ILNAS-EN ISO 6888-1:2021 - Preview only Copy via ILNAS e-Shop

ILNAS-EN ISO 6888-1:2021

Microbiology of the food chain -

Horizontal method for the
enumeration of coagulase-positive
staphylococci (Staphylococcus aureus
Mikrobiologie der Lebensmittelkette -
Horizontales Verfahren für die Zählung
von koagulase-positiven Staphylokokken
(Staphylococcus aureus und andere
Microbiologie de la chaîne alimentaire -
Méthode horizontale pour le
dénombrement des staphylocoques à
coagulase positive (Staphylococcus

09/2021
 ILNAS-EN ISO 6888-1:2021

National Foreword

This European Standard EN ISO 6888-1:2021 was adopted as Luxembourgish Standard

ILNAS-EN ISO 6888-1:2021.

Every interested party, which is member of an organization based in Luxembourg, can

participate for FREE in the development of Luxembourgish (ILNAS), European (CEN,
CENELEC) and International (ISO, IEC) standards:

- Participate in the design of standards

- Foresee future developments
- Participate in technical committee meetings
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https://portail-qualite.public.lu/fr/normes-normalisation/participer-normalisation.html

THIS PUBLICATION IS COPYRIGHT PROTECTED

Nothing from this publication may be reproduced or utilized in
any form or by any mean - electronic, mechanical, photocopying
or any other data carries without prior permission!
 
ILNAS-EN ISO 6888-1:2021
 EN ISO 6888-1
 
2
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ͳǣʹͲʹͳȌ
‹…”‘„‹‘Ž‘‰‹‡†‡Žƒ…ŠƒÁ‡ƒŽ‹‡–ƒ‹”‡Ǧ±–Š‘†‡  ‹”‘„‹‘Ž‘‰‹‡†‡”‡„‡•‹––‡Ž‡––‡Ǧ‘”‹œ‘–ƒŽ‡•
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†‡ƒ‹”†Ǧƒ”‡”ȋ͸ͺͺͺǦͳǣʹͲʹͳȌ ͸͸ͺͺͺǦͳǣʹͲʹͳȌ

Š‹•—”‘’‡ƒ–ƒ†ƒ”†™ƒ•ƒ’’”‘˜‡†„›‘ʹ͹’”‹ŽʹͲʹͳǤ

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–”ƒ•Žƒ–‹‘—†‡”–Š‡”‡•’‘•‹„‹Ž‹–›‘ˆƒ‡„‡”‹–‘‹–•‘™Žƒ‰—ƒ‰‡ƒ†‘–‹ˆ‹‡†–‘–Š‡Ǧƒƒ‰‡‡–
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‹Žƒ†ǡ ”ƒ…‡ǡ
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‘Žƒ†ǡ‘”–—‰ƒŽǡ‡’—„Ž‹…‘ˆ‘”–Šƒ…‡†‘‹ƒǡ‘ƒ‹ƒǡ‡”„‹ƒǡŽ‘˜ƒ‹ƒǡŽ‘˜‡‹ƒǡ’ƒ‹ǡ™‡†‡ǡ™‹–œ‡”Žƒ†ǡ—”‡›ƒ†
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CEN-CENELECManagement Centre: Rue de la Science 23, B-1040 Brussels

̹ʹͲʹͳ ŽŽ”‹‰Š–•‘ˆ‡š’Ž‘‹–ƒ–‹‘‹ƒ›ˆ‘”ƒ†„›ƒ›‡ƒ•”‡•‡”˜‡† ‡ˆǤ‘Ǥ͸ͺͺͺǦͳǣʹͲʹͳ

™‘”Ž†™‹†‡ˆ‘”ƒ–‹‘ƒŽ‡„‡”•Ǥ
 EN ISO 6888-1:2021 (E) ILNAS-EN ISO 6888-1:2021

Contents Page

European foreword....................................................................................................................................................... 3
ILNAS-EN ISO 6888-1:2021 - Preview only Copy via ILNAS e-Shop

2
 ILNAS-EN ISO 6888-1:2021 EN ISO 6888-1:2021 (E)

European foreword

This document (EN ISO 6888-1:2021) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 463 “Microbiology of the food chain” the
secretariat of which is held by AFNOR.

This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by March 2022, and conflicting national standards shall
be withdrawn at the latest by March 2022.

Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.

This document supersedes EN ISO 6888-1:1999.

ILNAS-EN ISO 6888-1:2021 - Preview only Copy via ILNAS e-Shop

Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN websites.

According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.

Endorsement notice

The text of ISO 6888-1:2021 has been approved by CEN as EN ISO 6888-1:2021 without any
modification.

