SOP #05.

0 1 05 July 2011

Quantitative determination of Crude Fat/Oil in Feed sample

Standard Operating Procedure SOP # 05.0

5th July 2011

Prepared by Kabir Ahmed Chohan

Approved:
CEO Vigilant veterinary service
Dr. Muhammad Azam Chohan DVM, M.Phil (Nutrition) Signature_______________________

Chemist (Vigilant Tenent Laboratories)

Miss Ayesha Sadiqa B.Sc. M.Sc. Chemistry Signature _______________________

Internee (Vigilant Tenent Laboratories)

Kabir Ahmed Chohan DVM (8th Semester) Signature _______________________

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Introduction:
Crude fat includes the entire portion of feed soluble in ether. C. Fat in most feeds may also embrace varying amounts of other ether soluble materials such as the fat soluble vitamins carotene, chlorophyll, sterols, phospholipids, waxes etc. The amount of such substances other than true fat usually represents only a very small percentage of the overall feed.

Equipment:
y y y Soxhlet Apparatus Electric balance Oven Desiccators

Reagents:
y Anhydrous Ether

Procedure:
y y Take dry filter paper weigh it as A. Make thimble of it and put approximately 1gm moisture free sample in it and put in oven for 10 minutes. Then from oven put it in desiccators for 4 minutes and weigh it as B.
nd

y y y y y y

After 2 weighing put it into soxhlet assembly and make washings with ether until it becomes clear. Make one more washing after clearance. Take out the thimble and evaporate acetone by placing it in china dish at room temperature. Put in oven for one hour at 80 C. Weigh it as C.

Large amount of water soluble components such as CH2O, urea, lactic acid, glycerol and other may interfere with fatextraction. If present extract 2gm test portion on small paper in funnel 5times with 20ml water prior to drying for ether extraction

Calculation: %age of Crude fat = B C x 100 B-A

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Precautions: y y References: y y Animal Nutrition, G.C Banerjee, Page No. 521 Official and Tentative Methods of American Oil Chemist Society The top of the thimbles should above the siphon. Thimble should be made very carefully.