Highly Confidential Lab Result

Patient Name and surname : Mrs. CHANDRA KALA Nationality : INDIAN

ID or Passport No. : 277798873021 Bill to : CASH

Date Of Birth : 1948 Age / Gender : 73 years / Female

Cell No : 9435107800 Medical AID :-

Referral Practitioner : SELF Sample Collection Date & Time : Aug 07, 2021, 09:13 a.m.
SRF ID :- Resulted date & Time : Aug 07, 2021, 14:16 p.m.

Sample ID :
422031

Investigations Result(s)

COVID-19 RT-PCR
(Nasopharyngeal or Oropharyngeal Swab)

Truenat Beta CoV (E gene)

Not Detected
Method : Screening

Truenat SARS CoV-2 (Orf 1 A gene)

Not Detected
Method : Confirmation

Result Negative

ICMR Lab ID: PHDIGUA Principle : Truenat tests works on principle of Real Time Reverse Transcription Polymerase Ch ain Reaction (RT-PCR). It
involves the specific amplification of target region. This analysis is done on Truelab real time PCRby using the sensitive an d specific Taqman assay
method. Amplified products are indicated by threshold cycle (CT) in amplification curve. Assay result should be interpreted only in the context of
other laboratory findings and the total clinical status of patient. Pathogen Information : Viruses belonging to the family of beta corona virus
(Sarbeco) is the causative agent for SARS (Severe Acute Respiratory Syndrome) and COVID-19. Truenat Beta CoV is first line screening test &
detects both SARS and Covid-19 causative viruses. Analytical Sensitivity : The clinical sensitivity, Clinical specificity and overall concordance were
determined to be 100%.The Performance of this test is been Validated and Evaluated by ICMR & has been recommended by ICMR for Screening &
Confirmation of COVID-19. Target Selection : The target sequence used for Truenat Beta CoV is E gene of Sarbeco virus, and that f or Truenat
SARS CoV-2 is Orf1a gene. Procedural Limitations: 1. Mutations within the highly conserved regions of the target genome may result in the
under-quantitation of or a failure to detect the presence of the concerned pathogen. 2. A number of factors could lead to variation in test results
including poor quality of specimen, low sample volume, timing of sample collection( late or very early in the course of infec tion), sample collection
and transportation, technical reasons like viral mutations. The test result must always be evaluated with other clinical data. 3. The CT values do not
provide a measure of viral load due to inherent variability in sampling and kits. According to ICMR guidelines, CT values should not be used to
gauge the potential risk of infection.

Scan to Validate