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Oleh Verena i resto eclasi Peygien elo _dranaling Vik dari sem epek Nergeuing mayun thdet beyak sasavees ReaNt Yeh. Deeltar Divieron Vndrees (#01) Unto 5@ mua Fond absgerimental catay bg gh _untst meng hibvwy baikront eos des Hr 4 Aemgon pengecaclion teh tong Aveda Atagm tow de> neutron deboygi (3 den 4 Gy) «Dien qenoadnan 8¥WWs sel yoy Armbdeal eadind merghalogs Yeclehon outa fel bevinby end yong cvkop Chabel 1). Mestayun a-l on wena wile CUDN) yoy marig Aegan sel yong duis alo Oy de Tey , yg tedah corm youy bet nae ee PAPFRI INT {Date. wertln Teds Aayak Aad Foreman mpologapa hash Fowgabibel deren perlaon tem, Fan _yorg atorak . WD) yvbok doa eyenwen _yeegowal neslen veg geoph trl Table 1. Average number of nuclei per cell in directly exposed and bystander cells for neutron and cobalt-60 gamma rays. Radiation dose (Gy) Nuclear division index ifs a Guissiocis——=SSSS”S*~*~*~*~*« BG a ue Direct ——=sBystandet™ Cire i Be Neutrons 0 (pote a an 198 an mea on ak sa os 161 220 139 200 =p 146 206 140 138 = hs 13s 199 12s 23 2 126 ne us 193 t y aot 207 1 213 pees « ut 230 109" 2 cobst-o y iit 192 1 1 wn 4 ec ont : 197 z 1 os 17 19 150 ne ped 1 1 12 11 193 2 124 1 m1 wa Rie =f ua 1s us v2 = 5 196 199 us ss neutron data shown are for repat 1 the values fr replicate 2 were sma (p>005). — | combined values ofthe contol preadation, postradaion and taniporation conte, -Mecia without cells was kradiated with 4 Gy and wansfered to uniradated cls. Too few high qulty biwuceated cells were avaiable for scoring. ot10137¥Joumatpone 00969471001 oes at ee ee Det Vedoa gars sel, wads Wyong degen neutron hasilknn pening haben | dems = responce yory giles dolaws gueaiah mnikronvkleus, Clumber 1a) don yerbaton __pvieozlasmn Ctantior th) per 108 Se) bberinh Wun Karena Wigan Rinbrol dons © Cyady pra _rochas ) gasea thers dan Forkrel Aransgortas, Js yale mitronsbleus clan unlub jembalan ovbVoplisma secarn statsht tet berbedn mby Samm tan Sepérl yey ds ben buen _ len ANOVA, Vows welaporkem lea onral yong ai Fompulty wiht hHE abhir im we Swe hata tain ec omen a Bea Se 2 BGM15036 I a ge OGM15510 & — 88 reo) scwneoss + #2 i 2 coo ie — ¥3 a0 So ek | i 400 pee ee ] Fw 2) 3) pos 3 | iia ° LJ a | - i = bb ° os 1 15 2 o os 1 18 2 Sore (on) F i Dovey) Figure 1. Mironuce a) and nucleoplasme bridges : Nba ee arma nomen tmphabestae te —~ rectly neutrons vet Ies wereen Meet a confidence intervals. i pene Seema pone.058947 001 t~Yorengrs Oe Regan aBaaw Meroe a Ties SA | yoy chobale dengan MLM dary seh radar nivlron Wek menuyfetbon rs “yalen you (ag mpten alam prkvenay mitromubleus dr bendingken’ dengan ontrol yay da (erlokon yalsu unlit astern vlawy Aan valk. paris sel ana pon Co arbar2) it ong. drharaykon ;, Vontrol media, sign dom tonld 0 by ddat berbeda secoro shake ble 0,08)... Dale, seh MISSI, mesteyun grekoensn miveonseys memionban Varies, aobotansiad., yas Yuet ada Wehr yay bonsisten, Faken ope feganah . Sel ANISOBE 9) yong imenertera | ICM: dari dohS, aga yun garg \ebth. besan. o “manatee greorn mikroneleleus yoy wurip saby stma fain dan, Femuanyer lebih tendo dave nilar dos O | meskipun perbedaanga dak Sgnipiken secares stabishie, Frekocwen prbaton nubleoplesma Clanbar vb) b) menunhkFen writes yang kop besar dam Vidat ade respon Kerkart dons yg fenaghen ley\hat uulok Yedoe agers sal ~Unht — bedoa prs sel aan ontok tedoa hha atin gente | Jerboa mikromdlees dow nukleoglasmar a ben dungeon devon Feubrol , dat ade Fonds cer atone ry mengbabl-en gunbahewr prekuenm Y9_Sgnipiten Secara shushk wht soloh sabu ie geveneten =e — 0 curssi0 peer 1 cmis0s6 ey : 0 "4 