Early Human Embryonic Development

Lab
 Lab 1
Formation of germ layers and related structures
ไ ง ตน
1. Study total mount of chick embryo at primitive
streak stage ~
hdmdh
Wd ขอบ

2. Study total mount of chick embryo at notochord

(head process) stage
เ ยบ
3. Study serial transverse sections of chick embryo
at primitive streak stage
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 Primitive streak stage
เ ฟะ 401K บน
dorsdl surfdcl ขอ VEM µ 0
Area opaca
L
R ใสก า ะ
เ า Embrlp
Area pellucida

Primitive node
/
ห ม
Primitive pit

นเ าV
Primitive fold
Primitive streak
วม
Primitive groove

Courtesy of Assoc. Prof. Dr. Jantima Roongruangchai, course material of MSc class
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 ก ม rell นWทห า

Notochord (head process) stage

Head fold วใ เ น

Notochord
"
"" "

Primitive node

Primitive pit
Area opaca
Area pellucida
Primitive fold
Primitive streak
Primitive groove

Courtesy of Assoc. Prof. Dr. Jantima Roongruangchai, course material of MSc class
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 Notochord (head process) stage
เห ยว ใ เ ด neuvdlpldtl
generdlblastdlrm
Proamnion cmelodlrm
ไป ไ ป 1
Head fold
Margin of mesoderm
Neural plate neural fold

% ส ฟ Notochord
Primitive node
Primitive pit

Area opaca
Area pellucida
Primitive fold
Primitive streak
Primitive groove
Blood island

Courtesy of Assoc. Prof. Dr. Jantima Roongruangchai, course material of MSc class
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Serial transverse sections at
primitive streak stage

See VDO clip: animal embryonic section

(VDO period = 0:00-16:45 min.)
 Lab 2 Edtly 4
Somite stage of chick embryo: part 1
021 ปลาย W}

1. Study total mount of chick embryo with 3-5

somites
2. Study serial transverse sections of chick embryo
with 3-5 somites
 Embryonic stage having 3-5 somites
ด neuraltuty Anterior neuropore
งไ
#
ใน หอ
4 Head ectoderm
¢ ✗
µ
พบ Head mesenchyme
\
๓
Foregut (wall) รอบ อ midgut Foremt
-

Anterior intestinal portal

Midgut (area)
8 somite
ก า
Neural fold
85 ๆ from neural plate
8
Neural groove
Somite

อ
Notochord
Primitive node
Primitive fold
จาก ตอน แรก Primitive streak
เพราะ า ขา
Primitive groove
เจ ญ เยอะ Area opaca
Area pellucida
Courtesy of Assoc. Prof. Dr. Jantima Roongruangchai, course material of MSc class Blood island
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 Serial transverse sections at
embryonic stage having 3-5 somites
Study the region of head which is free from general
blastoderm
Study the cephalic region of midgut
Study sections through somites

See VDO clip: animal embryonic section

(VDO period = 16:45-33:00 min.)
 Lab 3
Somite stage of chick embryo: part 2
0แ

1. Study total mount of chick embryo with 7-10

somites
2. Study serial transverse sections of chick embryo
with 7-10 somites
 Embryonic stage having 7-10 somites

C) ตา เ ด

)
กระเปาะ
)

7 somites 10 somites >10 somites

Courtesy of Assoc. Prof. Dr. Jantima Roongruangchai, course material of MSc class
ป็
Embryonic stage having 7-10 somites
fonetrain
Prosencephalon

Optic vesicles
midbrain
Mesencephalon
Foregut (wall)
hindbrain
Rhombencephalon
Heart
Anterior intestinal portal

Vitelline vein

Midgut (area)
Somite

Spinal cord
Courtesy of Assoc. Prof. Dr. Jantima Roongruangchai, course material of MSc class
Embryonic stage having 7-10 somites
>10 somites

hedrt

https://www.vcbio.science.ru.nl/en/virtuallessons/embryology/chicken-33-36h/
 Embryonic stage having 7-10 somites
Somite

| เ น กระบอก
Spinal cord

} า าง

Neural fold
Notochord

Primitive node

Primitive streak

Courtesy of Assoc. Prof. Dr. Jantima Roongruangchai, course material of MSc class
ห่
ผ้
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 Serial transverse sections at
embryonic stage having 7-10 somites
Study the cephalic region
Study the region caudal to anterior intestinal portal

See VDO clip: animal embryonic section

(VDO period = 33:00-52:00 min.)