Title: Antimicrobial Activity of Mexican Sunflower (Tithonia diversifolia) Leaf and Seed

Extract on Escherichia coli

Research Questions:
1. What is the antimicrobial activity of Mexican Sunflower (Tithonia diversifolia) seed
ethanol extract based on:
1.1 25% concentration
1.2 50% concentration
1.3 75% concentration
2. What is the antimicrobial activity of Mexican Sunflower (Tithonia diversifolia) leaf
ethanol extract based on:
2.1 25% concentration
2.2 50% concentration
2.3 75% concentration
3. Is there a significant difference in the antibacterial activity of the different concentrations
of Calabash seed extract and Calabash leaf extract?

Model Paper:
#1: https://hero.epa.gov/hero/index.cfm/reference/details/reference_id/1228026

#2: http://www.experimentjournal.com/sites/default/files/2019-06/25.Odeyemi%2C%20A.%20T
%20et%20al%2C%20The%20Experiment%2C%202014%2C%20Vol.%2020%284%29%2C
%201421-1426.pdf

#3: https://www.researchgate.net/profile/Abiodun-Ayandele/publication/
285076676_Antimicrobial_activity_of_aqueous_and_ethanolic_extracts_from_Tithonia_diversif
olia_and_Bryum_coronatum_collected_from_Ogbomoso/links/5aa82be6458515b024fb054a/
Antimicrobial-activity-of-aqueous-and-ethanolic-extracts-from-Tithonia-diversifolia-and-
Bryum-coronatum-collected-from-Ogbomoso.pdf

#4: https://link.springer.com/article/10.1007/s10600-020-03182-0