AC FAME in AVTUR on Agilent 7890

AC FAME
in AVTUR
www.paclp.com
PAC ROTTERDAM
Kiotoweg 555
3047 BG ROTTERDAM
The Netherlands
Office: +31 (0) 10 – 4624 811

Fax: +31 (0) 10 – 4626 330
pacsales@paclp.com
service.netherlands@paclp.com
Rotterdam, December 10, 2019
Dear customer,
Congratulations on the purchase of your AC Analytical Controls FAME in AVTUR Gas

Chromatograph unit!
At PAC we develop, produce, and support high end chromatography products under the
brand AC Analytical Controls and are characterized by significant added value for your
industry. It is our goal to create instruments that meet your analytical expectations today
and in the future.
Our Quality Management system (audited against ISO 9001 standards) supports our
product development, production and service-processes to ensure that our products and
services are constantly in line with your requirements.
Before your analytical system leaves our facilities it is fully calibrated and tested to
(requested) specifications. Included you will find the final technical documentation for
your FAME in AVTUR Analyzer with the following sections:
• Analysis description
• Startup procedures
• Actual settings
• Sample preparation and sample handling
• Method description
• Qualification Results
PAC also offers you a full range of support tools to ensure the analytical integrity and
performance of your application, such as consumables and spare parts, calibration and
reference samples, classroom and on-site training, service contracts, modem support and
the AC Response Center (free helpdesk).
If you have any questions regarding our support tools or if you need assistance, please do
not hesitate to contact our Response Center:
E-mail: service.netherlands@paclp.com
Phone: +31-10-2456 248
Fax: +31-10-2456 241
Website: www.paclp.com
With kind regards,

PAC Rotterdam
AC Analytical Controls B.V.
Rick Felix
Value Stream Manager GC
www.paclp.com
AC FAME in AVTUR
on Agilent 7890
PAC Rotterdam
AC Analytical Controls BV
P.O Box 10054, 3004 AB Rotterdam
Kiotoweg 555, 3047BG Rotterdam, the Netherlands
Phone: +31-10-462 4811
Fax: +31-10-462 6330
E-mail: acbv@paclp.com
PAC L.P. (Corporate Headquarters)

AC Analytical Controls Inc
8824 Fallbrook Dr., Houston, Texas 77064, USA
Phone: +1-2815800339
Fax: +1-2815800719
E-mail: acinc@paclp.com
PAC Singapore
AC Analytical Controls Asia Pacific Ltd
61, Science Park Road #03-09/10 The Galen, Singapore Science Park III
Singapore 117525
Phone: +65 6412 0890
Fax: +65 6412 0899
E-mail: ac-asia@paclp.com
THIS DOCUMENT CONTAINS CONFIDENTIAL AND PROPRIETARY INFORMATION THAT IS THE SOLE PROPERTY OF PETROLEUM ANALYZER COMPANY, L.P. ("PAC") AND NO
OWNERSHIP OR LICENSE RIGHTS ARE GRANTED BY DELIVERY OF THIS DOCUMENT TO THE RECIPIENT. THIS DOCUMENT AND ALL INFORMATION DISCLOSED HEREIN (A)
SHALL NOT BE USED TO MANUFACTURE, CONSTRUCT OR FABRICATE THE GOODS DISCLOSED HEREIN OR ANY DERIVATIVE WORKS OF THE SAME; (B) SHALL NOT BE
EXPLOITED OR SOLD BY ANY MEANS; (C) SHALL NOT BE COPIED OR OTHERWISE REPRODUCED IN WHOLE OR IN PART; (D) SHALL NOT BE REVEALED OR DISCLOSED TO
ANYONE EXCEPT THOSE PERSONS EMPLOYED OR ENGAGED BY THE RECIPIENT AND HAVING A LEGITIMATE "NEED TO KNOW" THE INFORMATION CONTAINED IN THIS
DOCUMENT, OR IN ANY MANNER MADE PUBLIC WITHOUT THE EXPRESS WRITTEN PERMISSION OF PAC; (E) SHALL BE HELD BY THE RECIPIENT OF THIS DOCUMENT IN
STRICT CONFIDENCE AS A FIDUCIARY; AND (F) SHALL BE PROTECTED AND KEPT SEPARATE FROM THE DOCUMENTS AND MATERIALS OF THE RECIPIENT OF THIS
DOCUMENT. THIS DOCUMENT AND ALL INFORMATION CONTAINED HEREIN SHALL REMAIN THE PROPERTY OF PAC AND SHALL BE RETURNED UPON DEMAND. ALL
PROPRIETARY INFORMATION IS PROVIDED "AS IS" WITHOUT WARRANTY OF ANY KIND, WHETHER EXPRESS OR IMPLIED, INCLUDING WITHOUT LIMITATION ANY
WARRANTIES REGARDING THE TIMELINESS, ACCURACY OR COMPLETENESS OF THE PROPRIETARY INFORMATION.
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and/or typographical errors. PAC does not assume responsibility for the accuracy of any translation of these materials.
AC FAME in AVTUR Analyzer Page 3 of 52 www.paclp.com

Order #: RMA20951 P368367.3
Table of Contents
1 Instrument Description ............................................... 5
1.1 General ....................................................................................... 5
1.2 Analysis description ........................................................................ 6
1.3 Flow Diagram ................................................................................ 6
2 Specifications .......................................................... 7
2.1 Leak testing ............................................................................... 7
2.2 Hardware control test .................................................................. 7
2.3 Analytical control test .................................................................. 7
3 Startup Procedure ................................................... 10
4 Actual Settings of the Analyzer ................................... 11
4.1 Pressures and Flows ................................................................... 11
4.2 PID Settings GC ......................................................................... 11
5 Hardware Description .............................................. 12
5.1 Components............................................................................. 12
5.3 Columns.................................................................................. 13
6 Sample preparation and sample handling ...................... 14
6.1 Validation check .......................................................................... 14
7 ChemStation method description ................................ 15
7.1 CUTTING TIME IP599 C16 C18.m....................................................... 15
7.2 IP599 C16 C18.m ......................................................................... 20
7.3 CUTTING TIME C14.m .................................................................... 26
7.4 APPENDIX IP599 C14.m .................................................................. 31
8 Qualification results ................................................ 36
8.1 Cutting Time Determination C16-C18 ............................................. 36
8.2 RF check 1st dimension column ..................................................... 37
8.3 Cutting Time Determination C14 ................................................... 39
8.4 Calibration .............................................................................. 40
8.5 Validation ............................................................................... 48
9 Spares and Consumables ........................................... 52
9.1 CCG4800.100 Spare kit ............................................................... 52
9.2 CCG4800.200 Consumable kit ....................................................... 52

