ABS Operating Manual Class II - RG - PM (Old)

Caution:
Please read this Manual thoroughly.

If you do not understand or are unsure of any
information contained in this Manual please
contact your Agent or Astec Microflow for
clarification.
OPERATING MANUAL
MICROFLOW
ADVANCED BIO SAFETY CABINET
(ABS)
CLASS II
part of BIOQUELL (UK) Limited

34 Walworth Road
Andover
Hants
SP10 5AA
Telephone: + 44 (0) 1264 835835
Fax: + 44 (0) 1264 836836
Ref: RG/PM/04/01
CONTENTS
Warranty Information
Foreword
Safety Environmental Conditions
1 Introduction
2 Specification
2.1 Operating Environment
2.2 Construction
2.3 Electrical
2.4 Alarm
2.5 Lighting
2.6 Filtration
2.7 GS Approval
3 General Instructions
3.1 Control Panel
3.2 Additional Features
3.3 Front Sliding Window
3.4 Work Surface
3.5 Front Sliding Window Limit Switches
3.6 Solenoid Operated Gas Inlet
3.7 Mains Socket
3.8 Services Tap
3.9 Cabinet Mains Power Connection
3.10 Plenum Sample Port
3.11 Exhaust Filter Sample Port
4 Optional Extras
4.1 Formalin Vaporiser
4.2 Ultra Violet (UV) Light
4.3 Fireboy Gas Burner
4.4 Touch-o-matic Gas Burner
4.5 Hot Wire Anemometer
4.6 Base Assembly
4.7 Drain Tray
4.8 Volt Free Contact
4.9 Internal Socket Residual Current Device
4.10 Exhaust Kits
5 Installation
5.1 Preparation for Use
6 Decontamination of the Cabinet

6.1 Swabbing Down
6.2 Fumigation
6.3 Gassing the Laboratory
7 Maintenance
7.1 Daily/Frequently
7.2 Weekly/Monthly
7.3 Main and Exhaust Filter Servicing
7.4 Fluorescent Tube Replacement
7.5 UV Tube Replacement
7.6 Circuit Protection
8 Control System: Customisation and Calibration
9 Calibration of Airflow and Alarms
10 Additional Airflow Set Up Functions
11 Spares List
12 Servicing Record
Appendices
1 Notes on COSHH Regulations (UK Only)
2 UK Approved Service Companies Telephone and Fax Numbers
3 Care and Cleaning
4 Automatic Fumigation
5 Model and Specification Data
6 Safety Symbols
7 Protocol for Testing, Monitoring, Replacement and Disposal of Filters
8 Installation Guidance
9 EC Declaration of Conformity and Certificate of Conformity
10 Technical Bulletins
11 Test Equipment
Important, please read this manual before
using this equipment.
Thank you for purchasing our Astec Microflow product. We are confident that you
will be completely satisfied with its design, function and quality.
Export and Agent Sales Only
Your warranty arrangements will be with your Agent. Please confirm the warranty
directly with your Agent.
UK Sales Only
To register the product for our warranty you can either copy this page and fax the
details below to us on 01934 410546 or e-mail sales@astec-microflow.co.uk. Full
conditions of our warranty and how to arrange a warranty visit are on the next three
pages.
The units are calibrated for airflow before leaving the factory however trained
personnel can recalibrate them on site.
If you require any help, advice, or have any problems please contact us on our
Technical Help-Line 0800 716107. If a warranty visit is required please complete the
Warranty Visit Request Fax and send to us on 01934 410546.
WARRANTY REGISTRATION FAX

HOSPITAL / COMPANY
CONTACT
TELEPHONE NUMBER
AND EXTENSION
ADDRESS
EQUIPMENT
MODEL
SERIAL NUMBER
LOCATION
PLEASE TICK IF YOU REQUIRE DETAILS OF OUR
SERVICE RECOMMENDATIONS AND PRICES
Warranty and Liability
Astec Microflow produces products that are warranted under normal usage against
defects in workmanship and materials for one-year parts and labour costs, from the
date of manufacture. The Warranty is stated in the Standard Terms and Conditions
of sale.
Export and Agent retailed products are warranted directly by the Agent.
Please confirm your warranty and liability status with the Agent.
In addition, the Warranty is void unless the following conditions are met:
(a) The Warranty Registration Fax has been returned to Astec Microflow UK.
The Warranty Registration Fax is located on the previous page.
(b) The product has been installed and used as stated within the Instruction
Manual.
(c) The warranty does NOT include servicing or maintenance. An approved
service company who have attended our training courses for your product
must carry out maintenance of product. Failure to maintain or service this
product will invalidate the warranty. Maintenance must be carried out in
accordance with the Service Manual and include tasks within stated periods.
Failure to use approved service companies or Astec Microflow trained
personnel for maintenance also affects the CE Marking status of the product,
removing Astec Microflow’s Duty of Care and responsibility under the
following European Directives:
(i) Medical Device **
(ii) EMC
(iii) LVD
(d) Supplies used with the product, such as cleaning solutions **, disinfectants **,
are not covered by this Warranty.
(e) Consumables such as carbon filters, pre-filters, HEPA filters, light bulbs and
tubes, not warranted.
(f) This Warranty is void if faults are caused by accidental damage, mishandling,
adjustment by unauthorised personnel or failure to follow the correct
maintenance and safety precautions as stated in the Instruction Manual.
(g) The Warranty expressly provided for herein is the sole Warranty provided in
connection with the product and no other Warranty, expressed or implied, is
provided. Astec Microflow assumes no responsibility for any other claims,
consequential (including lost time or profit) or other damage, whether based in
contract, tort or otherwise, not specifically stated in this Warranty.
(h) Except in respect of death or personal injury caused by Seller’s negligence, or
as expressly provided in these Conditions, Seller shall not be liable to Buyer
by reason of any representation (unless fraudulent), or any implied warranty,
condition or other term, or any duty at common law, or under the express
terms of the Contract for any loss of profit or any indirect, special or
consequential loss, damage, costs, expenses or other claims (whether
caused by the negligence of Seller, its servants or agents or otherwise) which
arise out of or in connection with the supply of the Goods or their use or
resale by Buyer, and the entire liability of Seller under or in connection with
the Contract shall not exceed the price of the Goods.
Note:
When requesting a Warranty visit, please have the following information
available:
(i) Product model number and name.
(ii) Serial number.
(iii) Date of last service, and Service Company.
(iv) Nature of fault and any other comments likely to indicate cause of fault.
(v) A Purchase Order number to cover costs incurred if visit is outside the
scope of the Warranty.
Astec Microflow, or our sister company BIOQUELL Service, or other
nominated personnel will carry out warranty visits.
(j) In the event of any health and safety incidents please advise us in writing at
the earliest opportunity.
(k) This warranty and all other contractual issues shall be governed by English
law and the parties agree to submit to the nonexclusive jurisdiction of the
courts of England.
Items marked ** are relevant to our Safescope models

Please copy before use.
WARRANTY VISIT REQUEST FAX

Customer name
…………………………………………………………………………………………….……
Location of product including address and contact telephone number

…………………………………………………………………………………………………
…………………………………………………………………………………………………
…………………………………………………………………………………………………
…………………………………………………………………………………………………
…………………………………………………………………………………………………
Product model number and name
………………………………………………………………………………………………….
Serial number (on front or right hand side on unit)
………………………………………………………………………………………………….
Date of last service, and Service Company
………………………………………………………………………………………………….
Nature of fault and any other comments likely to indicate cause of fault
…………………………………………………………………………………………………
…………………………………………………………………………………………………
…………………………………………………………………………………………………
…………………………………………………………………………………………………
…………………………………………………………………………………………………
…………………………………………………………………………………………………
…………………………………………………………………………………………………
…………………………………………………………………………………………………
…………………………………………………………………………………………………
…………………………………………………………………………………………………
…………………………………………………………………………………………………
…………………………………………………………………………………………………
…………………………………………………………………………………………………
…………………………………………………………………………………………………
…………………………………………………………………………………………………
…………………………………………………………………………………………………
…………………………………………………………………………………………………
…………………………………………………………………………………………………
…………………………………………………………………………………………………
Purchase Order number to cover costs incurred if visit is outside the scope of the
Warranty ………………………………………………………………………..…………….
Export Only UK Only

Please fax to your product Agent. Please fax to 01934 410546.
FOREWORD
This manual has been specially prepared to give guidance in the use and
maintenance of the:
MICROFLOW
LAMINAR FLOW
ADVANCED BIO SAFETY CABINET (ABS)
CLASS II
(specific variants comply with the requirements of EN 12469 and are ‘GS’ marked)
WARNINGS:
(1) USE OF THE APPLIANCE IN A WAY OTHER THAN DESCRIBED IN THIS
MANUAL COULD PROVE TO BE VERY DANGEROUS.
(2) GERMICIDAL LAMP. EMITS UV RADIATION 253.7 nm. EXPOSURE WILL

