35.1.2 AOAC Official Method 969.23, ‘Sodium and Potassium in Seafood Flame Photomotrc Method First Action 1969, Final Action 974 A. Apparatus and Reagents (@) Gloswar— Boosie snare and nt V0 Po Siem recteaned wth ite INO, a ise in die immediatly before se (h) Distilled water-Us0. free fom N towbar domized Use fr pre ‘ions {6) Sodium standard soins) Sock snation 1 me Nal. Dry reagent rie NaCI? hat 110°C; odio TWeigh 2512 git | L volume ska t vole wi 1,0. 2) Working sltion for flome emission 001, O08, and ‘198mg Nan Pipe. 3,and Sm Nasocksltonin sepate 19 mi volume Risks id 7 mi K sok soluion and 2 mL LINO, #0 cach fn late 9 vohine with HLO. (5) orng ‘oltons for flame obwrption 0.001, OO0D1, 1.0008, and ‘00008 me Net. Piet mL Naso slog into 100 ml ‘olumaetsk ardditeo solume with HO. Ppt 03,19, {in 50 mb sock wolton i spre 100 lace fis and dite 0 volume with HO. {) Pera sontard ios (1) Sack roltion —1 me Kim Dry anol tegen grade KCI ine) Wei 9088 gn T'Lvolmerie sk and eo slime with 1.0. (2) Bork soon for lane emission 008,007, and0 10mg Ki. Pipe 41 an 0 a sc slon ot spate 100 aL vomste fons a3 mL Natock olin each sk; dite vole vith 1D. (3) Morin selon for flame sorption 001, ‘0S, 0007, and AOI me Kl Ppt Im K stork ion ino 00m volume asad dito volumewiLO Piet, 5.7, and 10 mL dled stick solution ino npr 100 a “lume Sega lus oar REO, 8. Wet Ashing (Cawion: See Appentix 8, sey notes on dion, wet owidtion and mie asi) Prepares sample asin RUE se 38.1.0. \Weith 1 ew portion into SO mL Pyrex besker D2. hat LIU, cok and weigh it percent solids tobe Uetemned (a) Test samples witkunkwn oro high ilconten Aad al 10-ml poole ether ad warm on sear hath oF Io temperature ot plate ui ily extracted, Devan ar repeat ti test poi is deited. Proceed in (b). (0) Testsanples wth et conent AGS ml HNO, (to C1 content is desired, ld enough DIM AgNO, to presipiite chlorides [30 rt each beaker Digest on teat bth rl Temperature hot ple antl es potion dave: evaporate 0 rym AAS ma HINO, and take 0 drynew. Rest. Ad 2m HNO and warm‘ disove, Proceeds) o (6) Forflome emission Tranter ges 0 25 ml. volimerc Aas with hot water, wash down sien a eaker 3 mes wth hr wate, and ad washings to xk Cok and deo volume with 1,0 Ifpariles aetna finely anger tse cetige align 02000 pm and K: either standards and a) For flame absorption Transfer digest to. 100) ml ‘olumewie sk and proceeds above, Dilute or erect readout as Follows: Place | mi aliquotin 25 ma. volumetric ask and dite to volume with HLO for Nas place 2 ml aliquot in 10a volume flask and dilate to wohume with ILO for K Prepate bank solution by ditting 2 mL HNO: to 100-mL with Ho. ©. Dry Ashing Prepare tes sample ws in 932.07 (see 38.101), ‘Weigh gtestpomion inp crucible, andchar onelectric hotplate ‘roverlow flame. Placein cold furnace nd brn to 525°C. Ash 2h to white ash. Cool and weigh if total ash is desired Add 15 maf. dilute INO, (1-+4) ro erucible breaking up ash with string rod if necessary. Filler through ackl-washed quantitative pur int 10 mL vokimcric ask, Wash ese and paper times ‘with HO, Dilute volume, Proceed as in (a) a). (a) For lame emission. Read diel {) For flame absorption —Diue for dncet readout as follows Place Im aliquot 100/mL vol metric Mask and dilute to volume ‘with H.0 for Na place 1m aliguotin25 mL volumetric ask and dilute to volume with 1,0 for K. Prepare Blank solution by ding 2 mL HINO, so 100 mL. with Ho. D. Determination (Cunion: Seo Apganiix 8, safety nows on AAS and flame phoometers.) Follow manufacturer's directions for type of instrument available, Dilute est slutonif necessary to bring readines within range of working standards, Read Bank, standards, and test solutions at $49 nm for Na and 767 nav for K unt reproducible; record percent Tor percent sbsorption for cach, Calculations For ‘lame emission hotometersnotesuinped with dieetreadout resus are ing Noi 4 5) e-£) x woos 59] +6.) Ze portion ‘where £, = (percent T of unkown) ~ (percent T of blank) = (percent Fof standard of lower conentration than test solution) — (percent T of blank): Ey ~ (percent T of standard of higher ‘concentration than text soltion)~ (percent Tofblank}; Cm Naor Kim in standard of lower concentration than test solution; C, = mg [Naor imL instandard of higher concentration than est solution: = ilution fatr. For lame absorption photometes: Convert percent absorption to absorbance (), Plot standard curve of 4 against concenteation, Read unknown concentrations, sg Na oF K/100 2— (concentration unknown » Fx 100) test portion Reference: JAOAC 82, $5(1969), CAS-7440-09-7 (potassium) CAS-7440-23-5 (sium) 2005 AOAC INTERNATIONAL