Product datasheet

Collagenase Inhibitor Assay Kit (Fluorometric)

ab211108
7 References 1 Image

Overview

Product name Collagenase Inhibitor Assay Kit (Fluorometric)

Detection method Fluorescent
Sample type Inhibitor compounds
Assay type Semi-quantitative
Product overview Collagenase Inhibitor Screening Kit (Fluorometric) (ab211108) provides a quick, sensitive and
reliable test for high-throughput screening of collagenase inhibitors. The assay uses self-
quenched BODIPY conjugate of Gelatin (Type B) as a fluorogenic substrate to monitor the activity
of Collagenase. Upon proteolytic digestion of the highly quenched BODIPY-labeled gelatin by
Collagenase, the de-quenched BODIPY yields bright green fluorescence that can be assayed
using a fluorescence microplate reader at Ex/Em = 490/520 nm (515 nm cutoff). In the presence
of a collagenase inhibitor, the gelatin is not digested, the dequenching of BODIPY does not occur
and the fluorescent signal is not produced.

This kit can be used to screen/study/characterize potential inhibitors of Collagenase.

Notes This product is manufactured by BioVision, an Abcam company and was previously called K833
Collagenase Inhibitor Screening Kit (Fluorometric). K833-100 is the same size as the 100 test
size of ab211108.
Collagenase (EC 3.4.24.3) is an enzyme in the matrix metalloproteinase (MMP) family that breaks
down collagen, assisting in degradation of the extracellular matrix, a key step in the pathogenesis
of bacteria and tumor cell invasion. Collagen is an abundant structural protein present in the
connective tissue of animals. Collagenase has been used clinically for the treatment of
Dupuytren’s contracture, an affliction characterized by a thickening of connective tissue.

Platform Microplate reader

Properties

Storage instructions Store at -20°C. Please refer to protocols.

Components 100 tests

Collagenase 1 x 30µl

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 Components 100 tests

Collagenase Assay Buffer 1 x 25ml

Collagenase Substrate (40 µg) 1 vial

Inhibitor (1,10)-Phenanthroline (400 mM) 1 x 50µl

Images

Collagenase Inhibitor Screening Kit (ab211108). Typical inhibition

curve of Collagenase activity by collagenase inhibitor. IC50 of
(1,10)-Phenanthroline was determine to be 110.5 µM. Assay was
performed following the kit protocol.

Collagenase Inhibitor Screening Kit (Fluorometric)

(ab211108)

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