CAUSE DAMAGE TO SKIN AND EYES. VACATE AREA WHEN IN USE.
In order to utilise the full operator and product protection factors given by the cabinet,
it is essential that the operator is fully familiar with the cabinet and all of its functions
and controls. This manual should be thoroughly studied before the cabinet is used.
This manual is supplied with both stainless steel and polypropylene lined versions.
Care must be taken in the treatment of both linings, see Appendix 3.
This manual contains the operational and first line maintenance instructions. Service
and maintenance operations other than those covered by this manual should only be
undertaken by Astec Microflow’s recommended service agent, see Appendix 2.
Care should be taken to follow the instructions in this manual to ensure safe
operation. However, if further clarification is required contact your supplier.
SAFETY ENVIRONMENTAL CONDITIONS
The cabinet is for indoor use only.
For use at altitudes up to 2000 m.
Operating temperature range of 10°C to 35°C.
For operation at a maximum relative humidity of 80% for temperatures up to 31°C,

decreasing linearly to 50% relative humidity at 40°C.
Installation category (overvoltage category) II.
Pollution degree 1.
Microflow is a registered trademark of BIOQUELL plc. Microsoft (MS) is a registered

trademark of Microsoft Corporation. Windows™ is a trademark of Microsoft
Corporation.
1 INTRODUCTION
The use of laminar flow techniques to provide a clean sterile environment

and operator protection has long been established and is embodied in
standards issued by the US National Institute of Health (NIH) and the British
Standards Institute.
The bench mounted laminar flow biological safety cabinets have been
developed to provide operator protection similar to a Class I cabinet and for
the work, as laid down by NIH, BS 5726 and EN 12469. However, the
degree of hazard related to each particular laboratory procedure should be
determined to confirm that the protection factors are satisfactory.
The cabinets work on a recirculating principle, approximately 70% of the air

drawn from the working area being recirculated. Air then passes through the
main filter mounted in the roof of the working area, at a velocity in the region
of 0.4 m/sec dependent on the standard required. The unit is self balancing,
drawing its make up air through the open front and discharging it through a
high efficiency exhaust filter.
The fan system has been designed so that no external part of the main
cabinet is under positive pressure to the laboratory thus eliminating the need
to continually leak test the cabinet structure.
Figure 1 Laminar Flow Advanced Bio Safety Cabinet

2 SPECIFICATION
2.1 OPERATING ENVIRONMENT
The cabinet will operate satisfactorily in environments where the ambient

temperature lies between 10 and 35°C and the relative humidity does not
rise above 80%. Condensation of moisture on the cabinet must be avoided
at all times, as this will affect filter efficiency.
2.2 CONSTRUCTION
The outer shell is of mild steel sheet finished in white powder coating. The
work area is made from 316 stainless steel or polypropylene (optional) liners.
Special attention has been paid in the design and construction to avoid
crevices. The Front Sliding Window uses laminated glass.
2.3 ELECTRICAL
The ABS cabinet has two power supply options:

(a) 230 V ±10%, 50 Hz single phase supply.
(b) 110 V ±5%, 60 Hz single phase supply.
The internal control system operates at 5 V.
2.4 ALARM
Safety has been considered the primary factor in the design of this cabinet.
Consistent with this, the display will indicate alarm conditions which include
the following parameters:
(a) Downflow Velocity out of specification (high and low limits settable).
(b) Inflow Velocity Low.
(c) Main Filter Dirty.
(d) Front Sliding Window not secure (in either raised or lowered position).
Activation of any alarm causes an audible and visual signal.
2.5 LIGHTING
Fluorescent tubes provide a lighting level in excess of 800 Lux over most of
the working area.
2.6 FILTRATION
High efficiency filtration is provided by Astec Microflow Narrow Pleated

HEPA filters giving an efficiency of not less than 99.997% (BS 3928).
2.7 ‘GS’ APPROVAL
The cabinet has achieved approval to EN 12469. Validation was carried out
by TÜV Product Service GmbH and the cabinet therefore carries the ‘GS’
mark. The approval applies only to the units purchased to EN 12469 and
when used in recirculatory mode. This approval covers the standard
cabinet. Units installed and used outside of these conditions are not
conforming to the TÜV approval.
3 GENERAL INSTRUCTIONS
3.1 CONTROL PANEL
The touch controls are located on the Control Panel (Fig. 2) and are
described in the following paragraphs.
Figure 2 Control Panel
3.1.1 Mains Power Indicator
The blue indicator is lit when the mains supply has been
connected to the cabinet. The indicator remains lit at all times.
3.1.2 Alarm indicator
The alarm indicator flashes whenever an alarm condition occurs.

Additionally, unless muted by pressing the MUTE button, the
buzzer emits an alternating two tone signal to give warning of an
alarm condition.
3.1.3 Fan Control
A special requirement to meet the requirements of the ‘GS’

approval is that access to this function is protected to prevent
unauthorised use. Thus for the EN 12469 purchased variant,
pressing this control (for on and off) will prompt the user to enter a
(configurable) four digit PIN code. Only after acceptance of this code will the
desired change in operating state of the unit be actioned. Entry of an
incorrect code, or failure to start to enter a code within 14 seconds will cause
no change in operating condition of the cabinet.
Operation of this switch causes the cabinets internal and external fan(s)
(where fitted) together with the airflow control system to operate. Alarms are
muted for a period of about 1 minute during which time the display will show
the message ‘STABILISING FLOW’. If after this time no alarms are active
the correct airflow will have been established and the display will show an
appropriate message, for example ‘CLASS II CABINET SAFE’.
A second press of the fan button causes the fan(s) to be switched off.
Alternatively, one of the other fan controls, or the vaporisation cycle can be
selected to provide the desired action.
3.1.4 Fan Off Delay

Pressing this button when the fan has been running (LEDs of buttons with
numbers (1) or (3) lit) will cause the fan to be switched off after expiry of a
timed period.
The delay period is configurable from the set-up software. See Section 8
‘Control System: Customisation and Calibration’.
Pressing this button a second time cancels the remaining delay time and
switches off the fan(s).
Once the fan delay is activated, it can alternatively be canceled by pressing

either of the other two fan functions: buttons (1) or (3). This will initiate the
relevant function
3.1.5 Fan 100%

Pressing this button will cause the cabinet’s internal fans to operate at full
speed. This facility is intended for emergency use after a spillage or other
inadvertent activity. If an exhaust fan is fitted, this will continue to run at its
preset speed.
A second press of this button will switch the cabinet’s internal and exhaust
fans off. Alternatively, either of the other two fan buttons can be pressed in
order to select the desired action.
3.1.6 Alarm Mute
When an alarm condition occurs, the buzzer will sound. Pressing

this button will cause the buzzer to be muted for a configurable
time period of between 0 and 255 minutes. See Section 8
‘Control System: Customisation and Calibration’. The default setting is 4
minutes. Cabinets to EN 12469 require the default setting at 0 minutes.
The intermittent beeping that accompanies: ‘FLOW STABILISATION’,
‘EXPIRED SERVICE DATE’ messages, or attempts to select incompatible
conditions are not muted.
3.1.7 Test
Correct operation of switch indicators and the audible alarm may

be checked by pressing the ‘TEST’ button; all indicators should
light and the buzzer should sound with a continuous tone.
The ‘TEST’ function serves the secondary purpose of allowing the ‘HOURS
RUN’ and ‘SERVICE DATE DUE’ information to be displayed. This data is
useful in the planning of maintenance operations.
3.1.8 Fluorescent Lights
Pressing this switch turns on the cabinet’s fluorescent lighting.

With power connected to the cabinet, the lights can be switched
on at any time.
If UV lamp is in use or the UV lamp switch has been pressed (UV lamp
switch LED lit), it will automatically be switched off when the fluorescent light
is selected.
3.1.9 UV Lamp
WARNING:
DUE TO ITS HAZARDOUS EFFECTS, THE CABINET
OPERATOR SHOULD AVOID ANY EXPOSURE TO UV
RADIATION.
When selected, the UV lamp will remain on for a configurable time period
after which it will automatically switch off. The default setting is 15 minutes.
See Section 4.2 ‘Ultra Violet Light’ for details of operation and safety
interlocks.
The time period for which the UV lamp remains on can be changed, see
Section 8 ‘Control System: Customisation and Calibration’.
3.1.10 Vaporisation Cycle
Pressing this switch initiates the fumigation process. See Section

6.2 ‘Fumigation’ for the fumigation procedure and Appendix 4 for
the automatic procedure.
3.1.11 Gas Supply
As a safety feature, in series with the gas supply tap is a solenoid

valve which is controlled from the control panel. Thus, the gas
supply can only be selected if the airflow is in a safe condition (i.e.
no alarms are active). Once the valve has opened, gas supply can be
controlled via the tap inside the cabinet in the usual way.
Caution:
The solenoid valve will automatically close the gas supply, if an alarm occurs
during the time it is selected.
3.1.12 Keylock and PIN Number Change
Keylock
The keylock switch offers different operability depending on the

cabinet model number involved.
The fan control lock functions is compulsorily built into the standard
operation of the fan switches. Thus, the functionality of this key reduces to
the provision of allowing the user to modify the existing PIN codes for the fan
and vaporiser functions.
PIN Number Change
Pressing the key followed by entering the appropriate code will prompt the
user to enter a new four digit number.
3.2 ADDITIONAL FEATURES
3.2.1 Automatic Air Velocity Control
The control system automatically compensates the airflow for the dirtying of
filters and variations in the mains power supply. Whenever the airflow is
within safe operating conditions, the display reports the message ‘CLASS II
CABINET SAFE’.
If an alarm condition is detected, the buzzer will sound and the alarm LED
will flash; the cause of the alarm will be reported on the display.
After switching on the cabinet from cold, an airflow alarm may occur for a
short period. This is due to the stabilisation of flow sensors and airflows.
During stabilisation, the display shows the message ‘STABILISING FLOW’.
3.2.2 Automatic Fan Start After Power Failure
In the event of power disconnection from the cabinet while the fan is
operating, reconnection of the power will cause the fan to start. However, all
other functions will be automatically set to off and, if required, they must be
reselected.
3.2.3 Set-up Software
Refer to the Supplier or the Maintenance Provider.
3.2.4 Hour Meter and Service Date Due
These parameters can be viewed on the display by pressing the ‘TEST’

button.
3.3 FRONT SLIDING WINDOW
Using the assistance of a counterbalance, the Front Sliding Window slides

downwards from the operating height to form a closing window for fumigation
(Fig. 3), and upwards to allow access for loading. Care should be taken to
ensure that the window is properly latched at either side.
The Front Sliding Window may also be hinged (Fig. 4), allowing access into
the cabinet for cleaning the inside surface of the window.
To open/move the Front Sliding Window proceed as follows:

