Reference Standards

Organochlorine Pesticide Mix AB #3

Catalog # 32415
Lot # A0137734
110 Benner Circle Bellefonte, PA 16823-8812
1-814-353-1300 1-800-356-1688
www.restek.com

FOR LABORATORY USE ONLY. READ SDS PRIOR TO USE.

RAW MATERIAL TEST INFORMATION AVAILABLE UPON REQUEST

MANUFACTURED UNDER RESTEK'S ISO 9001 REGISTERED QUALITY SYSTEM

By dshaffer at 5:47 pm, Jun 26, 2018 Page 1 of 7

 CERTIFIED REFERENCE MATERIAL
110 Benner Circle
Bellefonte, PA 16823-8812
Tel: (800)356-1688 Certificate of Analysis
Fax: (814)353-1309

www.restek.com

FOR LABORATORY USE ONLY-READ SDS PRIOR TO USE.

This Reference Material is intended for Laboratory Use Only as a standard for
the qualitative and/or quantitative determination of the analyte(s) listed.

Catalog No. : 32415 Lot No.: A0137734

Description : Organochlorine Pesticide Mix AB #3

Organochlorine Pesticide Mix AB #3 2,000 µg/mL, Hexane/Toluene (

50:50), 1mL/ampul

Container Size : 2 mL Pkg Amt: > 1 mL

Expiration Date : September 30, 2022 Storage: 10°C or colder
Handling: Sonicate prior to use.

C E R T I F I E D V A L U E S
Elution Compound Grav. Conc. Expanded Uncertainty
Order (weight/volume) (95% C.L.; K=2)

1 alpha-BHC 2,016.5 µg/mL +/- 11.8340 µg/mL Gravimetric

CAS # 319-84-6 (Lot 0911942) +/- 91.8500 µg/mL Unstressed
Purity 99% +/- 132.6190 µg/mL Stressed

2 gamma-BHC (Lindane) 2,018.5 µg/mL +/- 11.8457 µg/mL Gravimetric

CAS # 58-89-9 (Lot 6815700) +/- 91.9411 µg/mL Unstressed
Purity 99% +/- 132.7505 µg/mL Stressed

3 beta-BHC 2,009.5 µg/mL +/- 11.7928 µg/mL Gravimetric

CAS # 319-85-7 (Lot BCBS8692V) +/- 91.5307 µg/mL Unstressed
Purity 98% +/- 132.1579 µg/mL Stressed

4 delta-BHC 2,018.5 µg/mL +/- 11.8457 µg/mL Gravimetric

CAS # 319-86-8 (Lot ER02101401) +/- 91.9411 µg/mL Unstressed
Purity 99% +/- 132.7505 µg/mL Stressed

5 Heptachlor 2,015.0 µg/mL +/- 11.8251 µg/mL Gravimetric

CAS # 76-44-8 (Lot NT060133) +/- 91.7817 µg/mL Unstressed
Purity 99% +/- 132.5203 µg/mL Stressed

6 Aldrin 2,014.5 µg/mL +/- 11.8222 µg/mL Gravimetric

CAS # 309-00-2 (Lot 7321900) +/- 91.7589 µg/mL Unstressed
Purity 99% +/- 132.4874 µg/mL Stressed

7 Heptachlor epoxide (isomer B) 2,007.5 µg/mL +/- 11.7811 µg/mL Gravimetric

CAS # 1024-57-3 (Lot ER121511-01) +/- 91.4401 µg/mL Unstressed
Purity 99% +/- 132.0271 µg/mL Stressed

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8 trans-Chlordane 2,009.0 µg/mL +/- 11.7899 µg/mL Gravimetric
CAS # 5103-74-2 (Lot ER061906-04) +/- 91.5084 µg/mL Unstressed
Purity 99% +/- 132.1257 µg/mL Stressed

9 cis-Chlordane 2,008.0 µg/mL +/- 11.7841 µg/mL Gravimetric

CAS # 5103-71-9 (Lot ER062410-01) +/- 91.4628 µg/mL Unstressed
Purity 99% +/- 132.0599 µg/mL Stressed

10 Endosulfan I 2,016.0 µg/mL +/- 11.8310 µg/mL Gravimetric

CAS # 959-98-8 (Lot ER012105-02) +/- 91.8272 µg/mL Unstressed
Purity 99% +/- 132.5861 µg/mL Stressed

11 4,4'-DDE 2,012.5 µg/mL +/- 11.8105 µg/mL Gravimetric

CAS # 72-55-9 (Lot GHYQG) +/- 91.6678 µg/mL Unstressed
Purity 99% +/- 132.3559 µg/mL Stressed

