*Diccuss riety the tf. using handwritten output! 1 Bilirubin Jests and their Principtes + © Malloy - Welyn Procedure, Bilirobin Pigments in sem or plasma are racked with diaz reagent. TRE diaze ted ewlfanilic acid reacts at the central Methytene carbon of bilirubin SPRUE tue moleste forming 2 molewles of azobitivuvin. This memed is typically Ferformed ay pH 1.2 whert the azovilinbin produad ic red> porpie in color with 2 moximal aeserption of SlOum. whe most commonly used accelerarter Tole” witixe vmonjugated wiliwbin © memanol, although ofner clemicals have beer? veea. © Juudrassik - Grof Metted (Total and Conjugated Bilirubin) Biiwvin pigments in germ or plasma art reacted with a diazo reagent Ceollamitic, acid im wysroemione ccid and cediam nibite>, resulting in tne prediction of awe porple Product azobillwbin. we predoct azebilinbin may be mecicued epeere prote merically: Me individual fractions of bilinbin art determined by Taking 2 aliquots of sample ond reachng one aliquot with the dlazo veagent only and tre other aliquet with Me ditzo veagent and am accelerettor (caffeine - Penteate). tne addition of caffeine- benzoate will solubilize the water insoluble. frouton of wiliivbin ana will yield @ otal biliwbin valet Cail fractions. “the reaction witout fre accelerator will yield conjygatel biliiwbin ony. After o Shere penod of mE, Ime reaction of Ine olllqvots with Me dioee veagent is jerminatea tay THE addition fF ascorbic acd + tHe ascorbic ocd destroys are FUSS digto veagent. the soln FE Men altalinred Sing ow a\valine Jariare SovMEN, whim chifts The alesereance qolemtino, Katrice Jade E. ‘spectrum ot the ozo- Enzymes Clinical Chemictry 2. anal i Foncti Pree ont Mr. Eugene Doyagvylitvein to a more intense We color IMat iS ies CUbjert fo Mberfering gub- wramas iW ME SoMPIE. The final Wwe prodect is meacuied af GOO mm, with dhe amensity of color predvad dittcny proportoval to wiliwbih coneentration Indittor Cononjegatedd) itiwbin may we calcsletted vy Sebtrachag ime conse Lilinbin concentration frm tne total biliwbin concentration. « Nan den Bergh 1s a Chemical reaction used 49 MHEASWE leltimbin Feel in wiesd- Mort épectHcally , i} acrermnes TE ammouwt of conjugated biliMbin ih tre bleed. ThE reaction produces azobitiwbin.L Aspartate aminoteansttra ce (net 2 Manne Francaminase cnt) NTE colaryzes the consersion of aspartal autamate me AST treyme was formerly Frown ag comm gWiamae oalate fromeawinat (S601) ond iS present in all Heeves exept bone, wit Wignest tevels im liver ond skeletal mugete. carayytes the ‘ran Of an amino group from alanine 7 aipna = Keregwtarate ge yrrd ghiomate and pywate as 4 port of amine oud 2 alctincr Koon Tigh lets of AST eymes, on ja no, Sake. be a cign of aghapiosm (e-asd] Kt acy in ena or Plane, ine. | ohemcteots, or hepannc , crrnesisy wuion i derived from heart and Feachom, Ack J Other hiwer aiccarses, RRC, and otter cataryzec tre | High AST emis im mitronondlia T reccning trons | may alse be a a. Acid Phosphatase] cree’ 5. yy aMlamytiraKS forae (GT) ‘2. Belong > the amup of eneymee that catalyeee he hyaroiysic of vanoy Phoc phomonoesters at an alkaline: ph. Mic alco non-specitic. enzyme capavie of reacting wit many diffvent Gibsrates. = Siwilor 49 ALE wa the major aifferenee ithe pH of me action. Ts i on enyme arot fetes attacned prosphey) QPS from other mcteales avving digestion. = tis omagme Ie involved ine tons fee of y-gitamyl wecidie from g-gia] ragh peptides 40 ani c0ds , ¥,0, aed ofver| matt peptides. NP ensle a6 @ nore bor of \ooneymes not can ve devcored by elecrropnorecis Wing cellulose acaraye membrane Pow onedic side, thee are ALP 4, wepatic: (AUP 2), bone (apa), plaantal CALE, intestines (MLPS) and Igo bound ALP CALPGY, “the isoenzymes con be fractionated by dectropworesis inte © bards. frostahe Ne Conds 2-4) for Pestone disease. Band $ tartrate vesistant AR CTRACEY. cansict of 2 ieferms, bands Sq and band 6b. Appear as nam, wight flvorescent bands. Gat has N+ isoenzymes. that can be geen in iver, Kidney, pancreas, intestinal extic , ond Prociate gbnd. Ws coll ve deter wined by generat MP and an ALP isoonzymne est. A generat ALE test i ueeally imetsted i a bioed tert Frown] aS a camprenensi melavolic. panel CeMP) ona a tier acid pl Maction, buf whe addition of olkati cheps the action and trans forme Me produck Wire Steomogens., which con be measwed. ‘spechrophotomichicaly. “We mest widely accepied cvostrane for we iw oer iy aMamy) - p witro anitide «the y-gba wigl etsidve 16 sramsterred +e glyeyl sore, veueaving prritroanitine . “Elevation of ALP are of mosh diognactic. alge wiicemer ia Tre evatiation of epatebitiany ond disorders. yone aided im the uccion & pias tate carcinoma, pormeulorty metas Aasic carcinoma °F Te prostate.