Interpretive Standards (mm) Controt Zone Diameters (mm) Antimicrobial Agent Gomere | simcroopartans code | isk R ' 8 E.coll S.aureus | P. aureginosa reas (rosin) | grimmai) | cemcopitiy | ATCC 25922 | aTCC-25929 | “ATCC 27859 We Bioanalyse® Limited hereby declare that our AST Discs are to be evaluated according to below given clinical and QC parameters: ‘Amikacin KO | 30p8 feast “Enterobactenaceae “Staphylococcus spp. ae] sae | oi “Plesiomonas. shigelides iba spp ‘Amikacin Reo | ope ce Mees = *Enterobacenaceae, {P. aeruginosa, < . caer a] ste | 27 “Staphylococcus spp. “Amoxicii” wK2s [258 7 = “Erterobactenaceae, ‘Staphylococcus Sp. oy 2 8 gonorhoeae ‘Amoxilind clavulanic acid auc-a0_| 20110 vo 10-24 20.36 z “Entorobacteriaceae 2 | wi | 18 Staphylococcus sep <9 220 “Haemophius spp. 19 = 320 “Aminosisin™ aN | 60v0 “Enterobacteriaceae r _ 2 *Staphyiococcus SP. a6 ad “Aepiciin ai | Tug 1622 a8 = eee as | mis [ot -stephylococe sep 2 329) “Enterococcus spp 16 217 “Haemophis opp. se | teat 22 “Sireplococe (rn pneumonia) sis | 1925 | 226 Sueptococi (- _ _ : hemelytic oni) 224 ‘Ampiciindsulbactam | SAW-20 | 70/¥0 va 13 2937 = “Erierobacierioceee, “P.aeruginosa, 4 3 ee st vera | 315 and staphyiococe! “Haemophis spp. 19 220 ‘Ampictinicloxacitin | APSO | 286 [Rpramyein® apRAS | i5 va 1520 Tra 738 Zaithromyein [3506 = 228 = “Staphylococcus spp a3] war | 8 “Hamopis 9p. = = “2 “5 preumorige and r 2 sienaiee si a3 | waz] ste Neisseria meningtais = ‘Aziilin 1 7508 = = 2430 *P. aeruginosa a7 = 210 ‘etreonam Arw30 | 309 6 = Be “Enterobacteraceae | 1820 [31 “P. aeruginosa sts | 121 | 222 “Haemophive "Haemophilus sh ~ Page 1/13Interpretive Standards (mm) Control Zone Diameters (mm) Antinerotia gent | © | content 4 Disk R 1 s coll s.aureus |p. aureginoaa mo m (Resistant) | (Intermediate) | (Susceptibiey ATCC 25922, ATCC 25923 ATCC 27853, Bacitracin ™ B40 | tou = 1222 = "E cal “Pacinos co | 2 | “Saurus Carbenictin Pyi00 | 100 a8 = Tea “Entrobaconacoae 10 | m2 | 22 -B aeroinosa a3 vie 57 Cefaclor CEOs Ba aH = Enterobaceniceae nd Staphyocaceae aa | tsi | oe BS -Hemophiv 6p a6 [ iio | 320 Cefamandote wA30_ | 3009 HER 3634 = énterobacenicene and Staphylococcus ae | tsi | oe 5p Cotazoiin 230 [300 aa 7835 = “Enierooacomacaae =] 72 “Staphococcs SPP a | 1537 [Cefdinir cos | Sug 24-28 25-32 = ierobacoaceae “Staphylococcus spp. 16 1749 220 aemophivs spp _ = S a Caepime Fea | 30a a7 228 2430 “Enierovactoaceae, “praeruginsa, “1 x Rgmnaeees | 1947 18 fn stepnvococeh “Haemophilus pp. = = 226 "W. gonorooae = = = -vindars Sreptococe 3 5 . (non-S. pneumor 2 ze 224 “Streptococci inon'S pneumeniae, = <2 {Hemasieony Cefixime crm-s | Sug 23-27 = = “Enterobactenaceae 5] 118 | 210 -Haemophis sp. = = za =H. gonontoeae = = 331 Cafonicid woa0 | 300 22 2a a rierobacteracsee sa | 1617_[ 318 “Faemophis sp. i6_[ 17-19 [320 Stapylocoecs sop [15:17 [3106 Cofoperazone cers | 75¥6 ws 38 220 “Eniorbacteracese “Staphylococcus spp 215 16-20 22 Catoparazonel 7 = ces10s | 7590n0 “Enerobactoaceae panes are sa | ts19 | 220 Cefotaxime exe [30a 735 2534 ea “P. serginess “Acinetobacter and aa | 1522 | 220 Staphylococci spp “Enierbacteaceae | me | ae -Faamophivs spp. = = 226 N. meningitis = = 234 -N.gononhoeae 7 = Bat “Sirepiocoes (nen S reumonie. 