Chapter----Biological Safety Cabinet

Chapter allocated time:?

Chapter description
This chapter discusses the purpose, working principle, main parts and their functions, proper use and
safety procedures, preventive maintenance for biosafety cabinets.

Chapter Objective
At the end of this module participants will be able to develop basic knowledge, skill and attitude
about preventive maintenance of Biosafety Cabinet.

Enabling objective
After completion of this module the participants able to:

 Describe uses/ purpose ,working Principe, and parts of biological safety cabinet
 Differentiate ventilation equipment
 Identify classes of biological safety cabinet
 Implement proper use and safety in biological safety cabinet.
 Identify the types of Preventive maintenance for biological safety cabinet
 Perform preventive maintenance of biological safety cabinet

Chapter Outline

o Introduction
o Purpose/ use of BSC's
o Working principle of biological safety cabinet
o Ventilation equipment
o classes of Biological safety cabinets
o Main parts of Biological safety cabinets
o safety and Proper use in BSC
o Preventive Maintenance
o Summary
Introduction

Activity 1: Individual Reflection

Think and reflect what is Biological safety cabinet and its purpose?

Time=5min

A biosafety cabinet also called a biological safety cabinet or microbiological safety cabinet is an
enclosed, ventilated laboratory workspace for safely working with materials contaminated with
pathogens requiring a defined biosafety level.

Biohazard is a biological entity that is potentially hazardous to humans or their environmental through
infection. Some of the biohazards are parasites, fungi, bacteria, rickettsia, viruses and biological molecules.
Respiratory tract (lung), gastrointestinal tract skin/mucous membranes are exposed to biological agents. So,
laboratory procedures that could create airborne biohazards should always be performed in a BSC as it protects
the laboratory workers, product and the environment from aerosols or droplets that could spread bio-hazardous
material.

Purpose/ use of BSC's

The biological safety cabinet is used for the following

 To protect the worker from risks associated with the management of potentially infectious
biological material.
 To protect the product being analyzed or processed becoming cross contamination.
 To protect the environment. The cabinets are designed or used for routine work related to
pathogens ( parasites, bacteria, virus and fungus), cell culture and

Principle of Operation

Activity 2: Group activity

Discuss about the working principle of Biosafety cabinet?

Time: 10min
Biosafety cabinets (BSCs) are one type of bio containment equipment used in biological laboratories
to provide personnel, environmental, and product protection.It has an open front with inward airflow
for personnel protection, downward HEPA filtered laminar airflow over the work surface for product
protection and HEPA filtered exhausted air for environmental protection. The room air and
recirculated air are HEPA filtered before flowing downwards over the work area. The continuous
airflow is discharged to the atmosphere via the HEPA filter.

A front intake grille captures all of the air coming in through the front of the cabinet and some of the
air from the downflow filter. This split between intake air, and downflow air is what creates an air
curtain that prevents contamination from exiting the cabinet or from entering the cabinet work area.
The most critical element in containment is maintaining the balance between the intake (inflow) and
downflow air within the manufacturers specified velocity ranges.

Ventilation equipment

Activity 4: Group Activity

Discuss the difference between fume hood, laminar flow (clean

bench) and Biological Safety Cabinet?
Time =8 min

Ventilation equipment is a device that helps to maintain air quality (by filtering air particle) through
the HEPA or carbon filter as required. This equipment includes fume hood, clean bench, and
biological safety cabinets (BSCs).
 Figure: ventilation equipment chart

Fume hood
Fume hood is a type of local ventilation device and one of the simplest tools for controlling airborne
contamination. It is designed to limit exposure to hazardous or volatile toxic
chemicals, vapors or dusts to protect the user. There are two type of fume hood, ductless and ducted
fume hood. The ductless fume has a carbon filter, and the ducted fume hood has no carbon filter. The
ductless fume hood discharges filtered air to the room but the ducted fume hood discharges air
through the duct using a fan at the exhaust end pulling air out of the hood and expelling it to the
atmosphere.

Figure 1: Ductless and ducted Fume hood

Laminar Flow Cabinet (Clean bench)
Laminar flow/clean benches also called PCR workstation are devices that look similar to a biosafety
cabinet. Clean bench provides filtered air across the work surface to protect against contamination but only
protect the product from contamination. These devices should never be used for handling biological,
hazardous, toxic or sensitizing materials. Therefore, a clean bench offers product protection only, no
personnel or environmental protection.

