Abstract

Proteolytic enzymes, also known as "proteases," cleave the peptide bonds that connect two
amino acids. They follow a hydrolytic reaction mechanism. Proteolytic enzymes are produced by both
prokaryotic and eukaryotic organisms in which they perform key biological functions. Proteolytic
enzymes (proteases) are enzymes that break down protein. These enzymes are made by animals, plants,
fungi, and bacteria. Proteolytic enzymes break down proteins in the body or on the skin. This might help
with digestion or with the breakdown of proteins involved in swelling and pain. The three main
proteolytic enzymes produced naturally in your digestive system are pepsin, trypsin and chymotrypsin. 

Results

This are the results in the three data table laboratory experiments. In Data Table A. Action of
Pepsin Milk the results of the experiment in conducted it was Hydrochloric acid are present in test tubes
A, B and C because in order to convert the pepsinogen into pepsin. Pepsin is an endopeptidase that
initiates the process of protein digestion. Pepsin action results in the release of small peptides and
amino acids. To the Data Table B. Action of Pepsin on Milk the experiment of the results in different
solutions are test tubes, such as A, pepsin solution with dilute hydrochloric acid was successful and able
to dissolve the egg white. In test tube B, the egg white was almost dissolved in the solution however
there are still residues left. The pH of distilled water affected the pepsin solution. While in Test tubes C
and D, the results are similar. Lastly, In Data Table C. Action of Trypsin on Gelatin the digestion of the
gelatin observed after 16 minutes the pH 7 strip is clearing and releasing the buffer solution. After 22
minutes pH1 is still strip is blank, pH4 still strip is black, pH7 is not clear, the pH10 strip still has gelatin
(proteins) on the ends and pH14 is still strip is black. The gelatin (protein) on the pH 1, 4 and 14 strips
has been digested.