DELAVIDA CHRISTIAN MONTESSOR FABELLA VILLAGE, BAGUMBAYAN ROXAS, ORIENTAL MINDORO

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SCIENCE INVESTIGATORY PROJECT (SIP)

THE EFFECTIVENESS OF
GUYABANO LEAVES AS INSECT
REPELLANT
Presented by: Shawn Zen Ederly R. Adriano
) ) ) ) ) ) ) ) ) TABLE OF CONTENTS Contents
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Introduction and background

Methodology
Results
Discussion
Conclusion
Definition of terms
References
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BACKGROUND

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General Overview History

This study is conducted to Guyabano has been used as an

analyze the effectiveness of
Guyabano as an insect repellant
alternative medicine for many
centuries now. The student
believes in the medicinal factor
of guyabano thus creating this
SIP.
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01 Chapter 1

INTRODUCTION
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WHAT IS GUYABANO?
(ANONA MURICATA)

Chapter 1

01 Guyabano (soursop) is a 02 Rich in Vitamin C and fiber, it has

medicinal value in treating
tropical fruit grown in gastrointestinal issues, skin diseases,
various regions and inflammation

03 Guyabano is a word 04 Studies show that extracts

derived from the Spanish from its leaves and fruit have
word guanabana insect-repelling properties
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PROBLEM STATEMENTS
Chapter 1

01 Can guyabano extracts 02 What is the best concentration of

effectively repel guyabano extract to use as an
mosquitoes? insect repellent?

03 What types of insects are

04 How does the effectiveness of
guyabano extract compare to
they most effective other commonly used insect
against? repellents?
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HYPOTHESIS
Chapter 1
Guyabano extract will be
effective in repelling mosquitoes
and cockroaches compared to
other commonly used insect
repellents.
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02
Chapter 2

METHODOLOGY
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SUBJECT AND OBJECTIVE

Subject: Guyabano (Annona

muricata) extract as an insect Chapter 2
repellent
Objective: To test the
effectiveness of guyabano
extract as a natural insect
repellent against mosquitoes
and cockroaches
) ) ) ) ) ) ) ) ) MATERIALS
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HUMAN ARM, CAGE, MOSQUITOES

POT AND WATER
Chapter 2

GUYABANO LEAVES CLEAN CONTAINER

 PROCEDURE
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Collect clean and undamaged Guyabano leaves

Wash and chop leaves into small pieces
Boil chopped leaves in a 1:3 ratio of leaves to water Chapter 2

for 30 minutes
Strain mixture to separate liquid from plant
material
Store liquid extract in a clean container and label
with date and concentration
 PROCEDURE
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Test effectiveness using arm-in-cage method with

human subjects
Determine minimum effective concentration Chapter 2

Calculate duration of protection

Evaluate potential side effects
Analyze data to determine most effective
concentration
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03

RESULTS AND DISCUSSION

Chapter 3
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EFFECTIVENESS RESULT
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Leaves (1g) per mL (1 mL) Effectiveness

1:1 60%

1:2 80% Chapter 3

1:3 90%
 DURATION RESULT
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Leaves (1g) per mL (1 mL) Duration

1:1 2 hours of protection

1:2 3 hours of protection Chapter 3

1:3 4 hours of protection

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SUMMARY
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In summary, this study found that Guyabano leaf extract is an effective

natural insect repellent against mosquitoes and cockroaches. The results are
consistent with previous research, suggesting its potential use in the
development of natural mosquito repellents. However, the small sample size
and laboratory setting are limitations that may limit the generalizability of Chapter 3
the findings. Further research is needed to evaluate its effectiveness in real-
world conditions, including field trials and studies involving larger sample
sizes.
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04

CONCLUSION

Chapter 4
 CONCLUSION
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In conclusion, this study found that Guyabano leaf extract is an effective and
safe natural alternative to synthetic mosquito repellents. Higher
concentrations of the extract provide greater protection for longer periods of
time. The findings have important practical applications for the development
of natural mosquito repellents, particularly in areas where mosquito-borne
diseases are prevalent. Further research is needed to evaluate the extract's
effectiveness in real-world conditions, including field trials and studies Chapter 4
involving larger sample sizes. Guyabano extracts have the potential to be an
effective and safe alternative to commercial insect repellents.
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04

DEFENITION OF TERMS AND SOURCES

Chapter 5
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Guyabano (Annona muricata) - a tropical
fruit tree native to the Americas, also known
as soursop.
Insect repellent - a substance applied to skin,
clothing, or other surfaces to repel insects
and prevent bites.
Mosquito - a small, flying insect that feeds on
the blood of humans and animals, and is
known to transmit diseases such as malaria,
dengue, and Zika.
Extract - a concentrated solution obtained
by removing the active components of a
plant or other substance using a solvent.
Concentration - the amount of a substance
present in a given volume or mass of a
solution, often measured as a percentage.
Arm-in-cage method - a method of testing the
effectiveness of insect repellents in which the
subject's arm is placed in a cage containing a
known number of insects, and the number of
bites is counted over a period of time.
Skin patch test - a test in which a small amount
of a substance is applied to a small area of skin
to check for allergic reactions or other side
effects.
Efficacy - the ability of a substance to produce
a desired effect, such as repelling insects.
Generalizability - the degree to which the
findings of a study can be applied to a larger
population or to real-world conditions.
Formulation - the process of combining
different substances to create a specific
product, such as an insect repellent.

 Thank You
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