Sequence: RB1 (Linear / 476 8 bases)

Features: 82 total
Feature Location Size Type

✓ RB1 1 .. 4768 4768 bases gene

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = RB trans c riptional c orepres s or 1
/db_xref = G eneI D : 5 9 2 5
/db_xref = H G N C : H G N C :9 8 8 4
/db_xref = MIM: 614041
source 1 .. 4768 4768 bases source

/chromosome = 13
/map = 1 3 q1 4 .2
/mol_type = mRN A
/db_xref = taxon: 9 6 0 6
/organism = H omo s apiens

✓ exon 1 .. 299 299 bases exon

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/inference = alignment: s ame s pec ies : 2 .1 .0
✓ upstream in-frame stop codon 61 .. 63 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = ups tream in-frame s top c odon

✓ RB1 163 .. 2949 2787 bases CDS

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/product = retinoblas toma-as s oc iated protein is oform 1
/note = is oform 1 is enc oded by trans c ript variant 1 ; retinoblas toma-as s oc iated protein; prepro- retinoblas toma- as s oc iated protein; protein
phos phatas e 1 , regulatory s ubunit 1 3 0 ; retinoblas toma s us pec tibility protein; retinoblas toma 1
/db_xref = C C D S: C C D S3 1 9 7 3 .1
/db_xref = G eneI D : 5 9 2 5
/db_xref = H G N C : H G N C :9 8 8 4
/db_xref = MIM: 614041
/protein_id = N P _0 0 0 3 1 2 .2
/translation = MPPKTPRKTAATAAAAAAEPPAPPPPPPPEEDPEQDSGPEDLPLVRLEFEETEEPDFTALCQKLKIPDHVRERAWLTWEKVSSVDGVLGGYIQKKKELWGICIFIAAVDL
DEMSFTFTELQKNIEISVHKFFNLLKEIDTSTKVDNAMSRLLKKYDVLFALFSKLERTCELIYLTQPSSSISTEINSALVLKVSWITFLLAKGEVLQMEDDLVISFQLML
CVLDYFIKLSPPMLLKEPYKTAVIPINGSPRTPRRGQNRSARIAKQLENDTRIIEVLCKEHECNIDEVKNVYFKNFIPFMNSLGLVTSNGLPEVENLSKRYEEIYLKNKD
LDARLFLDHDKTLQTDSIDSFETQRTPRKSNLDEEVNVIPPHTPVRTVMNTIQQLMMILNSASDQPSENLISYFNNCTVNPKESILKRVKDIGYIFKEKFAKAVGQGCVE
IGSQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSTSQNLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKAEGNLTREMIKHL
ERCEHRIMESLAWLSDSPLFDLIKQSKDREGPTDHLESACPLNLPLQNNHTAADMYLSPVRSPKKKGSTTRVNSTANAETQATSAFQTQKPLKSTSLSLFYKKVYRLAYL
RLNTLCERLLSEHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAVQETFKRVLIKEEEYDSIIVFYNSVFMQRLKTNILQ
YASTRPPTLSPIPHIPRSPYKFPSSPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMVCNSDRVLKRSAEGSNPPKPLKKLRFDIE
GSDEADGSKHLPGESKFQQKLAEMTSTRTRMQKQKMNDSMDTSNKEEK*
928 amino acids = 106.2 kDa
✓ miscellaneous 163 .. 288 126 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = propagated from U niP rotKB/Swis s -P rot (P 0 6 4 0 0 .2 );
Region: D is ordered. /evidenc e=E C O :0 0 0 0 2 5 6 |SA M :M obiD B -lite
✓ miscellaneous 166 .. 168 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = N ,N -dimethylproline. /evidenc e=E C O :0 0 0 0 2 5 0 |U niP rotKB :P 1 3 4 0 5 ; propagated from U niP rotKB/Swis s -P rot (P 0 6 4 0 0 .2 );
methylation s ite

