The Effects of Aloe Vera Clinical Trials on Prevention and Healing of Skin

Wounds

Kenth Robert T. Magallon

Pascual H. Magan
Christine T. Millones
Cherilyn Lusbong
Elmira Magsalay

March 2023
 INTRODUCTION

Background of the Study

Aloe vera (Aloe vera Linn, synonym: aloe vera barbadensis Mill.) is in

family Liliaceae, which is a tropical plant easily grown in hot and dry climates

including Thailand. Numerous cosmetics and medicinal products are made

from the mucilaginous tissue, called aloe vera gel, located in the center of the

aloe vera leaf. Aloe vera gel has been used for many indications since the

Roman era or even long before. Burn wound healing is one of major indications

of aloe vera gel use in many countries (E.I. Helvig 2022).

Clinical multiple acute trauma has brought great suffering for patients.

The use of traditional Chinese medicine provides a new approach to treating

trauma. Aloe Vera gel has been used to treat skin diseases for a long time, but

its efficacy is not stable. As the most important part of the organic active

component of Aloe Vera gel, the effect of aloin in acute trauma has not been

reported. In this article, the effect of aloin on promoting wound healing was

investigated through a series of experiments in vitro and vivo. For the in vitro

experiment, 3-(4,5 -dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cell

proliferation and cycle analysis demonstrated that aloin could enhance the

proliferation of human skin fibroblasts and endothelial cells. Transwell and

scratch tests demonstrated that aloin could increase the chemotaxis and
migration on human skin fibroblasts, and the tube formation assay

demonstrated that aloin solution significantly increased the tube network

formation compared to the control group (Hormozi, M., Assaei, R., & Boroujeni,

M. B. (2017).

Therefore, the process of wound healing is a complex biological process

and promotion of tissue recovery is the main objective of medical interventions.

Skin lesions are caused due to different reasons such as burns, arterial

diseases, surgery, and trauma (Dat AD, Poon F, Pham KB, Doust J., 2012).

Wound healing is a dynamic process that takes place in three phases. The first

phase is inflammation, congestion, and leukocyte infiltration. The second

phase involves the removal of dead tissue and the third phase of proliferation

includes epithelial regeneration and fibrous tissue formation (Reddy CU, Reddy

KS, Reddy JJ. 2011).

Moreover, the regenerative properties are due to the compound

glucomannan, which is rich with polysaccharides like mannose. Glucomannan

affects fibroblast growth factor receptors and stimulates their activity and

proliferation, which in turn increases the production of collagen. Aloe vera gel

can not only increase the amount of collagen in wounds but also change the

composition of collagen, increase collagen cross-linking and thereby promote

wound healing.

Considering the availability of several clinical trials on the effect of Aloe

vera on the prevention and healing of skin wounds, as well as its popularity
among people and widespread use in the cosmetic industry, the present study

aimed to review research studies on this topic.

This study shows the effectiveness of aloe vera in healing different kinds

of wounds.

Objectives of the Study

Generally, it is conducted to produce a product out of the Aloe Vera

leaves in healing skin wounds.

This aims to:

1. Investigate the effects of aloe vera in different types of skin

wounds,

2. Examine the safety and efficacy of Aloe Vera in treating skin

wounds.

Significance of the Study

This study is beneficial to the following:

Community. It helps to improve the skin health of many individuals and can

be used to treat minor cuts and burns. And it can be used as a natural

moisturizer helping to reduce dryness and restore skin elasticity.

Parents. It gives an idea for them not to use chemical drugs but instead of

using aloe vera as herbal medicine. It can be use as first aid medicine for the

skin wounds.

Future Researchers. The research conducted on aloe vera can help future

researchers in several ways. It can provide a better understanding of the

therapeutic properties of aloe vera, including its potential to treat a variety of

skin conditions. It can also provide insight into the potential for aloe vera to be

used as a natural supplement for general health and wellness.

Scope and Delimitation

This study aims to determine the effectiveness of aloe vera in dealing

skin problems healing in random individuals.

Time and Place of the Study

This study will be conducted at the residence Kenth Robert Magallon in

purok Hillside, Sta Cruz, Rosario, Agusan del Sur on March 2023.

Operational and Definition of Terms

Aloe Vera (Aloe barbadensis miller) is a succulent plant used in alternative

medicine. With a total of 420 various plant species of Aloe vera, it has been

popular in Indian medicine for the treatment of many conditions.

