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    1. The order of UV/Vis absorption bands from shortest to longest wavelength for the π→π* transition is benzene, toluene, phenol, 3-nitrophenol, 4-nitrophenol. Substituents on the benzene ring cause shifts in wavelength due to their electron-withdrawing or -donating effects. 2. UV/Vis spectroscopy can be used for protein quantitative analysis because proteins contain aromatic amino acids that absorb UV/Vis light, allowing protein concentration to be determined from absorption measurements. 3. A chemiluminescence method could analyze protein content in milk powder by using a chemical reaction that produces light upon interaction with proteins, then measuring the

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    1. The order of UV/Vis absorption bands from shortest to longest wavelength for the π→π* transition is benzene, toluene, phenol, 3-nitrophenol, 4-nitrophenol. Substituents on the benzene ring cause shifts in wavelength due to their electron-withdrawing or -donating effects. 2. UV/Vis spectroscopy can be used for protein quantitative analysis because proteins contain aromatic amino acids that absorb UV/Vis light, allowing protein concentration to be determined from absorption measurements. 3. A chemiluminescence method could analyze protein content in milk powder by using a chemical reaction that produces light upon interaction with proteins, then measuring the

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    Uploaded by

    姜品瑜
    1. The order of UV/Vis absorption bands from shortest to longest wavelength for the π→π* transition is benzene, toluene, phenol, 3-nitrophenol, 4-nitrophenol. Substituents on the benzene ring cause shifts in wavelength due to their electron-withdrawing or -donating effects. 2. UV/Vis spectroscopy can be used for protein quantitative analysis because proteins contain aromatic amino acids that absorb UV/Vis light, allowing protein concentration to be determined from absorption measurements. 3. A chemiluminescence method could analyze protein content in milk powder by using a chemical reaction that produces light upon interaction with proteins, then measuring the

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    1.

    Show the order (from short to long wavelength) for the UV/Vis absorption band (π
    →π*) of benzene, toluene, phenol, 3-nitrophenol, 4-nitrophenol. Explain your
    answer.
    2. UV/Vis can be used for protein quantitative analysis. Explain why?
    3. Please propose a chemiluminescence method for analyzing protein content in a
    sample of milk powder.
    4. Explain why 8-hydroxyquinoline can be used to detect aluminum ion by
    fluorescence spectroscopy.
    5. The C=O stretching frequency of vaporized acetic acid varied with its
    concentration. Explain why.
    6. Draw all the vibrational modes for SO2.
    7. A He/Ne laser with a wavelength of 632.8 nm is usually equipped in an FT-IR
    spectrometry. Can we replace the He/Ne laser by a laser emitted 1000 nm
    radiation? Explain.
    8. Photoacoustic IR spectroscopy is rarely used. But some samples have to use
    photoacoustic IR spectroscopy to obtain a good quality IR spectrum. Describe
    properties of such samples.
    9. Attenuated total reflectance – Infrared spectroscopy (ATR-IR) has been used
    frequently. Ge, Si, and ZnSe are commonly used as ATR crystal for detection.
    Please compare the performances between these crystals in ATR-IR detection.
    10. What are the advantages of FT-Raman compared to dispersive Raman
    spectrometry?

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