738 Therioganology f _IN VITRO MATURATION OF BUBALINE UM- OOCYTES INTHE BOVINE VAGINA IN MEDIUM. GN, Purohit and 8. Sharnia Department of Veterinary Obstetrics & Gynaecology, Vely Coll Bikaner, Rajasthan, | pe, Rajasthan Agric, University, In an silempt to find out simpler-cos L-effective means of in vitro fertilization, a technique of intra-vagiial culture for in vitro maturation of buffalo follicular oocytes in the bovine vagina was Weveloped. Ovaries were collected from the skiughterhouse in v plemented with 50-1U of pea and 50 Ug.steptomye Oocytes recovered by aspiration of ov: 25. mM Hepes, 0.25 mM pyruvat in 100 ul droplets under oil rm, normal saline solution sup- 2 vil withit hours of slaughter. £3 ss were cultured in'TCM 199 medium supplemented with “3 Sup/inl FSH, Sug/ml LU, Lug/il estradiol libiotics either 4 CO2 incubator for 24h or loaded in pre-sterilized emply semea straws which were scaled and then loaded in the groove of an empty commercial plastic proges- Xerone implant, CIDR-B (Controlled Internal Drug Release-Bovine, Inter-Ag Hamilton NJ). The CIDR:B was. loaded in its applicator and placed in the vagina of a cow. The irnplant was with- drawn after 24h of culture, the straws taken out and oocytes with medium emptied in-a petri dish... Oocytes were freed from the cumulus cells by vortexing and fixed with acetic methanol for 248. * Oocytes were stained with 1% aceto-oreein and examined for nuclear maturation. The proportion 4, of oocytes reaching M-Il in the laboratory culture and intra-vaginal culture was 68.88% (31/45) and } 52.70% (58/111) respectively. The proportion of oocytes not resuming meiosis and remaining at 7 "GV was 8.88% (4/45) and 25.22% (28/111), whereas, those arrested at AT-1 were 22.22% (10/45) “# “and 13.51% (15/111) in the two cultures, respectively. ‘The percentage comparisons for the two ‘$ cultures was done by Chi-square test. Preliminary results show a lower effi cultuig, however, the technique has a field application and needs experiment: ture, a 3 4 # scanned with CLamsCan