913122, 1752 Transgenic Fly Virual Lab Lab Notebook Part 1: Quiz answers & Print 4. In this experiment, the reporter gene ‘A. tells the experimenter exactly how much of the native protein was produced B. changes the color of the flies’ eyes C. must be from the same type of organism as the organism into which it is being transferred D. is designed to be easily detected and reflect the activity of the native gene Correct! Your answer: D. ‘A. INCORRECT. Levels of native protein (in this case, endogenous period protein) are not reported by the reporter gene. B. INCORRECT, The reporter gene in this experiment is luciferase, which produces light in amounts proportional to the transcriptional activity of the per promoter. The marker gene mini-white is responsible for the red eye color in those flies that have incorporated the DNA construct ©. INCORRECT. Reporter genes can originate from many sources, For example, the firefly luciferase gene has been inserted into plants, and the gene for the jellyfish green fluorescent protein has been inserted into mice. D. CORRECT. Reporter genes are designed to be easily measured and quantified, either by eye or by machines. 2. After successfully incorporating into the genome of a fly, the construct DNA will ‘A. change the functions of the native per gene B. cause the eyes to be white C. reflect transcriptional activity without interfering with functions D. alter the biological clock Correct! Your answer: C. C. CORRECT. The transgenic fly is expected to have normal per gene activity and a normal biological clock. Two differences occur as a result of incorporation: the transgenic fly has red eyes and per transcriptional activity is reported as light ‘emissions. A,B, and D. INCORRECT. The transgenic fly is expected to have normal per gene activity and a normal biological clock. Two differences occur as a result of incorporation: the transgenic fly has red eyes and per transcriptional activity is reported as light emissions. 3. Both fireflies and per-luc transgenic Drosophila ‘A. can be seen glowing outdoors at dusk B, produce the enzyme luciferase C. use their glow to attract mates D. are experimentally produced hips simedia.nhmiorgicileractivehlabsiransgenic_fyindex himl 42sa/sza, 1752 “Transgenic Fly Viual Lab Lab Notebook Correct! Your answer: B. ‘A. INCORRECT. Even an escaped transgenic Drosophila would not glow enough to be visible with the naked eye. B, CORRECT. Both produce the enzyme luciferase. The natural glow of fireflies is visible to humans. However, the transgenic Drosophila glow only when the chemical luciferin, the substrate for luciferase, is provided in their diet. C. INCORRECT. Fireflies use their glow to attract mates, but Drosophila do not naturally glow. D. INCORRECT. Drosophila can be designed in the lab to glow. Fireflies have the natural ability to glow. Some research labs do study fireflies to understand the chemical processes responsible for bioluminescence. 4, Which of the following DNA sequences is not found in the “construct DNA?" A. transposase B. luciferase C. period gene promoter D. P element Correct! Your answer: A. ‘A. CORRECT. Transposase is found in the transposase DNA vector, not in the construct DNA, B, C, and D. INCORRECT. All are found in the construct DNA vector. View lab status and quiz summaries hitpssimedia.nhmi orgiicinleractivehlabsiransgenic_fyindex nimi 22.