H H

F-XC A N GE F-XC A N GE
PD PD

!
W

W
O

O
N

N
y

y
bu

bu
to

to
Laboratorium TUK SMK-SMAK Bogor - HPLC Hitachi Chromaster
ww

ww
om

om
k

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lic

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C

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tr re tr re
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ac ac
k e r- s o ft w a k e r- s o ft w a

PUSAT PENDIDIKAN DAN PELATIHAN INDUSTRI

SEKOLAH MENENGAH KEJURUAN - SMAK BOGOR
LABORATORIUM TEMPAT UJI KOMPETENSI (TUK) SMAKBO

LAPORAN HASIL ANALISIS HPLC CHROMASTER

Nama : Standar thiamin 10 ppm

Data File : C:\Users\HP\Desktop\A1&A4 13-6\Validasi Metode Sampel Vitamin B1Kelompok
B1&B.rslt\Cal_Vitamin B1002.dat
Method : C:\Users\HP\Desktop\B1&B4 13.6\Kelompok B1&B4.met
Acquired : 10/5/2021 2:55:08 PM (GMT +07:00)
Printed : 10/6/2021 12:14:43 PM (GMT +07:00)

DAD-CH1 254 nm
Cal_Vitamin B1002
Retention Time

100 100
mAU

mAU
50 50

2.820

3.047
0.173

0.367
0.447
0.560

0.747

0.940

1.140

1.320
1.413
1.520

1.713

1.900

2.307

3.253

3.440

3.640

3.833
2.667

0 0

0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0

Minutes

DAD-CH1 254
nm Results
Retention Time Area Area % Height Height %
0.173 8575 0.17 1093 0.14
0.367 29591 0.58 2912 0.38
0.447 18308 0.36 3645 0.48
0.560 28040 0.55 4723 0.62
0.747 75821 1.49 6482 0.85
0.940 103589 2.03 8308 1.09
1.140 94621 1.86 10202 1.34
1.320 134065 2.63 11926 1.57
1.413 66766 1.31 12760 1.68
1.520 88007 1.73 13777 1.82
1.713 175945 3.45 15606 2.06
1.900 197354 3.87 17378 2.29
2.307 492330 9.65 21447 2.83
2.667 2734322 53.61 552872 72.84
2.820 337697 6.62 25739 3.39
3.047 214935 4.21 18953 2.50
3.253 143697 2.82 14202 1.87
 H H
F-XC A N GE F-XC A N GE
PD PD

!
W

W
O

O
N

N
y

y
bu

bu
to

to
Laboratorium TUK SMK-SMAK Bogor - HPLC Hitachi Chromaster
ww

ww
om

om
k

k
lic

lic
C

C
.c

.c
w

w
tr re 3.440 94518 1.85 10030 1.32 tr re
.

.
ac ac
k e r- s o ft w a k e r- s o ft w a
3.640 52761 1.03 5643 0.74
3.833 9596 0.19 1354 0.18

Totals
5100538 100.00 759052 100.00