CSET. Name: Ecireuia, Mikay\a Ruth 1. Year and Section: MUS 3-3 Instructor: Mr. Daceld & MQ‘GmM ViNGHYENG Date submitted: Instructions for Output 1. Draw and label the different structures specified for each species. 2. Present your output (pictures and drawings) in a well-organized slide presentation, Entamoeba histolytica Structures to identify: + Cell wall + Cytoplasm * Nuclei + Cigar-shaped chromatoidal body onry matoida) vody + Glycogen mass in cytoplasm Cystic stage, under OIO magnification Entamoeba coli Structures to identify: * Cell wall * Cytoplasm * Nuclei * Splintered end chromatoidal barr * Glycogen mass Cystic stage, under OIO magnification ome2rene 2sEndolimax nana uy Wl ol Structures to identify: * Cell wall * Cytoplasm * Nuclei * Inclusion bodies Cystic stage, under O10 magnification Todamoeba butschlii Structures to identify: * Cell wall * Cytoplasm + Nucleus + Glycogen mass giywagen mass Cystic stage, under O10 magnification a 26 Laboratory Manual in Diagnostic Medical Parasitology with Molecular TechniquesRubric for evaluating student output: 4 = The student demonstrated complete understanding of the activity and complied with the requirements. 3 = The student demonstrated substantial understanding of the activity and complied with the requirements. 2= The student demonstrated limited understanding of the activity and complied with the requirements. 1 = The student demonstrated no understanding of the activity and/or did not comply with the requirements. x “ItemsforEvaluation —SSs—=«wd A 2] 3 4] 1. The microscopic examination was well-drawn and represented as visualized, 2._ The structures were identified and labeled properly. 3. The output (pictures and drawings with appropriate labels) was presented in a well-organized slide presentation and prepared on time. Total score Percent equivalent Evaluator: Signature over printed name Date Evaluator’s qualitative comments:aol eC RL Name: P¢tredid, MikGyia RVih 1. _—-Year and Section: Mi 4-2 | Instructor: Mv. DOWAG 4 MA‘dm Villanuend Date submitted: Instructions for Output 1. Draw and label the different structures specified for each species. 2. Present your output (pictures and drawings) in a well-organized slide presentation. Entamoeba histolytica Structures to identify: * Cytoplasm + Nuclei + Ingested RBC (diagnostic) + Pseudopodia Trophic stage, under OIO magnification Entamoeba coli Structures to identify: * Cytoplasm (dirty looking) + Nuclei + Ingested bacteria (diagnostic) * Short blunt pseudopodia creme 20 Trophic stage, under OIO magnificationEndolimax nana Structures to identify: Cytoplasm Nuclei * Inclusion bodies + Endoplasm with vacuoles * Short and blunt pseudopodia Trophic stage, under OIO magnification Todamoeba butschlii Structures to identify: + Endoplasm with food vacuoles + Nucleus * Glycogen mass * Broad and rounded pseudopodia Trophic stage, under OIO magnification aa 30 Laboratory Manual in Diagnostic Medical Parasitology with Molecular Techniques.Entamoeba gingivalis Structures to identify: * Finely granular cytoplasm * Two nuclei * Blunt, or short, or slender pseudopodia Trophic stage, under O10 magnification coapterzProfoaa EHRubric for evaluating student output: 4 = The student demonstrated complete understanding of the activity and complied with the requirements. 3= The student demonstrated substantial understanding of the activity and complied with the requirements. 2= The student demonstrated limited understanding of the activity and complied with the requirements. 1= The student demonstrated no understanding of the activity and/or did not comply with the requirements. Items for Evaluation. 1, The microscopic examination was well-drawn and represented as visualized. 2. ‘The structures were identified and labeled properly. 3. The output (pictures and drawings with appropriate labels) was presented in a well-organized slide presentation and prepared on time. Total score Percent equivalent Evaluator: Signature over printed name Date Evaluator’s qualitative comments: ae 32 Laboratory Manual in Diagnostic Medical Parasitology with Molecular TechniquesName: potreMa, Mikayla RUihT. __- Year and Section: Mi 9-2 Instructor: MY. DOWAA o M0'G'N Vian end Date submitted: Instructions for Output 1, Draw and label the different structures specified for each species. 