bacteia Genehcs

Gene: Ba_ic unt o hhexitah C

Role Bactesia in Study ogeneics

Bactebia in most Specie` Contaihs a Sihgle
e ely 29 onth@n
chÝomasome, So
Can o Stud oehehcs as thedre
'S ho mes Kihg oheS due tb preseha o Single chtomo
Some

Backeata Shows
high dagsee h heaitan le aho vaàiation
which Cab ba
2a5iy Seen bobseaviha di? Staihs e
Bacte a
Bacteaia Can ps Odu Ce appk 3500 es o ptofens So
w Can ea6i obsebve wo& Kihg s d aniho
acidS and 2hm es 4us btudu îha Bachesia alohe
oa to
apd guowh o Baceria changes upto many gehed-
ons Cah oe
Seeh ah whch be u$ed to study 2unc-
AOnihg and inhabitah Ca oenesS
Bacteia

no-pc Changes Yhehorapic Changas

o Due b mutahoh
o e d Envidonment oX
Also alled Physiolgical genehcs
Chorges
 Chahgges
phehotybiC
Envsonmertal hysioloaiCal
to the
outSde

Bacteaial es changaS acc hange i)

there S no chat
Condi HonS, hough oue to his
Bacteag
the hohype/gehes o he
hthehc ih Sunlight
Eugleba (Photosy Suh ight
CPaasiHc in abseno o

Non-Hucoid ColohieS
Mucoid Colonies o Haasobac o Same bactesiunm
eiom Jadio bacteg
ih
Csesente o) SucTaSe Salt
medium

genopic Changas in Bactea

he ene.
nucleohde Sequehe o
Motathon: Changein he Shows mu fahoh
IS Caleo
A5orgahiSm ha
Hutaht
o mutant:
amjho
holeahda Kesults
ih Cha nag O
Any chanae n alteihg the Whole gene
ohen, thS
a pr
aCid ih
voos c8gahiS m
ione-
a s e Rand om
Kabe_aro spoh
mutahohS
Natogally
o
Na aRalutakhons oe one in a milon t
a one in 10 bilion Somo hmeS

Thusit6 colt o
gecoghiSe a mutant
Graup hon- HutahiS. aom q

Hat Del bruck and

Salv@dou Luia proved backeaja have
cLohe hegidiy eatuS 1ke *hat o buahS
highex oanis mS od othe

Some BactexiaS wese

hatugally gesistant to lact@io
bhaQs and heig descendahtS also
had he Same res
stan Ce hey psoed how hese
xeSolt oh Hutahong nar OCcdg ed
baChei aS were ha
hatugally
MotahonS ase
COmpletly
andom ghd Spohtahe oos waS
baoved by G Joshu and Esthex
spain ent Knon os ho Ledag bas g by
PapliCa plahirg expameht.
Palplica-plakna Erpaaiment:(used Joy isalohg nutdo
-o i g oE.Col).
S+ep-I Take a Bacterial Suspensioh and oxpose
TD a
mutaoenic ageht Ke 0.. gaus

Backoia
Colohjes
Bacteia 0 Sed (E.oli)
 DATE-
AGE

Steb T Sample Jsom the achia Suspehsion s

buh oh a agax and nutient gidh medrom' and Le2
o incubahon and he þositoh o he olohies
hoted

Step LA stenle Rophca blahha o a cheeSe cdoth is

Gontly poeSSed against the BacteQ olohie5 on.
heh it is te placed oh a Gnd
Simila petidish w
notient dolicent med um (Glu+ Jhobganic Salis)
Note T should be done veay _pseci Sely wihoot
dist uxbing he bosHoh o Co loni es
Step : he hutaient deident hadio m i6
n ubahOn lator
Ste : The hew 3acter gl Colohies a&obse ved
7noh-&9hd om
Teente: HotahohS Cge hos-SkorRbeeUS t Should
he taken Up by
Rve&y baceria ahd all bactetH
must Show Saue
Colonies dn he Same_ pla i
he aga dekident mediu
m but ih geal So
Colon ies dis appeated

Rosylt: Motahons age CompleHy Random

 A Repica ó Same bacBeiq Colonies s Geneated, Trat S
why it S Khowh a5 Roplic plahng expei meh}

Petoosg-toriahgjpothe sis2
Pcc tb Dalbong and Lwng: mutationS gealy ole behds
upon expo su to BacBebig, hen theere o sahe
Kind o bacteias wheh exposed to the phages mest
Show Same Kn humber o Sogvivihg Colonies hd i
mutahonS ahd sandom there Should b a lat2
dib ih huheber o, Colonies
Delburg Luia expeaimond Plso aled lockuahion test

Bacteia olture havig 10cells/n

Cultuse divdlaol into mahy

SingleColtuge patS
wth Simi CompaSItHon erbosedto phogs

exposed ophages
00A B C
 Tngosence
tophages h mahy Smo
otuseH itwa6 ,2ypased
ducdo Shat oh
Pooko Hons , Rus So tHnhdoa Slow
Show mutqhoh but qt vey
ColoneS

o Colohies wB vaihg
vhile in 8 ho humber muta hohS
the Cwl tuses
indicahhg în o
Som e

poped Op andomly

QCRaent Baceta)

CHukanhS)
6
Eaaly Hokah on CNo MutaHon)

e
Late Mutahoy

esult: Hutohons ase Completly Rando m

Psa ha a baho
8aae als hey Kahe
USme ab mutahon aayto wo ail yiS
USe ab di ocphions mai odada to Sae Sin
humba Colonies aai

8Bahot Saag e Cultuse they Kisi rmai mutoton aay

mai hahi a