ADVIA-2120i Hematology Training

ADVIA® 2120i Hematology System
Virtual
Training Workbook
Siemens Healthineers
ADVIA® 2120i Hematology System

Virtual Training Workbook
ADVIA® 2120i Hematology System T02006.005 Effective Date: 02/22/2021

© 2021 Siemens Healthineers Inc. All rights reserved.
This manual, and the software described in this manual, are copyrighted. No part of this may be
copied, reproduced, translated or reduced to any electronic medium or machine-readable form
without the prior written consent of Siemens Healthineers.
ADVIA, TESTpoint, OPTIpoint, SETpoint and TIMEPAC are trademarks of Siemens Healthcare
Diagnostics Inc.
All other trademarks are the property of their respective owners.
ii ADVIA® 2120i Hematology System

Table of Contents
1: Welcome
2: System Overview
3: Starting Each Shift
4: Quality Control I
5: Sample Processing
6: How the System Works
7: Methods
8: Quality Control II
9: Scheduled Maintenance
10: Gains
11: Calibration
12: Troubleshooting
ADVIA® 2120i Hematology System iii

iv ADVIA® 2120i Hematology System
Welcome
1 Welcome
Welcome to Training
Siemens Healthineers Training would like to welcome you to basic operator training
on the ADVIA 2120i Hematology System.
This course is designed to teach you the skills needed to operate and maintain the
ADVIA 2120i Hematology System. Our staff welcomes the opportunity to present
this virtual training program to you.
Training Material
This training workbook includes an agenda, learning goals, and practical exercises.
ADVIA® 2120i Hematology System 1-1

Welcome
Course Objectives
Upon completion of the class, you will be able to:
• Identify system hardware components and reagents
• Navigate through software menus
• Perform Starting each Shift
• Prepare and perform Quality Control processing
• Demonstrate how to import a new lot of QC
• Perform sample processing
• Describe how the system works
• Perform and record scheduled maintenance
• Identify the methods used on the system
• Review the gains adjustment process using the Gains Wizard
• Review the calibration process using the Calibration Wizard
• Utilize the Operator’s Guide to locate sample / system flags
1-2 ADVIA® 2120i Hematology System

Welcome
Agenda
Day 1
• System Overview
• Starting Each Shift
• Quality Control I
• Sample Processing
• How the System Works
• Methods
• Quality Control II
Day 2
• Scheduled Maintenance
• Gains
• Calibration
• Troubleshooting

Welcome
ADVIA® 2120i Hematology System Virtual Training

Course Validation Checklist
The participant places a checkmark beside the competency when it is completed.
When all competencies are checked, the Observer and Participant sign and date
below as record of completion.
Topics Competencies Completed

System Overview Identify system hardware components
Identify system reagents
Navigate the key areas of the software
Starting each Shift List the steps for Starting each Shift
List the steps to empty waste
Locate and check the overflow bottle
Recognize reagent inventory needs and replace if
needed
Evaluate background counts
Quality Control Prepare and perform Quality Control processing
Review and validate QC results
Demonstrate how to import a new lot of QC data
Interpret QC data
Demonstrate printing and exporting of QC reports
Sample Processing Perform sample processing in all modes
(Autosampler, MOTS, and MCTS)
Identify the sample statuses
Locate and validate sample results
Demonstrate printing of sample results
Use Order Entry to create workorders
Use Manual Sample ID option to enter samples
How the System Works Identify UFC components
Identify the steps of sample processing
Resolve probe clog and aspiration failure messages
Perform clot filter replacement

Welcome
Topics Competencies Completed

Methods Identify the components of the hemoglobin
transmission
Identify cell populations on RBC, Platelet, Retic,
Perox and Baso cytogram
Scheduled Maintenance Perform turning off the system
Perform wipe the shear valve maintenance

