Easy slide

differentiation.
~capillaries~
TEM/SEM

Picture 1

*
 On picture 1: we see the continuous capillary.
Red line and the ake indicate the tight junction
between the endothelial cells of capillary and
inside this capillary we see the RBC. In the red
circle, there is a pericyte.

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Pictures same as the Picture 1.
 Picture shows the
continuous capillary.
Structures of it are:

* E - endothelial cells.

* F - collagen brils.

* MF - marginal folds.

* BM - basement
membrane.

* P - pericyte.

* BMp - Basement
membrane of the
pericyte.
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Picture 2
Picture 2, zoomed in.
 Picture 2 shows the fenestrated capillary,
zoomed in picture shows more detailed view
of fenestrations. Lumen of the capillary is
lled almost fully with a monocyte.
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Pictures similar as picture 2.

* F - Fenestrations

* EC - Endothelial
cytoplasm
* BM - Basement
membrane (*which is
intact!)

* EC - Endothelial
Cytoplasm.

* D - diaphragm (I have no
idea what this is)
 On these SEM
pictures we see
pericytes wrapped
around the capillaries.
Capillary, longitudinal section showing:

BM - basement membrane.

J - Junctions of endothelial cells?

C-cytoplasm of endothelial cells.

Light microscopy
images.
Picture A
 Picture A shows:

Sinusoidal capillary, therefore it could be located in the liver, spleen, or

placenta.
Picture B, which shows by
the arrows the continuous
capillary, this type of
capillaries are in most
places in our body, this
one is in mesentery.
1. Write exact
blood vessel and
why do you think
so?
2. Where could this
type of capillary
be located?
3. What are
indicated by the
numbers 1; 2; 3;
4.
4. What is the
function of the
structure 4?
Answers to the quiz questions:

1. This blood vessel is a continuous capillary. I think so because there are

no fenestrations in the endothelium visible.

2. Continuous capillary is predominant in most organs and it could be

located in the muscles, connective tissue, nervous tissue and etc.

3. Structures:

* N1 - junction complexes.

* N2 - transcytotic vesicles (but she might mean basal lamina, ras gaugeb
magas).

* N3 - same as the number one ideashi but you can say adherent junctions.

* N4 - I think it is the endothelial cell. (ver davinaxe fotoshi raze midioda)

4. Functions of the endothelial cells are various:

* It is nonthrombigenic surface.

* Regulates local vascular tone and blood ow.

* It has several functions in in ammation and immune responses.

* Endothelial cells secrete various growth factors.

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 Summary of capillaries:
* There are three general types of capillaries: Continuous capillaries,
Fenestrated capillaries, and sinusoidal capillaries.

* Continuous capillaries - Their endothelial cells as well as basement

membrane, are continuous and they have tight, visible junctions in
TEM.

* Fenestrated capillaries - Their endothelial cells have fenestrations

(gaps) but their Basement membrane is intact. They’re seen in the
secretory or resorptive organs like: Kidney, intestine and endocrine
glands.

* Sinusoidal capillaries - They are very discontinuous and are mainly

seen in Liver, bone marrow, placenta and spleen, where they allow
passage of macromolecules.
* Functions of Pericytes -
* pericytes are frequently seen on the capillaries and pericytes are able
to contract, so essentially pericytes are helper cells which help in
vasoconstriction.

* Pericytes in CNS maintain the endothelial Blood-brain barrier

* After injuries, pericytes can proliferate and di erentiate into smooth

muscle cells and they can produce other cells too.

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Picture C
 Picture C shows just 1 endothelial cell, where we
see:

* P - pinocytotic vesicles

* W - weibel palade bodies.

- Function of weibel palade bodies is that they

act as post-synthesis storage. Weibel palade
bodies contain von willebrand factor, and other
proteins that contribute to in ammation,
angiogenesis and tissue repair.
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