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HPLC Analysis of Spermine and Related Compounds on Amaze TR HILIC and Ion-
Exchange Modes

HPLC Analysis of Spermine and Related Compounds on Amaze TR Mixed-Mode


Column
Column

HPLC Analysis of Spermine and Related Compounds on Amaze TR


HILIC and Ion-Exchange Modes

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Conditions of Experiment
C l A TR
Column: Amaze TR

Separation Modes: HILIC, cation-exchange

Column Dimenstions: 3.0x50 mm, 3 um, 100A

Mobile Phase: A - 75% ACN with 5 mM AmFm pf 3

B - 20% ACN with 20 mM AmFm pH 2.1

from 100% A to 100% B in 4 min, 2 min hold

Detection: ELSD, MS

Sample: 0.5-0.8 mg/ml each

Injection: 3 uL

Flow rate: 0.7 ml/min

Analytes
Class of compounds: Amines, Polyamine, Polymer

Nature of compounds: Basic, Hydrophilic

Compounds: Putrescene, Spermidine, Spermine

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