1.

Capillary Electrophoresis: Principles and Instrumentation

2. Requirements for STR Typing

3. Principles and Components of Capillary Electrophoresis

4. Electrokinetic Injection

4.1 Sample Preparation

5. DNA Separation

5.1 Polymer Solution

5.2 Buffer

5.3 The Capillary

5.4 Factors Impacting Resolution

6. Fluorescence Detection

6.1 Optics for Genetic Analyzers

6.2 Fluorescent Dyes and Emission Filter Sets

6.3 Spectral Calibration

6.4 Adjusting Dye Sensitivities with Variable Binning

7.0 Applications of forensic DNA testing

7.1 Forensic casework

7.2 DNA databasing

8.0 Sample interpretation

8.1 Software used

8.2 Assessing resolution of DNA separations

9. Instrumentation

9.1 Overview of Sample Processing Steps

9.2 The ABI Prism 310 Genetic Analyzer

9.3 ABI 3100 and ABI 3130xl Instruments

9.4 ABI PRISM 3500 and 3500xl Genetic Analyzers

1.0 Capillary Electrophoresis: Principles and Instrumentation 6.3 Spectral Calibration

2.0 Requirements for STR Typing 6.4 Adjusting Dye Sensitivities with Variable Binning

3.0 Principles and Components of Capillary Electrophoresis 7.0 Sample interpretation

4.0 Electrokinetic Injection 7.1 Software used

4.1 Sample Preparation 7.2 Assessing resolution of DNA separations

5.0 DNA Separation 8.0 Applications of forensic DNA testing

5.1 Polymer Solution 8.1 Forensic casework

5.2 Buffer 8.2 DNA databasing

5.3 The Capillary 9.0 Instrumentation

5.4 Factors Impacting Resolution 9.1 Overview of Sample Processing Steps

6.0 Fluorescence Detection 9.2 The ABI Prism 310 Genetic Analyzer

6.1 Optics for Genetic Analyzers 9.3 ABI 3100 and ABI 3130xl Instruments

6.2 Fluorescent Dyes and Emission Filter Sets 9.4 ABI PRISM 3500 and 3500xl Genetic Analyzers