tee a 1 18 2 3 4 Dose (Gy) react 3 1B omssi0 cease ree a Nog eee 5 cwte036 ea ag Mie ea § » i * o < Seat ee Lod q 10 | ‘h i : oe 2 r oO "a toda q 05 4 16 2 — _ i —— om Dewe(on —— Wd tyslenker Crees [rola cota enna: _Reras Tnelron yoag ch gonokion se ddan perecboon. wi tertontam nan Aergen Non jada waka) aekukar 5%, Merbiyun eet Yorganah det Acematy Acryen aise Fai pervaater onlut meranbuton a pats! polon dapa b L 4 dat Wer Poalon sy, an ete yore yecle dees yong! Aguenben, we + hy ~ Over vel ie Yo the Chabon ia eeDeere ee Yam wonbideeor 50 GMISFID’ Aan GAISO3E Aca ULI yng dk geolels day Moai a alk-Go pate dows PMs sa yore Sesion arg Velah “Aniediasi dengan Fob ae Te pale Splare 5% deri Aas neutron . Hasina, Arhmpkten pads (ganbs naerong “ohwe golon desis emda wm bidet menrosikur opel poryaral . Stooge Keb rpet | SignipticenJclain prekvencr mikronvtlevs | alov you Widele Aanori )) seperh yan Abelten éleh carols 4 An -buadrak: onbral mela 244A memiblr wer Yor worry Aegan 0 by (vewl)) eulvk edo Bie "Neutron Bystander Effect and Relative Biological Effectiveness a 0 3 OGM15510 3e Bi 88 M5036 kz 0} He i 23 0 zit) 8 © "02 "025" 005 007s 01 015 02 (med on dose (6 Ae 1 or) ao BB) acusst0 # » 2m18036 a ‘ ie th © 02 "0025" 005 0075" 01 015 02 (media ony) Dose (Gy) Figure 3. Micronuclei (a) and nucleoplasmic bridges (b) per 1000 binucleated cells in normal human lymphoblastoid cells treated with concitioned media from cells that were directly irradiated with cobalt-60 radiation at doses equivalent te om ofvie renee doses. Vertical lines represent the 95% confidence intervals, 0101371/journal pone 0098947 9003, Ronee Contre Experiment * Direct Dangp Induced by High Doses Op Cabal -to Garma — Pays . “yu z W : vag keaton \ Vouk dy MLM} dak wen oyppblecn ponng ag a a yentalon Ai bawdigkem | degen’ sel Vntok menegatean Yembel bahwa toudi atsgerimen yo dh guuaban diéiny ee _trasapy melthal fee langcung Cyoly Wake puyanal) davi pporan O10) de CMISOW cpebupa segab ~Signupeon Cy , 0, 0001) wanvsia Sed selame ber behon es‘Neutron bystander Effoct and Relative Biological Effectiveness = He) ome He) Ss = Wa Boy te | a a aeeag ocean en eae P ER eels baad Dose (Gy) 85 20) Sate cil) aeome is gt 15 5GM15036, ae 4 is neta thes fl 2 tom I | ee 0 (media 05: 2 3 4 1 Tae me Dose (Gy) Figure 5. Micronuclel (a) and nucteoplasmic bridges (b) per 1000 binucleated cells in normal human lymphoblastoid cells cultured In conditioned media from cobalt-60 y-irradiated cells. No bridges were observed inthe & Gy sample for GMIS510 cells, of forthe media only samples for either cell line. Vertical lines represent the 95% confidence intervals. ¢oi0.1371/journalpone.0096947-9005 Bushee Component op She Adal dose response op caball co gimme Fay cand Auhy dhe _wekansime ata lek orsakon faigsong dan bt dkerglban ndependen CAPES Seleler Aole\ cherhadiy road gogion alah gomlah op popsran Noxgond clon heb larg Sorg \o_Yomyontn gu spunat olan ferpons dows tebs\ tbe - Go saver gainma’ teers soe dort slater 4 tl Wmgga 36% alk mnbronblens } uabok dpinbelan , kompontn Peyaral Noor Erseur- Vwngpe 6% wnlot so] MISS Io. don Winggn T% untuk Sel CMIS036. (Taba don 3) Scballenya , hdot ao! igpicel yy) signipilean secure dtebishte Eom go nen unbol__naitron Clea hak dhamplten) ) 4[Table 3. Percent contribution of the bystander effect to the total direct exposure effect in cobalt60 irradiated cells for nucleoplasmic bridges. a cobsl-60 gamma dose (Gy) Bridges/1000 binucleated c Bridges induced” Pee eee Z ‘bystander component = Direct exposure Bystander exposure Direct exposure ~ |wis510 ets 0 120 20 00 00 : os mo 20 20 00 02 —hfro 250 