Order #: RMA20951 P368367.3
1 Instrument Description
1.1 General
The introduction of biodiesel into many countries has created a new threat to jet fuel
quality. It is now common for national governments to mandate the use of renewable
components in automotive fuels. One of the most common options is to blend Fatty Acid
Methyl Ester (FAME) into diesel to produce what is often called ‘biodiesel’. Biodiesel is
designated B5 when it contains 5% FAME. Pure FAME is often designated as B100.
The introduction of FAME into diesel fuel created some issues with an impact on jet fuel:
1) FAME is surface active and tends to stick to metal or glass surfaces. Where supply
chains handle both jet fuel and biodiesel this trait creates a risk of cross contamination
2) FAME is a non-hydrocarbon fuel component. The jet fuel specification states explicitly
that only hydrocarbon components or approved additives are allowed.
The international jet fuel specifications (e.g. DEF STAN 91-91) limit FAME content to less
than 5 mg/kg (ppm w/w). Levels above 5 mg/kg render the fuel off-specification.
This is a serious issue because there have been instances in which jet fuel containing
more than 5 ppm FAME has inadvertently been supplied to the airport.
A reliable and rugged means of measuring FAME content in jet fuels is required to ensure
compliance to the 5 mg/kg specification. Multi-dimensional GC using Deans switching and
refocusing technology supplies such a means.
The FAME components determined, using this multi-dimensional GC heart-cut method,
are listed in table below. These components have been specifically chosen as they
typically make up greater than 95% of the composition of the major biofuel vegetable oil
feeds currently used in biodiesel blends with conventional mineral diesel.
The IP599 method covers only C16:0, C18:0, C18:1, C18:2 and C18:3. C14:0 is included in
the appendix of the IP method.
FAME Molecular formula Symbol used
Methyl hexadecanoate (methyl palmitate) C17H34O2 C16:0
Methyl octadecanoate (methyl stearate) C19H38O2 C18:0
Methyl octadecenoate (methyl oleate) C19H36O2 C18:1
Methyl octadecadienoate (linoleate) C19H34O2 C18:2
Methyl octadecatrienoate (linolenate) C19H32O2 C18:3
Methyl heptadecanoate (methyl margarate) C19H38O2 C17:0
Methyl myrisate C15H2902 C14:0

Order #: RMA20951 P368367.3
1.2 Analysis description

A test portion of AVTUR is run neat on a multi-dimensional gas-chromatographic system.
A pre-separation takes place on a nonpolar 1st dimension column. After 1st dimension
separation the specified FAME species are transferred towards a highly polar 2nd
dimension column by doing a heart-cut. Before final separation is performed the FAME
species are trapped on the first part of the 2nd dimension column for refocusing by using
temperature-based trapping. When the last specified FAME component (C18:0) has eluted
from the pre-column the GC is set towards optimum flow and temperature setting for the
2nd dimension column. When these settings have been reached the trapping of the FAME
species is stopped to enable the FAME components to be separated from each other and
the remaining matrix on the 2nd dimension column. A flame-ionization detector (FID) is
used for identification and quantification by reference to an external standard of the
specified FAME species in a FAME-free AVTUR fuel.
The Agilent ChemStation software takes care of instrument control, data handling and
storage. Four methods are developed for this instrument.
Method Description
CUTTING TIME Determination of cutting times for C16-C18 FULL METHOD.M
IP599 C16 C18.m
IP599 C16 C18.m Determination of Fame components C16:0, C18:0, C18:1, C18:2
and C18:3 in regular AVTUR fuel samples
CUTTING TIME Determination of cutting times for C14 FULL METHOD.M
C14.m
APPENDIX IP599 Determination of Fame component C14:0 in regular AVTUR fuel
C14.m samples
1.3 Flow Diagram
DATE : File:
7890 Valve System 26/02/18 CCG4800 AC FAME in Avtur.vsd
AC FAME in AVTUR Rev. 1.0
PCM C-1
(H2)
2 Air
V1 3
1
Front
1 V2 Column 2 detector
2
FID
Refocus trap
S/SL Column 1
Back Inlet (H2)
Back
detector
monitor
FID
Column 1 = 10.73.154 1st Dimension Column FAME in AVTUR

Column 2 = 10.73.138 2nd Dimension Column FAME in AVTUR
Monitor = 10.52.029 Monitor Column FAME in AVTUR
THIS DOCUMENT CONTAINS CONFIDENTIAL AND PROPRIETARY INFORMATION THAT IS THE SOLE PROPERTY OF PETROLEUM ANALYZER COMPANY, L.P. ("PAC") AND NO OWNERSHIP OR LICENSE RIGHTS ARE GRANTED BY
DELIVERY OF THIS DOCUMENT TO THE RECIPIENT. THIS DOCUMENT AND ALL INFORMATION DISCLOSED HEREIN (A) SHALL NOT BE USED TO MANUFACTURE, CONSTRUCT OR FABRICATE THE GOODS DISCLOSED HEREIN OR ANY
DERIVATIVE WORKS OF THE SAME; (B) SHALL NOT BE EXPLOITED OR SOLD BY ANY MEANS; (C) SHALL NOT BE COPIED OR OTHERWISE REPRODUCED IN WHOLE OR IN PART; (D) SHALL NOT BE REVEALED OR DISCLOSED TO
ANYONE EXCEPT THOSE PERSONS EMPLOYED OR ENGAGED BY THE RECIPIENT AND HAVING A LEGITIMATE "NEED TO KNOW" THE INFORMATION CONTAINED IN THIS DOCUMENT, OR IN ANY MANNER MADE PUBLIC WITHOUT THE
EXPRESS WRITTEN PERMISSION OF PAC; (E) SHALL BE HELD BY THE RECIPIENT OF THIS DOCUMENT IN STRICT CONFIDENCE AS A FIDUCIARY; AND (F) SHALL BE PROTECTED AND KEPT SEPARATE FROM THE DOCUMENTS AND
MATERIALS OF THE RECIPIENT OF THIS DOCUMENT. THIS DOCUMENT AND ALL INFORMATION CONTAINED HEREIN SHALL REMAIN THE PROPERTY OF PAC AND SHALL BE RETURNED UPON DEMAND . ALL PROPRIETARY
INFORMATION IS PROVIDED "AS IS" WITHOUT WARRANTY OF ANY KIND, WHETHER EXPRESS OR IMPLIED, INCLUDING WITHOUT LIMITATION ANY WARRANTIES REGARDING THE TIMELINESS, ACCURACY OR COMPLETENESS OF THE
PROPRIETARY INFORMATION.

Order #: RMA20951 P368367.3
2 Specifications
2.1 Leak testing

Before the system leaves the AC Analytical Controls B.V. facility, the system is checked
for leaks. The complete system is pressurized both in on and off position of the valves,
including the sample loops present. The system will not exceed a pressure drop of more
than 10 kPa in 10 minutes.
Status of the system: Passed the leak test
2.2 Hardware control test
Before the system leaves the AC Analytical Controls B.V. facility, the system is checked
for functioning of all hardware present.
Status of the system: Passed the hardware control test
2.3 Analytical control test
This AC analyzer is fully checked on its analytical capabilities.
For further details, see the test results.
Status of the system: Passed the analytical control test.
2.3.1 Samples used
AC box number AC sample part number Description
20001.566 00.02.614 Cal Mix 3A 5 ppm
20001.567 00.02.615 Cal Mix 2A 100 ppm
20001.568 00.02.616 Reference 5 ppm
20001.569 00.02.617 Reference 100 ppm
2.3.2 Retention Time Repeatability