Clinical Trials controlled experiments that are conducted to test the safety and

efficacy of a new drug, device, or medical treatment.

Prevention the act of preventing something from happening or occurring.

Healing the process of making or becoming sound or healthy again.

Skin Wounds an injury to the skin, such as a cut, scrape, or burn, that

includes both the epidermis and dermis layers of the skin.

Conceptual Framework

INPUT PROCESS OUTPUT

 Aloe Vera  assessment of  The Effects of Aloe

aloe vera extract Vera in Clinical
as a healing Trials on
product at Sta Prevention and
Cruz, Rosario, healing of Skin
Agusan del Sur. Wounds.

Figure 1. Schematic Diagram of the Study

Figure 1 above shows the conceptual framework of the study. The

input of this study is the aloe vera. On the other hand, the process is the

assessment of aloe vera extract as healing product at Purok Hillside Sta. Ceuz,

Rosario Agusan del Sur. And the output of this study is the effects of aloe vera

in clinical trials on prevention and healing of skin wounds.

 REVIEW OF RELATED LITERATURE

The review of the study focused on the experimental research of aloe vera

clinical trials on prevention and healing of skin wounds. Facts and information

were gathered from the books and internet as related studies in order to get the

needed information for the study.

Characteristics of aloe vera healing effects and its benefits

Skin serves as a barrier to water and various pathogens. Wounds and

injuries destroy this barrier that normally prevents invasion of bacteria, fungi and

viruses. Wound healing is a complex and dynamic process of restoring cellular

structures and tissue layers. The human adult wound healing process can be

divided into 3 distinct phases: the inflammatory phase, the proliferative phase,

and the remodeling phase. Within these 3 broad phases there exist a complex and

coordinated series of events that includes chemotaxis, phagocytosis,

neocollagenesis, collagen degradation, and collagen remodeling. In addition,

angiogenesis, epithelization, and the production of new glycosaminoglycans

(GAGs) and proteoglycans are vital to the wound healing milieu. The culmination

of these biological processes results in the replacement of normal skin structures

with fibroblastic mediated scar tissue (Kumari S, Harjai K, Topical C., 2010).

Medicinal properties of Aloe vera range from external burn treatments to

helping relieve constipation by consumption. Aloe vera is the most efficacious

natural plant used both externally and internally and there are numerous benefits
that are derived from this wonderful plant. The health benefits of Aloe vera have

been propagated throughout the world. There are two parts of Aloe vera that are

commonly used. The bitter exudate is used as a natural drug for its cathartic

effect and is widely employed as a bittering agent in alcoholic beverages and as a

laxative. The dried latex, with a high barbaloin content, is a strong laxative, but its

use should be limited to no more than one week, and it should not be used during

menstruation, pregnancy and nursing. The suggested medicinal use of Aloe vera is

based on its historic and traditional use, and an analysis of modern

pharmacological and toxicological research. The inner gel, or “pure gel”, is the

more readily known part of the Aloe vera plant. This is the section of the plant

that is most commonly known to be used for treatments of sun burns. But if

prepared properly, the inner gel can be consumed to help treat certain internal

ailments (Manvitha K, Bidya B., 2014).

Aloe vera is a great example of functional food that plays a significant

role in protection against oxidative stress (Joseph B, Justin Raj S., 2010). Its

action is related to the reduction of the process of cell destruction during

stress, and also minimizes biochemical and physiological changes in the body.

The antioxidative action of aloe vera gel extracts made in methanol, 95%

ethanol, hexane, acetone and chloroform were tested in vitro. The results

showed that methanol and acetone extracts had the maximum capture of

DPPH free radicals and superoxide radicals. All extracts were effective in the

replacement of hydroxyl radicals in a non-ion specific test as well as in a place-

specific assay. It was also found that the formation of ferrozinium-

Fe2+complex is 1426incomplete in the presence of methanol and acetone,

which indicates their ability to chelate iron. Similar conclusions were

observed in research conducted by Rahmani and Athiban. Moniruzzaman

reported that ethanol skin extracts of aloe vera contain the highest phenol

content and flavonoid content, which is clearlyconfirms high DPPH scavenging

activities and FRAP values and indicates the potential use of this plant

as an antioxidant.The antioxidant properties of aloeverawere also foundin

rodent model studies, which were fed regularly with plant leaves. The

results obtained in this experiment showed an increased activity of

antioxidant enzymes and a reduced content of lipid peroxidation products in

animal tissues ( Bawankar R., Singh P., Subramanian B., 2014).