There are two types of laminar flow:

1. horizontal laminar flow cabinet
2. Vertical laminar flow cabinet.

Figure 2: Laminar Flow Clean Bench

Figure: Difference Between horizontal and vertical Laminar Flow

Classes of Biological safety cabinet

Activity 3: Group discussion

Discuss about classes of biological safety cabinet?

Time: 7min

There are multiple types of biological safety cabinet classes. But what are they and how are they
different? Biological safety cabinet classes are categories describing how the cabinet works and what
it protects. These “categories” are Class I, Class II and Class III.

Class I BSC’s

A Class I cabinet is defined as a ventilated cabinet for personnel and environmental protection. Class
I cabinets do not offer product protection from contamination, significantly limiting their
applications. They provide an inward flow of unfiltered air, similar to a chemical fume hood, which
protects the worker and primary environment. The environment is protected by HEPA filtration of
the exhaust air before it discharged into the laboratory or to the outside via the building exhaust duct.
They may or may not be ducted outside. Class I cabinets are safe for use with agents requiring
Biosafety Level 1, 2 or 3 containment.
Class II BSC’s

A Class II cabinet is defined as a ventilated cabinet for personnel, product and environmental
protection from microbiological work or sterile pharmacy compounding. Class II BSCs are designed
with an open front with inward airflow (personnel protection), downward HEPA-filtered laminar
airflow (product protection or cross contamination) and HEPA-filtered exhaust air (primary and
secondary environmental protection). These cabinets are further differentiated by types based on
construction, airflow and exhaust systems. These types include A1, A2, B1 and B2. They require all
biologically contaminated ducts and plenums to be under negative pressure or surrounded by negative
pressure ducts and plenums. Type B2 cabinets take this a step further, requiring all biologically
contaminated ducts and plenums to be under negative pressure or surrounded by directly exhausted
negative pressure ducts and plenums. Like Class I cabinets, Class II cabinets are safe for work using
agents requiring Biosafety Level 1, 2 or 3 containment. Air is drawn around the operator into the
front grille of the cabinet, which provides personnel protection. In addition, the downward laminar
flow of HEPA-filtered air provides for product protection by minimizing the chance of cross-
contamination along the work surface of the cabinet. The air flow through exhaust HEPA filter, it is
contaminant-free (environment protection), and may be re-circulate back into the laboratory when
we are using (Type A) and ducted out through building when we are using (Type B).
Class II, Type A1 and A2

A Class II, Type A1 cabinet must maintain a minimum average inflow velocity of 75 fpm 0.38m/s
through the sash opening. They may exhaust HEPA-filtered air back into the lab, or may be exhaust
outside using a canopy connection. They are suitable for work using biological agents without
volatile toxic chemicals and volatile radionuclide's, but not for sterile hazardous pharmacy
compounding.

Figure--- Class II A1, A2 biosafety cabinets

Class II, Type A2

Class II, Type A2 cabinet must maintain a minimum average inflow velocity of 100 fpm or 0.51m/s
through the sash opening. Like Type A1 cabinets, they may exhaust HEPA-filtered air back into the
laboratory, or may be exhausted outside using a canopy connection. Type A2 cabinets with a canopy
connection are safe for work involving biological agents treated with minute quantities like hazardous
chemicals.
 Figure--- Class II A2 safety cabinets

Class II, Type B1

The main difference between Type A and Type B cabinet is: Type B cabinets must be operated
with an external blower and it exhausts air to the external environment via a dedicated
ductwork system. Without the external blower, the cabinet’s internal blower will blow the
air (and microbiological agents) inside the work zone through the front opening, towards
the operator’s face, creating a dangerous situation

Class II, Type B1 cabinet must maintain a minimum average inflow velocity of 100 fpm or 0.51m/s
through the sash opening. They have HEPA-filtered downflow air composed of mostly
uncontaminated re-circulated inflow air and exhaust most of the uncontaminated downflow air
through a dedicated duct that exhausts outside after passing through a HEPA filter.
 Figure--- Class II B1 safety cabinets

Class II, Type B2

Class II, Type B2 cabinet must maintain a minimum average inflow velocity of 100 fpm or 0.51m/s
through the sash opening. They have HEPA-filtered downflow air drawn from the lab to the outside
air (not re-circulation in the cabinet). All inflow and downflow air exhausted to the atmosphere after
filtration through the exhaust HEPA filter without recirculation in the cabinet or not returned to the
laboratory. Because of this, they are sometimes referred to as 100% exhaust or total exhaust cabinets.
Type B2 cabinets are suitable for work involving biological agents treated with hazardous chemicals
and radionuclides required as an adjunct to microbiology applications.

