KEMENTERIAN PERINDUSTRIAN REPUBLIK INDONESIA BADAN PENGEMBANGAN SUMBER DAYA MANUSIA INSUSTRI ‘SEKOLAH MENENGAH KEJURUAN - SMAK BOGOR Kode Ook 1S. XI- OF LKS ANALISIS _ al INSTRUMEN baal ae _ KELAS XI TARUN [Toten 20 Agustus 2023 2022/2023, ~ aamnan tens "RNALISIS KADAR THIAMINE DALAM TABLET VITAMIN B1 SECARA HPLC. Nama Kelas NS Tanggal JUDUL PENETAPAN: ANALISIS KADAR THIAMINE DALAM TABLET VITAMIN 6! SECARA HPLC 1. Prinsip Analisis (20): Kedar thanine datum, EeHet vikewin 81 dapat ditentlean mang genalen cnitemmen HPL. HoLe [High Papamence Ligne Chromsbography adalah fink, eretede peniiarin mulelay on toemanfaathian et ent sebagal fre getalrye dan dihant dingan eleanan dae” pomp sels dapat rempercezt lay alic schingga didapeslean dain Yoong dideteks; oleh deteletor beryps kdcmeregrams Tngteunen HPLC ine dapat dranatean unkle @retsrs sechy Sena yang frdek stabi pada subv tenggr den Senyawn syarg fede ouch nang Sete trngleat Vestablennye rendah - Pade peretapen ini dgrcleer by perfor !ebhand 255: 45 Sebagar pate gorge don hei GraQtinge alee: pod, Perjerg golomharg 254 0m dengan Abteliter O40 . Aist daa Bahan 5 G Ale BP chen Keo Agi 1. Paper wnat ome {Mig POy fable 2. Nena dghl ts. Ship & helen 2. Pyubides 3. Leh Uae Som 1a, Perstacs beh 3. Thain. Het As Pde gale ein 15, Cenry hucle 4 Tablet Vitenen 1 S. Owe “1G Paty goles Bont S Menrane filer PATE ous so © Corerg, (TE Pda goles teoml 6. Membocan filler PYOF 012 um 1. Pahd. senpct 1B. feel pelank 7 Baer popes maths! 9. Persad (9. Vrad Spot as ea wn fetes De. Syemge 9, Hotes sermy Uhstmcan a6 ay Le ole SodepML. Persiapan Sampel (20): a Kode sampet Ty. Penerimaa Identitikast sampel 203/vBl /LA\3 /or{202, 22h Qe 20 Sifat fisik sampet Wujud padaticaitlezs : fubjm blot Warna Bau Bagan Kerja 2b thie -Pembuatan Pelarut Butfer Fosfat 0,04 M see ys omy” Eble KH,P0y Diseciog, > trenten fille —> Dirnsilen ee PETE os pam PLinere, Dinsuhleen le dalann alan equate hkl pdarvt , tebe cher Leyak -Pembuaten Deret Standar Thiamin (0-50 ppm) Set Drprene phen Dawrig be som there tent, (oi dlenegon ae To od gen Other deo Cheer three. tee Grngsoreteg — * 2. kg ria, PASO EN on Theamin. WO cay Drasiaon ba WY Sent reed Gp) 9 em ee F batter per ears) 2 as Dane Sted —_£ 5 Pringetetenn dren si we he HO yo end fee - oe on filer if opm -Persiapan Sampel =— tae, ——> — aise tte? AP os, Vitam af Oibnden 2 tai” Pmbey $019 ye ta Wine Warr 81 Ssapd wh ot hes thepe etdytint) Okaale thew Quen sone S* den dterphen kee 3 oy Ky — é Prgjeckecn nae! — fa tte“Parameter HPLC Par came, vee Fece genie Bagter tafe: ethan Fla Rete 1 mt low Step Tine A ovest Lajatron Valine 20h Derek ter DAO 254 0m She lelen, 4% IV. Data Hasil Pengamatan (18): 4. Data Penimbangan [ Tablet Vern 1099 Ector 1 Tablet Vitamin BY 2078 > e266. 