12 Dieldrin 1,999.2 µg/mL +/- 11.7324 µg/mL Gravimetric

CAS # 60-57-1 (Lot 5709400) +/- 91.0620 µg/mL Unstressed
Purity 98% +/- 131.4812 µg/mL Stressed

13 Endrin 2,012.4 µg/mL +/- 11.8101 µg/mL Gravimetric

CAS # 72-20-8 (Lot 6898800) +/- 91.6646 µg/mL Unstressed
Purity 98% +/- 132.3513 µg/mL Stressed

14 4,4'-DDD 2,005.5 µg/mL +/- 11.7694 µg/mL Gravimetric

CAS # 72-54-8 (Lot HAN02-KNCK) +/- 91.3490 µg/mL Unstressed
Purity 99% +/- 131.8955 µg/mL Stressed

15 Endosulfan II 2,018.0 µg/mL +/- 11.8428 µg/mL Gravimetric

CAS # 33213-65-9 (Lot 7276300) +/- 91.9183 µg/mL Unstressed
Purity 99% +/- 132.7176 µg/mL Stressed

16 Endrin aldehyde 2,015.5 µg/mL +/- 11.8281 µg/mL Gravimetric

CAS # 7421-93-4 (Lot ER082306-01) +/- 91.8045 µg/mL Unstressed
Purity 99% +/- 132.5532 µg/mL Stressed

17 4,4'-DDT 2,016.5 µg/mL +/- 11.8340 µg/mL Gravimetric

CAS # 50-29-3 (Lot S37912V) +/- 91.8500 µg/mL Unstressed
Purity 99% +/- 132.6190 µg/mL Stressed

18 Endosulfan sulfate 2,009.3 µg/mL +/- 11.7914 µg/mL Gravimetric

CAS # 1031-07-8 (Lot BCBT8804) +/- 91.5198 µg/mL Unstressed
Purity 95% +/- 132.1422 µg/mL Stressed

19 Methoxychlor 2,006.5 µg/mL +/- 11.7753 µg/mL Gravimetric

CAS # 72-43-5 (Lot 7322100) +/- 91.3945 µg/mL Unstressed
Purity 99% +/- 131.9613 µg/mL Stressed

20 Endrin ketone 2,007.0 µg/mL +/- 11.7782 µg/mL Gravimetric

CAS # 53494-70-5 (Lot 160405JLM) +/- 91.4173 µg/mL Unstressed
Purity 99% +/- 131.9942 µg/mL Stressed

Solvent: Hexane/Toluene (50:50)

CAS # 110-54-3/108-88-3
Purity 99%

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Column:
30m x .25mm x .2um
Rtx-CLP II (cat.# 11323)

Carrier Gas:
helium-constant pressure 20 psi.

Temp. Program:
150°C to 300°C
@ 4°C/min. ( hold 5 min.)

Inj. Temp:
200°C

Det. Temp:
300°C

Det. Type:
ECD

This chromatogram represents a general set of testing conditions chosen for product
acceptance. For optimal results in your lab, conditions should be adjusted for your
specific instrument, method, and application.

Date Mixed: 03-May-2018 Balance: B442140311

Manufactured under Restek's ISO 9001:2015

Date Passed: 18-May-2018
Registered Quality System
Certificate #FM 80397

Page 4 of 7
General Certified Reference Material Notes

Expiration Notes:
· Expiration date valid for unopened ampul stored in compliance with the recommended conditions.
· Uncertainty, concentration, and expiration of the CRM are based on the unopened product being stored according to the
recommended condition found in the storage field.
Purity Notes:
· Purity and/or chemical identity are determined by one or more of the following techniques: GC/FID, HPLC, GC/µECD,
GC/MS, LC/MS, RI, and/or melting point.
· Compounds with a listed purity of less than 99% have been weight corrected to compensate for impurities and/or salts. A
correction factor is used to calculate the amount of compound necessary to achieve the desired concentration of the
parent compound in solution.
· Purity of isomeric compounds is reported as the sum of the isomers.
· Purity values are rounded to the nearest whole number.
Certified Uncertainty Value Notes:
· The uncertainties are determined in accordance with ISO 17034 and Guide 35. The certified combined stressed
uncertainty value ( includes gravimetric uncertainty, homogeneity between-ampul uncertainty, storage stability
uncertainty and shipping stability uncertainty and were combined using the following formula:

k is a coverage factor of 2, which gives a level of confidence of approximately 95%.

· It is important to note that the shipping stability uncertainty was obtained under temperature extremes for specific time
intervals; therefore, the certified combined stressed uncertainty value should only be applied to the product if it was
stored at non-standard temperature conditions up to and including 7 days. Contact Restek Technical Service at
www.restek.com/Contact-Us for use recommendations if your shipment was in-transit for more than 7 days at non-
standard temperature conditions.
· Apply the certified combined unstressed uncertainty value if the product was received under standard shipping
conditions. Apply the certified combined stressed uncertainty value if the product was received under non-standard
conditions as specified below.