1st
C14 method
Dimension 2nd Dimension RT (min)
2nd dimension
RT (min)
Run # C17:0 C16:0 C18:0 C18:1 C18:2 C18:3 C14:0
1 11.026 26.849 31.146 32.439 34.919 38.240 41.254
2 11.027 26.849 31.145 32.437 34.917 38.241 41.258
3 11.025 26.848 31.145 32.436 34.915 38.235 41.257
4 11.025 26.846 31.143 32.434 34.914 38.235 41.261
5 11.027 26.849 31.145 32.436 34.916 38.236 41.257
6 11.026 26.848 31.144 32.433 34.914 38.233 41.259
7 11.026 26.846 31.14 32.431 34.912 38.230 41.261
8 11.025 26.846 31.141 32.432 34.912 38.299 41.261
9 11.025 26.845 31.140 32.430 34.911 38.231 41.262
10 11.024 26.845 31.139 32.431 34.909 38.229 41.262
Average 11.03 26.85 31.14 32.43 34.91 38.24 41.26
SD 0.00 0.00 0.00 0.00 0.00 0.02 0.00
RSD 0.01 0.01 0.01 0.01 0.01 0.05 0.01
Repeatability numbers were determined running sample 00.02.614 3A with method C16-
C18 full method.m
Order #: RMA20951 P368367.3
2.3.3 Area Repeatability

Run with C16-C18 full method.m
Total FAME (mg/kg)
Run # 00.02.614
1 26.28
2 26.87
3 27.11
4 27.31
5 26.93
6 27.16
7 26.86
8 28.15
9 27.59
10 27.29
Average 27.15
SD 0.49
RSD 1.82
2.3.4 LOQ
The limit of quantification for the individual FAME components is set at 1.5 mg/kg and
was determined by injecting standard 00.02.614 and performing calculation as follows:
LOQ = (10 * c * N * W * 60) / A
LOQ = Limit of Quantification (mg/kg)

c = Concentration of component of interest (mg/kg)
N = Noise (peak to peak) (pA)
A = Area of peak of interest (pA * s)
W = Width of peak at half height (minutes)
2 nd Dimension
Component Noise (pA) Area (pA*s) Conc. (mg/kg) Peak width (min) LOQ (mg/kg)
C16:0 0.029 12.73 5.10 0.051 0.3
C18:0 0.029 15.51 4.90 0.077 0.4
C18:1 0.024 12.30 4.91 0.084 0.5
C18:2 0.032 9.10 4.97 0.093 1.0
C18:3 0.020 8.52 5.30 0.104 0.8
C14:0 0.028 26.48 5.12 0.084 0.3
C17:0 0.052 12.57 4.96 0.105 1.3

Order #: RMA20951 P368367.3
2.3.5 Peak Symmetry
The peak symmetry was checked by running sample 00.02.615 with C16-C18 full
method.m
1st Dimension Peak Symmetry

Compound C16:0 C18:0 C14:0
Peak symmetry 0.97 0.99 0.94
2nd Dimension Peak Symmetry

Compound C16:0 C18:0 C18:1 C18:2 C18:3 C14:0
Peak symmetry 1.38 1.83 1.39 1.27 1.23 1.71
2.3.6 Validation results

Run with C16-C18 full method.m
Sample Target Value (mg/kg) Found Value (mg/kg)
AC Ref 00.02.616 5.3 ± 0.8 8.0
AC Ref 00.02.617 99.5 ± 6.2 98.2

Order #: RMA20951 P368367.3
3 Startup Procedure
1. Check if all boxes are delivered and unpack the boxes (see installation checklist for
more details).
2. Put the Analyzer on the bench and connect the gases to the connections on the back
side of the GC as the figure below.
3. The capillary columns are already connected during factory testing. Please check if
all the connections are still ok.
4. Setup the PC on the bench.
a) If a PC wasn’t ordered with the analyzer, install and configure the ChemStation
software on the customer PC.
b) Copy the Methods from the AC Methods and Data USB key to the ChemStation
Methods directory.
5. Connect the GC to the computer using the LAN cable.
6. Connect the power cord to the GC and switch on the GC.
7. Adjust the proper pressures, flows and temperatures using the actual settings of the
analyzer, see chapter “Actual settings of the analyzer” and “ChemStation method
description”.
8. Enter the correct IP-addresses for the PC, GC and ChemStation.
9. Start the ChemStation program on the computer.
10. Load the desired method.
11. Make sure the solvent-position and waste position in the sample-tray are occupied.
The solvent vial needs to be filled with cyclohexane.
12. The GC is now ready for analysis.
2 3
Component Gas
1 Connections Front detector; Back detector; Back Inlet; Hydrogen
EPC Restriction
2 Connection Front detector; Back detector Air
3 Connection Front detector; Back detector Nitrogen

Order #: RMA20951 P368367.3
4 Actual Settings of the Analyzer

AC Order number: P368367.3
Agilent Serial number: CN16143087
Production Date: December 10, 2019
Tested with ChemStation: C.01.10 [201]
Tested by: M. de Haan
4.1 Pressures and Flows

DESCRIPTION ACTUAL SETTING
Pressures [kPa] Back Inlet 410

PCM C 360
Flows Back Inlet Split Flow 39.3

[ml/min] Back Inlet Split Ratio 20:1
Back Inlet Septum Purge 5.6
Analysis Column (Front FID) 4.1

Monitor Column (Back FID) 4.0
Front FID Fuel Gas 29.6

Front FID Oxidizer 392
Front FID Make up Gas 39
Back FID Fuel Gas 29

Back FID Oxidizer 388
Back FID Make up Gas 39.5
Temperature setpoints Oven 230

[°C] Back Inlet 300
Front Detector 300
Back Detector 300
Signal pA (20Hz) Front Detector 4.9

Back Detector 3.5
NOTE
The flows are measured with an electronic measuring device. No precision statement can be
retrieved from these data. For analyzer settings, see chapter: ChemStation method descriptions.
4.2 PID Settings GC

Settings S/SL
Configured Split/Splitless
Ignore Ready FALSE
Cryo Not Installed

Order #: RMA20951 P368367.3
5 Hardware Description
5.1 Components
Component AC Part No. Description

1 Back inlet G3452A Split/splitless inlet with EPC
5183-4647 S/SL liner
21040.105 AC septum
2 Front detector G3462A Flame Ionization Detector
G1531-80560 FID jet
3 Back detector G3462A Flame Ionization Detector
G1531-80560 FID jet

Order #: RMA20951 P368367.3
5.3 Columns
2 3
1
4 5
Column positions inside GC oven
Component AC Part No. Description Max. Temp. (°C)

1 Column 1 10.73.154 PDS , 30mx250µmx1µm 320
2 Column 2 10.73.138 SP, 60mx250µmx0.2µm 275
3 Monitor 10.52.029 Deactivated fused silica, 300
1.8m x 0.1mm
4 Deans Switch 30.30.764 Compact Deans Switch -
5 Refocus trap 30.30.745 Mounting Bracket, Refocus trap -

Order #: RMA20951 P368367.3
6 Sample preparation and sample handling

In this application sample introduction is performed by an automated liquid sampler
(ALS). The next procedure describes the step-by-step sample introduction done by an
ALS.
• Take the sample out of cold storage and put it at room temperature.
• Allow the sample to reach ambient temperature and shake well.
• Fill out the sample in a 1.5ml GC vial and cap the vial.
• Put the vial in the auto sampler turret or the tray.
• Start up the ChemStation program.
• Create a sequence with the sequence menu.
• Append a line and enter vial number; sample name; sample type and injections per
vial.
• Save the sequence and start the sequence with Run Control\ Run sequence.
6.1 Validation check

Response calibration in all used methods is done by running standard 00.02.614. For
determination of the heart-cut times standard 00.02.615 is used.
Method Cutting Time Sample Calibration Sample Validation Sample

