NUCLEODUR? columns NUCLEODUR® Phenyl-Hexyl productive for polar/ aromatic compunds » USP L11 E3 key feature Technical data Hydrophobic phase with alternative selectivity compared to classical Cy» modifications + Separation principle based on 2 reten- tion mechanisms: n-r interactions and hydrophobic interactions + Suitable for LC/MS due to low blee- cing characteristics stabity 1-10 Phenyihexy! madied phases are an interesting alternative to classical Cx phases due to an excollant separation of aromatic and unsaturated compounds especially with electron wthdra- ‘wing groups. ‘The combination of hydrophobic and polar T-1 interactions re- sult in an interesting and ateriate selectivity in comparison to yg and Ce modtied phases, Through short ohenythexy! chains the NUCLEODUR® Phe- nybHexy Is more polar than the bifunctional modified NUGLEODUR® Sphinx RP. Therefore shorter analyss times can be achieved with mixtures of structural sinlar aromatic and alphatic unsaturated compounds. \With NUCLEODUR® Phenyi-Hexy! e.g, teyeie antidepressants lr water soluble vitarnins can be separated in good resolution, ‘Separation of watersol.ble itn on NUCLEODUR® Phenyl Hex MN Appl No. 125020 coker: 100.3 mm NUCLEODURY Phantom, 3m Ben: 290% phosphor ald n water 310.1% shospnore acidinaostonirle (0% Bfor2 mi, then ta 609% Bin 7 mh Flowrate: 0.88 muirin Tomporatre: 35°C Detection: Uv, 25 am econ 0.8 UL, 1.0 mgiml each compound 1 mgmt in eon Paks: a8 1 Thame 2. Pyrat 8. pramncoerane asd 4 Pantothenic aoc SiFoicacd ‘ 680i + Phase with phanyt-hexy! moaticat- ‘on and mut-endeapping; pore size 110 A; particle 820s 1.8 um, 3 um ‘and 5 um; carbon content 10%; pH Recommended application + Aromatic and unsaturated com- pounds, polar compounds like phar- ‘macouticals, antibiotics ‘cou: ont ow rate Toreratre: Detector ction: Pooks: 1. Potiptyine 2, Nori 8. migraine 4. Arteotyine 5. Tinpramioe 1 “Toyct amtepressant (TCA) [MN Appl No. 126020 1603.0 [NUCLEODUR* PhenytHenl 8 um Aglont ZORBEX* Eclpse Phones 8.5 ym PPonxrenex Luna® Phony Hox 3 un 819.1% formic ei in ater B)0.1Sfomic acl in water 20-92.5% Ain 40min 0.56 rum sore Uy, 254 nm 0.2 uL each compound 1 mgmt in ees: e |} sucrsooure |_ eae 3 ym ll 170 | QD) wee tc