Figure--- Class II B2 safety cabinets

Class III BSC’s

Class III BSC’s is designed for work with level 4 biological agents and provide maximum protection.
The cabinet is gas tight with a non-opening view window, and has rubber gloves attached to ports in
the cabinet that allow for manipulation of materials in the cabinet. Air is filtered through one HEPA
filter as it enters the cabinet, and through 2 HEPA filters before it is exhausted to the outdoors. The
cabinet exhaust needs to be hard connected to an exhaust system where the fan is generally separate
from the exhaust fans of the facility ventilation system. The exhaust air must be double HEPA-filtered
or HEPA-filtered and incinerated. This type of cabinet provides the highest level of product,
environmental, and personnel protection. However, they should not be used when handling human
cell culture materials or potentially infectious materials, or as a substitute for a biological safety
cabinet in research laboratories.

Figure--- Class II B3 safety cabinets

Main Parts and function of biological safety cabinet

Activity 5: Group discussion

Discuss about the main parts of BSC and their function?

Time: 10min

Cabinet Pressure Plenums

The cabinet work area is surrounded by negative pressure and all external seals are under negative
pressure. The supply plenum only contains room air at a positive pressure.
The main part of the biological safety cabinets and its use are HEPA filter, different electrical
components, diffuser, damper and pressure gage.

HEPA/ULPA filters

HEPA filters are disposable, dry-type particulate filters. The filter material or media is typically made of
borosilicate microfibers formed into a thin sheet, in a process similar to the production of paper. This sheet is
folded, or pleated to increase its surface area. The pleats are held in place by aluminum diffusers or by beads
of glue that add rigidity to the media pack. The pack is then set into a frame, and sealed.
The HEPA filter manufacturer establishes the efficiency of the filter by challenging it with an aerosol of known
particle size. The numbers of particles that penetrate the filter are quantified, and this establishes the efficiency
of the filter. Thus, the filters used in the Cabinets are at least 99.99% efficient in removing particles 0.3 micron.

Note: The HEPA filter media is very fragile do not touch the media. If you think the media of a HEPA filter
is damaged, do not use the cabinet. The HEPA filter integrity has been tested by a certifier before using the
cabinet.

Figure Different size HEPA filter

Electrical component

The electrical components in biological safety cabinet are motor, capacitor, speed controller,
switches, airflow or pressure relays, ballasts, transformer, fuses and circuit breakers electrical out lets
and bulbs.

Motor or Blower

The motor/blower assembly pulls room air into the front of the cabinet, and re-circulates it internally.
During its recirculation, the air is split into two separate streams. One path leads through the exhaust
HEPA filter and out of the unit. The second path flows through the supply HEPA filter, which then
flows down through the work area, as shown in Figure. The motor is preprogrammed to deliver a
consistent volume of air, even as the HEPA filters load over time.
HEPA filters
Motor or Blower

Figure Motor or blower

Work Area Lighting

The work area is illuminated by two external fluorescent lamps which provide a minimum of 100 foot-
candles of light at the work surface.

Electronic Ballast

The BSC features solid-state electronic ballasts for the fluorescent and UV lights(Optional). These
ballasts increase reliability, efficiency, and service life with lower heat output.

Ground Fault Circuit Interrupter

The outlets on the cabinet are protected by a ground fault circuit interrupter (GFCI). The GFCI is
designed to protect the operator from some electrical hazards. If the GFCI detects a hazardous
condition, it will automatically cut off electricity to all the outlets. The button in the center of the
GFCI outlet, typically the left hand outlet, will pop out. To reset the GFCI, correct the cause of the
problem, and then press the reset button on the GFCI.

UV Light (Optional)

The optional ultraviolet (germicidal) lamp is permanently installed in the work area rear wall.
Safety and proper handling in Biosafety cabinet

Specification, Installation, and commissioning of BSC

Selection of a primary containment device should be based mainly on the type of protection required
(that is operator protection, environmental protection and/or product/work material protection) and
the risk that needs to be controlled. The selection of any primary containment device should therefore
be based on the outcomes of a risk assessment to identify and control the risks posed by the
procedures being performed and biological agents being handled.

Before any procurement of BSC end user should consult about the technical specification from the
technical experts working in BSC certification. The installation of the BSC is done by manufacturer
or its technical representative and the certification will be done following the installation of the BSCs
by accredited field certifiers.