Perform automatic hydraulic pathways maintenance
Perform clean Autosampler centering collar
maintenance
Perform semi-automatic vent line wash
Perform replacement of RBC/Baso and Perox Sheath
filters
Perform clean the air-circulation filter maintenance
Record maintenance in the Service Log
Gains Identify when to adjust gains
Identify what to check before adjusting gains
Review the gains adjustment process using the Gains
Wizard
Calibration Identify when to calibrate
Identify what to check before calibrating
Review the calibration process using the Calibration
Wizard
Troubleshooting Utilize the Operator’s Guide to locate morphology
and sample/system flags
Identify troubleshooting basics
Perform shear valve cleaning

Welcome
Observed By: __________________________________________________
Participant: __________________________________________________
Date: __________________________________________________
What was most helpful to you during this program?
How can we improve this program to make it more meaningful to you?

System Overview
2 System Overview

System Overview
System Overview
Resources
• ADVIA 2120i Hematology System Hardware and Software
Online Training Course
• ADVIA 2120/2120i Hematology Systems Operator’s Guide
• ADVIA 2120i Hematology System Quick Reference Guide
Objectives
After completing this exercise, you will be able to:
• Identify system hardware components
• Identify system reagents
• Navigate key areas of the software

System Overview
Identify System Hardware Components

Refer to the ADVIA 2120i Hematology System Quick Reference Guide and identify
component accordingly.
1. Unified Fluids Circuit (UFC)
2. Waste Container
3. Laser Optical Assembly
4. Perox Optical Assembly
5. Autosampler
6. Overflow Bottle
7. Sheath/Rinse
8. Syringe Pump Assembly Left and Right
9. Touchpad
10. Manual Open-Tube Sampler (MOTS)
11. Manual Closed-Tube Sampler (MCTS)
12. Reagents
13. Hgb Optical Assembly

System Overview
Identify System Reagents

1. Which reagent is not on board the analyzer?
2. Which reagents are bundled together?
3. List the reagents on the right side of the instrument
4. List the reagents on the left side of the instrument.
Navigate Key Areas of the Software

1. Identify the following icons located on status line 1.
A B C
A.
B.
C.
2. What is the current status of your analyzer?
3. Select the service icon and note the last date recorded for the Turning off
the System maintenance task.

System Overview
4. Select Info > About. What is the software version listed for this analyzer?
5. In the Data Manager > Sample Control Panel, how many samples are pending?
6. Select Customize > Tools View > Routine Parameters. How do you return to
the Sample Control Panel?
7. Go to Logs > Reagent log. What is the expiration date of the ADVIA
Sheath/Rinse?

Starting Each Shift
3 Starting Each Shift

Starting Each Shift
Starting Each Shift

Resources
Objectives
• List the steps for Starting each Shift
• List the steps to empty waste
• Locate and check the overflow bottle
• Recognize reagent inventory needs and replace if needed
• Evaluate background counts

Starting Each Shift
Starting Each Shift

Use the ADVIA 2120i Hematology System Quick Reference Guide to complete the
following exercises.
Empty the Waste Container and Check the Overflow Bottle

1. Practice emptying the waste.
2. Verify the overflow bottle is empty.
Check the Status of Reagent Inventory: Replace Reagents if

Needed
1. Are any reagents in need of replacement? If so, replace reagents.
2. What two reagents are NOT monitored on cycle by cycle basis?

Hint: From Startup screen, select the Help button. Click on “Check the Reagent
Status.”
3. What reagent must be checked visually each start of shift?
Evaluate the Background Counts

Navigate to Operations > Startup.
1. How can you verify background counts are in range?
2. How does the software display a background count that is out of range?
3. What is your first course of action if the background counts are out of range?
4. If the background counts are still out of range, what would be the next course of
action? Now, perform that action.