Release the door frame by releasing both handles on the hinged door frame.
The frame will move 20 – 30 mm outwards.
With both sides released the glass window can be moved using the handles.
When the window is in position the door frame can be secured by tightening
both handles on the hinged door frame.
Caution:
Care must be taken to avoid impact damage to the bottom edge of the Front
Sliding Window as cracking of the glass may result.
Figure 3 Front Sliding Window - Closed
Figure 4 Front Sliding Window - Hinged
3.4 WORK SURFACE
The actual working surface is lower than its edges so that minor spillages will
be contained. The work surface can be easily removed for cleaning. The
effective limits of the working space are defined by the flat bottom surface of
the work tray.
Caution:
The angled grille in front of the work surface must not be obstructed or the
inflow velocity and operator protection will be reduced. On EN 12469
specified cabinets the optional arm rests should be fitted.
3.5 FRONT SLIDING WINDOW LIMIT SWITCHES
The Limit Switches are magnetic switches (Fig. 5) which detect the position
of the Front Sliding Window and, if closed, de-energise the cabinet’s internal
fans and optional exhaust fan. Further Limit Switches detect that the Front
Sliding Window frame is securely latched closed.
Limit Switches
Figure 5 Front Sliding Window Limit Switch
3.6 SOLENOID OPERATED GAS INLET
Gases for use inside the cabinet are supplied via a solenoid valve (located
behind the side panel) which permits gas to flow only when the cabinet fans
are operating and airflow levels are within the specified parameters. Switch
on the gas supply by pressing the button marked ‘GAS’ on the Control
Panel. The gas flow can then be controlled at the tap (far right in Fig. 6).
Figure 6 Services Taps and Mains Sockets
3.7 MAINS SOCKET
A single mains socket is (2nd from left) is normally fitted to the side wall of the
cabinet. Equipment in excess of 300 W should not be connected to this
socket. If this value is exceeded, the circuit breaker will trip. The circuit
breaker is located on the top right of the cabinet adjacent to the mains
connection socket and on/off switch (far left in Fig. 7).
Electrical safety test on the electrical socket has a maximum permitted earth
leakage of 0.5 mA.
3.8 SERVICES TAP
In addition to the solenoid operated inlet, a second tap without an interlock

valve is provided (2nd from right in Fig. 6). This valve can be used to connect
air vacuum or non-hazardous gas supplies to the cabinet.
3.9 CABINET MAINS POWER CONNECTION
Mains power connection to the cabinet is made by the use of a standard IEC
3 pin connector assembly incorporating dual pole fusing and an isolator
switch (Fig. 7).
Figure 7 Mains and Services
3.10 PLENUM SAMPLE PORT
The plenum is the central area between both filters. A sample port, labeled
‘A’ on the top right hand side of the cabinet, is provided for taking air
samples from the filter plenum. This is used for a measurement of challenge
aerosol or for the measurement of filter pressure drop.
Connection to the port is made by depressing the outermost (locking) ring for
the port labeled ‘A’ and then pulling the red blanking plug from the fitting. A
nylon tube of outside diameter 8 mm maximum can then be pushed in the
hole to make a connection. After testing is completed remove the tube and
refit the blanking plug.
Caution:
The sample port is under positive pressure and also potentially
contaminated. Suitable precautions should be taken.
3.11 EXHAUST FILTER SAMPLE PORT
This port is provided to ease access for testing of the exhaust filter,
particularly in cabinets with top boxes/ducting etc.
A sample port labeled ‘B’ is located adjacent to port ‘A’ on the top right hand
side of the cabinet.
Connection to the port is made by depressing the outermost (locking) ring for
the port labeled ‘B’ and then pulling the red blanking plug from the fitting. A
nylon tube of outside diameter 8 mm maximum can then be pushed in the
hole to make a connection. After testing is completed remove the tube and
the refit blanking plug.
The zone sampled could be under positive or negative pressure (generally

positive pressure with secondary exhaust filters and negative with ducted),
so this should be considered when connection is made. The port is useful
during setting up of the cabinet after a filters change or for checking the
integrity of the primary exhaust filter.
Caution:
The sample port may be under positive pressure and also potentially
contaminated. Suitable precautions should be taken.
4 OPTIONAL EXTRAS
4.1 FORMALIN VAPORISER
A Formalin Vaporiser (Fig. 8) can be fitted to the cabinet to provide a

convenient method for decontamination. The vaporiser can function as part
of the integrated fumigation cycle. Refer to Section 6.2 ‘Fumigation’ for
details on procedures.
Figure 8 Formalin Vaporiser
4.2 ULTRAVIOLET (UV) LIGHT
The UV light can be used for internal cabinet disinfection. The use of UV
light for disinfection purposes is not recommended by BS 5726. However, if
it is used, the bactericidal ultra violet output should be tested frequently as
the germicidal properties may reduce after a limited period even with visible
rays.
To operate, place the unit on the sloping inlet grille. It should be positioned
centrally along the length of the grille. The power lead can then be
connected to the socket (labeled ‘UV’) on the internal wall of the cabinet.
Two safety related interlocks are incorporated into the circuit powering the
UV lamp:
(a) The front viewing panel must be fully closed, thereby achieving the
alignment of a magnet on the window with a reed switch in the rear of
the lamp casing. If alignment of the two components is not achieved,
the lamp will not function. A series of holes on the rear of the lamp
casing indicate the position of the switch; if the UV light does not work
when expected, adjust its position slightly.
(b) The fluorescent lights must be switched off. Unless these lamps are
off, it will not be possible to switch on the UV lamp.
Caution:
Any surface that is not in contact with the direct UV rays will not be
disinfected. This will include the rear and sides of the UV lamp assembly.
Therefore when the lamp is removed it must be cleaned using a proprietary
disinfectant.
4.3 FIREBOY GAS BURNER
This burner has been designed for use in Safety Cabinets and Laminar Flow
Units and can be supplied with a foot-operated switch. The switch operates
a solenoid valve allowing the gas to flow and at the same time initiates its
ignition. The burner can either be set for a fixed time of operation or can be
extinguished by pressing the foot switch a second time. The advantage of
this type of burner is that the flame is only present when actually needed and
so causes minimum disturbance to the airflow pattern within the cabinet.
4.4 TOUCH-O-MATIC GAS BURNER
This is a gas burner which is operated by a palm control. The control is

linked to the gas burner and operates a valve to allow passage of gas; a pilot
flame in the burner ensures immediate combustion of the gas. The flame is
extinguished by the release of the palm control and its size and intensity can
also be varied.
4.5 HOT WIRE ANEMOMETER
A high quality hot wire anemometer is offered for use with the Advanced Bio
Safety Cabinet.
It is necessary to check the airflow through the aperture and the downflow
velocity at the following times:
(a) On completion of installation.
(b) Whenever the cabinet is moved or disturbed.
(c) When new filters are fitted.
(d) As part of a maintenance schedule or as specified by your Safety
Officer. The frequency of this will depend on many factors but should
be a minimum of once every month.
To check airflow compliance with specification EN 12469 or BS5726 Part 1.

Refer to Section 9 ‘Calibration of Airflow and Alarms’.
4.6 BASE ASSEMBLY
For applications where bench space is not available for the cabinet, a tubular
steel base assembly is available to make the cabinet free standing. The
white base assembly is fitted with either adjustable feet or swivel castors.
4.7 DRAIN TRAY
Certain operations carried out within the Laminar Flow Biological Safety
Cabinet necessitate the use of one or more liquids and, where there is
considerable risk of spillage, a stainless steel drain tray may be utilised.
This is fitted underneath the perforated work surface so that it will not
interfere with the air flow pattern and is removable for the purpose of
cleaning and emptying.
4.8 VOLT FREE CONTACT
An option is available so that continuous remote monitoring of cabinet

operating status can be performed. Interfacing to the cabinet is via a multi-
way plug located on the top surface of the cabinet.
Parameters which affect the closing of relay contacts are:

(a) Cabinet fan on.
(b) Airflow alarm active.
Contacts are rated at maximum 1 A at 24 V a.c. or d.c. Refer to Fig.9 for

connection details.
DESIGNATION CONNECTION ALARM

A N.O Airflow Alarm On
F A
B N.C
C Common E B
D N.O Cabinet Fan On
D C
E N.C
F Common
Figure 9 Volt Free Contact Wiring
4.9 INTERNAL SOCKET RESIDUAL CURRENT DEVICE
As an additional operator safety feature, a Residual Current Device (RCD) is

available and, when fitted, is located adjacent to the circuit breaker on top of
the cabinet. The device will isolate power to the socket if a difference in
currents flowing along live and neutral conductors exceeds 30 mA.
To set (or reset) the RCD, push the black lever to the On position. It is
recommended that the RCD is checked for correct operation before each
time it is (re)set. To test, press the TEST button on the RCD once the black
lever has been set to On; the black switch should then immediately go to the
Off position.
4.10 EXHAUST KITS
A variety of exhaust ducting kits are available. Whether it is required to

recirculate the exhaust to the laboratory or duct it outside the building, an
appropriate kit contains all the necessary components. See Table 1 below
for selection.
Table 1 Exhaust Kits
EXHAUST EXHAUST EXHAUST FAN FLAP RIGID DOUBLE CARBON REMOTE FLEXIBLE EXHAUST
KIT MANIFOLD FILTER MODULE VALVE DUCTING HEPA FILTER FAN KIT DUCTING FAIL
OPTIONS MANIFOLD INC. KIT INC. 6m ALARM
MANIFOLD DUCT
Option B ? ?
Option C ? ? ? ? ? ?
Option D ? ? ? ? ? ? ?
Option E ? ? ? ? ? ?
Option F ? ? ? ?
Option G ? ? ? ? ?
Option H ? ? ?
Key: ? Item provided in kit
? Item available for use with this kit. Purchase separately.(see below for parts list).
OPTIONAL COMPONENTS
PART No. DESCRIPTION
M52262 Remote Fan Kit
M26025 Flexible Ducting 6 m
M52239 Exhaust Fail alarm
Note ?? All exhaust kits include the appropriate fitting components.
5 INSTALLATION
WARNING:
INSTALLATION SHOULD ONLY BE CARRIED OUT BY TRAINED AND
APPROVED ENGINEERS OR AGENTS.
ASTEC MICROFLOW OR ITS AGENTS CANNOT ACCEPT