Site Assessment and Proper BSC Placement

A Biosafety cabinet should be located away from traffic patterns, doors, fans, ventilation registers,
fume hoods and any other air-handling device that could disrupt its airflow patterns. All
windows in the room should be closed.

The BSC should be located at the wall furthest from and facing the entry door. If this is not
possible, the BSC should be located on the side wall perpendicular to the hinge side of the
door.
BSCs not connected to an exhaust system should have at least (12 inches) clearance from the
exhaust filter on top of the cabinet and any overhead obstructions when the cabinet is in its
final operating position, to allow for testing of the Exhaust HEPA/ULPA filter.
Figure: Location A shows the preferred location. Location B is an alternate location.
The air supply register(s) above or near the cabinet’s location should be redirected
away from the cabinet face. (Image from NSF/ANSI 49-2018 Annex E)

All BSCs should be placed in a laboratory at a location that provides a minimum of:

o 6 inches from adjacent walls or columns;

o 6 inches between two BSCs;
o 6 inches space between both sides of the cabinet and 6 inches behind the BSC to
allow for service operations;
o 40 inches of open space in front of the BSC;
o 60 inches from opposing walls, bench tops and areas of occasional traffic;
o 20 inches between BSC and bench tops along a perpendicular wall;
o 100 inches between two BSCs facing each other;
o 60 inches from behind a doorway;
o 40 inches from an adjacent doorway swing side; and
o 6 inches from an adjacent doorway hinge side.

Moving installed BSC

It is a common practice to move permanently installed BSCs to other locations within a laboratory
or to other laboratories. Despite the apparent simplicity of the job, there are certain conditions that
must be met prior to moving this equipment. BSCs should not be moved without consultation with a
biosafety professional or BSC field certifier (BSC must not move unless fumigation procedure is
done). After a BSC is moved, it should be certified according to applicable performance standards.

BSC start up procedure and preparing for work

a) Put-on lab coat and hand glove

b) Follow manufacturer’s startup procedure. If cabinet alarm condition is present, investigate root
cause before continuing. If alarm problem is not solved report to the service provider.
c) Inspect air intake grilles for obstructions and foreign materials and remove any obstructions ,
Remove all items from the work area
d) Adjust view screen to proper height

e) Turn on blower and allow at least 3-5 minutes before beginning work to allow the BSC to
“purge “particulates.
 Listen for the blower to verify there is airflow
 If installed, verify the BSC’s gauges and indicators have the appropriate readings.
WARNING: Never operate a BSC while a warning light or alarm is on unless verified by BSC field
certifiers. Call to the BSC field certifier for service failing BSCs.
f) Disinfect the interior surfaces of the BSC by wiping down with appropriate disinfectant for an
appropriate contact time. 70% alcohol is not considered an appropriate disinfectant because it
has no effect on fungal spores.
g) Place a plastic-backed pad on the work surface without covering the air intake / exhaust grills.
This will prevent spills from hitting the stainless-steel surface and creating aerosols.
h) Place all the equipment and supplies, including receptacle for waste and used pipettes, inside
the BSC to minimize entering and exiting. When placing the equipment and supplies into the
BSC:
 Segregate items that will remain clean from the ones that will become contaminated
 Place all materials as far back in the BSC as practical, toward the rear edge of the work
surface and away from the front grille and back grill of the cabinet
 Place aerosol-generating equipment (e.g., vortex mixers, tabletop centrifuges) toward
the rear of the cabinet
 Place bulky items such as biohazard bags, discard pipette trays and vacuum collection
flasks to one side of the interior of the cabinet
 A minimum number of needed items should be placed into the BSC to prevent over
loading.
i) After placing equipment inside, close the sash to the proper operating height.
j) Wait 2-3 more minutes before beginning work.
k) Move arms in and out of the work access opening perpendicular to the front of the BSC in a
slow steady motion to minimize disruption of the front air curtain.
l) Minimize penetration of the work opening air curtain.
 Work should be planned to minimize the number of times an operator's hands and arms
must enter and leave the air curtain.
 Ideally, have everything needed for your procedure placed in the BSC before starting, so
that nothing needs to pass in or out through the front air curtain until the procedure is
completed.
 Do not raise your hands inside the BSC above the top level of the sash height. If you
raise your hands above the sash height, air may flow up your hands to elbows and
possibly out of the BSC.
m) Know your "safe working area". BSC safe working area is the work tray or depressed area. All
work should be performed on or above the work tray.
Important points to be considered during Working in the BSC