Aloe vera is anthelmintic, aperients, carminative, deob-struent,

depurative, diuretic, stomachic and emmena-gogue. Juice is used in skin care

medicine, dyspepsia, amenorrhea, burns, colic, hyperadenosis, hepatopathy,

Aloe vera is anthelmintic, aperients, carminative, deob-struent, depurative,

diuretic, stomachic and emmena-gogue. Juice is used in skin care medicine,

dyspepsia, amenorrhea, burns, colic, hyperadenosis, hepatopathy,

Aloe vera is anthelmintic, aperients, carminative, deob-struent, depurative,

diuretic, stomachic and emmena-gogue. Juice is used in skin care medicine,

dyspepsia, amenorrhea, burns, colic, hyperadenosis, hepatopathy,

Aloe vera is anthelmintic, aperients, carminative, deob-struent, depurative,

diuretic, stomachic and emmena-gogue. Juice is used in skin care medicine,

dyspepsia, amenorrhea, burns, colic, hyperadenosis, hepatopathy, Aloe vera is

anthelmintic, aperients, carminative, deob-struent, depurative, diuretic,

stomachic and emmena-gogue. Juice is used in skin care medicine, dyspepsia,

amenorrhea, burns, colic, hyperadenosis, hepatopathy, V Aloe vera is

anthelmintic, aperients, carminative, deob-struent, depurative, diuretic,

stomachic and emmena-gogue. Juice is used in skin care medicine, dyspepsia,

amenorrhea, burns, colic, hyperadenosis, hepatopathy (Cieślik E, Turcza K.

2017).
 METHODOLOGY

Preparation of Plant Sample

The Aloe Vera (barbadensis Mill) is collected freshly from Purok Hillside,

Sta Cruz, Rosario, Agusan del Sur. The collected leaves of the aloe vera were

washed with cold water and place in a room temperature. After in a few

minutes of storing the leaves prepare a clean container with cover to help

preserve the product. In processing we used blender to refine the pulp of the

aloe for best results.

THE PROCESS OF MAKING GEL IN ALOE VERA

Firstly, slice off the outer leaf of a fresh aloe plant. The outer leaves are

more likely to be mature and contain plenty of fresh, healthy gel. Select leaves

around the base of the plant, with healthy green, rigid stalks growing close to

the dirt. Use a sharp knife or clean pair of scissors to make a neat cut as near

to the base of the leaf as you can. Secondly, rinse the leaf under cold water and

cut away any yellow gel. Check the bottom of the leaf where you made the cut

and trim away any portions that display a yellow or discolored discharge (in

other words, any substance that isn’t the transparent aloe gel). This discharge

contains latex, which may cause allergic reactions in some people. Thirdly, peel

the leaves with a vegetable peeler or knife use a vegetable peeler to carefully

peel away the green skin of the leaves. Be sure to remove the thorns and cut

through the inner white layer to the gel underneath. Peel of all of the skin on

one side of each leaf, leaving a canoe-shaped half filled with gel. Fourthly scoop

the gel out with a spoon or knife, the clear, soft gel is easy to scrape away from
the skin. Use one hand to hold the leaf in place, and use the other to scoop it

all out into a clean bowl until there’s no more gel remaining in the leaf. And

lastly mix the aloe vera gel in a blender freshly harvested aloe often has clumps

or is even congealed into a strip, which makes it difficult to apply. To give your

aloe a smoother consistency, place it in a blender and gradually pulse it on a

low setting until its smooth and easily poured.

COLLECTION OF ALOE VERA

First, cut a mature leaf from plant, choose a thick, mature leaf from

plant and use a sharp knife or garden shears to cut it at the plant’s base.

Secondly, slice the leaf in half lengthwise, cut along the aloe leaf’s length to

reveal the thick green or clear gel inside. Thirdly, squeeze the gel out, squeeze

the leaf over a bowl forcing the aloe gel out of the leaf and into your container.

And lastly, you can store aloe vera in an airtight container in the refrigerator

for up to 7 days. You can also place aloe vera in an ice cube tray and freeze it

for up to year.
Figure 2. Actual photo showing the location where the aloe vera collected from
Purok Hillside, Sta. Cruz Rosario Agusan del Sur.