C16-C18 00.02.615 00.02.614 00.02.616/ 00.02.617
C14 00.02.615 00.02.614 TBD
NOTE - All Cutting time methods are tuned with the high range sample to make sure nothing is
cut off from the components of interest.
When either 00.02.616 or 00.02.617 falls outside its reproducibility boundaries the system
may either need to be calibrated again or it needs to be serviced.
If after calibration the found response factors for the different peaks are running too far
apart (by more than 25%), this is probably caused by one of the following reasons:
• The glass liner in the inlet is getting too active and needs replacement
• The 2nd dimension column is getting too active and needs replacement

Order #: RMA20951 P368367.3
7 ChemStation method description

7.1 CUTTING TIME IP599 C16 C18.m
Method Information
Method: C:\Chem32\2\Methods\IP599 C16 C18 Cutting Time.M

Modified: 12/10/2019 at 3:11:40 PM
Run Time Checklist
Pre-Run Cmd/Macro: off
Data Acquisition: on
Standard Data Analysis: on
Modify Custom Fields: off
Customized Data Analysis: off
Save GLP Data: off
Post-Run Cmd/Macro: off
Save Method with Data: off
Injection Source and Location
Injection Source: GC Injector
Injection Location: Back

Order #: RMA20951 P368367.3
=====================================================================
Agilent 7890B
=====================================================================
GC
GC Summary
Run Time 23.4 min
Post Run Time 0 min
Oven
Equilibration Time 3 min
Max Temperature 275 °C
Maximum Temperature Override Disabled
Slow Fan Disabled
Temperature
Setpoint On
(Initial) 230 °C
Hold Time 15 min
Post Run 100 °C
Program
#1 Rate 25 °C/min
#1 Value 275 °C
#1 Hold Time 6.6 min
ALS
Back Injector
Syringe Size 5 μL
Injection Volume 2.5 μL
Solvent A Washes (PreInj) 0
Solvent A Washes (PostInj) 4
Solvent A Volume 4 μL
Solvent B Washes (PreInj) 0
Solvent B Washes (PostInj) 0
Solvent B Volume 4 μL
Sample Washes 4
Sample Wash Volume 4 μL
Sample Pumps 6
Dwell Time (PreInj) 0 min
Dwell Time (PostInj) 0 min
Solvent Wash Draw Speed 150 μL/min
Solvent Wash Dispense Speed 1500 μL/min
Sample Wash Draw Speed 150 μL/min
Sample Wash Dispense Speed 1500 μL/min
Injection Dispense Speed 3000 μL/min
Viscosity Delay 0 sec
Sample Depth Disabled
Injection Type Standard
L1 Airgap 0.1 μL
Solvent Wash Mode A, B
Sample Overlap
Mode Sample overlap is not enabled
Back SS Inlet H2
Mode Split
Heater On 300 °C
Pressure On 410 kPa
Total Flow On 48.958 mL/min
Septum Purge Flow On 5 mL/min
Gas Saver On 20 After 2 min mL/min
Split Ratio 20 :1
Split Flow 41.865 mL/min
Liner A Liner has not been selected.
Column
Column Outlet Pressure 0 kPa
Column #1
Column Information PAC 10.73.154
1st Dimension Column FAME in A
Temperature Range -60 °C—275 °C (275 °C)
Dimensions 30 m x 250 μm x 1 μm
In Back SS Inlet H2
Out PCM C

Order #: RMA20951 P368367.3
(Initial) 230 °C
Pressure 410 kPa
Flow 2.0933 mL/min
Average Velocity 25.372 cm/sec
Holdup Time 1.9707 min
Control Mode Ramped Pressure
Pressure
Setpoint On
(Initial) 410 kPa
Hold Time 20 min
Post Run 410 kPa
Program
#1 Rate 100 kPa/min
#1 Value 50 kPa
#1 Hold Time 0 min
Column #2
2nd Dimension Column FAME in A
Dimensions 60 m x 250 μm x 0.2 μm
In PCM C H2
Out Front Detector FID
(Initial) 230 °C
Pressure 360 kPa
Flow 4.4728 mL/min
Control Mode Constant Pressure
Pressure
Setpoint On
(Initial) 360 kPa
Post Run 360 kPa
Column #3
Monitor Column FAME in AVTUR
Dimensions 1.8 m x 100 μm x 0 μm
Column lock Unlocked
In PCM C H2
Out Back Detector FID
(Initial) 230 °C
Pressure 360 kPa
Flow 3.8413 mL/min
Pressure
Setpoint On
(Initial) 360 kPa
Post Run 360 kPa
Front Detector FID

Makeup N2
Heater On 300 °C
H2 Flow On 30 mL/min
Air Flow On 400 mL/min
Makeup Flow On 40 mL/min
Carrier Gas Flow Correction Constant Makeup and Fuel Flow
Flame On
Back Detector FID

Makeup N2
Heater On 300 °C
Flame On

Order #: RMA20951 P368367.3
Valve 1
Switching Valve Off
Valve 2
Switching Valve Off
PCM C
PCM C H2
PCM C H2 Supplies Column 2
Aux PCM C N2
***Excluded from Affecting GC's Readiness
State***
Pressure
Setpoint Off
(Initial) 0 kPa
Post Run 0 kPa
Signals
Signal #1: Front Signal
Description Front Signal
Details Front Signal (FID)
Save On
Data Rate 20 Hz
Zero Signal Zero @ 0 min
Signal #2: Back Signal

Description Back Signal
Details Back Signal (FID)
Save On
Data Rate 20 Hz
Zero Signal Zero @ 0 min
Signal #3:
Description None
Signal #4:
Description None

Order #: RMA20951 P368367.3
The Data Analysis Parameters of the used Method are :
========================================================================
Integration Events
========================================================================
------------------------------------------------------------------------
Non signal specific Integration Events
------------------------------------------------------------------------
Event Value
|--------------------------------------------------------|-------------|
Tangent Skim Mode Standard
Tail Peak Skim Height Ratio 0.000
Front Peak Skim Height Ratio 0.000
Skim Valley Ratio 20.000
Baseline Correction Classical
Peak to Valley Ratio 500.000
------------------------------------------------------------------------
Default Integration Event Table "Event"
------------------------------------------------------------------------
Event Value Time

|------------------------------------------------|-------------|-------|
Initial Slope Sensitivity 1.000 Initial
Initial Peak Width 0.040 Initial
Initial Area Reject 1.000 Initial
Initial Height Reject 1.700 Initial
Initial Shoulders OFF Initial
Initial Area Percent Reject 0.000 Initial
------------------------------------------------------------------------
Detector Default Integration Event Table "Event_FID"
------------------------------------------------------------------------
Event Value Time

|------------------------------------------------|-------------|-------|
Integration OFF 0.010
Integration ON 10.000
------------------------------------------------------------------------
Signal Specific Integration Event Table "Event_FID2B"
------------------------------------------------------------------------
Event Value Time

|------------------------------------------------|-------------|-------|
Apply Method's Manual Integration Events: No

Order #: RMA20951 P368367.3
7.2 IP599 C16 C18.m
Method Information
Method: C:\Chem32\2\Methods\IP599 C16 C18.M