RESPONSIBILITY FOR DAMAGE, LOSS OR INJURY CAUSED BY, OR
RESULTING FROM, INCORRECTLY INSTALLED EQUIPMENT.
5.1 PREPARATION FOR USE
In order to utilise the full operator and product protection factors given by the
cabinet, it is essential that the operator is fully familiar with the cabinet and
all of its functions and controls. This manual should be thoroughly studied
before the cabinet is used.
A requirement for the ‘GS’ approval of EN 12469 models is that access to

critical functions such as the fan on/off controls should be protected to
prevent unauthorised use. Pressing any of the fan controls (switches
annotated 1, 2 and 3 in Fig. 2) will prompt the user to enter the four digit PIN
code. Only after acceptance of this code will the change in operating state
of the unit be actioned. Entry of an incorrect code, or failure to start to enter
a code within 14 seconds results in no change to the operating state of the
cabinet.
When commencing use, the following procedure must be followed for safe
operation; abbreviated instructions are shown on the Control Panel:
(a) Plug in the power lead, if applicable. Switch on power at the switch
located at the power entry point.
(b) Confirm that the blue LED on the control panel is lit, indicating that
mains power is applied to the cabinet.
(c) Press and hold down the ‘TEST’ button. All LEDs on the keypad
should light and the buzzer should sound. If not, the cabinet should not
be used and maintenance advice sought.
(d) With the Front Sliding Window and frame in the closed position, press
the ‘FAN’ button. If prompted, enter the PIN number. Entering the
correct number then allows the fan to be switched. The LED next to
the fan button pressed will light and the display will show the message
‘WARNING: SCREEN LOCKED SHUT’.
(e) Release the door frame by releasing both handles on the door frame
allowing the window to move just clear of the window seal. Lift the
window to the fully open position. Tighten the door frame back to its
closed position by tightening the two frame handles.
An intermittent beep will be heard and the display will show the
message ‘STABILISING FLOW’. This stabilising phase allows the
fan(s) to build up speed and for the airflows to settle at their operating
level. It is important to remember that a safe operating condition is not
achieved until the display states ‘CLASS II CABINET SAFE’. To avoid
unnecessary nuisance to the operator, airflow alarms are suppressed
during this stabilising phase.
(f) It is possible that after the stabilising process, the buzzer sounds and
the display advises of an airflow alarm. The possible causes are as
follows:
(i) The first time the cabinet is switched on following a maintenance
operation involving use of the set up software.
(ii) The environment of the cabinet is at the extremes of its operating
temperature (10 - 30°C).
(iii) If the cabinets airflow pattern has been recently disturbed, e.g.
window closed down or exhaust obstructed.
In any of these cases, the alarm can be expected to clear and display
report a safe condition after waiting a few extra seconds.
(g) If an airflow alarm is still displayed after 3 minutes from switching on
the cabinet, the unit should not be used. The window must be moved
to the closed position and advice sought from you Safety Officer or
Supplier.
(h) Other safety alarms that are self-explanatory if the display is consulted
include:
(i) The sliding window frame is unsecured.
(ii) The window is locked in a position other than the correct setting.
(iii) The filters need replacement.
(iv) Failure of sensors.
Alarm condition (iv) is the only situation that should prevent use of the
cabinet since safe flow conditions cannot be confirmed.
6 DECONTAMINATION OF THE CABINET
6.1 SWABBING DOWN
It is recommended that the cabinet be kept clean and free of dust by

swabbing down or washing down with a suitable disinfectant. Disposable
gloves should be worn for this task.
6.2 FUMIGATION (see also Appendix 4)
Fumigation would be necessary at the following times:

(a) Before carrying out any maintenance work.
(b) Before changing high efficiency filters or prefilter (if fitted).
(c) Before performance testing is carried out.
(d) Before moving, relocating or in any way disturbing the cabinet.
(e) Before instituting a change in the work program.
(f) After a gross spillage - as defined by your Safety Officer.
(g) To decontaminate any equipment or materials before their removal
from the cabinet.
(h) Periodically as required by your Safety Officer.
A Formalin Vaporiser (see Fig. 8) is fitted (as an option) to the side wall of
the unit. The fumigation of the cabinet is carried out as detailed in the
following procedure:
Notes:
(1) Formaldehyde is hazardous: observe handling procedures.
(2) The following procedure is provided for guidance purposes only; your
Safety Officer should be consulted for definitive guidance.
(3) The use of formaldehyde for sanitising cabinets is prohibited in

Germany under law except in special circumstances. Regulations
TRGS 511 and 522 detail this point.
6.2.1 Manual Fumigation Using the Formalin Vaporiser
For quantities of the solutions used in the following procedure, refer to

Appendix 5.
Procedure
Open the cabinet.
Attach the trunking of the Formaldehyde Extraction Unit to the air outlet on
the top of the cabinet.
Add the formaldehyde solution to the deionised water. This must be carried
out in a fume cupboard or Class I safety cabinet (vented outside).
Remove the cap of the vaporiser, add the diluted formaldehyde solution and
replace the cap.
Press the VAP switch and enter 2222.
Close the cabinet window. The vaporiser operates automatically and takes
approximately 30 minutes to evaporate the contents.
The Sterilising cycle is set at 240 minutes. After this time the cycle changes
to ‘Purging’ (set for 480 minutes).
Onced ‘Purging’ is indicated on the display, switch on the Formaldehyde

Extraction Unit and after 10 to 15 seconds open the orange admission valve
on top of the cabinet and allow the unit to run for the purging time.
Periodically check the room with a formaldehyde meter (this should read
<0.2 ppm formaldehyde).
After purging, switch off the extraction unit and close the orange valve.
Remove the trunking from the cabinet.
Run the cabinet as normal and check the room with the formaldehyde meter.
6.2.2 Automatic Fumigation
Procedure
(If the cabinet is fitted with Automatic Fumigation Cycle see Appendix 4)
Release the two door frame handles. The frame will release and move 20 -
30 mm outwards.
With both sides released slide the glass fully down using the handles.
When the window is in position, tighten the two door frame handles to secure
the window.
If the cabinet is installed in the re circulatory mode, fit a temporary exhaust

outlet and trail to a nearby window or wall aperture leading to the outside of
the building. If the cabinet is installed as the recirculating type without a
secondary exhaust filter fitted, then a suitable transition piece to enable the
duct to be fitted will need to be sourced.
Ensure that the cabinet is free of Hydrochloric acid and other Chloro
compounds. This will avoid the production of the carcinogenic product that
results from the mixing of formaldehyde and HCl or Chloride gas.
Employing suitable handling procedures, dispense the appropriate amount of
Formaldehyde solution 40% w/v (see Appendix 5) together with an equal
volume of water into the vaporiser.
Press the ‘VAP’ switch on the control panel. After entry of the code number
(‘PIN’), the fumigation cycle will commence, unless the window is detected
as being unlocked. Throughout the cycle, the display will report progress
and will prompt for any action that is required. A description of the cycle is
provided in Table 2 below:
Table 2 Fumigation Cycle

ACTION PROCESS DISPLAY MESSAGE
Press VAP Start Cycle ‘Enter PIN Number’

Enter four digit Number. Vaporiser powered to Evaporation: xx minutes to run.
evaporate formalin.
Vaporiser powered for xx*
minutes.
Fan circulates fumigant for 5 ‘Circulating’
seconds.
Formalin sterilises inside of ‘Fumigating’
the cabinet.
‘Purging required: open window’
Open window leaving a Fan will start to purge ‘Purging’
gap of about 25 mm. fumigant from cabinet.
When purge time is expired, ‘Stabilising Flow’ (on expiry of
the cabinet will resume purge timer), followed by ‘Class
normal operation, thus door II cabinet safe’ (or similar when
position alarm will sound window correctly positioned).
* ‘xx’ Denotes preconfigured time which counts down in minutes.
As it can be appreciated, the fumigation cycle is straightforward by virtue of

the display reporting status and prompting for action when necessary. A
safety feature built into the control system is that if the window is disturbed
during the evaporation or fumigating processes, the fan will automatically be
switched to 100%, in order to safely expel the fumigant to outside the
laboratory. It is consequently a good idea to fit the exhaust duct at the stage
recommended. In such a situation the fan will continue to run at full speed
until the key button is pressed and the fumigation PIN number has been
entered for a second time.
Finally, the exhaust duct can be removed from the cabinet.
6.3 GASSING THE LABORATORY
Should this be required at any time, full information is given in the DHSS
Memorandum ‘Control of Outbreaks of Small-pox’ 1975, and advice should
also be taken from your Safety Officer.
It should be noted that Formaldehyde vapour in normal dilutions will not
affect any part of the cabinet or its controls.
Note:
It is the responsibility of the user to ensure that whatever means of cabinet
decontamination is used, it is compatible with the material being handled and
has been approved by the Safety Officer.
7 MAINTENANCE
The Laminar Flow Biological Safety Cabinets have been designed to give
many years’ trouble-free efficient service and to keep maintenance to a
minimum. However, to ensure this, they must be regularly cleaned and
checked.
A full maintenance service is offered by Astec Microflow’s approved service

company. The British Standard for Biological Safety Cabinets (BS 5726)
recommends that users utilise this service from the manufacturer or their
authorised agents. Overseas, this same service is offered by the Astec
Microflow distributors.
In addition to this, however, operators should carry out the following

procedures on a schedule agreed with the Safety Officer, so as to ensure
that the cabinet is always in first-class working order.
7.1 DAILY/FREQUENTLY
(a) Keep the unit clean externally as well as internally. It should be wiped
over with a damp cloth using a proprietary bactericidal solution. The
front viewing panel should be kept clean so that the operator always
has a clear view of work being performed inside the cabinet.
(b) Ensure that the audible alarm and all indicators are functioning by
pressing the ‘TEST’ button. Any faults should be corrected before the
cabinet is used.
(c) Check the airflow in the unit and at the exhaust filter as described in the
relevant standard. The values should be as detailed in Appendix 5.
(d) Visually check that the exhaust filter is free of any obstruction that may
impede airflow.
7.2 WEEKLY/MONTHLY
In addition to the above the following checks should be carried out:

(e) Visually check for damage to the seal on the front of the cabinet which
is compressed by the glass of the front window.
(f) Inspect all mains cable to the cabinet, or to any extras that may have
been fitted, for damage or wear. In the event of any fault being
discovered, contact an Astec Microflow approved service company.
7.3 MAIN AND EXHAUST FIL TER SERVICING
The protocol for testing, monitoring, replacement and disposal of filters is

detailed in Appendix 7.