A. PREPARETION PLANT SAMPLE

The Aloe Vera (barbadensis Mill) is collected freshly from Purok Hillside,

Sta Cruz, Rosario, Agusan del Sur. The collected leaves of the aloe vera were

washed with cold water and place in a room temperature. After in a few

minutes of storing the leaves prepare a clean container with cover to help
preserve the product. In processing we used blender to refine the pulp of the

aloe for best results.

B. HOW TO EXRACT ALOE VERA GEL FROM A LEAF

You can extract aloe vera gel from a leaf by squeezing it out (if

smaller), by cutting the skin off, or by removing the flat outer layer

and the spooning out the flesh.

C. PREPARETION OF THE ALOE VERA INTO GEL

Once the leaf has been peeled, you will see natural aloe vera gel.

Using a small spoon, scoop it into your blender. Be careful not to include

any pieces of the aloe vera skin. Blend the gel until it’s frothy and

liquefied, which should only take a few seconds.

D. WHAT TOOLS AND EQUPMENT NEEDED

You only need a few things from your kitchen to make homemade

aloe vera gel, gather these items ahead of time to speed up the process.

 Sharp knife

 Cutting board

 Blender

 Small 4 oz jar

 Cup or vase

 Paper towels

 Disposable gloves (optional)

 Spoon (optional)
 E. STERILIZATION OF LABORATORY MATERIALS

The laboratory materials were sterilized before and after

experimentation to avoid contamination pf bacteria in the working

place. The materials were place in the autoclavable bag and were

sterilized in the autoclave at 249.8 degrees Fahrenheit at 15 psi.

PROCEDURE IN MAKING ALOE VERA GEL

the raw material in making the aloe vera gel are an aloe vera leaf, a knife

or vegetable peeler, a small spoon, a blender, an airtight container for storage,

powdered vitamins C and vitamin E (optional). Once you have gathered all of

the material you need, it only tales about 1 hour to make your aloe vera gel.

Prepare the aloe vera leaves, to use a fresh aloe leaf from a plant, first cut off

one of the outer leaves from the base of the plant wash it well, removing any

dirt, and then stand it upright in a cup of a bowl for 10-15 minutes. This

allows the yellow-tinted resin to drain out of the leaf.

To make the gel, once the leaf has been peeled, you will see the natural

aloe vera gel, using a small spoon, scoop it into your blender. Be careful not to

include any pieces of the aloe vera skin. Blend the gel until its frothy and

liquefied, which should only take a few seconds. At this point, your gel is ready
to use. However, if you plan on keeping it for more than 1 week, you should

add preservatives.

DECONTAMINATION PROCESS

The materials used during the experiment were decontaminated, were

wrapped, and were placed into the autoclavable bag. The bag was placed inside

the autoclave for 25 minutes and the materials were washed thoroughly after.

SUMMARY

Aloe vera gel is a natural product made from the leaves of the aloe vera

plant, which is known for its many beneficial properties. It is widely used in

many skin wounds and hair care products due to its ability to soothe,

moisturize, and heal dry skin and scalp. Aloe vera gel is also contains

antioxidants, vitamins, and minerals that can help to reduce inflammation,

improve skin elasticity, and promote cell generation. Additionally, it can be

used to help treat sunburn, acne and other skin condition.

CONCLUSION

The study revealed that the research was conducted at Purok Hillside

Sta. Cruz, Rosario Agusan del Sur. The researcher used experimental design
and have utilized experimentation of aloe vera leaves, and observation to

accomplish of extracting an Effects of Aloe Vera Clinical Trials on Prevention

and Healing of Skin Wounds. Which experiment was done at residence of Mr.

kenth Robert T. Magallon at Rosario of the availability of materials. The

conclusion of aloe vera gel is that it is an effective natural remedy for a variety

of skin conditions, and can be used to soothe burns, reduce inflammation, and

moisturize the skin. It is also believed to have antioxidant and antibacterial

properties, making it a great choice for treating acne and other skin issues.

RECOMMENDATION

The experimented Aloe Vera as a healing wound gel are found effective.

Based on the study of aloe vera benefits, 10-20ml per day of aloe vera is

enough to help the wound to recover and heal. This is the range that appears

to maximize health benefits while minimizing adverse effects. The result of the

study is strongly recommended as it pass to the clinical trials with the concrete

results. To the future researchers, this research is highly recommended to have

a further and deeper study to know the other benefits and characteristics of

the aloe vera plant asides in healing wounds.

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