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Improving palm kernel cake nutrition using enzymatic
hydrolysis optimized by Taguchi method Access options

Witida Sathitkowitchai, Sunee Nitisinprasert & Suttipun Keawsompong  

3 Biotech  8, Article number: 407 (2018) Cite this article Buy article PDF

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Enzymatic hydrolysis of palm kernel cake to improve the quality of substrates with multi-
response criteria based on the Taguchi orthogonal array. Nine experimental runs were
performed based on an L9 orthogonal array. Percent substrate, incubation time, and enzyme
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units were optimized considering multiple performance characteristics. Analysis of variance

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was also applied to identify the most significant factors. Results determined percent substrate
as the most important factor for enzymatic hydrolysis followed by incubation time and enzyme Learn more about Institutional subscriptions
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experimental and model results. The experimental result (protein release) at optimal
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Acknowledgements
This research was financially supported by a Research and Researchers for Industries (RRI)
Ph.D. program (PHD56I0043), The Thailand Research Fund (TRF), Betagro Science Centre
Co., Ltd., and Betagro Public Co. Ltd.

Author information

Authors and Affiliations

Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University,
Bangkok, 10900, Thailand
Witida Sathitkowitchai, Sunee Nitisinprasert & Suttipun Keawsompong

Center for Advanced Studies for Agriculture and Food, Kasetsart University
Institute for Advanced Studies, Kasetsart University (CASAF, NRU-KU),
Bangkok, 10900, Thailand
Sunee Nitisinprasert & Suttipun Keawsompong

Corresponding author
Correspondence to Suttipun Keawsompong.

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Conflict of interest
The authors declare that they have no conflict of interest in the publication.

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Cite this article

Sathitkowitchai, W., Nitisinprasert, S. & Keawsompong, S. Improving palm kernel cake
nutrition using enzymatic hydrolysis optimized by Taguchi method. 3 Biotech 8, 407 (2018).
https://doi.org/10.1007/s13205-018-1433-6

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Received Accepted Published

09 March 2018 10 September 2018 14 September 2018

DOI
https://doi.org/10.1007/s13205-018-1433-6

Keywords
Palm kernel cake L9 orthogonal array Enzymatic hydrolysis

Taguchi method

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