Plan

Biblio
-burns type
-skin damage
-treatment
-alternative: cell therapy
1-MSC :
*Source
*Caractéristique
2- EV :
*structure, origine
*characteristic
-Conditions de culture de MSC (Mesenchymal stem cells)
1- Utilization of FBS
2- Cons of FBS
3-Utilization de peptone
*Nature of peptone
*characteristic
* Effect
Introduction

Cell therapy leads to reduce the healthcare cost by decreasing the need for
long-term treatment, which can be expensive (Greenwood) 1
Besides, it became a very useful way to treat different illness, due to its
advantages like the low risk of cell rejection, this can involve the use of stem
cells, which have the ability to differentiate into different cell types and can
potentially regenerate damaged tissue.
In the other hand, cell therapy includes the extracellular vesicles, which are one
of the cell's communication mechanisms, they are produced by different type
of cells like dendritic cells, B lymphocytes and Mesenchymal stem cells
(Keshtkar, Somayeh, et al ).2
EVs have the potential to be used as a therapeutic agent, they participate in
tissue regeneration by transfer the information exists in the proteins and other
biologically important molecules to the damaged cells, also they increase the
expression of antiapoptitic genes such as BC-xL and BCL2, and decrease the
expression of proapoptosis gene such as caspas. (Keshtkar, Somayeh, et al) 2.
“Bruno and Al” improves that EV were the method that MSC improved tubular
injury and renal function. (Bruno, S., Grange, C)4.
To produce EVs, biologists use SVF to amplify the MSC, which is so expensive,
and it can be a source of contamination, that is why our project aims to
optimize the production of EVs by adding vegetal Peptone to the cell culture.
This peptone has the ability to help the cells to attach to the surface, which
means less use of SVF.