Starting Each Shift

Quality Control I
4 Quality Control I

Quality Control I
Quality Control I
Resources
Objectives
• Prepare and perform Quality Control processing
• Review and validate QC results

Quality Control I
Prepare and Run QC

Refer to the ADVIA 2120i Hematology System Quick Reference Guide and the control
package insert to prepare the instrument and control material for processing.
1. Refer to the ADVIA TESTpoint™ package insert to complete the following

statement. Controls are brought to __________ temperature and must be well
__________ before they are run.
2. Which status must the instrument display before you can run controls?
3. Run the provided controls in all three modes.
Review and Validate QC

Refer to ADVIA 2120i Hematology System Quick Reference Guide.
1. Select each control in the list and view the status of the parameters in the Test
Panel.
2. Define the color coding in the Sample Control Panel.

a. Green:
b. Yellow:
c. Red:
3. What does an asterisk (*) in front of a QC control code indicate?
4. Select a control with an asterisk (*). What must be done to validate the control?
5. Which icon did you select to validate the control?

Quality Control I
6. How did the control change in the Sample Control Panel?
7. Print the report from Review/Edit screen for this control.

Sample Processing
5 Sample Processing

Sample Processing
Sample Processing
Resources
• ADVIA 2120i Hematology Systems Quick Reference Guide
Objectives
• Process samples on Autosampler, Manual Open-tube sampler, and Manual
Closed-tube Sampler
• Identify the sample status
• Locate and validate sample results
• Use Manual Sample ID option to enter samples
• Use Order Entry to create workorders

Sample Processing
Process Barcoded Samples on the Autosampler, Manual Open-

Tube Sampler, and Manual Closed-Tube Sampler
1. Process each of the barcoded samples at the workstation using the
Autosampler, MOTS, and MCTS.
2. Process the non-barcoded tube in the MCTS mode.
Identify Sample Status

Navigate to the Data Manager > Sample Control Panel
1. Which sample status now contains the barcoded sample results?
2. Open the unmatched folder. What did you find in the folder?
Review and Validate Sample Results

Navigate to Data Manager > Sample Control Panel. Select Incomplete and scroll
through each patient’s results.
1. Circle the icon that is used to validate sample results?
2. Select the validate button. What sample status now contains newly validated
sample results?

Sample Processing
Order Entry and Manual Sample ID
1. Select Data Manager > Order Entry. Create a patient with a SID# and name and
order a CBC/Diff for one of the non-barcoded samples at your workstation.
Create a second workorder for the other non-barcoded tube provided.
2. Select Operations > Manual Sample ID. With patient data already entered in
Order Entry, enter Sample ID into Manual Sample ID and process using the
MOTS. Repeat process for the other created workorder.
3. Review and validate all processed samples.
4. Obtain printout of final patient reports.
5. Bring final reports to the table.

How the System Works
6 How the System Works


Resources
• ADVIA® 2120i Hematology System How the System Works Online Training: The
Fluidics Process Video
Objectives
• Identify UFC components
• Identify the steps of sample processing
• Resolve probe clog and aspiration failure messages
• Perform clot filter replacement

Unified Fluids Circuit (UFC)
VENT PORTS
HGB
UFC VAC
37 36 14
BASO
PEROX
From
PEROX CFM
16 8 From
RBC/BASO/RETIC
18 13 CFM
To
PEROX 12 To
RBC/BASO/RETIC
VSC
sample syringe
20 sample syringe
5 22
17 6 7
RBC
11
VSC
9
45 21 10
43 33 49
3 72
RETICS
COUPLER
44 SSV
PLATE 31 66
4 73 1
35 2 64
EQUIL
46 74 65
61
47
VA C U U
M WASTE
SSV = sample shear valve

VSC = vacuum shuttle chamber
CFM = concentric flow module

Identify Shuttling Process
1. View The Fluidic Process Video and put the following sequence in the correct
order:
shear valve turns to the dispense position
MCTS needle is rinsed
green Ready light stops flashing and MCTS needle retracts
sample reaches conductivity detector #1
reagents are dispensed
sample reaches conductivity detector #2
Ready light is green
sample is aspirated via MCTS
2. Aspirate a sample in the Manual Closed-tube sampler (MCTS) and observe the
aspiration cycle.
a. Which of the chambers is shuttled to a flowcell via a syringe FIRST?
__________________________________________________________
b. What reaction chamber empties just before the perox chamber is shuttled
to the flowcell via syringe?
__________________________________________________________
c. Which chamber is not shuttled to a flowcell via a syringe?
__________________________________________________________
d. How can you easily identify the Retic chamber?
__________________________________________________________

