EROVULATORY RESPONS YO RECOVERY IN RATHI CATTLE SUPE! y AND EMBI R IN THE ARID ZONE. Prof. $. 5. Sharm and G, N. Purohit gue of Veterinary & Animal Science, Raj. Agri. Direcior Research (VAS), Colle nae) DIA University, Bikaner (sa INTRODUCTION fast developing embryo transfer, a egral part of the Lennon nes FSH have been exter F has the advantage of lower costs d le which form the mainstay of livelihood for, the peuple of North West of Rajasthan State, Keeping in view the econainic importing of aad genous gemplasm of high production potential , the breed needs preservation dissemination. It was therefore planned (0 attempt superovulation and embryo transfer this breed of cattle. Superovutation isan important and programmes. Although purified fr recommended the workl over, PMS lability in a country Tike India, Rathi catt ws of MATERIALS AND METHODS Superovulation was attempted in 2. groups. Group A included 2 cyclic and 2 acyclic cov ‘which were injected with 2000 LU I/M of PMSG (Folligon,Intervel,Holland) on day 8 the cycle followed by 25 mgs of Dinoprost Tromethamine( Inj. Lutalyse, Upjohn, U 48hrs. after administration of PMSG, The acyclic animals were pretreated with progestagen implant (CIDR-B,InterAG,Hamilion.NJ) containing 1.9 gms progesterone, Groy B included six cows, All the animals were acyclic and hence were treated wit aprogesterone implant followed by equally divided doses of FSH (Super-Ov, Aust Intemational USA) on day 7,8 and 9 of the implant at 12 hrly intervals. Dinoprost wa given I/M at the time of Sth injection when the implant was withdrawn. ‘At the time of superovulatory estrus artificial insemination was done at 0, 12 24 hrs of estrus using frozen semen . Superovulatroy response was assesed by cout the number of corpora lutea on day 6-7. Embryos were recovered on day 7 using standard non-surgical techniques. ES RESULTS AND DISCUSSION Superovulatory responses: The average number of ovulation in group A and B were 8. + 232 and 12.6 + 1.15 respectively. The progestagen implant had no effect on es intensity, Estrus was induced in 414 3.26 and 280 + 2.30 hrs after prostaglandin inject in group A and B respectively. = Embryo reco % in group A ry: The average embryo recovery rate, Was 75.33 + 10.35 and 71.0 + 733% nd B respectively. The number of embryos recovered s ego : : r wr of el covered per donor was go in tnt the groups, Group A yielded 8 untertilised oocytes, 6 degenerated embryos +4 stocysts and 4 morula whereas group LB yielded 39° unfertilised oocytes and 24 scanned witn Lamscana mein those showing poor (1996) have 1 Inyos per cow yes only, Animals showing anal excelle in) unterth Roxehlaw et mmber of recovered 0 FSH groups are et at va (1985) wherein results ict reason for this. cannot ct al (1978). citer when compared J with PMSG. The © 1, Ky Rowe, R. Fs po, M. Rand Ginther, 0. J (1980) Embryo transfer Gs electing supcrovulatory response, umber of transferable embryos and Theriogenology,13 (6) + 397- 406. p, HO Ns Roussel, J. D. Pool, S. Hand Godke, $. H.(1985) Studies en non surgical ‘ecavery and transfer in cattte . Korean . J. Ani. Sei, 27 (4): 208 210 | Kz Roselius, Ry Unicki,PsMichactes Unicki, P;Strehl, R; Dexne, Us Roschlan, N. (1996) Effectiveness of embryo transfer programmes. Influence of the factors, red eggs and embryos . Archiv. fur. Tierzucht 3995: 497 - 502 [bulls und recover ‘el, G. E Jr: Elsden, R.P.:Nelson, 1.D.; Beven, R.A. (1978) Superovulation of cattle pregnant mares serum gonadotropin and follicle stimulating hormone. In control of sfoduction in cow. A seminar in EEC programme of coordination of Research of Beef distin held at Galway Sept, 1977. The Hague Netherlands Martinus Nijhoff (1987): i 168 (Ani.Breed. Abstr, 48 (2): 5731 Scanned with CamScan