✓ miscellaneous 271 .. 273 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = P hos phos erine.
/evidenc e=E C O :0 0 0 7 7 4 4 |P ubM ed:1 8 6 6 9 6 4 8 , E C O :0 0 0 7 7 4 4 |P ubM ed:1 9 6 9 0 3 3 2 , E C O :0 0 0 7 7 4 4 |P ubM ed:2 4 2 7 5 5 6 9 ;
propagated from U niP rotKB/Swis s -P rot (P 0 6 4 0 0 .2 ); phos phorylation s ite

Print ed from SnapGene®: 18 Oc t 2022 20:39 Page 1

 RB1 (Linear / 476 8 bases)

Feature Location Size Type

✓ exon 300 .. 426 127 bases exon

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/inference = alignment: s ame s pec ies : 2 .1 .0
✓ exon 427 .. 542 116 bases exon

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/inference = alignment: s ame s pec ies : 2 .1 .0

✓ miscellaneous 502 .. 843 342 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = D omain of unknown func tion (D U F 3 4 5 2 ); Region:
D U F3 4 5 2 ; pfam1 1 9 3 4
/db_xref = C DD: 432200

✓ exon 543 .. 662 120 bases exon

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/inference = alignment: s ame s pec ies : 2 .1 .0
✓ exon 663 .. 701 39 bases exon

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/inference = alignment: s ame s pec ies : 2 .1 .0

✓ exon 702 .. 769 68 bases exon

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/inference = alignment: s ame s pec ies : 2 .1 .0
✓ exon 770 .. 880 111 bases exon

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/inference = alignment: s ame s pec ies : 2 .1 .0

✓ exon 881 .. 1023 143 bases exon

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/inference = alignment: s ame s pec ies : 2 .1 .0
✓ miscellaneous 907 .. 909 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = P hos phos erine, by C D K1 . /evidenc e=E C O :0 0 0 0 2 6 9 |P ubM ed:1 7 5 6 7 3 5 , E C O :0 0 0 7 7 4 4 |P ubM ed:1 8 2 2 0 3 3 6 , E C O :0 0 0 7 7 4 4 |
P ubM ed:1 8 6 6 9 6 4 8 , E C O :0 0 0 7 7 4 4 |P ubM ed:1 9 6 9 0 3 3 2 , E C O :0 0 0 7 7 4 4 |P ubM ed:2 1 4 0 6 6 9 2 , E C O :0 0 0 7 7 4 4 |P ubM ed:
2 3 1 8 6 1 6 3 ; propagated from U niP rotKB /Swis s -P rot (P 0 6 4 0 0 .2 ); phos phorylation s ite
✓ miscellaneous 916 .. 918 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = P hos phothreonine, by C D K1 . /evidenc e=E C O :0 0 0 0 2 6 9 |P ubM ed:1 7 5 6 7 3 5 , E C O :0 0 0 7 7 4 4 |P ubM ed:1 8 2 2 0 3 3 6 , E C O :0 0 0 7 7 4 4 |
P ubM ed:1 8 6 6 9 6 4 8 , E C O :0 0 0 7 7 4 4 |P ubM ed:2 3 1 8 6 1 6 3 ; propagated from U niP rotKB/Swis s -P rot (P 0 6 4 0 0 .2 ); phos phorylation s ite
✓ exon 1024 .. 1101 78 bases exon

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/inference = alignment: s ame s pec ies : 2 .1 .0

Print ed from SnapGene®: 18 Oc t 2022 20:39 Page 2

 RB1 (Linear / 476 8 bases)

Feature Location Size Type

✓ exon 1102 .. 1211 110 bases exon

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/inference = alignment: s ame s pec ies : 2 .1 .0
✓ exon 1212 .. 1289 78 bases exon

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/inference = alignment: s ame s pec ies : 2 .1 .0

✓ miscellaneous 1228 .. 1230 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = P hos phothreonine.
/evidenc e=E C O :0 0 0 7 7 4 4 |P ubM ed:1 8 2 2 0 3 3 6 , E C O :0 0 0 7 7 4 4 |P ubM ed:2 3 1 8 6 1 6 3 ; propagated from U niP rotKB/Swis s - P rot
(P 0 6 4 0 0 .2 ); phos phorylation s ite