7.4 FLUORESCENT TUBE REPLACEMENT
7.5 UV TUBE REPLACEMENT
7.6 CIRCUIT PROTECTION

8 CONTROL SYSTEM: CUSTOMISATION AND CALIBRATION
The microprocessor based control system used in the cabinet allows the
user two main advantages over the conventional analogue system. These
are:
(a) Cabinet functions can be customised to the user’s requirements.
(b) Calibration can be performed remote from the cabinet itself, without the
need to access electrical components located in a potentially
hazardous compartment.
Full details are contained in the Maintenance Manual held by the approved
service provider.
This should only be undertaken by an approved and trained engineer.

9 CALIBRATION OF AIRFLOW AND ALARMS
service provider.

10 ADDITIONAL AIRFLOW SET UP FUNCTIONS
service provider.

11 SPARES LIST
Refer to the Supplier or the Maintenance Provider for the supply and
installation of spares.
MODEL ABS 1000 ABS 1200 ABS 1500 ABS 1800

Main Filter MSABS1000-031 MSABS1200-031 MSABS1500-031 MSABS1800-031
Exhaust Filter MSABS1000-030 MSABS1200-030 MSABS1500-030 MSABS1800-030
Light Tubes 257-0504 257-0383 257-0503 257-0502
UV Tubes 257-0512 257-0511 257-0511 257-0511
Gas Springs 754-1026 754-1023 754-1022 754-1021
ITEM QTY. USED DESCRIPTION PART No.

Fan 2 Motor Fan Unit complete with Capacitors BKA10548E
Starters for UV and 3 Starter BHE25013E
Fluorescent Tubes
Fuses 2 10 A Antisurge EHB 10176 E
Fuses 3 6.3 A Quick Blow EHB 10342 E
Fuses 2 1 A Quick Blow EHB 10437 E
Fuses 1 100 mA Antisurge EHB 10174 E
Fuses 1* 100 mA Antisurge EHB 10174 E
Fuses 1* 250 mA Antisurge EHB 10432 E
Door Seal 6m Seal AFA 10030
Circuit Breakers 1 Double Pole Circuit Breaker/Switch (2.5 A) BHD 51522 E
ABS PCB 1 Main circuit board 259-0994
ABS Top Box Duct Spigot 1 Duct Spigot 165-2000
Flex Ducting - Flex Ducting 150-0300
* Used on volt free contact option
SPARES LIST - AUTOMATIC FUMIGATION SYSTEM
ITEM QTY. USED DESCRIPTION PART No.

Filter 1 Carbon ABS 013
Fan 1 230 V 50 Hz 3155-100018
Valve Flap 2 Rubber M53499
Valve Solenoid 2 230 V 50 Hz 3225-100001
12 SERVICING RECORD
DATE ACTION COMPLETED BY

APPENDIX 1 - NOTES ON COSHH REGULATIONS (UK ONLY)
1 The "Control of Substances Hazardous to Health" (COSHH) regulations,

effective from 1st October 1989.
2 The regulations are the UK implementation of an EEC Council Directive

80/1107/EEC.
3 The regulations require an employer to protect his employees and any other
people (whether working for him or not) from hazardous substances.
4 A hazardous substance is defined as:

(a) A substance which is on the list of hazardous substances as defined by
the Classification, Packaging and Labelling Regulations 1984 (b).
(b) A substance for which an Occupational Exposure Limit (OEL) value
exists. This list is similar to US Threshold Limit Value levels (TLV).
(c) A micro-organism which creates a health hazard.
(d) Dust at a substantial concentration in air.
(e) Any substance which creates a hazard to health, similar to the hazards
created by the substances in (a) to (d).
Note:
Paragraph 4 (e) is a "catch-all" section.
5 The employer is responsible for assessing the risk to an employee.
6 The employer must prevent or control the exposure of an employee to

hazardous substances.
7 The control of exposure "shall be secured by measures other than the

provision of personal protective equipment". This means the fumes must be
contained, rather than providing protective suits and masks to staff.
8 OEL values must not be exceeded.
9 The employer must ensure that safety equipment is properly used.
10 The employee must use safety equipment provided correctly.
11 The employer must maintain safety equipment in good working order; in

particular:
(a) Exhaust ventilation equipment must be examined every 14 months.
(b) Other safety equipment must be examined at "suitable intervals".
(c) Records of checks, tests and repairs must be kept for 5 years.
12 Monitoring of exposure to hazardous substances must occur "in accordance

with a suitable procedure". Records of results must be kept for 5 years for
general monitoring and for 30 years when they relate to a specific employee.
13 Regular medical checks are required when working with certain listed
substances, or where an identifiable disease is associated with a certain
substance.
14 An employer must provide suitable instruction and training to employees
regarding risks of substances and precautions to be taken.
15 Certain other regulations take precedence, such as Control of Lead at Work,
Control of Asbestos at Work, radioactive, explosive or flammable
regulations, Mines and Quarries Act, and medical treatment regulations.
APPENDIX 2 - UK APPROVED SERVICE COMPANIES TELEPHONE AND FAX NUMBERS
We only recommend BIOQUELL Service, as this company has been fully assessed for competency, trained engineers and
insurance cover for our complete product range.
COMPANY NAME TELEPHONE FAX PRODUCTS EMAIL

BIOQUELL Service Rod Sarvari 01264 835 800 01264 835 801 A,B,C,D,F support@bioquellservice.com
Product codes: A All Airflow

B Biological Safety Cabinets
C Laminar Flow Cabinets
D Safescope Medical Range
E Limited Airflow Products
F Genesis Models Updated on 05/04/01
BIOQUELL Service is our sister company which we use for installation, commissioning and warranty work. BIOQUELL Service is
the only approved trained company for Genesis models. There are however other companies who have trained engineers.
APPENDIX 3 - CARE AND CLEANING
1 BIO ORGANISM REMOVAL CLEANING
Special attention for cleaning must be given to the door seal the
recommended method is to fully remove the rubber door seal and clean
thoroughly with a chlorine based disinfectant. Then clean the exposed
metal/plastic liner as described below and finally thoroughly wash the rubber
door seal with sterile water and refit.
All MSCs should be kept clean and free of unnecessary equipment. The
interior should be swabbed after use with a suitable disinfectant. Phenolics,
quarternary ammonium compounds and aldehydes can be use for
disinfecting these surfaces. Users of chlorine compounds should be aware
of their corrosive nature. The use of alcohols should be undertaken with
caution because of the risk of fire. Heavy duty polyvinyl chloride (PVC) or
rubber gloves which provide suitable protection for the hands and wrists
which can be disinfected for re-use should be worn when cleaning MSCs.
Note 1:
In cases where fumigation may be ineffective, it may be necessary to have
MSCs designed to allow contained removal for suitable dispersal, for
example incineration.
Note 2:
At the selection of disinfectants occupational health aspects should be
considered.
2 STAINLESS STEEL COMPONENTS
Considerable care has been taken in the selection and processing of the
stainless steel components used in the construction of this equipment,
however even stainless steel can be damaged by chemical attack.
It is therefore important to ensure that any cleaning or disinfecting

procedures used will not cause a chemical attack that may damage the
surface of the stainless steel. Work surfaces should be kept clean and free
of chemical liquids, particularly those containing Chlorine.
Liquids or vapours containing Chlorine are known to cause gradual staining

of stainless steel. To avoid this, when chemicals are used containing
Chlorine all surfaces should be thoroughly dried and all traces of the solution
removed, the area should then be treated with a neutralising agent. Open
vessels left inside the equipment which are likely to cause Chlorine vapours
may also result in staining of the metal surfaces.
Should the surface of the stainless steel become stained, it may be cleaned
by mechanical polishing and special treatment of the affected area. Our
service department can help with this process.
Note:
When cleaning to remove bio organisms the method we recommend is to
wash with 40% ethanol or equivalent in all poor access points, e.g. corner of
liners, fold and recesses in the tray. It is important to ensure that full surface
contact is achieved and the use of cotton buds is recommended.
3 PLASTIC COMPONENTS
Cleaning of the plastic items should only be carried out with mild detergent
or chlorine based cleaning solutions. Care must be taken not to apply
chlorine solutions to any stainless steel metalwork, as this will potentially
cause staining.
Do not apply heat, abrasive materials, solvents or solvent wipes to the

surface as this can cause irreparable damage.
4 PAINTED AND OTHER COMPONENTS
Caution:
Do not use water around electrical connections, switches etc.
To clean all painted and other components use a damp cloth and, when
required, a liquid detergent.
Note:
Do not use abrasive cleaners.
APPENDIX 4 - AUTOMATIC FUMIGATION
1 INTRODUCTION (see also para 6.2)
The Astec Microflow automatic formaldehyde fumigation system has been

designed for incorporation in the biological safety cabinet. It is controlled by
the cabinet control system and the fumigation cycle is completed
automatically and operation functions are displayed on the control LED
The fumigation section of the equipment manual details the methods of

fumigation.
There are a number of additional functions associated with the automatic