Modified: 12/10/2019 at 3:11:55 PM
Run Time Checklist
Save GLP Data: off

Order #: RMA20951 P368367.3
=====================================================================
Agilent 7890B
=====================================================================
GC
GC Summary
Run Time 50 min
Post Run Time 0 min
Oven
Slow Fan Disabled
Temperature
Setpoint On
(Initial) 230 °C
Hold Time 15 min
Post Run 230 °C
Program
#1 Rate 25 °C/min
#1 Value 140 °C
#2 Rate 1 °C/min
#2 Value 160 °C
#2 Hold Time 0 min
#3 Rate 25 °C/min
#3 Value 275 °C
ALS
Back Injector
Syringe Size 5 μL
Sample Washes 4
Sample Pumps 6
L1 Airgap 0.1 μL
Sample Overlap
Back SS Inlet H2
Mode Split
Heater On 300 °C
Pressure On 410 kPa
Split Ratio 20 :1
Column
Column #1

Order #: RMA20951 P368367.3

Temperature Range 0 °C—275 °C (275 °C)
In Back SS Inlet H2
Out PCM C
(Initial) 230 °C
Pressure 410 kPa
Flow 2.0933 mL/min
Pressure
Setpoint On
(Initial) 410 kPa
Hold Time 15 min
Post Run 410 kPa
Program
#1 Rate 50 kPa/min
#1 Value 50 kPa
#1 Hold Time 0 min
Column #2
In PCM C H2
(Initial) 230 °C
Pressure 360 kPa
Flow 4.4728 mL/min
Pressure
Setpoint On
(Initial) 360 kPa
Post Run 350 kPa
Column #3
In PCM C H2
(Initial) 230 °C
Pressure 360 kPa
Flow 3.8413 mL/min
Pressure
Setpoint On
(Initial) 360 kPa
Post Run 350 kPa
Front Detector FID

Makeup N2
Heater On 300 °C
Flame On
Back Detector FID

Order #: RMA20951 P368367.3
Makeup N2
Heater On 300 °C
Flame On
Valve 1
Switching Valve Off
Valve 2
Switching Valve Off
PCM C
PCM C H2
Aux PCM C N2
State***
Pressure
Setpoint Off
(Initial) 0 kPa
Post Run 0 kPa
Signals
Save On
Data Rate 20 Hz

Save On
Data Rate 20 Hz
Signal #3:
Description None
Signal #4:
Description None
Run Time Events

Run Time Events
#1 Time 5 min
#1 Event Valve
#1 Position Valve 2
#1 Setpoint On
#2 Time 8.45 min
#2 Event Valve
#2 Position Valve 1
#2 Setpoint On
#3 Time 8.7 min
#3 Event Valve
#3 Position Valve 1
#3 Setpoint Off
#4 Time 13 min
#4 Event Valve
#4 Position Valve 1
#4 Setpoint On
#5 Time 14.6 min
#5 Event Valve
#5 Position Valve 1
#5 Setpoint Off
#6 Time 20 min
#6 Event Valve
#6 Position Valve 2
#6 Setpoint Off

Order #: RMA20951 P368367.3
========================================================================
Integration Events
========================================================================
------------------------------------------------------------------------
------------------------------------------------------------------------
Event Value
|--------------------------------------------------------|-------------|
------------------------------------------------------------------------
------------------------------------------------------------------------
Event Value Time

|------------------------------------------------|-------------|-------|
------------------------------------------------------------------------
------------------------------------------------------------------------
Event Value Time

|------------------------------------------------|-------------|-------|
------------------------------------------------------------------------
------------------------------------------------------------------------
Event Value Time

|------------------------------------------------|-------------|-------|
------------------------------------------------------------------------
Signal Specific Integration Event Table "Event_FID1A"
------------------------------------------------------------------------
Event Value Time

|------------------------------------------------|-------------|-------|

Order #: RMA20951 P368367.3

Slope Sensitivity 3.000 25.000
Fixed Peak Width 0.030 25.000

Order #: RMA20951 P368367.3
7.3 CUTTING TIME C14.m
Method Information
Method: C:\Chem32\2\Methods\IP599 C14 Cutting Time.M

Modified: 12/10/2019 at 3:11:27 PM
Run Time Checklist
Save GLP Data: off

Order #: RMA20951 P368367.3
=====================================================================
Agilent 7890B
=====================================================================
GC
GC Summary
Run Time 40 min
Post Run Time 0 min
Oven
Slow Fan Disabled
Temperature
Setpoint On
(Initial) 165 °C
Hold Time 30 min
Post Run 100 °C
Program
#1 Rate 25 °C/min
#1 Value 275 °C
ALS
Back Injector
Syringe Size 5 μL
Sample Washes 4
Sample Pumps 6
L1 Airgap 0.1 μL
Sample Overlap
Back SS Inlet H2
Mode Split
Heater On 300 °C
Pressure On 410 kPa
Split Ratio 10 :1
Column
Column #1
In Back SS Inlet H2

Order #: RMA20951 P368367.3
Out PCM C
(Initial) 165 °C
Pressure 410 kPa
Flow 2.6416 mL/min
Pressure
Setpoint On
(Initial) 410 kPa
Hold Time 30 min
Post Run 410 kPa
Program
#1 Rate 50 kPa/min
#1 Value 50 kPa
#1 Hold Time 0 min
Column #2
In PCM C H2
(Initial) 165 °C
Pressure 360 kPa
Flow 5.6445 mL/min
Pressure
Setpoint On
(Initial) 360 kPa
Post Run 360 kPa
Column #3
In PCM C H2
(Initial) 165 °C
Pressure 360 kPa
Flow 4.8476 mL/min
Pressure
Setpoint On
(Initial) 360 kPa
Post Run 360 kPa
Front Detector FID

Makeup N2
Heater On 300 °C
Flame On
Back Detector FID

Makeup N2
Heater On 300 °C

Order #: RMA20951 P368367.3
Flame On
Valve 1
Switching Valve Off
Valve 2
Switching Valve Off
PCM C
PCM C H2
Aux PCM C N2
State***
Pressure
Setpoint Off
(Initial) 68.948 kPa
Post Run 0 kPa
Signals
Save On
Data Rate 20 Hz

Save On
Data Rate 20 Hz
Signal #3:
Description None
Signal #4:
Description None

Order #: RMA20951 P368367.3
========================================================================
Integration Events
========================================================================
------------------------------------------------------------------------
------------------------------------------------------------------------
Event Value
|--------------------------------------------------------|-------------|
------------------------------------------------------------------------
------------------------------------------------------------------------
Event Value Time
|------------------------------------------------|-------------|-------|
------------------------------------------------------------------------
------------------------------------------------------------------------
Event Value Time
|------------------------------------------------|-------------|-------|
------------------------------------------------------------------------
------------------------------------------------------------------------
Event Value Time
|------------------------------------------------|-------------|-------|
------------------------------------------------------------------------
------------------------------------------------------------------------
Event Value Time
|------------------------------------------------|-------------|-------|

Order #: RMA20951 P368367.3
7.4 APPENDIX IP599 C14.m
Method Information
Method: C:\Chem32\2\Methods\IP599 C14.M

Modified: 12/10/2019 at 3:11:35 PM
Run Time Checklist
Save GLP Data: off

Order #: RMA20951 P368367.3
=====================================================================
Agilent 7890B
=====================================================================
GC
GC Summary
Run Time 54 min
Post Run Time 0 min
Oven
Slow Fan Disabled
Temperature
Setpoint On
(Initial) 165 °C
Hold Time 30 min
Post Run 100 °C
Program
#1 Rate 25 °C/min
#1 Value 120 °C
#2 Rate 25 °C/min
#2 Value 275 °C
ALS
Back Injector
Syringe Size 5 μL
Sample Washes 4
Sample Pumps 6
L1 Airgap 0.1 μL
Sample Overlap
Back SS Inlet H2
Mode Split
Heater On 300 °C
Pressure On 410 kPa
Split Ratio 10 :1
Column
Column #1