Label Conditions Standard Conditions Non-Standard Conditions

25°C Nominal (Room Temperature) < 60°C ≥ 60°C up to 7 days

10°C or colder (Refrigerate) < 40°C ≥ 40°C up to 7 days
0°C or colder (Freezer) < 25°C ≥ 25°C up to 7 days

· Separate (not combined) uncertainty values for gravimetric uncertainty are also displayed on the certificate, if needed,
separate homogeneity between-ampul uncertainty, storage stability uncertainty and shipping stability uncertainty values
are available by contacting Restek Technical Service at www.restek.com/Contact-Us.
· The packaged amount is the minimum sample size for which uncertainty is valid. The ampules are over-filled to ensure
that the minimum packaged amount can be sufficiently transferred.
Manufacturing Notes:
· Concentration is based upon gravimetric preparation using either a balance whose calibration has been verified daily
using NIST traceable weights, and/or dilutions with Class A glassware.
Handling Notes:
· Stability of the unopened product, when stored in compliance with the recommended conditions, is guaranteed through
the expiration displayed on the product label and certificate. Contact Restek for additional opened product stability
information, with the knowledge/understanding that open product stability is subject to the specific handling and
environmental conditions to which the product is exposed. For your convenience Restek supplies deactivated vials with
most standards packed in 2mL ampules. Larger volume deactivated vials are available through Restek as a custom
ordered item. Additionally, Restek sells DMDCS for the purpose of glassware deactivation as catalog number 31861,
which includes complete instructions.
· If any undissolved material is visible inside the ampul, sonicate the unopened ampul until the material is completely
dissolved.

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 Chemical Standard Batch Sheet
Lot #: A0137734

Catalog #: 32415 Target: 2000 ug/mL

Description: Organochlorine Pesticide Mix AB #3
Solvent: Hexane/Toluene (50:50) Solvent Lot: 0000187959/dt250 Final Volume: 200 ml

Made by: Tom Suckar Date: 5/3/2018 12:51:52PM

Tested by: Fang-Yun Weaver Date: 5/4/2018 7:52:38AM
Pass By: Amanda Miller Date: 5/18/2018 9:29:15AM
Packaged by: Tom Suckar / Brandon Reish Date: 5/3/2018 2:47:59PM No. Units: 128 Pkg Size: 1 mL
Balance Used: BEDEARMBALPC1 XPE205 Serial #: B442140311

Storage Target Calc

Compound CAS Location Lot # Purity Conc(ug/mL) Target Actual Conc(ug/mL)
Endrin 72-20-8 F0067 6898800 0.98 2,000.00 408.16 mg 410.70 mg 2,012.4
alpha-BHC 319-84-6 R0476 0911942 0.99 2,000.00 400.00 mg 403.30 mg 2,016.5
beta-BHC 319-85-7 R0477 BCBS8692V 0.98 2,000.00 408.16 mg 410.10 mg 2,009.5
delta-BHC 319-86-8 R0478 ER02101401 0.99 2,000.00 400.00 mg 403.70 mg 2,018.5
4,4'-DDD 72-54-8 R0481 HAN02-KNCK 0.99 2,000.00 400.00 mg 401.10 mg 2,005.5
4,4'-DDE 72-55-9 R0482 GHYQG 0.99 2,000.00 400.00 mg 402.50 mg 2,012.5
4,4'-DDT 50-29-3 R0483 S37912V 0.99 2,000.00 400.00 mg 403.30 mg 2,016.5
Aldrin 309-00-2 R0498 7321900 0.99 2,000.00 400.00 mg 402.90 mg 2,014.5
Dieldrin 60-57-1 R0509 5709400 0.98 2,000.00 408.16 mg 408.00 mg 1,999.2
Endosulfan I 959-98-8 R0511 ER012105-02 0.99 2,000.00 400.00 mg 403.20 mg 2,016.0
Endosulfan II 33213-65-9 R0512 7276300 0.99 2,000.00 400.00 mg 403.60 mg 2,018.0
Endosulfan sulfate 1031-07-8 R0513 BCBT8804 0.95 2,000.00 421.05 mg 423.00 mg 2,009.3
Heptachlor 76-44-8 R0516 NT060133 0.99 2,000.00 400.00 mg 403.00 mg 2,015.0
gamma-BHC (Lindane) 58-89-9 R0522 6815700 0.99 2,000.00 400.00 mg 403.70 mg 2,018.5
Methoxychlor 72-43-5 R0523 7322100 0.99 2,000.00 400.00 mg 401.30 mg 2,006.5
Heptachlor epoxide (isomer B) 1024-57-3 R0538 ER121511-01 0.99 2,000.00 400.00 mg 401.50 mg 2,007.5
Endrin ketone 53494-70-5 R0634 160405JLM 0.99 2,000.00 400.00 mg 401.40 mg 2,007.0
trans-Chlordane 5103-74-2 R0643 ER061906-04 0.99 2,000.00 400.00 mg 401.80 mg 2,009.0
cis-Chlordane 5103-71-9 R0644 ER062410-01 0.99 2,000.00 400.00 mg 401.60 mg 2,008.0
Endrin aldehyde 7421-93-4 R0724 ER082306-01 0.99 2,000.00 400.00 mg 403.10 mg 2,015.5