Troubleshoot Probe Clog / Aspiration Failure Messages
Status-line Message Errors:

Probe Clog
Conductivity detector 1 did not detect a sample within 20 seconds of aspiration.
Aspiration Failed - Probe Clog
Conductivity detector 2 did not detect a sample within the 20 seconds of aspiration.

Replace the Clot Filter

1. Analyzer Mode: Standby
2. Unscrew the filter tube fitting and tube assembly from the front of the selector
valve.
3. Gently push the silicone sleeve to release the clot filter from the filter adapter
fitting.
4. Visually check the filter adapter fitting and the input port of the selector
assembly for any debris or small particles.
5. If you find some debris, use a piece of lens tissue moistened with water to
remove it.
6. Wet the new clot filter with water and place it into the filter adapter fitting.
7. Orientation of the filter is not important.
8. Reconnect the filter adapter fitting to the selector valve. Finger tighten.
Important: Be very careful not to misthread the fitting. Misthreading could strip the
valve which will prevent proper operation.
9. Press Standby to return analyzer to Ready to Run mode.
10. Run a sample and visually check for air bubbles in the sample line between the
selector valve and the UFC block.
Selector valve
Filter tube fitting

Effects of Overtightening the Clot Filter
Normal Clot Filter Overtightened Clot Filter

Methods
7 Methods

Methods
Methods
Resources
Objectives
• Identify components of hemoglobin transmission
• Identify cell populations on the RBC, platelet, retic, baso and perox cytogram

Methods
Hemoglobin Method Exercise
1. Hemoglobin reagent is part of which TIMEPAC®?
2. Identify the five parts of the hemoglobin transmission histogram.
___ Sample reading
___ Sheath/Rinse reading from previous cycle
___ Sheath/rinse Baseline reading
___ Draining and refilling with sample and HGB reagent
___ Draining and refilling with Sheath/Rinse

Methods
RBC Method Exercise
1. Label the RBC V/HC cytogram to indicate where the following cell types are
located.
Microcytic Hyperchromic
Macrocytic Hypochromic
2. Which reagent encases the sample stream as it goes through the RBC flowcell?
Where is this reagent located on the system?

Methods
Platelet Method Exercise
1. Identify the different areas of the platelet cytogram.
___ Platelets
___ RBC ghosts
___ RBC fragments
___ Large Platelets
___ Red cells

Methods
Reticulocyte Method Exercise
1. Identify the cell populations of the Retic Scatter ABS Cytogram.
___ Mature RBC’s
___ Coincidence events
___ Low absorption retics
___ Platelets
___ Medium absorption retics
___ High absorption retics

Methods
Basophil / Lobularity Method Exercise
1. Label the areas of the Baso cytogram.
___ MN (Lymphocyte and Monocyte nuclei)
___ PMN (Neutrophil and Eosinophil nuclei)
___ Basophil
___ Baso Suspect
___ Blast nuclei
___ Saturation
___ Noise

Methods
Peroxidase Method Exercise
1. Identify the areas of the Perox cytogram.
___ Neutrophils
___ Eosinophils
___ Monocytes
___ Lymphocytes and Basophils
___ Large Unstained Cells (LUCs)
___ Platelet clumps
___ Noise
___ nRBC’s

Methods

Quality Control II
8 Quality Control II

Quality Control Il
Quality Control II
Resources
Objectives
• Demonstrate how to import a new lot of QC data
• Interpret QC data
• Demonstrate printing and exporting of a QC report

Quality Control II
Importing New Lot QC
1. Select Customize > System Setup (or Analyzer Setup) > Tools Modify >
Control Dictionary.
2. Insert the mini CD into the computer.