✓ miscellaneous 1279 .. 2475 1197 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = propagated from U niP rotKB/Swis s -P rot (P 0 6 4 0 0 .2 );
Region: P oc ket, binds T and E 1 A

✓ miscellaneous 1279 .. 1899 621 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = propagated from U niP rotKB/Swis s -P rot (P 0 6 4 0 0 .2 );
Region: D omain A
✓ miscellaneous 1279 .. 1881 603 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = Retinoblas toma- as s oc iated protein A domain; Region:
RB_A ; pfam0 1 8 5 8
/db_xref = C DD: 426479
✓ miscellaneous 1279 .. 1281 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = P hos phothreonine, by C D K1 . /evidenc e=E C O :0 0 0 0 2 6 9 |P ubM ed:1 7 5 6 7 3 5 , E C O :0 0 0 7 7 4 4 |P ubM ed:1 9 6 9 0 3 3 2 , E C O :0 0 0 7 7 4 4 |
P ubM ed:2 3 1 8 6 1 6 3 ; propagated from U niP rotKB/Swis s -P rot (P 0 6 4 0 0 .2 ); phos phorylation s ite
✓ exon 1290 .. 1377 88 bases exon

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/inference = alignment: s ame s pec ies : 2 .1 .0

✓ exon 1378 .. 1494 117 bases exon

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/inference = alignment: s ame s pec ies : 2 .1 .0
✓ exon 1495 .. 1551 57 bases exon

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/inference = alignment: s ame s pec ies : 2 .1 .0

✓ exon 1552 .. 1583 32 bases exon

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/inference = alignment: s ame s pec ies : 2 .1 .0

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 RB1 (Linear / 476 8 bases)

Feature Location Size Type

✓ exon 1584 .. 1660 77 bases exon

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/inference = alignment: s ame s pec ies : 2 .1 .0
✓ exon 1661 .. 1857 197 bases exon

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/inference = alignment: s ame s pec ies : 2 .1 .0

✓ exon 1858 .. 1976 119 bases exon

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/inference = alignment: s ame s pec ies : 2 .1 .0
✓ miscellaneous 1861 .. 1863 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = P hos phos erine, by C D K2 . /evidenc e=E C O :0 0 0 0 2 6 9 |P ubM ed:1 0 4 9 9 8 0 2 ; propagated from U niP rotKB/S wis s -P rot (P 0 6 4 0 0 .2 );
phos phorylation s ite
✓ miscellaneous 1900 .. 2079 180 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = propagated from U niP rotKB/Swis s -P rot (P 0 6 4 0 0 .2 );
Region: Spac er

✓ exon 1977 .. 2122 146 bases exon

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/inference = alignment: s ame s pec ies : 2 .1 .0
✓ miscellaneous 1984 .. 1986 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = P hos phos erine.
/evidenc e=E C O :0 0 0 0 2 5 0 |U niP rotKB:P 1 3 4 0 5 ; propagated from U niP rotKB /Swis s -P rot (P 0 6 4 0 0 .2 ); phos phorylation s ite
✓ miscellaneous 1990 .. 2058 69 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = propagated from U niP rotKB/Swis s -P rot (P 0 6 4 0 0 .2 );
Region: D is ordered. /evidenc e=E C O :0 0 0 0 2 5 6 |SA M :M obiD B -lite

✓ miscellaneous 1996 .. 1998 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = P hos phos erine, by C H E K2 and C H E K1 . /evidenc e=E C O :0 0 0 0 2 6 9 |P ubM ed:1 7 3 8 0 1 2 8 , E C O :0 0 0 7 7 4 4 |P ubM ed:1 8 6 6 9 6 4 8 ;
propagated from U niP rotKB/Swis s -P rot (P 0 6 4 0 0 .2 ); phos phorylation s ite

✓ miscellaneous 2032 .. 2034 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = P hos phos erine.
/evidenc e=E C O :0 0 0 7 7 4 4 |P ubM ed:2 3 1 8 6 1 6 3 ; propagated from U niP rotKB/S wis s - P rot (P 0 6 4 0 0 .2 ); phos phorylation s ite
✓ miscellaneous 2080 .. 2475 396 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = propagated from U niP rotKB/Swis s -P rot (P 0 6 4 0 0 .2 );
Region: D omain B