20 10 00 00 ° : 20 10 10 190 a0 - bo 960 69 40 “9 sa a oe 960 00 uo 20 a : 5036 cts ‘ 0 so 20 00 00 — fos v0 40 250 20 9 —. ho sao «0 260 60 20 — }20 80 6 ao 49 160 tare po 0 68 40 “9 103 —— 40 550 80 a0 «o 140 Psember of bridges per 1000 bnucleated cls fer subtracting the battine (0 dos) values for that cel ne. percent fe nce otal drt exper respnse tht can bead othe nde baer ec —- Percent ‘component could ot be evaluated because the induced bytande fects gute. 40t101371 journal pone 0038947003, |Table 2. Percent contribution of the bystander effect to the total direct exposure effect in cobalt-60 irradiated cells for micronuclei. [Cobalt-60 gamme dose (Gy) Micronuclei/1000 binucleated cells Micronucle induced ‘th bystander componsat™ Direct exposure Bystander exposure Direct exposure Bystander exposure eee es510 ete —o 150 159 on oo os 1360 239 m0 140 ne ‘ ai 220 39 aa 160 6s —|20 3360 no a0 v0 4s ae po e280 0 ono au 46 foe Yeo seo ma 5680 ng 39 — oso cots -- jo 450 2s oo 00 Jos 1689 na ao 7 asa Perens re 2370 46 120 42 240 pr Lo 270 95 220 mu 1s —/so w30 sea a0 sao a re lo 3580 550 so sos 97 umber of mironudl per 1000 binuceaed cls ater subtracting the baeine (0 dose ales fr that cel Be — | "Percent ofthe induced total direct exposure response tat can be atibuted to the Induced bystander effect. - | 4%10:37 ownage n098947:082 Radne Bidigaes eggechveness CHEE) op Yhe OW CATS fash Mtuhrom. FBC _adaloh aco doot yor) _mereayon ogee clogs _yory sama unlut don jens rode “Wake rexyore bloge ont Fednrn ges rats sea Write erhad degen dots Sh yong berlba A cam , VDE sega _snsve efeF bwlens peel ders pong sea Drsqni nslron RE A Wwhvry relahp Lerhradel finar gammatsbale=60 denon membogt frekoencn mitronviless bow gentbobon pony ai yereleh dol Fel yay Asinany _lerypung Atzpr _neckon fade schep esis rekvendh yong A perldh Adlon yong akaran Wagoory, degen gehen a dogg2 & yong, Sawin. HOE nertron vil semoa dots Aan kedva sth gS ertalah 2,060.6 unbek watronbleor den $002.4 unht jorbaten Cabal 1), mewgpbten loahwa nevtron 2 Vinge Yermpir 6 Fal hh Yoyok cpethe clam mvsat sol 5 ini ch bowbngh-on dengan Yoball => gowwa mys. p Table 4, Relative biological effectiveness for neutrons relative to cobalt-60 gamma rays for micronuclei and nucleoplasmic Induced bridges/1000 binucleated cells ppg» Dose din Gy) {Cobaie gamma rays Netrone Cobai:60 gamma rays Neutrons ass cs o oo * oo ° hee os mo 2550 a 2» “00 200 —ho 200 ssi 10 9 st — 20 810 nes 0 v7 2 a oss cats ae om * oo ° — os v0 1730 “ 20 ne 10 ho 120 ans 2 no 120 a eae ee 220 503 2 a0 133 30 eee mesa + SE both cel nes re a sem sf mironucl x bridge per 1000 binceates cel afte subtracting the baseline (0 dot) values, which wee 15.0 and 45.0 for micronuclei and 129 and 52 fe rss fox cob gars for G¥18510 and GM15036 cells respective, For neuors the bassline values were 280 and 29. fot micronuclel ond 20 aed tis for Bisse for GMIS510 and GMISONG cell respecte. Dologialefectveness: neutons/cobak garima rays.Feswmpoln / Condy How + 3 TWdak ada Peingeatan yang Sngnpiten dalam prebutna- wikromblevs akan Yembolon ya damaly pada dows foto ww bake vodut. gansta bebike \ A tathor MEM”. Sept yng di haraptes , polon dows binggn merqundubar’ epee peypnal yang elas dy bedua gars sel _Uobt unmkronveleos dan pewbatar Data wi weevadta bolus neutron dk mergindba’ efee prgauak dow Sa -sel_wy _. eWay, nevbron mam, epee biclogs relahp 2.060 .18 wWnbge) -qackronipleusin'S <6bR:9 bulb Jebeon Abonduglean | dean! Vast om omgker welovon heya, feap rediam denen néviron cepat den Vvubt eaeliedas Godan! pangaton shaudur voloks aoe neidiay A ene) Kestlamaban radiey neutron .