Note: It is possible to use the barcoded sheet that is enclosed in with each level
of control and only import specific parameters. Scan the lot number barcode
located in the upper right hand corner first, followed by the rest of the
parameters. Proceed to Step 3.
3. Select Import
4. A list of controls appears. Select the first control to import by clicking on the
control name, and then click OK.

Quality Control Il
5. If a control file already exists with this name (in this case, CDR Abnormal1),
this window appears. Click on Create.
6. Enter a code name for the control. Refer to examples of code name below
Note: Code names can be up to 6 characters and must be unique from lot
to lot.
7.
A Code Name can be a

combination of control level, lot
number and expiration date.
Examples of Code Names:

JulLow JulN JulHi or 1045L,
2045N, 3045H
8. Select OK

Quality Control II
9. Place a check mark next to Test Panel in order to see the colors on the
Sample Control Panel.
10. Select OK.
11. A list of controls that still need to be imported appears. Select the next
control level and repeat steps 4-9.
12. After all the controls are imported, click Cancel to end importing.
13. Select Exit button on the lower right hand side to exit Tools Modify.

Quality Control Il
Managing Quality Control Data

1. View the Normal QC for your current lot in the table format by selecting Data
Manager > QC. Circle the icon that will display QC in table format.
2. Circle the icon that allows operator to export QC data and print QC data.
Omit / Add a Comment to QC

1. In the Table Format, you have the ability to omit or disposition a QC result from
calculations. Navigate to Data Manager > QC. Select the normal QC and omit
the last QC run by double-clicking on the date and time. Select Omit/Delete
and select OK.
2. A comment can also be added to a QC in table format. Select an abnormal

control and add a comment by typing comment in box and select OK.
Printing and Exporting QC Report

1. You would like to print the monthly statistics for the Normal control of your
current QC. Select the Printer Icon on the Toolbar. On the Print Selection screen
select From and verify the current month is displayed. In the Selection: All
Daily, select Daily Summary for Control. Select the Normal control and press
OK.
2. After printing QC you would like to save or export the QC data to a USB.
Place USB in drive and select Export to file.

Quality Control II

Scheduled Maintenance
9 Scheduled Maintenance

Resources
Objectives
• Perform turning off the system
• Perform wipe the outside of shear valve maintenance
• Perform automatic hydraulic pathways maintenance
• Perform clean Autosampler centering collar maintenance
• Perform semi-automatic vent line wash
• Perform replacement of RBC/Baso and Perox sheath filters
• Perform clean the air-circulation filter maintenance
• Record maintenance in the Service Log

Use the ADVIA 2120i Hematology System Quick Reference Guide to perform the
scheduled maintenance.
Weekly Maintenance
• Turn off the system
• Wipe the outside of shear valve
• Automatic Hydraulic Pathways Wash
• Inspect Autosampler Centering Collar, clean if necessary
Two Weeks Maintenance

• Clean Autosampler Centering Collar
Monthly Maintenance
• Semi-automatic Vent Line Wash
Two Months Maintenance

• Replace RBC/Baso Sheath Filter and Perox Sheath Filter
Six Months Maintenance

• Clean air-circulation filter
Important:
In addition to these scheduled procedures, periodic inspections of the UFC
pathways, vacuum shuttle, and reaction chambers are essential. If you find
buildup or dirt in any of the lines or chambers, clean the line or chamber in
question.
Document Maintenance Performed in the Service Log

Use the ADVIA 2120i Hematology System Quick Reference Guide to document all
scheduled maintenance performed.


Gains
10 Gains

Gains
Gains
Resources
Objectives
• Identify when to adjust gains.
• Identify what to check before adjusting gains.
• Review the gains adjustment process using the Gains Wizard.

