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  • Resazurin Day14

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    Tuba Kizilirmak
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    The document summarizes a fluorescence reading performed on a Greiner 96 well plate using a Tecan i-control instrument. The reading was conducted with 540nm excitation, 590nm emission wavelengths, 30 gain, 25 flashes at 20us integration time. Readings were taken from wells A1-A1 to H8-H8 and results are reported with intensities ranging from 9322 to 19804. The reading was performed at 21.5°C and took 40 seconds to complete.

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    Resazurin Assay

    Original Title

    20220712_Resazurin_day14

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    The document summarizes a fluorescence reading performed on a Greiner 96 well plate using a Tecan i-control instrument. The reading was conducted with 540nm excitation, 590nm emission wavelengths, 30 gain, 25 flashes at 20us integration time. Readings were taken from wells A1-A1 to H8-H8 and results are reported with intensities ranging from 9322 to 19804. The reading was performed at 21.5°C and took 40 seconds to complete.

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    9 views3 pages

    Resazurin Day14

    Uploaded by

    Tuba Kizilirmak
    The document summarizes a fluorescence reading performed on a Greiner 96 well plate using a Tecan i-control instrument. The reading was conducted with 540nm excitation, 590nm emission wavelengths, 30 gain, 25 flashes at 20us integration time. Readings were taken from wells A1-A1 to H8-H8 and results are reported with intensities ranging from 9322 to 19804. The reading was performed at 21.5°C and took 40 seconds to complete.

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 3

    Application: Tecan i-control Tecan i-control , 2.0.10.

    0
    Device: infinite 200Pro Serial number: 1404001413 Serial number of connected stacker:
    Firmware: V_3.37_07/12_Infinite (Jul 20 2 MAI, V_3.37_07/12_Infinite (Jul 20 2012/13.56.47)

    Date: 26/07/2022
    Time: 16:51:43

    System MECANOBIO2
    User Mecanobio2\insigneo-lab
    Plate Greiner 96 Flat Bottom Transparent Polystyrol [GRE96ft.pdfx]
    Plate-ID (Stacker)

    Label: Label1
    Mode Fluorescence Top Reading
    Excitation Wavelength 540 nm
    Emission Wavelength 590 nm
    Excitation Bandwidth 25 nm
    Emission Bandwidth 20 nm
    Gain 30 Manual
    Number of Flashes 25
    Integration Time 20 µs
    Lag Time 0 µs
    Settle Time 0 ms
    Mirror (Automatic) 50% Mirror
    Part of Plate A1-A1; A3-A3; A5-A5; A7-A7; A9-A9; B2-B2; B4-B4; B6-B6; B8-B8; C1-C1; C3-C3; C5-
    Start Time:26/07/2022 16:51:44

    Temperature: 21.5 °C
    <> 1 2 3 4 5 6 7 8 9
    A 13651 14825 9322 18302 18544
    B 14532 8511 9637 17677
    C 15615 15115 13410 19804 20009
    D 14285 12303 13356 20386
    E 14504 15292 10663 19549 19681
    F 15391 10244 10792 19344
    G 1462 1521 1583 1474 1548
    H 1539 1534 1600 1546

    End Time: 26/07/2022 16:52:24


    mber of connected stacker:

    6-B6; B8-B8; C1-C1; C3-C3; C5-C5; C7-C7; C9-C9; D2-D2; D4-D4; D6-D6; D8-D8; E1-E1; E3-E3; E5-E5; E7-E7; E9-E9; F2-F2; F4-F4; F6-F6; F8-F8
    -E9; F2-F2; F4-F4; F6-F6; F8-F8; G1-G1; G3-G3; G5-G5; G7-G7; G9-G9; H2-H2; H4-H4; H6-H6; H8-H8

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