — 6.5
meniscus suturing, MM
6 29 32 Left Lateral 22.2 22.2 LM, microfracture (LFC) OCS (LFC) IV, LFC 29.3
7 20 23 Right Medial 23.4 23.4 ACL, MM R-ACL — 6.0
8 26 29 Right Lateral 22.5 22.8 2 LM Microfracture (LFC) III, LCF 12.8
9 26 29 Right Medial 25.1 25.1 MM Chondrocyte III, MFC 35.2
harvesting
10 27 30 Left Lateral 23.8 24.0 2 LM — II, LFC 70.5
11 21 24 Left Lateral 24.2 23.5 LM — II, LFC and LTP 19.4
12 31 34 Left Medial 20.8 20.8 ACL, ACI, loose — II, MFC 85.9
body removal

a
ACI, autologous chondrocyte implantation; ACL, anterior cruciate ligament; BMI, body mass index; LFC, lateral femoral condyle; LM, lateral meniscectomy;
LTP, lateral tibial plateau; MFC, medial femoral condyle; MM, medial meniscectomy; MTP, medial tibial plateau; OCS, osteochondral scaffolding; R-ACL, ante-
rior cruciate ligament revision with allograft.

professional soccer players and were included in this study. different evaluation times and also before injury retrospec-
Five patients (42%) were still playing soccer at the time of tively on the basis of patient recall).
enrollment, but with performance impairment due to knee Data regarding rehabilitation and return to sport were
pain, while 7 patients (58%) played soccer as professionals collected as well. Dates of admission and discharge from
in the last year, but they were unable to play at the time of the rehabilitation clinic were recorded to establish the
enrollment. Their mean age at the time of surgery was duration of the rehabilitative program. The number of
24.5 6 3.6 years (range, 19-29 years). For MAT, the medial rehabilitative sessions performed at the gymnasium, in
meniscus was involved in 6 patients (50%) and the lateral the pool, and on the field was recorded. The time to return
meniscus in the remaining 6 patients (50%). The mean time to full training with the team was defined as the interval
from meniscectomy to surgery was 37 6 31 months (range, between surgery and discharge from rehabilitation, while
2-82 months). The meniscus lesions of all patients were the time to return to play was defined as the interval
related to sport trauma. Seven patients (58%) underwent 1 between surgery and the first official match.
concurrent procedure with MAT (2 revision ACL reconstruc-
tions with allografts, 3 microfractures, 1 osteochondral scaf- Statistical Analysis
folding, 1 chondrocyte harvesting). See Table 1 for details.
Statistical analysis was performed using Analyse-it 2.00
Follow-up Evaluation (Analyse-it Software Ltd, Leeds, United Kingdom). Statis-
tical comparison between the preoperative and follow-up
The primary outcome (returning to play at the same level) parametric scores was performed using the Student t
and time points were defined before enrollment. The play- test. The population was tested for normal distribution
ers were prospectively evaluated before surgery and at 12- before the t test was applied. For differences between
and 36-month follow-up by 2 independent orthopaedic sur- time points in the Tegner score, the nonparametric Wil-
geons, who did not take part in the surgery and were coxon test was used. For differences in the objective
blinded to preoperative patient status, affected side IKDC score, the Pearson x2 test was utilized. Comparison
(medial or lateral meniscus), and concurrent procedures. between groups based on medial versus lateral meniscus,
Every complication related to surgery and any reinterven- isolated versus combined MAT, and mild versus severe
tion(s) were recorded during the follow-up period. chondral damage was performed using the Student t test.
A 100-point visual analog scale (VAS) was used to A life table survival analysis with the log-rank test was
assess the degree of knee pain. Functional follow-up used to compare cumulative rates of return to training
included the subjective International Knee Documentation and competition in the subgroups.
Committee (IKDC),14 Lysholm,25 and Western Ontario and The level of significance was set at P \ .05. Results are
McMaster Universities Osteoarthritis Index (WOMAC)4 expressed as the mean 6 standard deviation for paramet-
scores as well as the objective IKDC score.13 The overall ric values and the median (interquartile range [IQR]) for
Tegner activity level25 was also recorded (obtained at the nonparametric values.