Print ed from SnapGene®: 18 Oc t 2022 20:39 Page 4

 RB1 (Linear / 476 8 bases)

Feature Location Size Type

✓ miscellaneous 2095 .. 2475 381 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = c yc lin box found in retinoblas toma- as s oc iated
protein (RB) and s imilar proteins ; Region: C Y C L I N _RB; c d2 0 5 9 9
/db_xref = C DD: 410302

✓ miscellaneous 2095 .. 2097 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = E 2 F trans c ription fac tor binding s ite [polypeptide binding]; other s ite
/db_xref = C DD: 410302

✓ miscellaneous 2107 .. 2109 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = E 2 F trans c ription fac tor binding s ite [polypeptide binding]; other s ite
/db_xref = C DD: 410302
✓ miscellaneous 2116 .. 2121 6 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = E 2 F trans c ription fac tor binding s ite [polypeptide binding]; other s ite
/db_xref = C DD: 410302
✓ exon 2123 .. 2268 146 bases exon

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/inference = alignment: s ame s pec ies : 2 .1 .0

✓ miscellaneous 2128 .. 2130 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = E 2 F trans c ription fac tor binding s ite [polypeptide binding]; other s ite
/db_xref = C DD: 410302
✓ miscellaneous 2140 .. 2142 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = E 2 F trans c ription fac tor binding s ite [polypeptide binding]; other s ite
/db_xref = C DD: 410302
✓ exon 2269 .. 2373 105 bases exon

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/inference = alignment: s ame s pec ies : 2 .1 .0

✓ miscellaneous 2287 .. 2289 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = L xC xE peptide binding s ite [polypeptide binding];
other s ite
/db_xref = C DD: 410302

✓ miscellaneous 2299 .. 2304 6 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = L xC xE peptide binding s ite [polypeptide binding];
other s ite
/db_xref = C DD: 410302

Print ed from SnapGene®: 18 Oc t 2022 20:39 Page 5

 RB1 (Linear / 476 8 bases)

Feature Location Size Type

✓ miscellaneous 2320 .. 2325 6 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = L xC xE peptide binding s ite [polypeptide binding];
other s ite
/db_xref = C DD: 410302

✓ exon 2374 .. 2487 114 bases exon

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/inference = alignment: s ame s pec ies : 2 .1 .0
✓ miscellaneous 2419 .. 2421 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = L xC xE peptide binding s ite [polypeptide binding];
other s ite
/db_xref = C DD: 410302
✓ miscellaneous 2428 .. 2433 6 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = L xC xE peptide binding s ite [polypeptide binding];
other s ite
/db_xref = C DD: 410302
✓ miscellaneous 2443 .. 2445 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = L xC xE peptide binding s ite [polypeptide binding];
other s ite
/db_xref = C DD: 410302
✓ miscellaneous 2449 .. 2946 498 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = propagated from U niP rotKB/Swis s -P rot (P 0 6 4 0 0 .2 );
Region: I nterac tion with L I M D 1 . /evidenc e=E C O :0 0 0 0 2 6 9 |P ubM ed:1 5 5 4 2 5 8 9
✓ miscellaneous 2464 .. 2937 474 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = Rb C -terminal domain; Region: Rb_C ; pfam0 8 9 3 4
/db_xref = C DD: 430328

✓ miscellaneous 2473 .. 2946 474 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = propagated from U niP rotKB/Swis s -P rot (P 0 6 4 0 0 .2 );
Region: D omain C , mediates interac tion with E 4 F1 . /evidenc e=E C O :0 0 0 0 2 6 9 |P ubM ed:1 0 8 6 9 4 2 6

✓ exon 2488 .. 2651 164 bases exon

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/inference = alignment: s ame s pec ies : 2 .1 .0
✓ miscellaneous 2500 .. 2502 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = P hos phos erine.
/evidenc e=E C O :0 0 0 7 7 4 4 |P ubM ed:2 3 1 8 6 1 6 3 ; propagated from U niP rotKB/S wis s - P rot (P 0 6 4 0 0 .2 ); phos phorylation s ite

