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Group Members :

 Nouman Ejaz
 Ali Haider
 M.Luqman
 M.Hammad
 Suneel Asghar
 M.Usama
 Zeeshan Arshad

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Biosafety Risk
 Biosafety risks are defined as the
likelihood of infection by the agent and
likelihood of exposure through the
infectious route based on the
procedures and work practices being
performed the consequences of disease.

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Hazard

 Potential source of harm or adverse health effect

 Hazard associated with lab are viruses , bacteria
or anything which we study in lab and the thing
is harmful for workers

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Risk assessment
 A risk assessment should identify potential
hazards and determine controls that can be
implemented to eliminate or reduce any
risks to employees, operations, and/or
property.

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Need of Risk Assessment

 A newrisk assessment should be completed and

documented each time a new potentially
hazardous experiment is carried out.

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CONT…

 After control measures are implemented,

standard operating procedures and training
should follow to ensure employees are aware of
the correct steps to take and can reference such
material as needed.

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Reduce the risk to

 The staff
 The environment
 The public
 The facility

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Who should do the risk assessment

 Lab supervisor is the main responsible person .

 Every laboratory personnel involved in the
procedure at least review the risk assessment .
 The risk assessment will be reviewed and
discussed with a biosafety officer and
institutional biosafety committee

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Goal of risk assessment

 Reduce , to an acceptable level ,the risk of

worker exposure or environmental
contamination.
 However risk is never zero.

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Consideration for risk assessment

 Pathogen(agent)
 procedure(protocol)
 Protectiveequipment
 Place(Lab facility)
 Personnel(Host)

The Five P’s of risk assessment.

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Steps
 Identify General Hazards
 Evaluate risk
 Risk group acceptable or not
 Action Plan - implement control measures and
create standard operating procedures (SOPs) and
training
 Review adequacy of plan
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Identify Hazards

 Biological organisms
 Toxins
 Include unknown organisms that may be present
in environmental or clinical samples

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Evaluate the risk

 Risk matrix
 Toolthat is used during risk assessment
 Which risk associate with agent
 How much agent spread and we can check what
is the severity of agents.

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CONT…

 Table 1: Use this table to score your hazard or activity’s risk while performing a
risk assessment. Use ‘L’ for low, ‘M’ for medium, ‘H’ for high when describing
your risk in your Risk Assessment Tool.

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CONT…

 Risk evaluation: Agent basis

 What is known about agent?
 Risk group, case fatality rate, incubation period
 Route of transmission , zoonotic? Vector-borne?
 Transmissibility and virulence

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CONT…

 Consider volume and concentration

 Is post-exposure treatment , vaccine or
prophylaxis available ?
 Potential outcomes of exposure
 Natural route of infection/other routes of
infection (parenteral, airborne, ingestion )

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Risk group acceptable or not

 After determine risk decide a risk is acceptable

or not
 Ifyes than proceed with work and monitor the
controls
 If
no than first option is revise the project
 And second option is to implement control
measures and review adequacy of plan
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Action Plan - implement control measures and
create (SOPs) and training

 Once a control measure is identified, the

researcher should take steps in implementing the
control measure into their process prior to
beginning the work.
 A brief standard operating procedure (SOP)
should be completed to document what the
correct procedures are for the process, equipment
or substance at hand.
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CONT…

 Employees should be able to readily review the

SOP.
 Employeesshould also be trained, with training
documented, on the correct procedures.

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Elimination and substitution

 Avoid procedures that generate aerosols

 Use sharp precautions

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Engineering controls

 Select and use the proper primary barriers

 Biological
safety cabinets
 Attend BSC training
 Chemical fume hood

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CONT…

 Safety cups in centrifuges

 Sharps containers
 Safety shower
 Eyewash station

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WORK PRACTICES

 Require more hands on supervision and

enforcement
 No eating or drinking in the lab
 No smoking
 No handling contact lenses
 Dress code
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CONT…

 lab coat hygiene and policies

 No cosmetics
 No hand held electronics for entertainment
 Jewelry considerations

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PPE

 PPE is your last after all other steps have been

taken
 Propertechnique for donning and doffing PPE as
important as having the correct PPE

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CONT…

 Selection of appropriate PPE-

Balance between safety and comfort
• lab coat vs soild front gown
• N-95 mask vs surgical mask
• Double gloves
➢ Attend training on PPE
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Review adequacy plan

 Where there any deviations from the procedure?

 Where there any incidents?
 Is the BSL appropriate?
 Any new information about the agent or the
procedure?
 Feedback from laboratory personnel
 Possible improvements to the procedure 29
RA is a continuous process

 Review of the process is often required by

accrediting agency.
 Thedemand of Risk assessment is always
needed in lab

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