8 = = 224 Hemalye ony Page 2/13Intorprative Standards (mm) Control Zone Diameters (mm) Antimicrobial Agent oxeere ‘io code | bisk R 1 s E.coll S.aureus | P. aureginosa roe reset) | onamdat | eocembn | ATCC 25922 | aTcc 25023 | “ATCC 27059 [Winans Stepiocosel] Pea) reptococc| 2s | 2627 » (ron. peamonise) E 2 Cofotaximoi ae clavulanic acid cate Cofotetan crr30 | 30g 7534 1723 = “Enterooacteriaceae, z : Staphylococcus $9. Se | = gonorhoeae. za | 2025 | 28 Gofoxtin Fox30 | 30g Be Er = "Erlerobaciaiacaae as 8 ‘Staphylococcus aureus Ey = 22 =. gonorhoeae 2s | mar |e Cetpodoxime oppo | 106 B28 28 = “Enterobacteraceae, a Clarithromycin ciRas | i5ug = 7652 = "Staphylococcus spp. as Te7 ET "Haemophilus spp. ‘0 142 213 Page 4/13‘Antimicrobial Agent “Microorganism Code Content Disk Interprotive Standards (mm) Control Zone Diameters (mm) (ovemedate) E.coll Toc 25922 S.aureus | P. aureginosa acc 25923 | AToc 27053, *S. pneumoniae and other streptococci 16 17-20 (Glindamyein DAZ 209 ‘Staphylococcus spp 15.20 *5. pneumonise and other streptococci 1618 (Colistin Gar) T00g 17 = a7 Non Entorobacteriaceae P. aeruginosa (Cotistin™ = 7a Enterobacteriacese, “P-aeruginosa, “Aeinetobacter. <15 Danottoxacin DANS ma 68 “Pasteurella multocda “Mi haemolytica Dirithromyein DrMae i806 1828 = Staphylococcus spp. 168 *S. pnoumonia Streptococcus spp. (B- hemolytic group) 1447 Dibekacin™ aK i006 “Enterococcus pp * Staphylococcus spp. (S- pneumoniae is excepted) “14 216 Doripenem 2735 *Enierobacteriaceae 20:22 Doxyeyeline *Enterobacteriacese 1143) “Acinetobacter Toa *P. aeruginosa, *Stapnylecocts 260 ‘and enterococc 1345 Enoxacin ENO 049 *Enterobacteriaceae and Staphylococcus 4 1647 SN. gonorthoeae at 3235 [Ertapenem ErPa0 109 ma Ba Staphylococcus spp. 5S 1618 Enterobacteriaceae 59 SHaemonilus sp. Enythromycin EAS 1809 2230 = “Staphylococcus SPP. and enterococe! 14.22 1620 Enrofioxacin a 159 “Enierobacteriaceae *Staphyiocaceus spp. “Ecol w22 Mannheimia haemolytica, *Pastourella multocida, *tistophilus soma 39 22 Fosfonyein F200 I 2230 E. cok and E. faecalis 1315 Framycetin™® FAi00 009 “Enterobacteraceae Staphylococcus spp <15 Flumequine” Fi30 309 *Enterobacteraceae <1 Page 5/13Antimicrobial Agent “Microorganism Content Disk Interpretive Standards (mm) Control Zone Diamaters (mm) Area) | (Suceptay E. col AToc 25922 S.aureus | P. aureginosa acc 28923 | ATCC 27853, Flucloxaeilin™ FLS “Staphylococcus 8p. Florfenicol eT *Mannteimia haemoivica, "Pasteurella multocida, *Histophilus somni N. meningitis “Haemophilus Sp. ‘Methicilin MES] Tz = *Staphylocaceus spp. 703 Mezlocilin mez7s | 7500 = 7926 “Enierobacteiacese and Acinetobacter 1820 "P. aeruginosa Minocycline miso | ‘Staphylococcus spp. *Enlerococci 1518 "Acinetobacter, *Enterobacteriacese, 1345 °N. meningitis Moxalactam WOX30_ | 30a, 7628 W5 *Slaphylocoocus 8p. Moxifioxacin Sug 2838 75 7H influenzae *H. parainfuenzae Staphylococcus sop. *S. pneumoniae ‘Mupirocin” MPS | * Staphylococcus spp. °S. saprophyticus, *S. xylose *S, cohni Aotensic resistance) ‘Nafcilin AFA | 1622 = “Staphylococcus spp. 1112 Nalidixie Acid “Enterobacteriacese 1418 219 Netimicin NET30_ | 4 BH W728 “Enterobacteraceae, “P. aeruginosa, and “Staphylococcus spp. 2 1944 215 “Acinetobacter Neomyein 3009 78 Enterobacteiaceae, *Stapnylococcus spp. 1348 217 Novobiocin®! N30 | 309) 1621 Page 7/13Interpretive Standards (im) Control Zone Diameters (nm) Amtimieroba Agent ‘Come cove : ‘n or | OR 1 s Ecol saws |i pmeepioe ses - (Resistant) | dntermediats) | (Susceptibie) ATCC 25922 ATCC 25923 ATCC 27853 Maier Heian are Stee bos fo ae | sae | on setenay ne Nitrofuranioin F300 [3009 225 62 = Ei obecatocon “Spices op aw | ase | or Eaormncis ope Ttroxonne™ WIXSO | 30h <2 [| _- | 20 [Norfioxacin NORAO | 1000 2838 17:38 2228 “Ererobaceceee, Pe esupnoss . . . Esarpeoss. se | sae | an “Enordcts ope [Ofloxacin OFXS | Sug 29-33 24-28 ATA Emerobaceacee, ‘Prsergnose ae | nas | oe “rebooted “Sagoo sa] ea | 8 “Haemohs so. = 56 PHC gonoroeas za | 5360} 87 “3. praunerise ad emer srepocnes . . |(non-S. pneumoniae, am 8 ea Stem Steandamyci | Oe [a a yeae_| 317 Satin oes 3S = = aureus Soe Siayocacea ee - . |(coagulase-negative) sf ~ 218 =a pretenie 2 (for penicillin G 7” 7 220 Seep [Oxolinic acid On| 2a. we 22 a7 = sf ae AaB ED = = cy 28°C 24-28 25-32 = ma 280 oath seat = 2 “peromonas are a | as | 20 [Oxytetracyctinet™) 0 | ane = ‘Spry ep P z ‘Enea _| a | sei | ate - srepococeus procera ae | to | os Sreptococr ep “peromones : 7 saimonicda al oad 2 Periiin P00 = 2637 3 ‘Sanyo [=] Tenia sp. Sep -N.gonerhoaae Ss —| are | ar -Stetoenes . = (non-S. pneumoniae) a9 er 2 ‘Spies op = = [a ra petri = =| Piperaitind 7007 a wesoore] 2030 7736 2530 ‘Enerbecniacese “Ereropceracen =v | 120 | oa |-stopylococas op. ab) Potomes s ioe *H_parainfluenza ~ ~ oH Piperaciflin ‘PRL100 | 100 u9 24-30 = (25-33 Page 8/13Control Zone Diameters (mm) Antimicrobial Agent Convert -ticroorganisin Code | “tisk R ' s E.colt s.aurous |p. aw rosin) | grimmma) | cncoitiy | ATCC 25922 | aToC-25920 | ‘aTCe 7863 "Erlerobaciovacaae “Acinotobacte spp av | sao | oat *P. aeruginosa a7 ~ 218 [Pefloxacin”! Pers | Sua 29-35.5 255-295 = =P. aeruginosa “Acinetabacte! spp. <6 = -Siaphylococeus sp. Pipemidi acid P20 | 205 “Enterobacioviaceae 0] ss Piimycin PR? | 2ye = 7035 = °S. aureus *Streptococcus spp_ ltd — le Poymixin 8721 P5300 | 3000 26 = = =P. aeruginosa 73 Eee ana 3 ont 212 cisi 1349 718 3p. aeruginosa ai L 22 Pras | 15g = Exar = <19 = 222 syns [45/1059 - 2128 = “Streptococcus sp. a5 | 1618 219 *S. pneumoniae Rifampin RAS | Seg 0 Er = *Slaphyoeaceus Sp. r 7 , “Enterococcus spp. a8 al al “Faemophius spp, a6 iri 320) 75 pneumonia is [1718 319 =X. meninges Interpretive Standards (mm) ‘Control Zone Diameter