2. Present your output (pictures and drawings) in a well-organized slide presentation. Giardia lamblia Structures to identify: + Nuclei with karyosome + Sucking discs + Parabasal body + Flagella * Axostyle * Axoneme Cystic stage, under O10 magnification Giardia lamblia Structures to identify: + Nuclei * Finely granular cytoplasm * Cyst wall + Axoneme Trophozoite stage, under OIO magnification Chuplr Pree 38 aChilomastix mesnili od- LiKe. Meorwoeranee Structures to identify: * Knob-like protuberance © Cyst wall * Nucleus Cystic stage, under OIO magnification Chilomastix mesnili Structures to iden + Spiral groove + Nucleus + Flagella * Achromatic granules * Cytostome Trophozoite stage, under O1O magnification ee 36 Laboratory Manual in Diagnostic Medical Parasitology with Molecular TechniquesTrichomonas vaginalis Flagena r< i Structures to identify: * Undulating membrane * Nucleus * Costa * Axostyle * Flagella Trophozoite stage, under O1O magnification Trichomonas tenax (of Flog Structures to identify: undulating * Flagella membran? 7) me * Undulating membrane * Costa + Axostyle * Densely granular cytoplasm Trophozoite stage, under OIO magnification ‘Chapter 2: Protozoa 37 ysTrichomonas hominis Structures to identify: + Undulating membrane * Nucleus * Costa + Axostyle + Flagella Trophozoite stage, under O10 magnification a 38 Laboratory Manual in Diagnostic Medical Parasitology with Molecular TechniquesRubric for evaluating student output: 4 = The student demonstrated complete understanding of the activity and complied with the requirements. 3=The student demonstrated substantial understanding of the activity and complied with the requirements. 2= The student demonstrated limited understanding of the activity and complied with the requirements. = The student demonstrated no understanding of the activity and/or did not comply with the requirements. Trems for Evaluation 1. The microscopic examination was well-drawn and represented as visualized. 2._ The structures were identified and labeled properly. 3. The output (pictures and drawings with appropriate labels) was presented in a well-organized slide presentation and prepared on time. Total score Percent equivalent Evaluator: Signature over printed name Date Evaluator’s qualitative comments: Chape 2: rte >Name: ecrre1O, MIKOMIORUIN 1. Year and Section: Mus 2-2 Instructor: Mt. DO210_ ¢ W10'AmM VillanueNG Date submitted: Instructions for Output 1. Draw and label the different structures specified for each species. 2. Present your output (pictures and drawings) in a well-organized slide presentation. Balantidium coli Structures to identify: Cell wall * Cytoplasm + Nudei + Cigar-shape chromatoidal body * Glycogen mass in cytoplasm Cystic stage, under O10 magnification Balantidium coli ji Structures to identify: . OSM comirauiie + Cilia 2 yf VaWole Cytoplasm Nuclei Cytostome Macronucleus Micronucleus Contractile vacuole Trophozoite stage, under OIO magnification ‘ Chips rrinaon’ a8 me |Rubric for evaluating student output: 4= The student demonstrated complete understanding of the activity and complied with the requirements. 3= The student demonstrated substantial understanding of the activity and complied with the requirements. 2= The student demonstrated limited understanding of the activity and complied with the requirements. 1= The student demonstrated no understanding of the activity and/or did not comply with the requirements, 1. The microscopic examination was well-drawn and represented as visualized. 2._ The structures were identified and labeled pro) 3. The output (pictures and drawings with appropriate labels) was presented in a well-organized slide presentation and prepared on time. Total score Percent equivalent Evaluator: Signature over printed name Date Evaluator’s qualitative comments: Bx 44 Laboratory Manual in Diagnostic Medical Parasitology with Molecular TechniquesName: ECTre{O, MIKQUIG RV 4 Year and Section: MLS 9-9 Instructor: Mt. DOCAAG & MG'dmM ViNianvena Date submitted: ___ Instructions for Output 1. Draw and label the different structures specified for each species. 