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4 Marcacci et al The American Journal of Sports Medicine

TABLE 2
Clinical Outcomesa

Tegner Lysholm Subjective IKDC Objective IKDC WOMAC VAS


Return to Return to
Patient No. Preinj Preop 1 y 3 y Preop 1 y 3 y Preop 1y 3y Preop 1y 3 y Preop 1y 3y Preop 1 y 3 y Training, mo Match, mo

1 10 8 10 10 75 100 100 56.3 82.8 98.0 D B A 97.9 100.0 100.0 40 10 0 8.0 12.0
2 10 10 10 10 71 99 99 71.3 93.1 93.1 A A A 89.6 96.9 96.9 70 10 10 4.4 8.0
3 10 1 10 10 26 99 99 16.1 92.0 98.0 C A A 36.5 96.9 96.9 70 0 10 6.2 6.7
4 10 10 9 9 81 81 95 70.1 62.1 93.1 B B B 86.7 86.7 91.0 70 90 15 6.7 12.1
5 10 6 9 9 69 99 99 56.0 93.0 98.0 B A A 68.0 96.9 98.0 60 10 10 9.0 12.0
6 10 3 7 10 71 81 99 56.3 82.0 93.1 B B A 68.0 93.0 96.9 60 35 10 12.0 15.7
7 10 3 2 3 50 68 65 56.0 71.2 65.0 D B B 68.0 70.0 75.0 80 90 60 6.2 —
8 10 8 10 10 75 95 81 71.3 92.0 82.8 B A B 86.0 93.0 91.0 70 15 20 6.2 7.5
9 10 10 10 10 71 95 95 71.3 85.0 93.1 B B A 82.0 93.0 96.9 45 20 10 8.5 10.6
10 10 3 10 10 69 99 99 71.3 92.0 96.0 B A A 86.0 96.0 98.0 55 15 10 5.8 8.8
11 10 10 10 10 71 95 99 76.0 93.0 98.0 B A A 82.0 96.9 100.0 30 0 5 7.0 8.0
12 10 10 10 10 69 95 95 70.0 85.0 82.0 B B B 75.0 86.7 91.0 80 50 25 8.0 12.0

a
IKDC, International Knee Documentation Committee; preinj, preinjury; preop, preoperatively; VAS, visual analog scale; WOMAC, Western Ontario and
McMaster Universities Osteoarthritis Index.

Ethics
Study approval was obtained from the institutional review
board. Informed consent forms complied with European Union
laws and were signed by the patients before enrollment.

RESULTS

All 12 patients were available for 12- and 36-month follow-


up. Eleven of the 12 patients (92%) returned to play soccer.
At 12-month follow-up, 8 (67%) of the patients returned to
playing soccer at the same activity level (Tegner score of
10) as before injury. The percentage increased at 36-month
follow-up, with 9 patients (75%) playing as professionals
(Tegner score of 10). Two patients (17%) were playing as
semiprofessionals for reasons not directly related to knee
performance (Tegner score of 9).
At 12-month follow-up, all scores improved significantly
from baseline. The median Tegner score improved from 8
(IQR, 3-10) to 10 (IQR, 9-10) (P = .0391); the mean Lysholm
score from 67 6 14 to 92 6 10 (P = .0021); the mean subjec-
tive IKDC score from 61.8 6 16.3 to 85.3 6 9.8 (P = .0026); Figure 1. Trends in return to training (straight line) and return
the mean WOMAC score from 77.1 6 15.9 to 92.2 6 9.1 (P = to official match play (dotted line) for all evaluated patients.
.0242); the mean VAS score from 61 6 16 to 29 6 32 (P =
.0029); and the objective IKDC score from 1 A, 8 B, 1 C,
and 2 D to 7 A and 5 B (P = .0077). At 36-month follow- to starting to train was 7.3 6 1.9 months, while the mean
up, the clinical evaluation showed similar results compared time to return to official competition was 10.3 6 2.7
with those of the 12-month follow-up evaluation. The months (Figure 1).
median Tegner score was 10 (IQR, 10-10), the mean No significant differences in the return to training and
Lysholm score was 94 6 10, the mean subjective IKDC score return to first official competition were found between
was 90.9 6 9.8, the mean WOMAC score was 94.3 6 6.9, medial or lateral MAT, patients with none/mild
and the mean VAS score was 15 6 15. Furthermore, 8 knees (\Outerbridge21 grade III) or severe (Outerbridge21
were classified as A and 4 as B according to the objective grade III) chondral damage, and isolated or combined
IKDC score. See Table 2 for detailed outcomes and pain MAT (Figure 2).
scores of each patient. During the follow-up period, 3 patients (25%) under-
The mean duration of rehabilitation was 6.9 6 1.8 went 1 surgical procedure because of issues not directly
months, with a mean number of 154 6 62 sessions per- related to MAT. One patient (8.3%) underwent arthro-
formed at the gymnasium (95 6 39), in the pool (35 6 scopic plica excision at 3 months after MAT, 1 (8.3%)
13), and on the field (24 6 16). The mean time from surgery underwent ACI at 1 month after the main surgery, and

