You are on page 1of 1

Proyecto

Balance
Tuesday, 31 January 2023 2:10 PM

A method for lactic acid production and lipid extraction from microalgae
(Nannochloropsis salina) biomass was investigated. Microalgae biomass was acid (5%
H2SO4) hydrolyzed at 120 °C for 1 h, and subsequently treated with hexane at 40 °C
and 200 rpm to separate lipid from the hydrolysate. The sugar (glucose plus xylose)
yield reached 64.3% and remained the same until the biomass loading of 15% (w/v)
after which, it dropped. Lipid free hydrolysate was neutralized and used as
fermentation medium for Lactobacillus pentosus for lactic acid production. Lactic
acid yield reached 92.8% at sugar 3–25 g/l, 30 °C and shaking speed 150 rpm under
anaerobic condition. The acid hydrolysis facilitated lipid extraction from microalgae
biomass, and lipid yields with and without acid hydrolysis were 85.6% and 48.7%,
respectively. Results suggest that the developed method can be successfully applied
in lipid extraction and lactic acid production from microalgae biomass.

You might also like