Gains
Identify When to Adjust Gains

Gain adjustment is performed on an as-needed basis and in each of the following
cases:
• On installation
• If there is a significant shift in control values after:
o Replacing a critical hydraulic or optical component
o Installing reagents with a different lot number
o When indicated by quality control data, or frequent false positive or false
negative morphology flags
• When verifying that the optical system is functioning properly before calibration
Identify What to Check before Adjusting Gains

Perform the following before adjusting gains:
1. Visually inspect the hydraulics during operation to ensure that they function
properly.
2. Check pressure and vacuum readings at Analyzer Status tab of the Utilities
menu.
3. Perform the weekly maintenance.
Scenario:
It has been 6 months since the last calibration was performed on the instrument.
The regulatory agency College of American Pathologists (CAP) requires calibration of
the hematology analyzer every 6 months. The operator needs to verify that the
optical system is functioning properly and decides to perform the RBC Gains
Adjustment.
The operator has already performed the following:
1. Performed reagent check.
2. Checked the vacuum and pressure readings.
3. Observed shuttling.
4. Performed the weekly maintenance.

Gains
Review the RBC Gains Adjustment Using the Gains

Wizard
Analyzer Mode: Ready to Run
Material Required: ADVIA 120 / 2120 / 2120i OPTIpoint™
• Unopened stability: vial expiration date, when stored between 2-8°C
• Opened stability – one week, when stored between 2-8°C
• Allow OPTIpoint to reach room temperature
• Vortex vigorously for 30-60 seconds
1. Select Procedures > Adjust Gains > Gain Wizard
2. Enter password and select OK (if required).
3. Select RBC / RETIC. The number of aspirations will default to 3.
4. Select Next
5. Select Next
6. Scan the OPTIpoint package insert, beginning with the lot number. Select Import
Barcode to populate fields.
7. Select Next
8. On the Run / Data Review screen, process 3 aspirations of OPTIpoint through
the manual open-tube sampler (MOTS).
9. After all aspirations are complete evaluate data on Run/Data Review screen. A
red check mark in the Apply column will display on each row of data included in
the statistical analysis; to omit data, deselect the red checkmark.
Note: To run additional aspirations, select Run More and follow on screen
instructions.
10. Select Next and review information on the Gain Acceptance screen. If status is
Passed, select Accept Gains
11. Before final gain acceptance, a final verification is required. Select Ok and
perform the required verification run.
Process 3 aspirations of OPTIpoint through the manual open-tube sampler
(MOTS).
12. After all verification run aspirations are complete, review Verification Run:
Run/Data Review data. A red check mark in the Apply column will display on
each row of data included in the statistical analysis; to omit data, deselect the
red checkmark.
13. For final acceptance, select Next
14. Review information on the Gain Acceptance screen. If status is Passed, select
Finish

Gains
15. Select Gain Factors Log to view and print gain factors.
16. Select Exit to close.
Gain Acceptance Criteria

Number of Points Flatness Factor Range
RBC/Retic 3 n/a 20 - 255
Perox 8 - 25 ≤ 3.2 20 - 255
Baso 5 - 25 ≤ 2.5 20 - 255

Calibration
11 Calibration

Calibration
Calibration
Resources
• ADVIA 2120i Hematology System Calibration Online Training
Objectives
• Identify when to calibrate
• Identify what to check before calibrating
• Review the calibration process using the Calibration Wizard

Calibration
Calibration Overview
When to calibrate
• On installation
• If there is a significant shift in control values after:
o Replacing a critical hydraulic or optical component
o Installing reagents with a different lot number
• Any time commercial control products and/or moving averages are out of range
and you have verified that the out-of-control condition is not instrument related
• At periodic time intervals determined by laboratory regulations
What to check before calibrating
1. Verify that there is sufficient reagent for the calibration. Do not change the
reagent lot number during the calibration.
2. Visually inspect the hydraulics during the operation to ensure that they function
properly.
3. Check pressure and vacuum readings at the Analyzer Status tab of the Utilities
menu.
4. Perform the weekly maintenance.
5. Verify that the optical system is functioning properly.
Note: Do not calibrate unless the gain and alignment of all the optics channels
have been verified.
Materials required for calibration
1. ADVIA SETpoint™ Calibrator and/or 1 whole blood sample to calibrate all

parameters except for the %RETIC.
2. Include 5 whole blood samples if you want to calibrate the %RETIC. %Retic
calibration is only performed at laboratories using a manual counting method
other than those recommended by NCCLS or ICSH, or laboratories using certain
flow cytometric methods. These laboratories could observe a significant bias
when comparing to the ADVIA 120/2120/2120i Hematology System.