system, these are detailed in this Appendix.
2 FUMIGATION METHOD
WARNING:
AFTER FORMALIN DECONTAMINATION ASTEC MICROFLOW
RECOMMEND THAT THE FAN IS SWITCHED ON BEFORE EACH TIME
THE CABINET IS OPENED. THERE ARE RECORDED CASES OF
PARA-FORMALDEHYDE FORMATION WITHIN THE CABINET DURING
DECONTAMINATION. THIS PARA-FORMALDEHYDE CAN TAKE UP TO
SEVEN DAYS TO DISPERSE WITH THE CABINET SWITCHED ON.
2.1 Close the front window and tighten both handles. A notice should be
attached to the cabinet to indicate that the fumigating cycle is in operating.
THE CYCLE MUST NOT BE STOPPED. The fumigation cycle will not
commence unless the window is detected as being locked.
2.2 Ensure that the cabinet is free of hydrochloric acid and other chemical liquids
that may contain chlorines.
2.3 Employing safe handling procedures, dispense the correct amount of

formaldehyde solution 40%w/v (see Appendix 5) together with equal
amounts of water into the vaporiser. The amount of formaldehyde will
depend on the size of cabinet being treated, check the table on the side of
the vaporiser.
2.4 Press the VAP switch on the control panel after entering PIN code (VAP
standard code is 2222 ). Throughout the cycle the control display will
report progress, the description of the phases of the cycle can be found in
the manual.
2.5 During the cycle the control display will show the following information:
(a) Evaporation time to run, in minutes.
(b) Fumigation time to run, in minutes.
(c) Purging time to run, in minutes.
(d) Cycle complete.
2.6 The fumigation cycle should be stopped by using the VAP switch, the
window can then be unlocked and the cabinet set to normal operation.
Caution:
The operation of the Astec Microflow automatic fumigating system does not
require ventilation to an exhaust system, however, IT IS NECESSARY TO
PROVIDE VENTILATION FOR THE ROOM. It is recommended at least two
air changes per hour, this will ensure that all traces of formaldehyde are
removed from the room.
3 CARBON FILTER CHANGING
The carbon filter will complete a minimum of ten fumigating cycles.
On fitting a new carbon filter, the cycle councillor will require resetting in the
following manner:
(a) Press the key switch and enter the VAP code 2222.
(b) Select 2, reset cycle counter to zero.
The removed carbon filter will be contaminated with formaldehyde. Handling

of the filter will require suitable personnel protection equipment, consult your
Health and Safety Officer.
Environmental regulation for the disposal of such material may be

applicable, consult your Health and Safety Officer. The new carbon will be
supplied with a sealable bag in which the used carbon filter should be put as
it is removed from the cabinet.
4 STANDARD TIME SETTINGS
The Class II cabinet will be set for the following timings:

(a) Vaporiser 30 minutes.
(b) Fumigating 240 minutes.
(c) Purging 480 minutes.
Testing within Astec Microflow has shown that these control times ensure
the cabinet is purged to less than 1 ppm at the end of the fumigating
process. Total time 12 hours 30 minutes.
APPENDIX 5 - MODEL AND SPECIFICATION DATA
MODEL ABS 1000 ABS 1200 ABS 1500 ABS 1800
HEIGHT (mm) 1370 1370 1370 1370
DEPTH (mm) 735 735 735 735
WIDTH (mm) External 1187 1387 1687 1987
Internal 1000 1200 1500 1800
WEIGHT (kg) 170 180 210 230
RECOMMENDED
FUMIGATION QUANTITIES
FORMALIN (ml) 17 22 26 30
WATER (ml) 17 22 26 30
POWER CONSUMPTION (kW) 0.5 0.65 0.85 1.3

APPENDIX 6 - SAFETY SYMBOLS
BIOHAZARD
CAUTION
CAUTION, RISK OF ELECTRIC SHOCK
FAN
EARTH (GROUND) TERMINAL
PROTECTIVE CONDUCTOR TERMINAL
POWER
ON/OFF
I O
APPENDIX 7 - PROTOCOL FOR TESTING, MONITORING, REPLACEMENT AND
DISPOSAL OF FILTERS
THIS INSTRUCTION SUPERSEDES ALL MANUAL INSTRUCTIONS REGARDING

FILTER CHANGE PERIODS AND METHODS OF TESTING.
1 BEST PRACTICE
In order to clarify best practice for the use of carbon filters Astec Microflow
would like to recommend the following guidance. This guidance is primarily
for Carbon filters but High Efficiency Particulate (HEPA) filters are referred to
when appropriate.
2 FILTRATION
Carbon filter technology has been safely applied for the absorption of
harmful vapours and fumes for many years. However, there still exists some
doubt with users as to the best practice with regard to filter monitoring and
changing. Astec Microflow has detailed best practice in the following product
group Tables 1, 2 and 3. Each of these tables express recommended test
monitoring and change periods in months, (assuming a 40 hour working
week).
3 TEST PROTOCOL - TERMS
The test period is the maximum period between the filter installation test and
the next test for fume bypass of the filter, in some applications where heavier
loads of chemicals are applied or the chemicals are dangerous then filter
testing should be completed weekly or monthly.
The monitoring period is the maximum period between the filter installation
test and the regular weekly/monthly filter monitoring for fume bypass.
The change period is the maximum period between the filter installation and
the time to change the filter.
All the test, monitor and change periods are expressed in months.
Therefore, in units of higher usage the month recommendation will be
superseded by the earlier number of hours.
Note:
The test, monitor or change should be completed at the earliest point
whether this be, for example, 6 months or 1000 hours which ever is sooner.
4 CARBON FILTER LIFE
It is important to note that once the sealed bags containing carbon filters
have been opened they will absorb water from the atmosphere. Therefore
after 24 months the filters potential life cannot be assured and should be
replaced.
The filter test protocol should be applied during all test, monitoring, and filter
change stages. The protocol is to confirm that the filter is correctly fitted and
is absorbing the fumes intended.
It is best practice to regard our guidance time scales as Maximums.
Tables 1, 2 and 3 detail each product and the relevant test, monitor, and
change periods for the main and safety filters.
Months to Hours conversion

1 month = 175 hours
3 months = 500 hours
Note:
Use the shortest time scale that applies.
5 FILTER TEST PROCEDURE
After installation or when test or monitoring is required the following

procedure should be completed:
(a) Ensure the unit is switched on and confirm the airflow is correct by
measurement or examination of the low airflow alarm.
(b) Introduce the chemical challenge that is normally in use within the unit
or a less harmful equivalent for example Iso Propyl Alcohol (IPA).
(c) Test the exhaust or the filter test port to confirm there is no evidence of
the chemical.
If the chemical has been detected then the following checks should be
completed.
This test can be simply carried out using Gastec or Draeger detector
tubes and the appropriate hand held pump. Test pumps and tubes can
be supplied by Astec Microflow Ltd.
(d) Note for some chemicals the chemical tube technique is not the best
method for measurement. For example, Glutaraldehyde should be
tested using OSHA 64 (American standard) method for accuracy.
Notes:
1 Ensure the filter is correctly fitted with the seal seated and compressed.
2 Ensure that there are no gaps in the filter seal.
3 Change the filter and retest.
4 If the chemical by pass continues contact your agent, service provider
or Astec Microflow, Tel +44 (0) 1934 410500.
6 FILTER DISPOSAL
The only fully safe method of disposal of carbon and HEPA filters is
incineration at the temperatures used for medical waste. However it is
possible to dispose in some cases safely to land fill with other laboratory and
medical wastes. Astec-Microflow recommends incineration as best practice.
Incineration is available from several companies including P & R Disposal

Services Ltd. and Shanks Ltd.
Table 1 - Airflow Fume Cupboards

MAIN FILTER SAFETY FILTER CARBON ‡
MODEL
TEST MONITOR CHANGE TEST MONITOR CHANGE
A3000 6 9 12 Not applicable
Astec EVE 6 9 12 Not applicable
Condor Monitor monthly 12 Not applicable
Cytolab ** 8 uses or 24 months Not applicable
Downflow Monitor monthly 12 6 12 24
Ducted Not applicable Not applicable
EDU 96 † 6 9 12 6 12 24
Exair † 6 9 12 6 12 24
Forensic 6 9 12 6 12 24
Mobile evacuation ** 8 uses or 24 months Not applicable
Monair 05 6 9 12 Not applicable
Monair 08 6 9 12 Not applicable
Monair 10 6 9 12 6 12 24
Monair 15 6 9 12 6 12 24
Monair 20 6 9 12 6 12 24
Monair 25 6 9 12 6 12 24
Monair 30 6 9 12 6 12 24
Monair special 6 9 12 6 12 24
Omni Cytotoxic Not applicable HEPA Not applicable HEPA
Omni Lam flow Not applicable HEPA Not applicable
Omni PCR Not applicable HEPA Not applicable HEPA
Omni TC Not applicable HEPA Not applicable HEPA
Sensair 10 6 9 12 6 12 24
Sensair 15 6 9 12 6 12 24
Sensair 20 6 9 12 6 12 24
Sensair 25 6 9 12 6 12 24
Sensair 30 6 9 12 6 12 24
‡ Applies only when carbon safety filter is fitted.

† In addition apply CLEAPPS test protocols(UK standard).
** Not used continuously therefore calculated life based on absorption capability.
Table 2 - Endoscopy Range
Astec Microflow recommend that Endoscopy HEPA exhaust filters are
changed every 24 months.
MAIN FILTER SAFETY FILTER (CARBON) ‡
MODEL
TEST MONITOR CHANGE TEST MONITOR CHANGE
MD 6 9 12 Not applicable HEPA
MDV 6 9 12 Not applicable HEPA
Mini F 1 1 3 1 1 6
Mini MD (f) 1 1 3 1 1 6
MP 6 9 12 Not applicable HEPA
Multi F 1 1 3 1 1 6
Safescope Mini PR 6 9 12 Not applicable HEPA
Safescope PR 6 9 12 Not applicable HEPA
Safescope Mini R 6 9 12 Not applicable HEPA
Safescope R 6 9 12 Not applicable HEPA
Water system Not applicable Not applicable
‡ Applies only when carbon safety filter is fitted.
Table 3 - Biological and Laminar Cabinets

HEPA exhaust and safety filters to be changed when airflow is reduced or
breakthrough is detected.
MAIN FILTER CARBON OPTION K**
MODEL
TEST MONITOR CHANGE CHANGE
Laminar Flow
M10158
M25674
Not Not
M25676
Applicable Applicable
M40004 HEPA HEPA
M50480
M50545
M50546
M50548
M50549
M50713 6 9 12 10 uses or 24 months
M50715 6 9 12 10 uses or 24 months
M51423 6 9 12 10 uses or 24 months
M51424 6 9 12 10 uses or 24 months
M51426 6 9 12 10 uses or 24 months
M70085 6 9 12 Not Applicable
Microaire Not applicable HEPA 10 uses or 24 months
Safety Cabinet 6 9 12 10 uses or 24 months
ABS 1000 6 9 12 10 uses or 24 months
Special SK 6 9 12 10 uses or 24 months
Tissue Culture Not applicable HEPA 10 uses or 24 months
** Not used continuously therefore calculated life based on absorption capability.

