‘Trarbng DH Cong Nghé Sai Gon Lop: CNTPO8.4 TH Phan Tieh Thye Pham Nhom:30 BAL 3: Nac djnh him lugng tink bot bang phureng phip hd hge Tinh bot la thanh phan cha yéu cia nhiéu logi cut va hat, vige xée dinh ding sé gidp ta dy kién ehinh xéc krong sin phim thu dueoe cing nhur tn that trong qué trinh san xuat Phuomg php xac dinh ham hong tinh bot ¢6 nhiéw nineng cé thé chia lam 3 nhém: Phuong phap quang hoe: dima vio kha ning lim quay mit phing anh sing phan ee cia tinh bor Phuong phap hé hoe: dua trén co s¢ thuy phan tinh bot dén glucose biing anid, sau dé xde inh kha nding khir cua dung dich. ‘ Phuong phap sinh hee: dua t1én eo $6 thay phan tinh bdt bing amylaza, sau d6 dem Ken men dich dong dé bién thanh rugu roi xe dinh long negu tao thin, 1. Nguyén ti ‘Thuy phan tinh St thanh dvong trong dung dich HCI 5% 6 digu kign dung s6i trong binh céch thuy trong thei gian 1 gid. Dung dich sau thay phin duge im ngudi va trung ho bing NaQH vi chi thj methyl da cam. — Ham fugng dung trong dung dich sau thuy phn c6 thé duge xée dinh bing nhigu huang phip, tuy nhién (rong phgm vi bai thi nghigm nay ham Iugng duimg due sie dink bing phuarg phap Ién miu v6i Ferrycyanure. qui lung dirimg khit trong dung dich sau thuy phn trt di hrong dung Khir trong dung dich trude thuy phiin ehinb & lugng dudng hinh thanh tir qud trink thuy phn tinh bot. Hi sé my hin vii hé 58 chuyén d&i dung khit (glucose) thanh tinh bgt li 0.9 ta s& o8 durge him Iugng tinh bot trong miu nguyen Tigu ban cu TI. Dung cy — 6 chat L. Dung Can phan tich ¢6 d9 chinh xac 0.001g Nnigt ké do duge dén 10°C + Binh dinh mite dung tich 100, 250m1 + Binh tam gic dung tich 250m! = Céc thay tinh 100, 250ml = PhEu th tin + Ong dong dung tich 100m! # Ong sinh han khi © Noi cach thuy + Bop ign 2. Ho eh Axit chlohidrie dae Dung dich Ferrycyanure 1% Dung dich glucose 0.5% Dung dich hyciroxyl natri 20% (SN) Dung dich hycroxyl natri 10% (2.5N)‘Trarbng DH Cong Nghé Sai Gon Lop: CNTPO8.4 TH Phan Tieh Thye Pham Nhom:30 Methyl da cam (CHsCOO);Pb 10% NazHPOs bio ho Methylene Blue IIL Cach tiém hank thir x4e dinh him long dudng khit ban dau trong mau: sir chiah xac 5.01g bot, to}n voi 30ml nude edt, chuyén tn bo hn hop vio bin tam gide 100ml, Sau 46, két tia protein va céc tap ebét bing dung dich acetate chi 10%(2 — $ ml) hode CHCI, 5%, sew 46 loai, bo lugng acetate chi ci bing dung dich Na:HPO, ‘bao bo (3 5 ml) then vor lgng vita cu de két tua bon tén acetate chi de, : Bé yén hén hop trong 10 phir, chuyén vio binh dinh mite 100ml, tring kg, thm nurée edt t6i veh dinh mife va dem loc, ‘Nude lge duye mang di chun d6 dung dich Ferryeyanure 1% 5.01 g bt gn véi ‘nase eke 1B Cho dung dich acetate Cho dung dich 30m nude edt 281 i chuyéntointg hin | C—>> | chi 10%2—Sml | => | NasHPO, bao hap vo binh tam hole CHCh 5% hoa (3-5 ml ‘giée 100ml ‘Dem di chuan do dung. 1 Dé yén 10 phiit, tién han dich Feryeyamure 1% | || > | anh mre trong binh din mite 100ml 2. Chudin bj mau thir xée dink ham twgng tinh bot: ‘Can 2.00g 661 roi chuyén tin bO vao binh tam gic 250ml. Tip theo cho thém 100ml HCI 5%, nay niip lai. Lae nhg rdi dgt vie néi dun cdch thuy, dun tdi sdi va cho sdi Khoding 1 ai. Mire nuzéc 6 ndi each thuy phai Iuén cao hon mire suc trong bink thuy phi, phai chudn bj mde s6i dé bd sung vo, Sau I gid thuy pian, tin 6% lugng tinh bO« da chayén hd thanh glucose, hong