Print ed from SnapGene®: 18 Oc t 2022 20:39 Page 6

 RB1 (Linear / 476 8 bases)

Feature Location Size Type

✓ miscellaneous 2524 .. 2526 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = P hos phos erine.
/evidenc e=E C O :0 0 0 7 7 4 4 |P ubM ed:2 3 1 8 6 1 6 3 ; propagated from U niP rotKB/S wis s - P rot (P 0 6 4 0 0 .2 ); phos phorylation s ite

✓ miscellaneous 2545 .. 2547 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = P hos phos erine.
/evidenc e=E C O :0 0 0 7 7 4 4 |P ubM ed:2 3 1 8 6 1 6 3 ; propagated from U niP rotKB/S wis s - P rot (P 0 6 4 0 0 .2 ); phos phorylation s ite
✓ miscellaneous 2581 .. 2583 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = P hos phos erine, by C D K1 and C D K3 . /evidenc e=E C O :0 0 0 0 2 6 9 | P ubM ed:1 5 0 8 4 2 6 1 , E C O :0 0 0 0 2 6 9 | P ubM ed:1 7 5 6 7 3 5 , E C O :
0 0 0 7 7 4 4 |P ubM ed:1 8 6 6 9 6 4 8 , E C O :0 0 0 7 7 4 4 |P ubM ed:1 9 6 9 0 3 3 2 , E C O :0 0 0 7 7 4 4 |P ubM ed:2 3 1 8 6 1 6 3 ; propagated from
U niP rotKB/Swis s -P rot (P 0 6 4 0 0 .2 ); phos phorylation s ite
✓ miscellaneous 2590 .. 2592 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = N 6 -methyllys ine, by SM Y D 2 . /evidenc e=E C O :0 0 0 0 2 6 9 | P ubM ed:2 2 7 8 7 4 2 9 ; propagated from U niP rotKB/S wis s -P rot (P 0 6 4 0 0 .2 );
methylation s ite
✓ miscellaneous 2593 .. 2595 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = P hos phos erine, by C D K1 and C D K3 . /evidenc e=E C O :0 0 0 0 2 6 9 | P ubM ed:1 5 0 8 4 2 6 1 , E C O :0 0 0 0 2 6 9 | P ubM ed:1 7 5 6 7 3 5 , E C O :
0 0 0 7 7 4 4 |P ubM ed:1 8 6 6 9 6 4 8 , E C O :0 0 0 7 7 4 4 |P ubM ed:2 3 1 8 6 1 6 3 ; propagated from U niP rotKB/Swis s - P rot (P 0 6 4 0 0 .2 );
phos phorylation s ite
✓ miscellaneous 2623 .. 2625 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = P hos phothreonine, by C D K6 . /evidenc e=E C O :0 0 0 0 2 6 9 |P ubM ed:1 5 8 0 9 3 4 0 , E C O :0 0 0 0 2 6 9 |P ubM ed:1 6 3 6 0 0 3 8 , E C O :0 0 0 7 7 4 4 |
P ubM ed:2 0 0 6 8 2 3 1 , E C O :0 0 0 7 7 4 4 |P ubM ed:2 3 1 8 6 1 6 3 ; propagated from U niP rotKB/Swis s -P rot (P 0 6 4 0 0 .2 ); phos phorylation s ite

✓ miscellaneous 2629 .. 2631 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = P hos phothreonine.
/evidenc e=E C O :0 0 0 7 7 4 4 |P ubM ed:1 8 6 6 9 6 4 8 , E C O :0 0 0 7 7 4 4 |P ubM ed:2 3 1 8 6 1 6 3 ; propagated from U niP rotKB/Swis s - P rot
(P 0 6 4 0 0 .2 ); phos phorylation s ite

