Vv yr Vv yy Vv Vv yyIntroduction & Objective Guava is a sweet, juicy and light dark green coloured fruit, when ripe it acquires a yellow colour & has a penetrating Strong scent. The fruit is rich in vitamin C& minerals. [tts a rich source of oxalate and its content in the fruit varies during different stages of ripening. In this project, we will learn to test for the presence of oxalate jons in the guava fruit and how ft amount varies di ifferent stages of ripening.the fruit by boiling te ions are estimate lution with standa Oxalate ions are extracted from pulp with dil. H2SO«. Then oxala volumetrically by titrating the di KMn0O¢ solution. Requirements: - 100 ml. Measuring flask, pestle & mortar, beaker, titrat flask, funnel, burette, weight-box, pipette, filter paper, dilute H2SO4, N/10 KMn04 solution, guava fruits at different stages of ripening.|) Weigh 50.09 of fresh guava & crush IC 10 a fine pulp using pestle-mortar. \) Transfer the crushed pulp t0 a about 50 ml. dil. HzSOsto it Bot. about 2 minutes. ) Cool & filter the contents ina 100 ml. measuring flask. Make the volume up to 100 ml. by adding distilled water. |.) Take 20 mL of the solution from the measuring flask into a titration flask& add 20 ml. of dil, H2S0« to it. Heat the mixture to about 60°C & titrate it against N/10KMn0, solution takenina burette. j.) END POINT: appearance of permanent Light- Pink colour. 5.) Repeat the exp. With 50.0 g of 1,2&3 days old guava fruit. beaker & add ] the contents for© Weight of Guava Fruit taken: 50,09 © Volume of guava extract in titration:- 20.0 ml © Normality of KMn0« Solution:- 1/10 Guava | BuretteReadings Con. Vol. of extract fram || __ Initial Final _|N/20KMn0s _ | Fresh Guava | 150 18 | __132 mt. I day Guava | 150 113 | 137 mi. |2day Guava |150 10.8 139.2 mi Concordant volume: (X) 136.06 ml. For fresh Guava, fone extrecyy iV = N2V2 Cerecsnet) Nixt0=1/10x 136.06Notnmility of Oxalate, N=(X)/100 Strength of oxalate in Fresh guava extract = Normality x Eq. mass of oxalate ion = 1.32/100 «44 = 581 g/L. of the diluted extract 1.) Strength of Oxalate in 1 Day guava extract = Normality x Eq. mass of oxalate fon = (X)/100 x 44 = 1.37/100x 44 = 0.603g9/1 of the diluted extract 2.) Strength of Oxalate in 2 Day guava extract, = Normality x Eq. mass of oxalate ion = (X)/100 x 44 = 139/100 x44 = 0.612 g/l. of the diluted extract iResult » Strength of Oxalate ion in, e Ereckh ¢ ay , esr Guava: 0.58 g/L © 7 Rev Gems 0604/4 4 Day Gueve: 260¢/1 _ © 2 Dev fame Dita q “ Fresence of oxalate ion is high in guava, about #< 22.57 % of guava contains oxalate ions, amount of axalote ion decreases as it ripens!!!BibliograP EY Lab manual. Google. Wikipedia. NCERT BOOK. VVV V