Limits (mm) code | Content Antitunget Agent Disk R ' s . albicans |. parapsilosis | C. tropicalis arecsoo2 | “atce'zm9” | “Arce 70 ‘Glotrimazoie ars | a s10 11-19 220 Eeonazoie' | ECOAO [10 ye as3 ss = 220 Fluconazole\" FLU25 | 25u0 28-39 22-33 26-37. Canc spp. aia iste_[ 310 Ketoconazole) Kea | 0 ne = = 320 Kreis | ie saz 2535 20 | a7 | 328 Metronidazoie® ers [Sym or anaerobic on) ‘organisms: 7 218 Miconazota"®> Mezt0 [1008 30290 a6 rs = Nystatin("°) ‘Ny-t00 | 100U 1 65-315 a = 316) Voriconazele vor’ | tg FEF 2837 Ss ‘Candida spp. =| 1416 “Amphotericin ina ee 2028 210101416 Traconazote 0 730 1928 Candida 6 <10 tora 315 Caspoungin cass | Sue ea Teas 07 Page 11/13Hints: (© FDA- approved zone size recommendations from drug manufacturers not included in CLSI_ Mi100-S13(M2-A) (According to COMITE DE L’ANTIBIOGRAMME DE LA SOCIETE FRANCAISE DE MICROBIOLOGIE (SFM) REPORT 2003 the interpretive range for chloramphenicol is valid for thiamphenicol (©!1tis given by SFM that the interpretation for Kanamycin is valid for neomycin, framycetin, aminosidin (paromemycin) (9 According to SFM the interpretation for Colitn is valid for Polymixin B. (©) Find in reference 13. Screening breakpoints are: ceftazidime (<22 mm), cefotaxime (<27 m).Qualty Control recommendations are: E.coli ATCC 25922(a5 glven an the table), K. pneumoniae ATCC 700603 ceftazicine (10-18 mm), Cefotaxime (17-25 mm) The use of more ‘than one antimicrobial agent for screening improves the sensitivity of detection. Phenotypic conformity tating equites the use of both ‘ceftazidime and cefotaxime alone and in combination with clavulanic acid, Qualty control recommendations are: negative strain E. coli ATCC 25922 which produces a ‘<2 mm increase in zone diameter for antimicrobial agent tested alone versus is zone diameter when tested in combination clavulanic act posite ‘train fork pneumoniae ATCC 700603 Which produces a >3 mm inctease in cefotaxime zone clameter and >5 mm in ceazidime zone diameter (See ‘Liitations ofthe Procedure’ for details of procedure.) {According to CLSI, Oxaeilin isthe preferred agent when a penicilinase -stable penicilin i tested and results can be applied to the other peniilin-stable peniclins,cloxacilin, dicloxaclin, luctoxacilin. (®) FDA. approved zone size recommendations from drug manufactures not included in CLS! M100.S17(M2-AS) References CONADA # 141-036, Approved by FDA Pharmacia & Upjohn Company ‘Kalamazoo, Michigan 48001, USA Revised January 2003 \) Department of Microbiology and Infectious Diseases, Veterinary Faculty University of Zagreb, Zagreb, Croatia In Vitro Antimicrobial Susceptibility of 183 Pseudomonas aeruginosa Strains Isolated from Dogs to Selected Antipseudomonal Agents. B SEOL, T. NAGLIC, J. MADIC and M. BEDEKOVIC Po: 25 (©) The Veterinary Antimicrobial Decision Support System ‘Mike Apley, DVM, PhO Department of Cinical Sciences Kansas State Universty Pgs: 11-12-19-14722, |“) Performance Standards for Antimicrobial Susceptibility Testing; Seventeenth Informational Supplement (100-817 Vol.27 No. 1 Replaces M100-S16 Vol. 26 No.) |©)BD BBL ™ SensiDisk ™ Antimicrobial Susceptibity Test Dies (©) Performance Standards for Antimicrobial Susceptibility Testing from Bacteria Isoleted from Animals; Approved Standard Third Edition (M31-A13 Volume.28 No.8 Replaces MO1-A2 Volume. 