APPENDIX 8 - INSTALLATION GUIDANCE
Upon installation the approved service provider will provide the following
documentation which MUST be retained by the user:
(a) A test certificate detailing compliance with all parts of EN 12469.
(b) A certificate of HEPA filter/seal leaktightness of the cabinet at the place of
use.
EXTRACT FROM BS 5726 : PART 2 : 1992
4 RECOMMENDATIONS FOR INSTALLATION
4.1 CABINETS
4.1.1 The installation of cabinets and their commissioning will normally be the
business of the supplier or an experienced agent. Local engineers may
need to be consulted to determine suitable routes to duct filtered air to the
outside.
4.1.2 For containment level 3 laboratories where there is an installation problem

because of difficulties in exhausting cabinet air to the outside, specialist
advice should be obtained.
4.1.3 Operator protection offered by a cabinet can be adversely affected by poor

siting of the unit. It is important to avoid siting a microbiological safety
cabinet in a thoroughfare or in line with a doorway or openable window.
Preliminary airflow visualization tests may help to select the optimum
position within a room. Performance is verified by conducting operator
protection tests once the cabinet is in position and running (see BS 5726 :
Part 3). In difficult conditions, it may be helpful to make a temporary
installation of the cabinet in order to make these tests before the final
installation.
4.1.4 The siting of safety cabinets in laboratories should be considered at the

initial stages of the planning of a new building or before modification of an
existing building. If additional safety cabinets are required in an existing
laboratory, the recommendations given in this clause also apply. The site
selected should comply with the interrelated requirements for containment,
extraction, room make-up air ventilation and general environmental safety
and comfort. The recommendations for siting given here are only intended
to act as a guide to the avoidance of disturbances to the safety cabinet, its
operator and other personnel and to the avoidance of interference with
escape routes, etc. No specific dimensions or planning arrangements can
prospectively guarantee satisfactory performance of the cabinet in all cases.
4.1.5 If cabinets are joined together it is strongly recommended that the junctions
should be airtight and should not put undue stress on the cabinets or any
junction or adaptor pieces. Sealing gaskets or mating surfaces should not
leave internal cavities or crevices.
Note:
Attention should be paid to vibration as if it is excessive damage to seals and
gaskets may occur.
4.1.6 To avoid disturbances to the safety cabinet and its operator, consideration
should be given to the following.
(a) The distance from the plane of the aperture to any circulation space
should be at least 1000 mm, so as to preserve a zone undisturbed by
anyone other than the operator (see figure 1(a)).
(b) The distance between the front aperture and a bench opposite should
ideally be at least 1500 mm (see figure 1(h)). However, containment
performance is not likely to be affected if this distance is reduced, for
instance to enable an operator to use the bench behind, whilst working
at the cabinet. In this case operator movement over a smaller distance
may cause less air disturbance. With such procedures their effect on
containment should be measured by relevant operator protection factor
tests.
(c) There should be no opposing wall (or other obstruction likely to affect
the airflow) within 2000 mm of the front aperture (see figure 1(c)).
(d) Safety cabinets should not be installed in positions where they are
likely to be affected by other items of equipment. In particular the
distance to the aperture of an opposing safety cabinet, fume cupboard,
or the edge of a local exhaust ventilation outlet should be not less than
3000 mm (see figure 1(d)).
(e) Any room air supply diffuser should not be within 1500 mm of the front
aperture. If there are large numbers of safety cabinets in a laboratory
this recommendation may be difficult to comply with, but where
diffusers have to be placed in close proximity to a safety cabinet, their
discharge velocities and therefore air handling rates will need to be low.
(f) A safety cabinet should not be positioned with either side closer than
300 mm from a wall or similar obstruction (see figure 1(e)).
(g) Large obstructions (e.g. an architectural column) projecting beyond the
plane of the aperture should not be within 300 mm of the side of the
safety cabinet (see figures 1(f) and (g)).
(h) Doorways should not be within 1500 mm of the aperture or within
1000 mm of the side of the safety cabinet (see figure 1(h)) except
where a door includes air transfer grills in which case protection factor
testing should be carried out to ascertain a suitable distance.
4.1.7 The position of a safety cabinet should satisfy the spatial requirements (e.g.
vision, lighting and convenience of access) of the operator and personnel
working nearby. When a cabinet is installed on a bench top, the leading
edge should be flush with or slightly overhanging the edge of the bench top.
Note 1:
Typical problems and their solutions are illustrated in figure 2.
Note 2:
There should not be an open space between the leading edge of the cabinet
and the front of the bench as this may create turbulence in front of the
aperture. It also provides an obstacle which could adversely affect airflow
across the cabinet face.
4.2 DUCT INSTALLATION
4.2.1 Cabinets should exhaust air from integral fans by a direct airtight duct less
than 2 m in length. If such short duct connections are not possible the
exhaust fan should be situated separate from the cabinet and close to the
discharge end of the duct so that any substantial length of ducting is under
negative air pressure.
4.2.2 The air extraction system should be capable of dealing with external wind
pressure up to 250 Pa with extra capacity of not less than 200 Pa for
blockage compensation.
4.3 CABINET EXTRACTION SYSTEM
4.3.1 General
The extraction system (together with the arrangements for make-up air)
should be so constructed as to minimize the effect on the safety cabinet of
outdoor wind (up to pressures of 250 Pa at the end of the duct) and other
sources of air disturbance. The discharge opening should be sited with due
regard to the flow pattern of air around the building and should be located to
minimize the risk of exhaust air being drawn back into the building or into
neighbouring buildings through open windows or air intakes.
4.3.2 Cabinet Ductwork
4.3.2.1 Ductwork should have a smooth, obstruction free interior and should
preferably be circular in cross section without excessive changes in
diameter. The extraction ductwork should be clearly marked with labels
indicating a biological hazard complying safety sign A. 2.10 of BS 5378 :
Part 3 : 1982.
4.3.2.2 The cross section of the ductwork and the routing of the ducts within the
building should be such that the noise level is within the requirements of the
laboratory (see clause 10 of BS 5726 : Part 1 : 1992). However in some
cases, e.g. long duct runs or large volume cabinets, considerations of space
may require smaller duct diameters and consequently give air velocities in
excess of this guideline. If the purchaser requires a noise level lower than
that specified in clause 10 of BS 5726 : Part 1 : 1992 it is recommended that
air velocities should not exceed 5 m/s for each internal duct or 6 m/s for
external ducts.
4.3.2.3 Ductwork should follow the most direct route from the cabinet to the
discharge point, and it should be possible to identify the ductwork leading
from a particular cabinet. Bends should be kept to a minimum number and
have the largest radii practicable. There should be a minimum of horizontal
run.
The ductwork should be designed to accommodate thermal expansion and

contraction. Ductwork should not violate the fire compartmentation of the
building in passing between the safety cabinet and its final discharge point.
If possible, fire dampers should be avoided and fire protection provided by
means of suitable treatment of the duct work, or by enclosure of the duct
within a protected shaft in accordance with the recommendations in BS
5588 : Part 9 or BS 8313, or by running the ductwork outside the building. If
dampers have to be fitted they should be upstream of the fan.
4.3.2.4 Whilst no material is completely resistant to all forms of chemical attack,

materials of construction for the ductwork should be chosen to give the best
resistance to the chemical and physical conditions to which they will be
subjected, with due consideration to disinfection agents that may be used,
and that the cost and practicability will allow.
Note:
Attention of installers is drawn to the provisions of the Building Regulations
1985 : Part B Approval document (and any subsequent legislation) regarding
the effect of the installation on compartmentation of the building and on the
provision of fire stops, and to the corresponding parts of the Building
Standards (Scotland) Consolidated Regulations 1971 (Statutory instrument
No. 2052 and the corresponding part of the Building Regulations (Northern
Ireland) 1977 (Statutory instrument No. 149).
4.3.3 Negative Pressure Laboratories
In situations where microbiological safety cabinets are installed in negative