Order #: RMA20951 P368367.3
In Back SS Inlet H2
Out PCM C
(Initial) 165 °C
Pressure 410 kPa
Flow 2.6416 mL/min
Pressure
Setpoint On
(Initial) 410 kPa
Hold Time 30 min
Post Run 410 kPa
Program
#1 Rate 50 kPa/min
#1 Value 50 kPa
#1 Hold Time 0 min
Column #2
In PCM C H2
(Initial) 165 °C
Pressure 360 kPa
Flow 5.6445 mL/min
Pressure
Setpoint On
(Initial) 360 kPa
Post Run 360 kPa
Column #3
In PCM C H2
(Initial) 165 °C
Pressure 360 kPa
Flow 4.8476 mL/min
Pressure
Setpoint On
(Initial) 360 kPa
Post Run 360 kPa
Front Detector FID

Makeup N2
Heater On 300 °C
Flame On
Back Detector FID

Makeup N2
Heater On 300 °C

Order #: RMA20951 P368367.3

Flame On
Valve 1
Switching Valve Off
Valve 2
Switching Valve Off
PCM C
PCM C H2
Aux PCM C N2
State***
Pressure
Setpoint Off
(Initial) 0 kPa
Post Run 0 kPa
Signals
Save On
Data Rate 20 Hz

Save On
Data Rate 20 Hz
Signal #3:
Description None
Signal #4:
Description None
Run Time Events

Run Time Events
#1 Time 26 min
#1 Event Valve
#1 Position Valve 2
#1 Setpoint On
#2 Time 28.45 min
#2 Event Valve
#2 Position Valve 1
#2 Setpoint On
#3 Time 29.5 min
#3 Event Valve
#3 Position Valve 1
#3 Setpoint Off
#4 Time 32 min
#4 Event Valve
#4 Position Valve 2
#4 Setpoint Off

Order #: RMA20951 P368367.3

========================================================================
Integration Events
========================================================================
------------------------------------------------------------------------
------------------------------------------------------------------------
Event Value
|--------------------------------------------------------|-------------|
------------------------------------------------------------------------
------------------------------------------------------------------------
Event Value Time
|------------------------------------------------|-------------|-------|
------------------------------------------------------------------------
------------------------------------------------------------------------
Event Value Time
|------------------------------------------------|-------------|-------|
------------------------------------------------------------------------
------------------------------------------------------------------------
Event Value Time
|------------------------------------------------|-------------|-------|
------------------------------------------------------------------------
------------------------------------------------------------------------
Event Value Time
|------------------------------------------------|-------------|-------|
Fixed Peak Width 0.040 35.000

Order #: RMA20951 P368367.3
8 Qualification results
8.1 Cutting Time Determination C16-C18
Running sample 00.02.615, using the method C16-C18 Cutting Times, will yield a
chromatogram from which the correct cutting times to be used for C16 and C18
measurement can be determined. These cutting times need to be entered into method
C16-C18 Full Method.
FID1 A, Front Signal (D191209\S191209A 2019-12-09 16-28-48\B-001-201-00.02.615 C14 cutting time.D)
pA
70
60
50
40
30
20
10
-10
0 2.5 5 7.5 10 12.5 15 17.5 min
FID2 B, Back Signal (D191209\S191209A 2019-12-09 16-28-48\B-001-201-00.02.615 C14 cutting time.D)
pA
/3
70 1/2
18:
C
60 0
16:
C
50 0
17:
C
40 0
18:
C
30
20
10
-10
0 2.5 5 7.5 10 12.5 15 17.5 min
NOTE: When the cutting times are entered into the method, make sure that the refocus
cooling trap is activated 2 minutes before starting the 1st heart-cut (C16:0). This way the
refocus trap is cold enough (below 25 Celsius) at the moment that the 1st FAME component
C16:0 reaches it.

Order #: RMA20951 P368367.3
8.2 RF check 1st dimension column

pA
70
60
50
40
30
20
10
-10
0 2.5 5 7.5 10 12.5 15 17.5 min
pA
/3
70 1/2
18:
C
60 0
16:
C
50 0
17:
C
40 0
18:
C
30
20
10
-10
0 2.5 5 7.5 10 12.5 15 17.5 min
If the found response factors for the different peaks are running too far apart (by roughly
more than 25%), this can be an indication that the glass liner in the inlet is getting too
active and needs replacement.

Order #: RMA20951 P368367.3
Data File C:\Chem32\2\Data\D191209\S191209A 2019-12-09 16-28-48\B-001-201-00.02.615 C14

cutting time.D
Sample Name: 00.02.615 C14 cutting time
Trace Fame Avtur 12/10/2019 3:28:21 PM SYSTEM
=====================================================================
Acq. Operator : SYSTEM Seq. Line : 1
Sample Operator : SYSTEM
Acq. Instrument : Trace Fame Avtur Location : 201 (B)
Injection Date : 12/9/2019 4:30:20 PM Inj : 1
Inj Volume : 2.5 µl
Acq. Method : C:\Chem32\2\Data\D191209\S191209A 2019-12-09 16-28-48\IP599 C16 C18
Cutting
Time.M
Last changed : 11/1/2019 9:00:06 AM by SYSTEM
Analysis Method : C:\Chem32\2\Data\D191209\S191209A 2019-12-09 16-28-48\IP599 C16 C18
Cutting
Time.M (Sequence Method)
Last changed : 12/10/2019 3:28:11 PM by SYSTEM
(modified after loading)
=====================================================================
External Standard Report
=====================================================================
Sorted By : Signal
Calib. Data Modified : 12/10/2019 3:28:11 PM
Multiplier : 1.0000
Dilution : 1.0000
Use Multiplier & Dilution Factor with ISTDs
Signal 2: FID2 B, Back Signal
RetTime Type Area Amt/Area Amount Grp Name

[min] [pA*s] [mg/kg]
-------|------|----------|----------|----------|--|------------------
8.557 BB 277.30508 3.84181e-1 106.53526 C16:0
11.006 BB 280.40475 3.88162e-1 108.84239 C17:0
13.402 VB 574.03052 3.80255e-1 218.27801 C18:1/2/3
14.311 BB 281.93829 3.80161e-1 107.18201 C18:0
Totals : 540.83768
=====================================================================
*** End of Report ***

Order #: RMA20951 P368367.3
8.3 Cutting Time Determination C14

Running sample 00.02.615, using the method C14 Cutting Times, will yield a
chromatogram from which the correct cutting times to be used for C14 measurement can
be determined. These cutting times need to be entered into the C14 Full Method.
pA
90
80
70
60
50
40
30
20
10
0
0 5 10 15 20 25 30 35 min
pA
90
80
70
60
50
0
4:
40
C1
30
20
10
0
0 5 10 15 20 25 30 35 min
NOTE: When the cutting times are entered into the method, make sure that the refocus
cooling trap is activated 2 minutes before starting the 1st heart-cut (C14:0). This way the
refocus trap is cold enough (below 25 Celsius) at the moment that the 1st FAME component
C14:0 reaches it.

