LAPORAN PRAKTIKUM MIKROBIOLOGI INDUSTRI

MODUL : Sterilization Technique and Aseptic

KELOMPOK : (4 / hari praktikum)

Nama Praktikan : Adlina Firza
NRP : 5008211142
Nama Praktikan : Rayhan Shahreza Ferdian Ferryanto
NRP : 5008211143
Nama Praktikan : Kresna Ramadhani Fauzan
NRP : 5008201072

ASISTEN
Nama Asisten : Adidoyo Prakoso
NRP : 02211940000105
Nama Asisten :
NRP :

DOSEN PENGAMPU
Nama Dosen Pengampu :
NIP :

Tanggal Praktikum :
Tanggal Pengumpulan Laporan :

LABORATORIUM KIMIA FISIKA DAN MIKROBIOLOGI INDUSTRI

DEPARTEMEN TEKNIK KIMIA - FAKULTAS TEKNOLOGI INDUSTRI DAN
REKAYASA SISTEM INSTITUT TEKNOLOGI SEPULUH NOPEMBER
1. Objective

1. Recognize several ways of sterilization.

2. Understand the purpose of sterilization and disinfection.
3. Perform physical and chemical sterilization, and can perform aseptic work.
4. Obtain sterile tools and media.
5. Knowing the types of media and how to make them.
6. Recognize and know the function of microbial growth media.

2. Basic Theory

Disinfectants are chemicals used to inhibit or kill microorganisms on inanimate

surfaces. Disinfectants cannot be used on the skin or mucous membranes, because their
properties that damage tissues have the risk of irritating the skin and potentially triggering
cancer (Rutala, 2019).
Antiseptics are non-toxic disinfectants because they are applied to the skin, mucosa or
other living tissues. Antiseptics have several requirements, among others, have a broad
spectrum, do not stimulate the skin or mucosa, toxicity or absorption through the skin and
mucosa is low, the effect is fast and lasts a long time (Septiari, 2012).
The effectiveness or power of a disinfectant is influenced by concentration, pH,
contact time, age of bacteria, temperature, media, and solvent used. For example, at slightly
higher concentrations the fungicidal effect is stronger than the bactericidal effect. At pH 6 the
effect of chlorhexidine is 10 times stronger than at pH 9, also benzoic acid and its esters are
more active at acidic pH. Another factor that affects the effectiveness of antiseptics is the
number of microbes, where the more the more time it takes to kill them. The culture of
microorganisms used in the antiseptic-disinfectant test requires nutrients as a growth medium
that contains nutrients and a suitable growth environment for microorganisms (W. Lay,
1994).
Growth media is a nutrient medium that is prepared to grow bacteria. Some bacteria
can grow well on any medium and some bacteria require special media. The media must be
able to provide the energy needed for bacterial growth (Radji, 2010).

6. References

Donnenberg. 2013. Escherichia Coli Pathotypes and Principles of Pathogenesis. Baltimore:

University of Maryland School of Medicine Academic Press
Nasution,M. 2012. Pengantar Mikrobiologi. Medan : USU. 76-77

Radji, Maksum. 2010. Buku Ajar Mikrobiologi Panduan Mahasiswa Farmasi dan
Kedokteran. Jakarta: EGC.