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    8 views9 pages

    Interim - Biosafety Guidelines - Monkeypox - BRMO - Ver2 PDF

    Uploaded by

    Alexandra Regilyne Romero

    Copyright:

    © All Rights Reserved
    Download as PDF or read online from Scribd
    Flag for inappropriate content
    of 9
    Research Institute for Tropical Medicine - Department of Health '9002 Research Drive, Filinvest Corporate City, Alabang, Muntinlupa City, 1781 Philippines Tet Non; (632) 8609-7599 /8807-2631/32/37 * Website: wwaitim.gov-ph Biorisk Management Office Interim Laboratory Biosafety Guidelines for Handling and Processing ‘Suspected Monkeypox Specimens Ver 2-220801 Introduction Cases of Monkeypox have been recently detected in countries that are not endemic forits actiologic agent - the Monkeypox virus. Monkeypox virus is an enveloped double-stranded ONA virus (Orthopoxvirus genus of the Poxviridae family). Monkeypox virus belongs to the genus that includes variola virus/smallpox vaccinia virus, and cowpox virus. Monkeypox virus is a Risk Group 3 pathogen and listed as an Infectious Substance Category A (specimen and culture) , a sub-classification under IATA Dangerous Good Class 6.2 with UN Number 2814 and proper shipping name Infectious substance, affecting human Monkeypox is a rare viral zoonotic disease whose first human case was initially reported in Democratic Republic of the Congo (ORC) and in several other central and western African countries. The natural reservoir of monkeypox remains unknown. African rodents and non-human primates (ike monkeys) may harbor the virus and infect people. Monkeypox typically presents clinically with fever, rash and swollen lymph nodes and may lead to a range ‘of medical complications. The clinical presentation of monkeypox resembles that of smallpox, however less contagious and commonly presents a less severe illness. Monkeypox isa self-limited disease with the symptoms lasting from 2 to 4 weeks. In recent times, the case fatality ratio has been around 3-6%. Monkeypox is transmitted to humans through close contact with an infected person or animal, or with material contaminated with the virus (e.g., lesions, body fiuids, respiratory droplets and other ‘contaminated materials. Outside Africa, transmission i inked to international travel or imported animals. ‘An antiviral agent developed has been licensed for the treatment of monkeypox. Vaccines have been developed and approved for prevention of monkeypox. ‘The Pathogen Safety Data Sheet listed ingestion, parenteral inoculation, droplet or aerosol exposure of mucous membranes or broken skin, or contact with infectious fluids oF tissues as the primary hazard in laboratory. Research Institute for Tropical Medicine - Department of Health '9002 Research Drive, Flinvest Corporate City, Alabang, Muntinlupa City, 1781 Philippines Tel Nox: (632) 609-7599 /8807-2631/22/37 » Webnite: wwmalimgovoh General Biosafety Guidelines 1. Local biorisk assessment must be conducted to determine specific mitigation control measure towards an effective biorisk management. Laboratory practices, equipment, primary and secondary containment could be enhanced based on local risk assessment. 2. At minimum, laboratory must strictly enforce compliance with Biosafety Level 2 Standards as core biosafety requirement. BMBL 5® Edition hitps://www.cde nov/lnbs/ndi/CDC BiosafetyMicrobiologicalBiomedicallaboratories-2009-P.PDF ‘* Both routine and special laboratory diagnostic procedures using specimen taken from a confirmed or suspected Monkeypox patients must be handled in BSL 2 laboratory with enhancement in practices and PPE similar to those described in a BSL3 laboratory. ‘© Invitro and in vivo propagative activities (e.g, viral culture, animal inoculation and other Viral isolation techniques) and concentration of the agent being investigated (Monkeypox) is strictly prohibited and strongly discouraged. These procedures can only be conducted in a certified BSL 3 laboratory with highly trained laboratory personnel. 3. Use of Class Il Biosafety Cabinet is required for manipulation of monkeypox specimen inside laboratory + Procedures with high potential (likelihood) of generating fine-particulate aerosols must be performed in a functional, well-maintained certified Class I! Biological Safety Cabinet (BSC I). The procedures that may generate eerosol includes vortex mixing, pipetting, opening primary containers after vigorous mixing and other related procedure that applies pressure to specimen containing or potentially containing the viral agents being investigated. 4. Appropriate physical containment devices like safety centrifuge buckets or sealed rotors must be used for centrifugation. Safety centrifuge buckets must be sealed and must be loaded and 5. Laboratory workers must wear the prescribed Personal Protective Equipment (PPE) based on local risk assessment. © The prescribed minimum PPE: Gloves, scrub suits, gown (isolation / solid front or coverall), face protection (surgical mask, goggles / face shield), respiratory protection (e.g. N95 or its equivalent), dedicated laboratory shoes / shoe cover ‘© Snug fitting goggles are preferred, face shield should have crown and chin protection plus ‘wrap around the face to the point of the ear to provide a barrier to mucosal surface exposure. * Respiratory protection is essential since the virus is known to spread naturally via inhalation routes, It is recommended to use a properly fit-tested, NIOSH-approved filtering face piece respirator (N-95) or a powered air-purifying respirator (PAPR) equipped with high-efficiency particulate air (HEPA) filters in case of falled Respirator Fit Test. Personnel must be evaluated properly by a medical doctor for fitness to use of a respirator. | (A)= ge Research Institute for Tropical Medicine - Department of Health 9002 Research Drive, Filinvest Comporate City, Alabang, Muntinlupa City, 17B1 Philippines Tel Nos. (632) 8809-7599 / 8807-2631/32/37 © Website: www.itm.govph + No potentially contaminated PPE shall be taken out of laboratory for independent/personal washing/cleaning. PPE Matrix Gloves | Sub] talation | Covel’ | Foot cover | Gonale | Sreal | Respirator suits | gown | gown | /Ladshoes | /Tacesbield | Mask “Specimen * Collection

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