2, 006 CATER 2. Data Pengukuran Konsentiast | way can omy | Waktu retenst Area Keterang: ° 2AIB baat Fo eT ET cry 2,393 lions + 30 23 2234391 33 40 aa aap2404 32 Go| 3389 Tsu z=) t %_| Sampel obat sintesis | Simla Z ae (Untuk Deret ad Standar) . ct [Dupe 7 loa a oe SMK-SMat 3Vs Pengolahan Data Has pengukuran 25): Grafik Standar Thiamine n¢ Konsentrasi| rea-Blanko 3500000 y= 70895-71417 {ppm) 3000000 R= 09977, 9 jg 2500000 10| 604579} 3 rnc ° 20] 1350075] & secon . 302079384) scocc09 aol 2837ai7| F \ 50} 3405633] o Simplo 904093} o 10 20 30, 40, 50, 60 Duplo 922366] Konsentrasi (ppm) ~ _Perhitungan Konsentasi Saipel dengan Derot Standar Rt 69977 o Som = Cpenk rer rmrsen) fa ee * & ang, i Ba reds Sep Csonsz cmt) ee POOF SE . O16 = + (grese6 -C- 1107, (922366 -C-21020)) vse Toaes, 7 | = 140718 ppm | Perhtungan Kader hm stam (dan (grab |B ieador Thane = ppm i vlna by gine] 1 east Thientn (mg hig) > LThoavin ebt cantare tablet *y ood ‘ 1 © Smile = Gael a, Omi + Ween cure te Gast 201,709 | eae 31Bo_ glia | | * 684] © das = > Duh = 692% K 201,29 09 F tee be os eo (so ita ot = oe] Thame] Zz 0> F Thieow (lla) = 15,g0 413,96 —<— > Otay ag Gest = 151¢0 og liable 5 leg.“ \ieae [No + 82657 pele Vehoterimncn 5 tog Uhablat £105 0-9 a rdis Myllsblet ~ Locs oy tablesVI. Pembahasan (10): Pode peretapen Kedar thane. dadarr Geek vitamin OL Seen HO Treberapa perentes?, your but per fosfst yoy aguralean sebagar potent vahle mempetshenlcen Shrletur dent thramine agar tetap srenjedt Ton dan tadsk berbah Penfaat goramny Dika thramine leibentele toes gong mala hal fesebt dapat Aeon hn blaan vetbatnn pada elem dan apar” merye lanblean penbsencn alten Jeldetor tidak sescat. Pads penatapan toi pemap MLC diigunalean Teversed phase dimana for gouk ~9 digyraleon becsipat pee, gee digenclean bup fer fosfrt em taathrarel dargon perbandingan ss24s , Lalu vabk fare damage besipat ten-pllar pyothe digestion Cl Paraneler pada HAC perks disesatkian dengan saapel , hal Py behpan 2928 hess] deayatlean werdekcali eile? yorg sebenamys LThtenin’ promi sifar gory pale, Sehogga fase gore gary Argunaleon hoersifst olor dan fare drameys herifar nen-polw: Fou rate diab sebesor Lob [oan ; step tre dere denyon vole injelesr seboryale 20 BL «Thiamine menspalen Senyeors oven re’ diahe .untvk Mencegsh fusekaya sthtw Enrarsine keomefor 9? olapet tory abserbs smear UV, maker den Thy Aigunakion delekter DAD (Prede Prony Delecter) dengan Png gelewbang 250m. Hast penelope didlegstleon Kedar Uhiamora sdoesee 13:88 gltablet. dorgon oiled ADD sebesae Wis chan ashe reseveey Sebesor 92,537; diteona ed berseloot Prenenchi pesyarsten akursar gouty go Nob. 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