2. Present your output (pictures and drawings) in a well-organized slide presentation. Cystoisospora belli ays te Structures to identify: * Neck-like elongated ovoid end * Two-layered cyst wall * Sporoblast + Banana-shaped sporozoites Oocyst, under O10 magnification Cryptosporidium spp. spherical a) Structures to identify: + Red spherical structures * Oocyst, which includes 4 sporozoites Oocyst, under OIO magnificationCyclospora spp. Structures to identify: * Red spherical structures * Oocyst, which includes 4 sporozoites Oocyst, under OIO magnification Toxoplasma gondii Structures to identify: UpTrplasen] * Cytoplasm with reddish tint nuuers * Nucleus (round and ovoid) * Conoid body 3) Trophozoite stage, under OIO magnification axe 48 Laboratory Manual in Diagnostic Medical Parasitology with Molecular TechniquesRubric for evaluating student output: 4 = The student demonstrated complete understanding of the activity and complied with the requirements. 3 = The student demonstrated substantial understanding of the activity and complied with the requirements. 2= The student demonstrated limited understanding of the activity and complied with the requirements. 1= The student demonstrated no understanding of the activity and/or did not comply with the requirements. ~The microscopic examination was welldrawn and represented as visualized. 2._The structures were identified and labeled e The output (pictures and drawings with appropriate Tabels) was presented in a well-organized slide presentation and prepared on time. Total score Evaluator: Signature over printed name Date Evaluator’s qualitative comments: chaprzsPrteroe 49 ¥Name: (tYeio, MIKAYIG RINT ______ Year and Section: MUS 4-2. Instructor: Mir. DAGRAG 9 Ma‘am VilanwevQ Date submitted: Instructions for Output 1. Draw and label the different structures specified for each species. 2. Present your output (pictures and drawings) in a well-organized slide presentation, Plasmodium vivax Structures to identify: * Normal RBC * Presence of red chromatin dot * Bluish ring of cytoplasm Young trophozoite, under HPO magnification Plasmodium vivax Structures to identify: * Erythrocytic schizont containing merozoites * Schuffner’s dots an mers doe Mature schizont, under HPO magnification Couch Prison =Plasmodium vivax Structures to identify: * Violet-colored chromatin * Amoeboid cytoplasm * Pigmentation Microgametocyte, under HPO magnification Plasmodium vivax cytptaarn Structures to identify: * Compact chromatin mass © Cytoplasm * Pigmentation Macrogametocyte, under HPO magnification aul 52 Laboratory Manual in Diagnostic Medical Parasitology with Molecular TechniquesPlasmodium malariae ugroplacmicring rymatin dot Young trophozoite, under HPO magnification Plasmodium malariae Mature schizont, under HPO magnification Structures to identify: * Chromatin dot * Deep blue cytoplasm Structures to identify: + Erythrocytic schizont containing merozoites appearing in rosette form * Ziemman’s dots Chapter 2: Protozoa >Plasmodium malariae Structures to identify: * Reddish chromatin * Pale blue-colored cytoplasm * Pigmentation * Ziemman’s dots Microgametocyte, under HPO magnification Plasmodium malariae Structures to identify: * Compact chromatin mass * Deep blue cytoplasm + Pigmentation Macrogametocyte, under HPO magnification oo 54 Laboratory Manual in Diagnostic Medical Parasitology with Molecular TechniquesPlasmodium falciparum ring form —— mourers e ¥ Structures to identify: aos EO © Ring forms : * Blue-colored cytoplasm AgtOplaLtOlc + Maurer’s dots ring Young trophozoite, under HPO magnification Plasmodium falciparum & A Structures to identify: : * Seen in heavy infections Schizont, under HPO magnification Chapter 2: Protozoa > |Plasmodium falciparum Structures to identify: * Crescent-shaped forms * Pinkish blue-colored cytoplasm + Reddish chromatin granules Microgametocyte, under HPO magnification Plasmodium falciparum Structures to identify: * Crescent forms * Reddish chromatin(compact mass) + Large pigment granules Macrogametocyte, under HPO magnification = 56 Laboratory Manual in Diagnostic Medical Parasitology with Molecular Techniques:Plasmodium ovale Structures to identify: * Oval shape * James’ dots Young trophozoite, under HPO magnification Plasmodium ovale Structures to identify: * Seen in heavy infections Schizont, under HPO magnification Chapter 2: Protozoa >Plasmodium ovale Structures to identify: * Oval shape * James’ dots James'dort Microgametocyte, under HPO magnification Plasmodium ovale ova\shape —_) Structures to identify: * Oval shape * James’ dots Macrogametocyte, under HPO magnification aad 5B Laboratory Manual In Diagnostic Medical Parasitology with Molecular Techniques