22 No.6) ©) COMITE DE L'ANTIBIOGRAMME DE LA SOCIETE FRANCAISE DE MICROBIOLOGIE REPORT 2003 (SFM) (Chaiholer : Pr C.J. SOUSSY Centre Hospitalier Universitaire Henri Mondor 94010 Créteil Cedex) '®) Recommendations for susceptibility tests on fastidious organisms and those requiring special handling ‘Anna King (Microbiology Department. GKT School of Medicine, St. Thomas’ Hospital, London SE1 7EH) (March, 2004) Po:10r10 © PRODUCT MONOGRAPH STYGACIL' Tigecycline for Injection Steril (olyeyleyetine derivative) Montreal, Canada Date of Revision August 15, 2007 ‘Submission Control Number: 114369, Pgs: 33-3463, °° ORGENICS ANTIBIOTIC DISC Disc of Absorbent paper impregnated with Antibiotic and uiized for susceptibilty testing (Kirby Bauer Method) Baver, AW., Kirby WMM. Shertis, KR. and Truck, M.(1996) Amor, J.ClinPath 45, 493-496, CLS! "Performance Standards for antimicrobial Diak Susceptbilty Tests; Approved Standard — Seventh Edition, January 2000 CLSI "Performance Standards for Antimicrobial Susceptibity Testing” Eleventh Informational Supplement, January 2001 WHO (1977) Tech Rep.Ser,No.810 FDA (1978) Codes of Fed Rebs.21 Pag.460 Pos: 2-3-4 1") MICROBIOLOGY A CENTENARY PERSPECTIVE Edited by: Woilgang K. Jokik, Lars G. iunkgdahl, Alson D. O'Brain, Alexander von Graevenitz, Charles Yanofsky lyophilized powder for intravenous use 50 mg! Vial Tetracycline Antibiotic Page 12/13,With a Foreword by Joshua Lederberg 9:42 (2) CLsI Standards 2003.41100-$13 (M2) Disk Diffusion Supplemental Tables, CLS! Wayne Pa, (°°) Comparison of Disk Susceptibility Test of Gram-Negative Rods with MIC for Cefoperazone-Sulbactam Combination, Fatima Noman, Uzma Usman, Ataf Ahmed. Deparment of Microbiology, Liaquat National Hospital, Karachi. ‘Jan-Mar 2007.5 Pos (14) Interpretive Criteria For Cofodizime In Vitro Susceptibility Tests With Neisseria gonorrhoeae Mary S. Barrett, Ronald N. Jones and Meriith E. Erwin Department of Pathology, Ant-Inectives Research Center, University of owa Collage of Medicine, Lowa City, owa USA, Received 13 Apri 1993; accepted 8 June 1993. Availabe online 14 November 2002. (15) RIN Jones, A L Barry, P C Fuchs and C Thomsbery. Disk diffusion susceptibility testing of two macrolie antimicrobial agents: revised Interpretive cnteia for erythromycin and preliminary guidelines for roxithramycin (RU 965). J Cin Microbiol 1986 August, 242): 239-238, (16) Pesro P. Chanco, Jr, M.D, MPH. (TM) and Siti Chin-On, 8 SMT. The Determination of Resistance to 11 Commonly Used Antimicrobals of Pathogenic E. col isolated from Children Age Range 1-11 in Metro Manila. Phil. Microbo! Infect Dis 1984: 12(2).74-87. Page 13/13