Calibration
Calibration Wizard
To access Calibration Wizard, go to ADVIA 2120i Calibration Online Training: Using
the Calibration Wizard.
1. Which sampler type is selected by default in the Calibration Definition screen?
2. Review the Material Required screen.

a. List the two materials required for calibration when calibrating all
parameters with Retic.
b. How do you prepare and handle your calibration materials?
3. Select Next to proceed to the Calibration Reference Data screen.
Review and Accept Calibration Data
1. Calibration Data Review screen
a. Why is it important to print the data before accepting calibration?
b. If a parameter failed, how are data points omitted?
c. What is the minimum number of data points required for Passed

Status?
2. Select the back icon to Access the Calibration Acceptance screen.

What is your calibration status?

Calibration
Locate and Print Calibration Factor Log
1. Go to your instrument and navigate to Calibration screen. Press Cal Factor to

see current and previous Cal Factors.
2. After accepting the calibration, what should you run to verify system
performance?

Troubleshooting
12 Troubleshooting

Troubleshooting
Troubleshooting
Resources
Objectives
• Utilize the Operator’s Guide to locate morphology and sample/system flags
• Identify troubleshooting basics
• Perform shear valve cleaning

Troubleshooting
Troubleshooting Basics
Isolate the Problem
• Observation: leaks, shuttling, bubbles, cytograms, and histograms
• Startup Screen: reagent and background counts
• Analyzer Status: pressures, vacuum, temperature, light source intensity
• Message Log: view past messages for repeated errors
• QC: what individual test parameters are out of range?
• Flags
o Sample/system flags are codes displayed in the Review/Edit tab and the
Run Screen. These codes are associated with a specific test parameter
marked by an asterisk. Isolated instances of flags are usually sample
related. However, multiple occurrences, especially for consecutive
samples, can indicate an analyzer problem.
o Morphology flags are plus signs (+) displayed in the Review/Edit tab and
the Run Screen. These flags, one to three plus signs, alert the operator
to possible cellular conditions that may require additional laboratory
attention, such as preparing a slide for microscopic examination.
o System messages are displayed on the status lines on the PC monitor.
Along with the messages, color-coded icons indicate the severity level of
the message.
General Troubleshooting Steps

1. Verify the following
• Reagent volume
• Reagent delivery
• Reagent in the correct position and straw is submerged in reagent
• All fittings between reagent container and reagent diaphragm pump are
connected
• Prime reagent lines and observe for air bubbles. Air bubbles present may
indicate faulty reagent straw; replace straw
• Excessive bubbles in pathways may indicate a faulty reagent pump. Contact
Siemens Service personnel
2. Verify Sheath Reagent (ADVIA 120 / 2120 / 2120i Sheath/Rinse or Perox Sheath)
delivery by checking sheath filter is 90% full and free of air bubbles.
If bubbles are present, check filter for cracks or leaks in Luer fittings. Replace filter if
needed taking care not to over-tighten Luer fitting.

Troubleshooting
System Flags Troubleshooting
Normal Flow Rates

• Right side of UFC:
IFR Flags: BASOIFR, RBCIFR, RETICIFR

• Left side of UFC:
IFR Flag: PXIFR

• Top part of UFC:
IFR Flag: HGBIFR

Troubleshooting
Irregular Flow Rate Examples

Poor Sheath delivery
Poor sample delivery
Air in sample stream
Improper syringe plunger maintenance
Air in Hemoglobin reagent line
Refer to the ADVIA® 2120i Hematology System Quick Reference Guide:

System Flags Troubleshooting Chart for troubleshooting IFR flags.