rOi cho thém 4 ~ 5 gigt methyl da earn, ding NaOH 20% dé trang mtu (tir hong chuyén sang vang) ; Chil Y= Chi trung hd khi da Him ngudi dén 30°C vi Gr nhigt dd cao vA kiém eye bd «hl glucose s® bj phan huy Lim két qua kém chinh xc. “Trung hd xong ta chuyén tén b} dung dich vio binh dial nnke 250m, tring ky, thém nude edt ti vach dink mite, bie KF va denn loc‘Trarbng DH Cong Nghé Sai Gon Lop: CNTPO8.4 TH Phan Tieh Thye Pham Nhom:30 Dich dung thu duye sau khi lgc mang di chuéin ep dung dich Fesryeyanure 1% 2.00 g hor Cho 100ml dung Bun céch thny (dun wong binh am | [——T> | dich HC1S% dy | (| soi eho s0iT git) 501 ev nip, He nhe Chun d9 voi NaOH dinh mire 20% (mau hong Lam ngudi, cl r 2% ni, cho 5 seen | <5) save <> | siotmenyt aa cam som ‘Dem di chudn d dung toe] => | ck Femeyanar 6 3. Tién hank chun a6: Cho vao binh nbn 1Oml dung dich KsFe(CNJe 1% vi 2.5 dung dich NaOH 2.5N, them vio 1 gigt Methylene Blue. ‘Dun s6i va chun d6 ngay trén bép bing dung dich duéng khir hode dich duong sau thay phan ti burette, cho time giot, lic manh. ‘Dung dich s2 chuyén tir do sang ving vi mQt eigt dung thi diu tien se lam mét mau xanh Methylene Blue cho biét phan img ket thie. ‘Sau Kin chudn dQ du tién mang tinh dink hudng dy, tién hanh ehudn do in thir 2. Lin ny sau Khi dun s6i dung dich Ferryeyanure, xa nharh long durong (theo Két qué in chun 49 truée ), chi dé lai duéi Imi d8 chuan dd tiép tim chinh xe diém e: Két qua tinh ton chi sir dung tir lan ehudn 46 thit 2 tre di. Lap lai th nghigm 3 tin 4, Xe djnh leyng durong glucose chusin 0.5% tiéu cn dé phan dng hdc v6 10m dung dich KFe(CN)e 1% Chuan 46 tuong ty nhur di veri dung dich dung Khir nfung thay dung dich dutmg kin irén bureite bing dung dich durimg glucose chun 0.5%. Lap lai thf nghigm 3 kan.‘Trarbng DH Cong Nghé Sai Gon Lop: CNTPO8.4 TH Phan Tieh Thye Pham Nhom:30 Chi §: Voi nhiing dung dich dudme khong wu hoe co mau nyt, ta 66 thé King cho chi thj Methylene Blue, chi xic dnh dim cud khi miu cia dung dich chuyén ti mau ving dm (Ferrycyanure) sang mau vang rat nht (Ferrocyanure). Binh nén: ~ 10ml KeFe(CN)s 1% |), | Bum sBi, chun d9 ngay wren bép = 2.5ml NaOH 2.5N {> | saing dung dich durong ki, dich = T gigt Methylene Blue durong sa tuuy phin hoae dung dich duémg ghicose chin 0.59% 1V. Tinh két qua: ‘The tich dung dich glucose 0.5% ding cho chuan 46 (V,) .60ra .60ml 70m 0m 2.6426+2.7 Vee= = 2.63m1 ‘Thé tich dung dich dubng khir ding chudn a6 (Vx), Ve = 134ml Vig = 13.5ml Vi = 13.5ml Vs = 134m Veo BAHBS# 13S 15 gmt ‘Thé tich dung djch dudng sau thuy phan ding chudin 49 (V1) Veo1 = 2.00ml Virp2 = 1.90 Very: = 1.60m Vires=1,90ml Virw A 2419419 gay 3 ‘Ham long dug khir trong nguyén ligu: Xu= WS ey,e 100,‘Trarbng DH Cong Nghé Sai Gon Lop: CNTPO8.4 TH Phan Tieh Thye Pham Nhom:30 05 yy gu 100 4100 13150 ge 100"? "3.47 "sor or4sa7 18S #1008 2X Largng durdng khit ban di (g/1 00g) Vo: The tich dung dich glucose 0.5% ding cho efi 46 (ml) Vie: The tich dung dich duximg hte ding chusin d6 (ml) Ve The tich binh dinh mie dong kh (ml) m: Khéi hrgng mu thi nghism (g) Ham long duéng khit trong dich thuy phan: ° 100 Kin 2S eye Yo 100 7100! Vm : = 95 ap 654250 100 _ 32875 _ 95 rye t00g i00 "93" 2 ~ 386 Xqp: Luyng dung khir trong dich thay phin (g/1002) Vg: Thé tich dung dich glucose 0.5% ding cho chuain 49 (il) Vio: Thé tich dung dich during sau thuy phn