✓ miscellaneous 2638 .. 2640 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = P hos phothreonine, by C D K4 . /evidenc e=E C O :0 0 0 0 2 6 9 |P ubM ed:1 5 8 0 9 3 4 0 , E C O :0 0 0 0 2 6 9 |P ubM ed:1 6 3 6 0 0 3 8 , E C O :0 0 0 7 7 4 4 |
P ubM ed:1 8 6 6 9 6 4 8 , E C O :0 0 0 7 7 4 4 |P ubM ed:2 0 0 6 8 2 3 1 , E C O :0 0 0 7 7 4 4 |P ubM ed:2 3 1 8 6 1 6 3 ; propagated from U niP rotKB/Swis s -
P rot (P 0 6 4 0 0 .2 ); phos phorylation s ite

✓ exon 2652 .. 2682 31 bases exon

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/inference = alignment: s ame s pec ies : 2 .1 .0
✓ exon 2683 .. 2825 143 bases exon

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/inference = alignment: s ame s pec ies : 2 .1 .0

Print ed from SnapGene®: 18 Oc t 2022 20:39 Page 7

 RB1 (Linear / 476 8 bases)

Feature Location Size Type

✓ miscellaneous 2683 .. 2685 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = P hos phothreonine.
/evidenc e=E C O :0 0 0 7 7 4 4 |P ubM ed:1 9 6 9 0 3 3 2 ; propagated from U niP rotKB/S wis s - P rot (P 0 6 4 0 0 .2 ); phos phorylation s ite

✓ miscellaneous 2725 .. 2727 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = P hos phos erine.
/evidenc e=E C O :0 0 0 7 7 4 4 |P ubM ed:2 3 1 8 6 1 6 3 ; propagated from U niP rotKB/S wis s - P rot (P 0 6 4 0 0 .2 ); phos phorylation s ite
✓ miscellaneous 2740 .. 2946 207 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = propagated from U niP rotKB/Swis s -P rot (P 0 6 4 0 0 .2 );
Region: D is ordered. /evidenc e=E C O :0 0 0 0 2 5 6 |SA M :M obiD B -lite
✓ miscellaneous 2740 .. 2790 51 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = propagated from U niP rotKB/Swis s -P rot (P 0 6 4 0 0 .2 );
Region: Bipartite nuc lear loc alization s ignal. /evidenc e=E C O :0 0 0 0 2 5 0 |U niP rotKB:P 1 3 4 0 5

✓ miscellaneous 2740 .. 2742 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = N 6 -methyllys ine, by SM Y D 2 . /evidenc e=E C O :0 0 0 0 2 6 9 | P ubM ed:2 0 8 7 0 7 1 9 ; propagated from U niP rotKB/S wis s -P rot (P 0 6 4 0 0 .2 );
methylation s ite

✓ miscellaneous 2779 .. 2781 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = N 6 -ac etyllys ine, by P C A F. /evidenc e=E C O :0 0 0 0 2 6 9 | P ubM ed:2 0 9 4 0 2 5 5 ; propagated from U niP rotKB/S wis s - P rot (P 0 6 4 0 0 .2 );
ac etylation s ite
✓ miscellaneous 2782 .. 2784 3 bases misc_feature

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = N 6 -ac etyllys ine, by P C A F. /evidenc e=E C O :0 0 0 0 2 6 9 | P ubM ed:2 0 9 4 0 2 5 5 ; propagated from U niP rotKB/S wis s - P rot (P 0 6 4 0 0 .2 );
ac etylation s ite
✓ exon 2826 .. 2875 50 bases exon

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/inference = alignment: s ame s pec ies : 2 .1 .0

✓ exon 2876 .. 4768 1893 bases exon

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/inference = alignment: s ame s pec ies : 2 .1 .0
✓ hexamer: AATAAA 4743 .. 4748 6 bases regulatory

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = hexamer: A ATA A A
/regulatory_class = polyA _s ignal_s equenc e

✓ major polyA site 4768 .. 4768 1 base polyA_site

/gene = RB1
/gene_synonym = O SRC ; p1 0 5 -Rb; p1 1 0 -RB1 ; pp1 1 0 ; P P P 1 R1 3 0 ;
pRb; RB
/note = major polyA s ite

Print ed from SnapGene®: 18 Oc t 2022 20:39 Page 8