Troubleshooting
Clean the Shear Valve

1. Analyzer mode: Off
Important: Place paper towels directly under the shear valve to prevent fluid from
dripping down into the analyzer.
2. Remove the knurled nut by turning counter-clockwise (1).
3. Remove the compression spring (2).
4. Remove the rotor (3).
5. Separate and remove shear face (4) by gently rotating front shear face.
Caution: Do not loosen or remove with sharp object
6. Place the front shear face in 6% sodium hypochlorite solution and soak ten
minutes. Rinse with DI water.
7. Rinse the rear shear face using a wash bottle filled with distilled water.
Caution: Do not use paper towel, gauze or cotton swabs on shear faces
8. Reassemble the shear valve with the faces wet. Verify the black line on the front
face aligns with the black line and A on back face. Smaller loops are at 9 and 11
o’clock and the large loop is at 5 o’clock.
9. Install the rotor by installing the drive pin in the hole on the right side of the front
face.
10. Replace the spring and knurled nut.
11. Hand tighten nut.
12. Turn analyzer power on and verify Background Counts.
13. Run a whole blood primer and QC as per laboratory policy.

Troubleshooting

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ADVIA® 2120i Hematology System

ADVIA® 2120i Hematology System

ADVIA® 2120i Hematology System T02006.005 Effective Date: 02/22/2021

All other trademarks are the property of their respective owners.

ii ADVIA® 2120i Hematology System

3: Starting Each Shift

6: How the System Works

ADVIA® 2120i Hematology System iii

ADVIA® 2120i Hematology System 1-1

1-2 ADVIA® 2120i Hematology System

ADVIA® 2120i Hematology System 1-3

ADVIA® 2120i Hematology System Virtual Training

Topics Competencies Completed

1-4 ADVIA® 2120i Hematology System

Topics Competencies Completed

Perform wipe the shear valve maintenance

Identify troubleshooting basics

Perform shear valve cleaning

ADVIA® 2120i Hematology System 1-5

Observed By: __________________________________________________

What was most helpful to you during this program?

How can we improve this program to make it more meaningful to you?

1-6 ADVIA® 2120i Hematology System

ADVIA® 2120i Hematology System 2-1

2-2 ADVIA® 2120i Hematology System

Identify System Hardware Components

1. Unified Fluids Circuit (UFC)

3. Laser Optical Assembly

4. Perox Optical Assembly

8. Syringe Pump Assembly Left and Right

10. Manual Open-Tube Sampler (MOTS)

11. Manual Closed-Tube Sampler (MCTS)

13. Hgb Optical Assembly

ADVIA® 2120i Hematology System 2-3

Identify System Reagents

2. Which reagents are bundled together?

3. List the reagents on the right side of the instrument

4. List the reagents on the left side of the instrument.

Navigate Key Areas of the Software

2. What is the current status of your analyzer?

2-4 ADVIA® 2120i Hematology System

ADVIA® 2120i Hematology System 2-5

3 Starting Each Shift

ADVIA® 2120i Hematology System 3-1

Starting Each Shift

3-2 ADVIA® 2120i Hematology System

Starting Each Shift

Empty the Waste Container and Check the Overflow Bottle

Check the Status of Reagent Inventory: Replace Reagents if

2. What two reagents are NOT monitored on cycle by cycle basis?

3. What reagent must be checked visually each start of shift?

Evaluate the Background Counts

ADVIA® 2120i Hematology System 3-3

3-4 ADVIA® 2120i Hematology System

ADVIA® 2120i Hematology System 4-1

4-2 ADVIA® 2120i Hematology System

Prepare and Run QC

1. Refer to the ADVIA TESTpoint™ package insert to complete the following

3. Run the provided controls in all three modes.

Review and Validate QC

2. Define the color coding in the Sample Control Panel.

3. What does an asterisk (*) in front of a QC control code indicate?

5. Which icon did you select to validate the control?

ADVIA® 2120i Hematology System 4-3