ding chuain a (ml) ‘V: Thétich binh dinh mirc (ml) rm: Khoi fugng mi thi nghiém (g) Ham hrgng tink bt trong ma X= (Xyp— Xe) "09 = (85.17 — 1.95)* 0.9 = 74.98/1008, X: lrgng tinh bot c@ trong mau ban dau (2/1003) 0.9; hg so chayén ho glucose thin tinh bot V. Bign Iujin va gia thieh: 1. Bign wan: hin vao két gua chun d9 bing 2 logi dung dich: Logi I: dung dich dung kha. ‘Logi 2: dung dich dudng sau thily phn ‘Ta thy: thé tich dung dich duing khir tiéu t6n che qué trink cht so vi the tich dung dich dudmg sau thy phi efing tu i6n cho qué tinh chusin dQ nbur tren. K&L qui chuan a ‘Thé tich dung dich duing Khir dig chudn 49 (V4) Vay = 134ml Viz = 13.5ml Vis = 13.5ml‘Trarbng DH Cong Nghé Sai Gon Lop: CNTPO8.4 TH Phan Tieh Thye Pham Nhom:30 Vir = 134ml ‘Thé tich dung dich duing sau thuy phiin ding chuan a6 (Ve) Veer = 2.00mi Vire2 = 1.90m Vis 160ml Vies= 1,90m Két qua chuan 40 bing 2 logi dung dich trén e3 sy chénh Igch cao niu vay la do trong dang dich duimg khir ¢6 him lugng duéng thap nén sy tiga t6n thé tich sé nhiéu, cOn trong dung dich dung sau thy phan e6 him lung dug cao nén sy tigu 16n sBithon tir day ta c6 thé két ign néu dung dich chusin d9 c6 him lkeng ducing e: thé tich vigu tn sit di, Con dung dich chun d6 e6 ham hung durdng,thép thi thé tich tigu ton se anhigu hen. VL. Cae phrong phaip khic: . Phuong phap hoa hye: xée dinh bing eich do curing d6 m bing céch dinh lngng glucose tgo thanh sau khi thiy phan tink bOt bing axit hog amylase 1 cia tinh bot vGi Tot hoge ing enzyme (Cach tién hank: Can 200 — 250 me tinh bt cho yao binh cu dung tich 100 ml. Cho thém vdo binh 50 ml ude eat, lic déu, dé yén 30 ~ 45 plnit i dem loc dé loai bo during tan, Rita tinh bét bang mde edt 2-3 lan, Choe thung gidy loc va chuyén tinh bét vao binh cna chita 25 ml dung dich H1CI5%, Déy kin binh bing mit cao su. . Dun cach thay hén hop 3 ~ 5 gio, Thr su thuy phan lén ton tinh bat bing dung dich lot, Lam nguéi, trung hoa hdn hop bang dung dich NaOH 5% dén pH = 5.6 6, Chuyén hén hop vio binh dinh mite 100 ml, ket tia protein bing acetate chi 10%. Loai bo acetate chi dur bang dung dich Na:HPOs bio hi, Cho nude edt dinh mite t6i vach, Tién hanh hoe. 5 ml bode 10 ml) bing phuons php Binh Iugng dudng glucose trong dung dich Beetrand, (Cong thie tink him lwyng tinh xn atl *100%0.9 vow XX: Hm rong tinh bot (%) a; 6 me glucose, tra bain tinh duge (img vei sé ml KMnO,) ding chuan d6 mau thi nghigm trir di s¢ ral KMnO, ding chuan d> miu chung véi ching ‘Vr: thé tich dang dich dmg (san thiy phan) lay dé xe dinh during glucose (rl) tieh binh dink mite‘Trarbng DH Cong Nghé Sai Gon Lop: CNTPO8.4 TH Phan Tieh Thye Pham Nhom:30 100: hg sb chuyén thanh % Phurong phap eye pho Phuong phap khiic xa ké Piuzong phap héa sinks Vil. Ching minh e6ng thite: Ham rong duéng kinr trong nguyén tigu: 1OmI KsFe(CN)o E> Ve rene 0.5% YOm KsFe(CN)g ny Vie X% Vet 0.5% = Vig? X% VeFK% 100 (trong 100 ml dung dich) Diph mite 100 ml nén ta 5 eding thie: as v 100 eg ME im iam lrgng during sau thay phan: HOI KsFe(CN)¢ E> Vg ane 58% 10m K:Fe(CN)s Vie X% Ve 0.5% = Vert X% 0540, Vip X% 100 100 Grong 250 mil dung dich) Dinh mite 250 ml nén ta 6 cng thite: Ham Iugng tinh b9t trong miu: Tinh bot (CaHio0s}s-——=—> 0G Lap ti so giita tinh bot va dung ta 66;‘Trrmg DH Cong Nghé Sal Gon Lop: CNTPO8.A TH Phin Tien Thye Phim Nhom:30 reno? PD Hes6 wO9