 Confirm everything necessary for the procedure is already inside the BSC and is sterile.
  Ensure the position of sash at the normal operating level height
 Allow only one BSCs operator at a time.
 Sit at the BSC with arm pits level with the bottom of the sash.
 Minimize activities that create eddy currents (opening and closing doors and windows,
personnel walking near the cabinet).
 Perform all work using a limited number of slow movements, as quick movements
disrupt the air barrier.
 Disposable under pads can be placed on the work surface but must not cover the front or
rear grille openings. The use of toweling facilitates routine cleanup and reduces splatter
and aerosol generation during an overt spill.
 Hold Petri dishes and tissue culture plate lids above the open sterile surface to minimize
direct impact of downward air.
 Recap and cover bottles and tubes as soon as possible.
 Decontaminate bacteriological loops and needles

Work completion and BSC shut down

a) When finished working, remove all items slowly and away from the cabinet. Decontaminate
all waste items with an appropriate disinfectant and place into the proper waste receptacle. If
using a plastic-backed pad, remove and place in a biological waste box after other items have
been cleared.
b) Following completion of work, allow the BSC to run for 3 to 5-minute period without
personnel activity to purge air in the work area.
c) The interior surfaces of the workspace should next be disinfected with an appropriate
disinfectant for an appropriate contact time. Use of chlorine bleach in the BSC will damage
the BSC stainless steel work surface. Most surface disinfectants require a specific contact
time, depending upon the microbiological agents used within the BSC. Consult appropriate
disinfectant documents for proper application and suitability against the material used in the
BSC.
d) Use the following procedure to effectively clean or surface disinfect the BSC work zone
surfaces:
 Raise the sliding sash window to a full-open position.
 Silence the audible alarm during the cleaning process.
 Wipe all surfaces in parallel strokes from clean to dirty.

e) Turn off blowers and lights. Do not use cabinet as a depository for any lab equipment or
materials during periods of non-operation.
f) Close the sash window
g) If antineoplastic agents (medications used to treat cancer)are prepared in the BSC, it is
recommended that the BSC run 24 hours per day. This lessens the possibility that contaminants
may escape.

Safety precautions during operation

 Do not clean the interior surface of working area of BSC when the blower is on
 Keep papers, paper towels, Kim wipes, work surface diapers, vials, or any other objects
from being pulled in the back, front, or side slots or grills. These items can damage the
cabinet’s internal components like HEPA filters
 Do not store equipment or supplies in the cabinet
 Do not use the top of the cabinet for storage. The HEPA filter could be damaged and
the airflow disrupted
 Never deactivate the alarm. It indicates improper airflow and reduced performance that
may endanger sample or product
 Do not use a spray bottle to apply the disinfectant to surfaces. The solvent vapor
concentrations will be re-circulated in the hood

Figure 3: HEPA Filter blocked due to dust and paper

Preventive Maintenance

Activity 6: Group discussion

Discuss, what types of preventive maintenance you are performing in

BSC and challenges encountered during operation?

Time: 10min

General preventive maintenance required for the biological safety cabinet is for the most part
simple to perform. The routines and frequencies are shown below:

Daily
 Registering the date and the reading of pressure gage in the cabinet's logbook(ANNEX 3)
 Clean the BSC after each use. Wipe the interior surfaces with 70% ethanol
 Wipe (or disinfect) the surfaces of potentially contaminated materials.
 Decontaminate the surfaces of the BSC with an appropriate disinfectant, such as a 10%
bleach solution followed by 70% ethanol solution.
NOTE: Do not use a spray bottle to apply the disinfectant to surfaces. The solvent vapor
concentrations will be re-circulated in the hood.

Weekly

o Decontaminate the work surface and the interior surfaces of the cabinet with bleach, 70%
ethanol and distilled water as required. Clean the front glass door and the surface of the
ultraviolet lamp, using a domestic cleaning solution.
o Verify the precision of the manometer’s (pressure guage) reading, indicating any fall in
pressure flowing through the HEPA filter. Register the date and the reading in the cabinet's log
sheet.
o Do smoke pattern test according to the procedure (ANNEX2).
 Figure: Dirty underneath the working area of BSC

Monthly

 Clean the exterior surfaces, especially the front and the upper part using a piece of damp cloth
in order to remove the dust. In most of the biological safety cabinets have the exhaust HEPA
filter protective grill; if it has this grill it is possible to clean the tope surface of the grill if not
exist the grill care must be taken in order to protect the HEPA filter damage.

