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a sciencedirect.com
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f Activity and interactions of
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n levofloxacin, linezolid, ethambutol and
d
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amikacin in three-drug combinations
m against Mycobacterium tuberculosis
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n isolates in a human macrophage
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model
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d Emma Rey-Jurado, Griselda Tudó, Dolors Soy, Julian
e González-Martín
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o International journal of antimicrobial agents 42 (6),
n 524-530, 2013
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o Multidrug resistance is a problem in the management
l of tuberculosis, creating an urgent need for new
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regimens including currently available drugs.
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Macrophage models allow an evaluation of the effect
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ç
of drugs against intracellular bacilli. The effect of the
ã following different drug combinations against six
o multidrug-resistant and six drug-susceptible clinical
d isolates of Mycobacterium tuberculosis multiplying
o inside the human macrophage THP-1 cell line was
e studied: levofloxacin/linezolid/ethambutol;
s levofloxacin/amikacin/ethambutol; and
p
levofloxacin/linezolid/amikacin. Macrophages were
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ç
lysed, seeded onto Middlebrook 7H11 plates and
o CFU were counted after 21 days of incubation. The
p interaction of the drugs in combination was
e interpreted by the effect of the combination compared
d with the most active single drug alone. The
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