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Vol. XX, No. X, XXXX MAT in Professional Soccer Players 5

Figure 2. Differences between return to training and return to official match play in different subgroups of patients depending on
(A) chondral damage, (B) involved meniscus, and (C) concomitant procedures. No significant differences were reported (P \ .05).

another patient (8.3%) underwent arthroscopic intra- DISCUSSION


articular loose body removal at 8 months after MAT. At
second-look arthroscopic surgery, the grafts presented no The most important finding of the present study was that,
tears and showed good healing to the capsule. at 36-month follow-up, 92% of the players were able to
One patient developed a knee infection after combined return to play soccer, and 75% were able to return to their
medial MAT plus revision ACL reconstruction with an allo- preinjury professional level of activity (Tegner score of 10)
graft. This player was successfully treated with oral antibi- after arthroscopic MAT without bone plugs. Our patients
otics and 2 arthroscopic lavages. Although he completed reported statistically significant improvements in func-
the rehabilitative program, he was not able to return to tional outcomes (Tegner, Lysholm, IKDC, WOMAC) and
playing soccer at any level because of knee pain. He had symptoms (VAS for pain) at 12 months after surgery, and
a Tegner score of 3 at 36-month follow-up, and this was these results remained stable at 36-month follow-up. These
considered a failure (8%). results are comparable with those of a series of

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6 Marcacci et al The American Journal of Sports Medicine

nonprofessional soccer players or athletes. Alentorn-Geli follow-up. Longer term follow-up is necessary to provide
et al1 reported that 85.7% of players returned to play com- information on the efficacy and longevity of MAT in this
petitive soccer at lower levels (Tegner score of 9) at a mean particular population of athletes.
follow-up of 36 months. Chalmers et al6 reported that 77% The present work has several limitations. The main lim-
of high-level athletes (mostly baseball and basketball play- itation is the small sample size and the broad range of con-
ers) returned to their previous level of activity (Tegner comitant knee injuries within our cohort. Unfortunately, it
score of 8) and that 70% were able to return to their desired is inevitable that this complex patient population has mul-
level of play at a mean follow-up of 3.3 years. The higher tiple knee comorbidities that require intervention at the
rate of return to competitive play reported by Alentorn- time of MAT.1,6 The same limitation applies to most of
Geli et al1 could be explained by the fact that in their the recent literature regarding complex knee reconstruc-
study, no ipsilateral knee ligament reconstruction or carti- tions.11,16,20 Another limitation is the relatively short-
lage repair surgeries were performed before or at the time term follow-up, which raises the question of whether deg-
of MAT. In fact, Chalmers et al6 reported a rate of 54% of radation of outcomes will be seen with time, as other series
concomitant procedures (mostly cartilage repairs), similar have shown. Moreover, the lack of follow-up with advanced
to the 58% reported in the present work. imaging or second-look arthroscopic surgery does not allow
In our series of male professional soccer players, the us to comment on the status of the transplanted menisci in
mean time from surgery to the start of training was these cases.
7.3 6 1.9 months, and the mean time to return to official
competition was 10.3 6 2.7 months. Alentorn-Geli et al1
reported that patients returned to play soccer after
a mean postoperative period of 7.6 months, but they did CONCLUSION
not specify if it was for training or official competition.
Arthroscopic MAT in professional soccer players allowed
Chalmers et al6 recalled a mean time to return to the pre-
them to return to play at the same level (Tegner score of
injury athletic level of 16.5 6 6.5 months, but their
10) in 75% of the cases at 36-month follow-up.
patients were collegiate athletes (Tegner score of 8).
No significant differences in the return to training and
return to first official competition were found between
medial or lateral MAT, patients with none/mild or severe REFERENCES
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Vol. XX, No. X, XXXX MAT in Professional Soccer Players 7

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