pressure environments a pressure differential greater than 40 Pa can
develop between the cabinet working space and the laboratory when the
cabinet is turned off. This could result in air flowing out of the safety cabinet
aperture and potentially a risk of formaldehyde leaking out during fumigation.
This difficulty can be overcome by installing a total shut-off damper in the
exhaust ducting. A leakage test should be conducted with this damper
closed and the laboratory operating at the designed degree of negative
pressure.
4.4 MAKE-UP AIR SYSTEMS
4.4.1 Safety cabinets should be installed only after first considering the details of
the make-up air system necessary to replace all of the air entering the safety
cabinets in the laboratory. The safety cabinets and other air extraction
points together with the make-up air supply in the laboratory should be
regarded as an integral system. The air supply system should not
compromise the performance of the safety cabinets.
4.4.2 The high air change rate in the laboratory resulting from a multiple safety
cabinet installation, or from a single installation in a small laboratory,
necessitates careful selection of the method of supplying make-up air.
Insufficient space may preclude the use of conventional equipment, and
special diffusers, grilles, or a perforated ceiling may be required to achieve
low room-air velocities.
4.4.3 Arrangements for the supply of make-up air should be consistent with the
requirements for protection from fire and smoke and with the achievement of
the environmental conditions specified for the laboratory. Make-up air
should be heated or cooled, as necessary, to maintain a laboratory air
temperature within ± 2 °C of that required. The introduction of make-up air
should avoid draughts in the area of safety cabinets.
4.4.4 For air make-up by passive air inflow, openings, louvres or transfer grilles
should be provided in walls and doors for make-up air to be introduced into
the room from its surroundings, preferably from adjacent heated corridors
(see figure 3(a)). The locations and sizes of these openings, louvres or
transfer grilles should be chosen to ensure the avoidance of discomfort due
to draughts and to ensure that the opening and closing of doors does not
affect the performance of the safety cabinets. The use of damped door
closure devices can help to reduce sudden air movement. The drawing -in
of potentially contaminated air, e.g. from adjacent laboratories, should be
avoided and the general quality of the air should be consistent with the
achievement of the environmental conditions specified for the laboratory.
Note:
Installers and users should take care not to breach the integrity of the fire
protection system particularly when fitting grilles into doors designed to
provide half hour fire resistance. Use of intumescent material is often
necessary.
4.4.5 The objective of the make-up air distribution system (and any other
mechanical ventilation system) should be to introduce the required volume of
air into the room with the minimum possible disruption to the safety cabinet
airflow pattern, and the make-up air supply system should not reduce the
degree of protection afforded to the operator by the safety cabinet. In
general, air diffusers, grilles or terminal units (whether ceiling, wall or floor
mounted) should not discharge directly towards or across the safety cabinet
aperture. It is unlikely that the room airflow pattern employed in the
performance testing procedure (see BS 5726 : Part 1) can be realized in the
majority of actual installations. However, every effort should be made to
prevent relatively high velocity air movement (velocity greater than 0.3 m/s)
anywhere in the room housing the safety cabinet (see Clark et al, 1990).
4.4.6 A fan assisted source of make-up air should be filtered, heated and
otherwise treated as necessary, to maintain the environmental conditions
specified for the laboratory. It is common practice to prevent pressurization
of the laboratory (which would cause a spread of potentially contaminated air
into other areas) by supplying a lower rate of fan-assisted make-up air than
the total extraction rate. When there is a significant reduction in or a
complete loss of the air extraction from the room by the safety cabinet
installation, the fan-assisted inflow of make-up air should be correspondingly
reduced, or disconnected, either automatically or manually following an
automatic alarm (see figure 3(b)). At containment level 3 or above the
supply and extraction airflow should be interlocked to prevent positive
pressurization of the room.
Note:
Consideration should be given to the possibility of reverse flow through a
safety cabinet when the extraction system is low or off and other safety
cabinets or containment facilities are on. Consideration should also be given
to situations where several cabinets may be operating independently in one
room.
4.4.7 If the airflow rate induced by the safety cabinet is inadequate to ventilate the
laboratory or if the laboratory is to be occupied when the safety cabinet is not
in use, separate arrangements should be made for room ventilation (see
figures 3(c) and 3(d)).
4.4.8 Air extracted by a separate ventilation system from a room in which a safety
cabinet is installed should not be recirculated.
4.5 SITE SURVEY
4.5.1 As a preliminary to the installation of safety cabinets in a laboratory the site

should be surveyed by the safety cabinet installer to assess environmental
conditions and possible exhaust ductwork route(s). The survey should
include a review of the possible positions for the safety cabinet(s) in relation
to laboratory doors and windows, the main routes of foot traffic past the front
and the effects of ventilation systems installed in the laboratory, particularly
identifying any draughts or air circulations that are liable to interfere with
cabinet performance, and confirming that there is an adequate air supply to
the room.
4.5.2 Depending on the outcome of the survey, modifications may be necessary to

improve the environment before the contractor can install a safety cabinet so
that it complies with performance requirements specified in BS 5726 : Part 3.
APPENDIX 9 - EC DECLARATION OF CONFORMITY
AND CERTIFICATE OF CONFORMITY
EC Declaration of Conformity
We the Manufacturer
Astec Microflow Ltd.
30 – 31 Lynx Crescent
Weston-Super-Mare
N. Somerset
BS24 9BP
Tel. +44 (0) 1934-410-500
Fax +44 (0) 1934 410-546
Declare that the product Biological Safety Cabinets workstation
Has been manufactured in conformity with the following standards and specifications
Electrical safety BS EN 61010-1 1993
Emissions BS EN50081-1 1992

Immunity BS EN50082-1 1992
Quality manufacturing system. ISO 9002 Certificate Number Q 7021
And Complies with the requirements of
The Low Voltage Directive 73/23/ EEC

The Electromagnetic compatibility Directive 89/336/EEC
Colin Cave
Quality Manager
16 January 2001
Astec Microflow Ltd.
30 – 31 Lynx Crescent
Weston-super-Mare
N. Somerset
BS24 9BP
Tel. +44 (0) 1934-418-685
Fax +44 (0) 1934 419-033
Certificate of Conformity
We the Manufacturer declare that the Astec Microflow range of Biological
Safety Cabinets model numbers ABS 1000 to ABS 1800 have been designed
and inspected and conform to BS EN 12469 : 2000 Class II as defined in
Section 5.4 table 4 of BS EN 12469:2000.
Colin Cave
Quality Manager
05 February 2001
APPENDIX 10 - TECHNICAL BULLETINS
Please attach Technical Bulletins to this page.

APPENDIX 11 - TEST EQUIPMENT
We recommend the use of the test equipment detailed in Table 1:
Table 1 – Recommended Test Equipment

TEST EQUPMENT MODEL SUPPLIER (WEB ADDRESS)
100 mm Vane Anemometer EDRA 6 Airflow Developments Ltd.
(http://www.airflow.co.uk)
Hot Wire Anemometer TSI 8357-GB or TA 400 Airflow Developments Ltd.
(http://www.airflow.co.uk)
Photometer JM 8000 SP101LL Steptech Instrument Services Ltd.
(http://www.steptech.co.uk)
Smoke Generator Scientific Com 10999 Steptech Instrument Services Ltd.
(http://www.steptech.co.uk)
This Appendix must be read in conjunction with the Maintenance Manual.

DOCUMENT AMENDMENT FORM
This Manual issue is an amendment of:
Advanced Bio Safety Cabinet (ABS) Operating Manual
Issue: CC/PM/02/01
Pages affected: Various see below
Description of amendment:
LOCATION DESCRIPTION
Title Page Class II inserted
Sect. 1 Figure 1 – new photograph
Sect. 3 Para 3.3; 4 th para amended
Sect. 3 Para 3.3; 6 th para amended
Sect. 4. Table1 Revised
Sect. 5 Para 5.1; (e) para amended
Sect. 6 Para 6.2.2; Procedure para amended
App. 4 Para 2.1; para amended
Issued by:
Name Date:

ABS Operating Manual Class II - RG - PM (Old)

Uploaded by

Document Information

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

ABS Operating Manual Class II - RG - PM (Old)

Uploaded by

Copyright:

Available Formats

Caution:

Please read this Manual thoroughly.

ADVANCED BIO SAFETY CABINET

part of BIOQUELL (UK) Limited

Safety Environmental Conditions

6 Decontamination of the Cabinet

8 Control System: Customisation and Calibration

9 Calibration of Airflow and Alarms

10 Additional Airflow Set Up Functions

2 UK Approved Service Companies Telephone and Fax Numbers

3 Care and Cleaning

5 Model and Specification Data

7 Protocol for Testing, Monitoring, Replacement and Disposal of Filters

9 EC Declaration of Conformity and Certificate of Conformity

Export and Agent Sales Only

WARRANTY REGISTRATION FAX

Items marked ** are relevant to our Safescope models

WARRANTY VISIT REQUEST FAX

Location of product including address and contact telephone number

Export Only UK Only

(2) GERMICIDAL LAMP. EMITS UV RADIATION 253.7 nm. EXPOSURE WILL

SAFETY ENVIRONMENTAL CONDITIONS

The cabinet is for indoor use only.

For use at altitudes up to 2000 m.

Operating temperature range of 10°C to 35°C.

For operation at a maximum relative humidity of 80% for temperatures up to 31°C,

Installation category (overvoltage category) II.

Microflow is a registered trademark of BIOQUELL plc. Microsoft (MS) is a registered

The use of laminar flow techniques to provide a clean sterile environment

The cabinets work on a recirculating principle, approximately 70% of the air

Figure 1 Laminar Flow Advanced Bio Safety Cabinet

2.1 OPERATING ENVIRONMENT

The cabinet will operate satisfactorily in environments where the ambient

The ABS cabinet has two power supply options:

The internal control system operates at 5 V.

Activation of any alarm causes an audible and visual signal.

High efficiency filtration is provided by Astec Microflow Narrow Pleated

3.1 CONTROL PANEL

Figure 2 Control Panel

3.1.1 Mains Power Indicator

3.1.2 Alarm indicator

The alarm indicator flashes whenever an alarm condition occurs.

3.1.3 Fan Control

A special requirement to meet the requirements of the ‘GS’

A special requirement to meet the requirements of the ‘GS’

Once the fan delay is activated, it can alternatively be canceled by pressing

3.1.5 Fan 100%

A special requirement to meet the requirements of the ‘GS’

3.1.6 Alarm Mute

When an alarm condition occurs, the buzzer will sound. Pressing

Correct operation of switch indicators and the audible alarm may

3.1.8 Fluorescent Lights

Pressing this switch turns on the cabinet’s fluorescent lighting.

3.1.10 Vaporisation Cycle

Pressing this switch initiates the fumigation process. See Section

As a safety feature, in series with the gas supply tap is a solenoid

3.1.12 Keylock and PIN Number Change

The keylock switch offers different operability depending on the

PIN Number Change

3.2 ADDITIONAL FEATURES

3.2.1 Automatic Air Velocity Control

3.2.2 Automatic Fan Start After Power Failure

Refer to the Supplier or the Maintenance Provider.