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 QA Report: Organochlorine Pesticide Mix AB #3 (Cat#32415)
Runs of Lot # A0108854 32415.sec Runs of Lot # A0137734
COMPONENT Run #1 Run #2 Run #3 AVG STD DEV % RSD Run #1 Run #2 Run #3 AVG STD DEV % RSD %D MEAN P/F
alpha-BHC 2446168887 2487885549 2595245885 2509766774 76909550 3.06 2463308563 2554183209 2512668888 2510053553 45493739 1.81 -0.01 PASS
gamma-BHC (Lindane) 2188176113 2227629543 2322493779 2246099812 69037468 3.07 2217822724 2296655476 2259091806 2257856669 39430887 1.75 -0.52 PASS
beta-BHC 805472257 819172169 854875126 826506517 25505007 3.09 814894214 842944572 830025483 829288090 14039710 1.69 -0.34 PASS
delta-BHC 2291793316 2328517689 2432878685 2351063230 73194921 3.11 2342084098 2426528039 2384960039 2384524059 42223659 1.77 -1.42 PASS
Heptachlor 1797498504 1870959184 1973670116 1880709268 88489589 4.71 1974546208 2042948028 2017452449 2011648895 34568239 1.72 -6.96 PASS
Aldrin 2076330657 2107190937 2191378506 2124966700 59548175 2.80 2046483777 2113244563 2080405861 2080044734 33381858 1.60 2.11 PASS
Heptachlor epoxide (isomer B) 1805123318 1827640543 1901589678 1844784513 50466587 2.74 1816832944 1876192351 1845643916 1846223070 29683941 1.61 -0.08 PASS
trans-Chlordane 1969883472 1997717002 2080281016 2015960497 57415357 2.85 1971650246 2037916598 2003050476 2004205773 33148279 1.65 0.58 PASS
cis-Chlordane 1877653504 1904660262 1983992691 1922102152 55273595 2.88 1887915366 1952891536 1918831344 1919879415 32500762 1.69 0.12 PASS
Endosulfan I 1718342011 1739847317 1810403137 1756197488 48159203 2.74 1691493514 1747997032 1718214281 1719234942 28265583 1.64 2.10 PASS
4,4'-DDE 1874416284 1901128684 1980504969 1918683312 55179942 2.88 1896702017 1961070357 1928590634 1928787669 32184622 1.67 -0.53 PASS
Dieldrin 1962119701 1987111881 2069848987 2006360190 56385041 2.81 1981004263 2048659864 2014972997 2014879041 33827898 1.68 -0.42 PASS
Endrin 1483348266 1517372089 1591516925 1530745760 55310542 3.61 1573186356 1632629430 1622787471 1609534419 31860670 1.98 -5.15 PASS
4,4'-DDD 1567891495 1598591028 1672519802 1613000775 53781979 3.33 1608392986 1663772830 1640192656 1637452824 27791398 1.70 -1.52 PASS
Endosulfan II 1630100544 1647618682 1716965388 1664894871 45937189 2.76 1495551112 1543021126 1520642654 1519738297 23747925 1.56 8.72 PASS
Endrin aldehyde 1580406046 1632313103 1723574696 1645431282 72480208 4.40 1670689531 1728991223 1705153113 1701611289 29311776 1.72 -3.41 PASS
4,4'-DDT 1325356618 1337895341 1393478419 1352243459 36256673 2.68 1341767527 1381659567 1361289404 1361572166 19947523 1.47 -0.69 PASS
Endosulfan sulfate 1532859666 1549005418 1612819186 1564894757 42281610 2.70 1602741477 1654453569 1630341323 1629178790 25875640 1.59 -4.11 PASS
Methoxychlor 638495515 667613023 704180178 670096239 32912665 4.91 709309101 735962820 729702594 724991505 13937394 1.92 -8.19 PASS
Endrin ketone 1786785542 1811265908 1890613094 1829554848 54276184 2.97 1815463671 1877827337 1845615712 1846302240 31187501 1.69 -0.92 PASS

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