3
 INTERNATIONAL
ILNAS-EN ISO 6888-1:2021
ISO
STANDARD 6888-1

Second edition
2021-08

Microbiology of the food chain —

Horizontal method for the
ILNAS-EN ISO 6888-1:2021 - Preview only Copy via ILNAS e-Shop

enumeration of coagulase-positive
staphylococci (Staphylococcus aureus
and other species) —
Part 1:
Method using Baird-Parker agar
medium
Microbiologie de la chaîne alimentaire — Méthode horizontale
pour le dénombrement des staphylocoques à coagulase positive
(Staphylococcus aureus et autres espèces) —
Partie 1: Méthode utilisant le milieu gélosé de Baird-Parker

Reference number
ISO 6888-1:2021(E)

© ISO 2021
 ISO 6888-1:2021(E) ILNAS-EN ISO 6888-1:2021
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COPYRIGHT PROTECTED DOCUMENT

© ISO 2021
ŽŽ”‹‰Š–•”‡•‡”˜‡†ǤŽ‡••‘–Š‡”™‹•‡•’‡…‹ϐ‹‡†ǡ‘””‡“—‹”‡†‹–Š‡…‘–‡š–‘ˆ‹–•‹’Ž‡‡–ƒ–‹‘ǡ‘’ƒ”–‘ˆ–Š‹•’—„Ž‹…ƒ–‹‘ƒ›
„‡”‡’”‘†—…‡†‘”—–‹Ž‹œ‡†‘–Š‡”™‹•‡‹ƒ›ˆ‘”‘”„›ƒ›‡ƒ•ǡ‡Ž‡…–”‘‹…‘”‡…Šƒ‹…ƒŽǡ‹…Ž—†‹‰’Š‘–‘…‘’›‹‰ǡ‘”’‘•–‹‰
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„‡Ž‘™‘”ǯ•‡„‡”„‘†›‹–Š‡…‘—–”›‘ˆ–Š‡”‡“—‡•–‡”Ǥ
…‘’›”‹‰Š–‘ˆϐ‹…‡
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ii © ISO 2021 – All rights reserved

 ILNAS-EN ISO 6888-1:2021 ISO 6888-1:2021(E)

Contents Page

Foreword ........................................................................................................................................................................................................................................ iv
Introduction................................................................................................................................................................................................................................ vi
1 Scope ................................................................................................................................................................................................................................. 1
2 Normative references ...................................................................................................................................................................................... 1
͵ ‡”•ƒ††‡ϐ‹‹–‹‘• ..................................................................................................................................................................................... 2
4 Principle ........................................................................................................................................................................................................................ 2
ͶǤͳ
‡‡”ƒŽ ........................................................................................................................................................................................................... 2
4.2 Incubation ................................................................................................................................................................................................... 2
ͶǤ͵ —‡”ƒ–‹‘ƒ†…‘ϐ‹”ƒ–‹‘ ................................................................................................................................................ 2
5 Culture media and reagents...................................................................................................................................................................... 3
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6 Equipment and consumables .................................................................................................................................................................. 3

7 Sampling........................................................................................................................................................................................................................ 4
8 Preparation of the test sample............................................................................................................................................................... 4
9 Procedure..................................................................................................................................................................................................................... 4
ͻǤͳ ‡•–’‘”–‹‘ǡ‹‹–‹ƒŽ•—•’‡•‹‘ƒ††‹Ž—–‹‘•.............................................................................................................. 4
9.2 Inoculation and incubation .......................................................................................................................................................... 4
9.3 Counting of colonies ........................................................................................................................................................................... 5
ͻǤ͵Ǥͳ
‡‡”ƒŽ†‡•…”‹’–‹‘‘ˆ…‘Ž‘‹‡•‰”‘™‹‰‘‡†‹— .......................................................... 5
9.3.2 Colony counting procedure .................................................................................................................................... 5
ͻǤͶ ‘ϐ‹”ƒ–‹‘ ............................................................................................................................................................................................. 6
ͻǤͶǤͳ
‡‡”ƒŽ...................................................................................................................................................................................... 6
9.4.2 Tube test.................................................................................................................................................................................. 6
9.4.3 Plate test using RPFA medium ............................................................................................................................. 7
10 Expression of results ........................................................................................................................................................................................ 7
11 Performance characteristics of the method............................................................................................................................. 8
11.1 Interlaboratory study ........................................................................................................................................................................ 8
11.2 Repeatability limit................................................................................................................................................................................ 8
11.3 Reproducibility limit .......................................................................................................................................................................... 8
12 Test report ................................................................................................................................................................................................................... 9
13 Quality assurance ................................................................................................................................................................................................ 9
Annex A (normative) Flow diagram of the procedure ..................................................................................................................... 10
Annex B (normative) Culture media and reagents ............................................................................................................................. 11
Annex C (informative) Results of the interlaboratory study..................................................................................................... 18
Bibliography ............................................................................................................................................................................................................................. 20

© ISO 2021 – All rights reserved iii