 Disinfect the surface of the lower compartment with 70% Ethanol or a suitable disinfecting
solution.
 Verify the state of the service valves.
 Do the tasks due on a weekly basis.

Annually
 Perform the tasks due monthly
 Check annual certification due date of BSC and report to the service provider
Performance Testing

BSC operation, as specified by NSF/ANSI Standard 49, needs to be verified at the time of installation,
moved to a new room, after the repair or replacement of parts that may impact the performance of
the equipment (e.g. filters, motor, etc.) and annually thereafter.
The purpose and acceptance level of the operational tests ensure the balance of inflow and exhaust
air, the distribution of air onto the work surface, and the integrity of the cabinet and the filters.

Caution:

The performance testes listed below must be done by certified personnel

as specified by NSF/ANSI Standard 49

The major tests performed in a BSC are:

Down flow Velocity Profile Test: This test is performed to measure the velocity of air moving
through the cabinet workspace, and is to be performed on all Class II BSCs.

Inflow Velocity Test: This test is performed to determine the calculated or directly measured
velocity through the work access opening, to verify the nominal set point average inflow velocity
and to calculate the exhaust airflow volume rate.

Airflow Smoke Patterns Test: This test is performed to determine if:

o The airflow along the entire perimeter of the work access opening is inward
o If airflow within the work area is downward with no dead spots or refluxing
o If there is no escape to the outside of the cabinet at the sides and top of the window
o If ambient air passes onto or over the work surface

HEPA Filter Leak Test: This test is performed to determine the integrity of supply and exhaust
HEPA filters, filter housing and filter mounting frames while the cabinet is operated at the
nominal set point velocities.

Site installation assessment test: these testes are performed to verify that the biosafety cabinet is
proper integrated into the facility
Summary
The biological safety cabinet is used for to protect the worker from risks associated with the
potentially infectious agents, protect the sample contamination and to protect the environment work
related to pathogens.
Biological safety cabinet can be basically classified as: Class I, Class II and Class III.
Basic components of BSC can be listed as HEPA filter, motor, cabinet pressure plenums, UV light
Fluorescent lamp, and etc.
When operating BSC, we have to make sure that we followed all the safety procedures.
Annex 1Airflow Smoke Pattern Test
The airflow smoke pattern test is performed using a smoke source (i.e., smoke tubes) in and
around the cabinet work zone and access opening to determine a visual representation of the
cabinet's performance. To perform the rest, the smoke source should be passed through the
following areas:

 A smoke source shall be passed from one end of the cabinet to the other, along the center
line of the work surface approximately 10cm above the sash height.
 Pass a smoke source along the edge of the entire perimeter of the work opening
approximately 1.5 inches ( 38mm) outside the cabinet.
The criteria used to evaluate the smoke patterns is the following
 The smoke inside the cabinet shall show smooth downward flow with no dead spots or
reflux.
 No smoke shall escape from inside the cabinet
 No smoke refluxes of the cabinet once drawn in, nor does smoke billow over the work
surface or penetrate onto it.
 No smoke shall escape from the cabinet.
Annex 2 Biological Safety cabinet check list
No BIOLOGICAL SAFETY CABINETS Checklist √= Yes Remark √= Yes Remark

X= No X=No

1 The cabinet is cleaned and disinfected

2 Check the Pressure gauge based on the manufacturer

3 Smoke pattern airflow test

 The smoke inside the cabinet shall show smooth
downward flow with no dead spots or reflux
 No smoke shall escape from inside the cabinet
 No smoke refluxes of the cabinet once drawn in, nor
does smoke billow over the work surface or penetrate
onto it

 No smoke shall escape from the cabinet.

4 Check the sash alarm if it works at the normal
opening
5 Check the UV and the florescent lamp are functional
 Annex 3 BSC logbook - daily/weekly maintenance form

Date Time Cumulative Cumulative duration Operator's Visual Sound Smoke Pressure Airflow Observations
of duration of of use of UV lamps name alarm alarm test gage m/s
use use reading (For
class II
BSC)

Change UV lamps
after XXX hours of
use (according to
manufacturer's
recommendations
Reference
 NSF/ANSI 49 – 2014 Biosafety Cabinetry: Design, Construction, Performance, and Field
Certification
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