Order #: RMA20951 P368367.3
8.4 Calibration
8.4.1 C16-C18 (00.02.614)
Chromatogram
FID1 A, Front Signal (D191029D\S191206A 2019-12-06 17-04-20\B-021-201-00.02.614.D)
pA
12
11
10
9
C16:0
8
C18:0
7 C18:1
C18:2
6
C18:3
5
C18:1
26 28 30 32 34 36 38 min
FID2 B, Back Signal (D191029D\S191206A 2019-12-06 17-04-20\B-021-201-00.02.614.D)
pA
12
11
10
26 28 30 32 34 36 38 min

Order #: RMA20951 P368367.3
ChemStation report
Data File C:\Chem32\2\Data\D191029D\S191206A 2019-12-06 17-04-20\B-021-201-00.02.614.D
Sample Name: 00.02.614
=====================================================================
Injection Date : 12/7/2019 11:10:21 AM Inj : 1
Acq. Method : C:\Chem32\2\Data\D191029D\S191206A 2019-12-06 17-04-20\IP599 C16 C18.M
Analysis Method : C:\Chem32\2\Data\D191029D\S191206A 2019-12-06 17-04-20\IP599 C16 C18.M (
Sequence Method)
=====================================================================
=====================================================================
Sorted By : Signal
Multiplier : 1.0000
Dilution : 1.0000
Signal 1: FID1 A, Front Signal

Uncalibrated Peaks : using compound C18:1

[min] [pA*s] [ppm Wt]
-------|------|----------|----------|----------|--|------------------
26.849 BB 12.73466 3.78289e-1 4.81738 C16:0
31.146 BB 15.51415 3.31289e-1 5.13967 C18:0
32.077 BV 2.59355 3.84691e-1 9.97713e-1 C18:1
32.439 VB 12.29824 3.85273e-1 4.73818 C18:1
32.709 - - - C18:1
34.919 BB 9.09969 4.77725e-1 4.34715 C18:2
38.240 BB 8.52310 5.03581e-1 4.29207 C18:3
Totals : 24.33217
1 Warnings or Errors :
Warning : Calibrated compound(s) not found
=====================================================================

Order #: RMA20951 P368367.3
ChemStation Noise report

=====================================================================
Sequence File : C:\Chem32\2\Data\D191029D\S191206A 2019-12-06 17-04-20\S191206A.S
Sequence Method)
=====================================================================
Column(s)
=====================================================================
Column Description : 2nd Dimension Column FAME in A

Inventory# : autoID-3
Model# : 10.73.138 Manufacturer: PAC
Diameter : 250.00 µm Length : 60.0 m
Film thickness : 0.20 µm Void time : 1.227 min
# Injections : 0
Maximum Temperature: 275.0 °C
Comment :
Column Description : 1st Dimension Column FAME in A

# Injections : 0
Comment :
Column Description : Monitor Column FAME in AVTUR

# Injections : 0
Comment :
=====================================================================
External Standard Report with Performance and Noise
=====================================================================
Calib. Data Modified : 12/9/2019 11:14:12 AM

Multiplier : 1.0000
Dilution : 1.0000
Noise determination:
Time range Noise Noise Noise

from | to | (6*SD) | (PtoP) | (ASTM) | Wander | Drift
[min] | [min] | [pA] | [pA] | [pA] | [pA] | [pA/h]
-------|-------|---------|---------|---------|---------|---------
26.250 26.340 5.586e-2 6.390e-2 - - 6.841
30.800 30.900 2.963e-2 2.223e-2 - - 1.320
31.700 31.800 2.903e-2 2.120e-2 - - -4.950
34.500 34.600 2.880e-2 2.785e-2 - - -4.500
37.800 37.900 3.882e-2 3.191e-2 - - 8.347
10.750 10.850 3.256e-2 2.818e-2 - - 2.701e-1

Order #: RMA20951 P368367.3

-------|-------|---------|---------|---------|---------|---------
26.250 26.340 3.431e-2 2.866e-2 - - -2.138
30.800 30.900 3.532e-2 2.872e-2 - - -1.689e-1
31.700 31.800 3.049e-2 2.404e-2 - - -9.807e-1
34.500 34.600 3.985e-2 3.161e-2 - - -1.803
37.800 37.900 3.029e-2 2.039e-2 - - -3.392e-1
10.750 10.850 7.326e-2 5.201e-2 - - -28.026
RetTime k' Sig Amount Symm. Width Plates Signal Name

[min] [ppm Wt] [min] /Noise
-------|------|---|----------||-----|-------|-------|------|---------
26.849 20.88 1 5.06000 0.99 0.0508 1545614 70.7 C16:0
31.146 24.38 1 5.01000 0.99 0.0767 914316 108.2 C18:0
32.077 25.14 1 1.07132 0.55 0.2025 139007 7.0 C18:1
32.439 25.43 1 5.08000 0.94 0.0842 822899 75.3 C18:1
32.700 - 1 not f 0.00 - - - C18:1
34.919 27.45 1 4.99000 1.06 0.0933 775505 52.2 C18:2
38.240 30.16 1 5.07000 1.02 0.1042 746632 33.3 C18:3
=====================================================================

Order #: RMA20951 P368367.3
8.4.2 C14 (00.02.614)

Chromatogram
FID1 A, Front Signal (D191029D\S191206A 2019-12-06 17-04-20\B-033-201-00.02.614 C14.D)
pA
25
20
0
15
14:
C
-
54
.2
10
41
8
.54
5
39
0
39 39.5 40 40.5 41 41.5 42 42.5 min
FID2 B, Back Signal (D191029D\S191206A 2019-12-06 17-04-20\B-033-201-00.02.614 C14.D)
pA
25
20
15
10
0
39 39.5 40 40.5 41 41.5 42 42.5 min

Order #: RMA20951 P368367.3
ChemStation report
Data File C:\Chem32\2\Data\D191029D\S191206A 2019-12-06 17-04-20\B-033-201-00.02.614 C14.D
Sample Name: 00.02.614 C14
=====================================================================
Acq. Method : C:\Chem32\2\Data\D191029D\S191206A 2019-12-06 17-04-20\IP599 C14.M
Analysis Method : C:\Chem32\2\Data\D191029D\S191206A 2019-12-06 17-04-20\IP599 C14.M
(Sequence
Method)
=====================================================================
=====================================================================
Sorted By : Signal
Calib. Data Modified : Monday, December 9, 2019 11:13:12 AM
Multiplier : 1.0000
Dilution : 1.0000

-------|------|----------|----------|----------|--|------------------
41.254 BB 26.47661 1.93378e-1 5.12000 C14:0
Totals : 5.12000
=====================================================================

Order #: RMA20951 P368367.3
ChemStation Noise report

Data File C:\Chem32\2\Data\D191029D\S191206A 2019-12-06 17-04-20\B-033-201-00.02.614 C14.D
Sample Name: 00.02.614 C14
=====================================================================
Acq. Method : C:\Chem32\2\Data\D191029D\S191206A 2019-12-06 17-04-20\IP599 C14.M
Analysis Method : C:\Chem32\2\Data\D191029D\S191206A 2019-12-06 17-04-20\IP599 C14.M
(Sequence
Method)
=====================================================================
Column(s)
=====================================================================
Column Description : 2nd Dimension Column FAME in A

# Injections : 0
Comment :
Column Description : 1st Dimension Column FAME in A

# Injections : 0
Comment :
Column Description : Monitor Column FAME in AVTUR

# Injections : 0
Comment :
=====================================================================
External Standard Report with Performance and Noise
=====================================================================
Calib. Data Modified : Monday, December 9, 2019 11:13:12 AM

