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  • QC - Qubit - Amended For COG-train

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    Carlos Ramírez
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    The document discusses quality control using the Qubit system. The Qubit uses fluorescent dyes that bind specifically to DNA or RNA and emit light when excited, allowing quantification of nucleic acid concentration based on signal strength. It provides sensitive and accurate measurement of DNA or RNA concentration over various ranges, from 2-1000 ng for DNA broad range down to 0.2-100 ng for high sensitivity DNA, and is well suited for applications like PCR and NGS, though it does not provide information on purity or integrity of the nucleic acids.

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    QC_-_Qubit_-_amended_for_COG-train.pptx

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    The document discusses quality control using the Qubit system. The Qubit uses fluorescent dyes that bind specifically to DNA or RNA and emit light when excited, allowing quantification of nucleic acid concentration based on signal strength. It provides sensitive and accurate measurement of DNA or RNA concentration over various ranges, from 2-1000 ng for DNA broad range down to 0.2-100 ng for high sensitivity DNA, and is well suited for applications like PCR and NGS, though it does not provide information on purity or integrity of the nucleic acids.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    14 views4 pages

    QC - Qubit - Amended For COG-train

    Uploaded by

    Carlos Ramírez
    The document discusses quality control using the Qubit system. The Qubit uses fluorescent dyes that bind specifically to DNA or RNA and emit light when excited, allowing quantification of nucleic acid concentration based on signal strength. It provides sensitive and accurate measurement of DNA or RNA concentration over various ranges, from 2-1000 ng for DNA broad range down to 0.2-100 ng for high sensitivity DNA, and is well suited for applications like PCR and NGS, though it does not provide information on purity or integrity of the nucleic acids.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    of 4

    Quality Control

    Qubit
    Dr Paola Niola FHEA
    Senior Applications Specialist
    Teaching Fellow
    UCL Genomics
    Learning outcomes

    You will learn:

    1. How the Qubit works


    QUBIT
    • Fluorescent dye-based quantitation method

    • Fluorescent dye binds specifically DNA or RNA:


    when excited 🡪 light emission

    • Higher the signal is, higher the concentration is!


    QUBIT
    DNA
    or R
    NA

    DNA Broad 2–1,000 ng


    Range
    HS DNA 0.2–100 ng

    RNA Broad 20–1,000 ng


    Range
    HS RNA 5–100 ng

    ng/µL

    • More sensitive and accurate • No information about purity


    • Good for PCR, NGS • No information about integrity

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