Polyamine Metabolism

Polyamine Metabolism
 DNA Damage Repair

(mol/mmol creatinine)
30
(mol/mmol creatinine)

60 #
* #

N1-Acetylspermidine
Sham Sham
# # Rad 4
* Rad 4
Deoxyuridine

40 * # 20
*
* *
20
* 10
*

0 0
D-2
D0
D2

D-2
D0
D2

D-2
D0
D2

D-2
D0
D2

D-2
D0
D2

D-2
D0
D2

D-2
D0
D2

D-2
D0
D2
2 mon 4 mon 6 mon 8 mon 2 mon 4 mon 6 mon 8 mon

2 months 4 months 8 months

R2 = 0.66
R2 = 0.86 R2 = 0.87
P < 0.03
P < 0.003 P < 0.003

N1-Acetylspermidine (M)
N1-Acetylspermidine (M)

N1-Acetylspermidine (M)

80 100 60

80
60
40
60
40
40
20
20
20

0 0 0
0 100 200 300 400 500 0 200 400 600 800 0 100 200 300 400 500
Deoxyuridine (M) Deoxyuridine (M) Deoxyuridine (M)
Aortic Aneurysm

Liu et al (2022) Nutrients

Microbial Co-metabolism
 Regulation of proliferation

Nakamura et al (2021) Nat Comm

 Regulation of macrophage development and Colitis

Nakamura et al (2021) Nat Comm

Glycolysis and Cancer
 Glycolysis and Cancer

Warburg Effect:

 Neoplastic transformation arise from irreversible damage to mitochondria

 Normal cell energetics: 1:18
 Cancer cell energetics: > 50% energy from glycolysis even under normoxia
 Increased glucose demand: Glut1, Glut3
 Hexokinase II, PFKB3, PKM2, LDH,
 PDHK –I PDH, pyruvate carrier down

Warburg (1925) J Cancer Res

 Glycolysis and Cancer

Crabtree Effect:

 No change or slight increase in O2 consumption in normal cells

 Decrease in O2 consumption in presence of high glucose
Also seen in hematopoietic progenitor cells, spermatozoa, intestinal mucosal
cells, renal cells, embryonic stem (ES) cells and microbes.
Biochem J (1926)
Glycolysis and Cancer
Glycolysis and Cancer