Multiplier : 1.0000
Dilution : 1.0000

-------|-------|---------|---------|---------|---------|---------
41.000 41.100 3.451e-2 2.813e-2 - - 11.585

Order #: RMA20951 P368367.3

-------|-------|---------|---------|---------|---------|---------
41.000 41.100 3.228e-2 2.400e-2 - - -1.034
RetTime k' Sig Amount Symm. Width Plates Signal Name

[min] [ppm Wt] [min] /Noise
-------|------|---|----------||-----|-------|-------|------|---------
41.254 35.94 1 5.12000 0.99 0.0842 1331014 143.6 C14:0
=====================================================================

Order #: RMA20951 P368367.3
8.5 Validation
8.5.1 C16-C18 (00.02.616)
Chromatogram
pA
12
11
10
6
C18:1
C18:2
C16:0
C18:0
C18:3
4
26 28 30 32 34 36 38 min
pA
12
11
10
26 28 30 32 34 36 38 min

Order #: RMA20951 P368367.3
ChemStation report
=====================================================================
Sequence Method)
=====================================================================
=====================================================================
Sorted By : Signal
Multiplier : 1.0000
Dilution : 1.0000


-------|------|----------|----------|----------|--|------------------
26.844 BB 6.91094e-1 6.93682e-1 4.79400e-1 C16:0
31.143 BB 7.89465e-1 1.83194e-1 1.44625e-1 C18:0
32.095 - - - C18:1
32.432 BV 7.25465 4.03631e-1 2.92820 C18:1
32.709 - - - C18:1
34.910 BB 1.91191 7.30033e-1 1.39576 C18:2
38.235 BB 7.10118e-1 1.45757 1.03505 C18:3
Totals : 5.98303
1 Warnings or Errors :
Warning : Calibrated compound(s) not found
=====================================================================

Order #: RMA20951 P368367.3
8.5.2 C16-C18 (00.02.617)

Chromatogram
pA
C18:1
35
30
25
20
15
C18:2
C16:0
10
C18:3
C18:0
C18:1
C18:1
0
26 28 30 32 34 36 38 min
pA
35
30
25
20
15
10
0
26 28 30 32 34 36 38 min

Order #: RMA20951 P368367.3
ChemStation report
=====================================================================
Sequence Method)
=====================================================================
=====================================================================
Sorted By : Signal
Multiplier : 1.0000
Dilution : 1.0000


-------|------|----------|----------|----------|--|------------------
26.844 BB 12.88238 3.78082e-1 4.87059 C16:0
31.136 BB 9.45322 3.26199e-1 3.08363 C18:0
32.070 BV 11.41631 3.84691e-1 4.39175 C18:1
32.442 VV 156.82448 3.60938e-1 56.60388 C18:1
32.685 VV 8.30503 3.84691e-1 3.19487 C18:1
33.999 BV 6.92318e-1 8.27934e-1 5.73194e-1 ?
34.442 BB 7.25515e-1 8.06472e-1 5.85107e-1 ?
34.723 BV 5.87985e-1 9.11167e-1 5.35752e-1 ?
34.902 VB 40.66716 4.25629e-1 17.30914 C18:2
38.215 BB 15.02760 4.66050e-1 7.00362 C18:3
Totals : 98.15153
=====================================================================

Order #: RMA20951 P368367.3
9 Spares and Consumables

9.1 CCG4800.100 Spare kit
Component part no Part Description Quantity
10.11.124 VALVE, 2PRT, 3MM ID. 1
10.52.029 DEACTIVATED FUSED SILICA, 5M X 0.1MM ID 1
20.44.001 T-PIECE, 1/4", REFOCUS TRAP 1
80102.300 VALVE, 24V 3-PORT, 1/16" ASSY 1
9.2 CCG4800.200 Consumable kit

Component part no Part Description Quantity
10.30.210 GC,FILTER,TRIPLE FOR BASE 1
COLUMN, CAPILLARY: SP, 60M X 250UM X 0.2UM, POLY
10.73.138 POLAR 1
COLUMN, CAPILLARY: PDS, 30M X 250UM X 1UM, METAL,
10.73.154 NONPOLAR 1
70.40.500 AC MICRO FLUIDIC NUT 3
20001.566 Sample Box Cal Mix 5ppm FAME in AVTUR (5pcs) 1
20001.567 Sample Box Cal Mix 100ppm FAME in AVTUR (5pcs) 1
20001.568 Sample Box Reference 5ppm FAME in AVTUR (5pcs) 1
20001.568 Sample Box Reference 100ppm FAME in AVTUR (5pcs) 1
21040.008 FERRULE, GRAPHITE FOR 0.25/0.32MM CAP.COL. (10 PCS) 1
21040.023 SILTITE FERRULES, 0.32MM COLUMN, 10PCS 1
ALS SYRINGE, 5UL TAPERED, FIXED NEEDLE 23-26S/42/HP
23020.075 (2 PCS) 1
21040.005 SEPTA, 11 MM (50 PCS) 1
5183-4647 LINER,SPLIT,LOW PRS DROP TPR D 10
Information
The information contained within these materials is subject to change without notice. PAC makes no warranties or
guarantees, either expressed or implied in these materials, including the warranty of merchantability or fitness for a
particular purpose. At any time, PAC may modify these materials, its instruments or its programs without notice and
subsequent versions of these materials may contain different information. These materials could contain technical
inaccuracies and/or typographical errors. PAC does not assume responsibility for the accuracy of any translation of these
materials.

AC FAME in AVTUR on Agilent 7890

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AC FAME in AVTUR on Agilent 7890

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AC FAME

Office: +31 (0) 10 – 4624 811

Rotterdam, December 10, 2019

Congratulations on the purchase of your AC Analytical Controls FAME in AVTUR Gas

With kind regards,

PAC L.P. (Corporate Headquarters)

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1.2 Analysis description

AC FAME in AVTUR Rev. 1.0

Column 1 = 10.73.154 1st Dimension Column FAME in AVTUR

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2.1 Leak testing

2.3.2 Retention Time Repeatability

2.3.3 Area Repeatability

LOQ = (10 * c * N * W * 60) / A

LOQ = Limit of Quantification (mg/kg)

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2.3.5 Peak Symmetry

1st Dimension Peak Symmetry

2nd Dimension Peak Symmetry

2.3.6 Validation results

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4 Actual Settings of the Analyzer

4.1 Pressures and Flows

Pressures [kPa] Back Inlet 410

Flows Back Inlet Split Flow 39.3

Analysis Column (Front FID) 4.1

Front FID Fuel Gas 29.6

Back FID Fuel Gas 29

Temperature setpoints Oven 230

Signal pA (20Hz) Front Detector 4.9

4.2 PID Settings GC

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Component AC Part No. Description

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Column positions inside GC oven

Component AC Part No. Description Max. Temp. (°C)

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6 Sample preparation and sample handling

6.1 Validation check

Method Cutting Time Sample Calibration Sample Validation Sample

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7 ChemStation method description

Method: C:\Chem32\2\Methods\IP599 C16 C18 Cutting Time.M

Run Time Checklist

Pre-Run Cmd/Macro: off

Standard Data Analysis: on

Modify Custom Fields: off

Customized Data Analysis: off

Save GLP Data: off

Post-Run Cmd/Macro: off

Save Method with Data: off

Injection Source and Location

Injection Source: GC Injector

Injection Location: Back

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Front Detector FID

Back Detector FID

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