Scribd Logo
Upload

Welcome to Scribd!

  • 主要食物过敏致敏机理的研究 13722051

    Uploaded by

    Sabo
    13 views206 pages

    Original Title

    主要食物过敏致敏机理的研究_13722051

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document

    Copyright:

    © All Rights Reserved
    Download as PDF or read online from Scribd
    Flag for inappropriate content
    13 views206 pages

    主要食物过敏致敏机理的研究 13722051

    Uploaded by

    Sabo

    Copyright:

    © All Rights Reserved
    Download as PDF or read online from Scribd
    Flag for inappropriate content
    of 206
    ABB HE F ® P ieee ta EYRE SEG IREa Near Allergenicity of the Major Food Allergen DF AKL mie | REESE ERR Dt BK DLERAS OF FE AMER GH SFA ARK 3 ZEA B ( CIP) Sd EE RAL OE A, BC Ae BBLS Tb tH GAL 2015. 3 ISBN 978 ~7 ~5184 -0237 -3 1D 1. De Qi ML Deena - H9E WV. DRSI.1 fF RCA PA FB AE CIP BABE (2014 ) 5% 300980 HEMT ee HERR TT STATE PRI Bits AS DEREK AT HAR ACT =H RS Tet RCE CA AR BEAK 6 > , BAG : 100740 ) il ACH ATH EMA RES A AE UW: 2015 4 3 FY 1 A 1 UC EBL A:787 x 1092 1/16 EK 13 a] Hue RRR S H: ISBN 978-7 -5184-0237-3 fff 40.00 7G HIGIEY HEL i 2010 - 65241695 F&I 65128352 BATH 010 - 85119835 85119793 f& HL 85113293 Fk shttp: //www. chlip. com. en Email ;club@ chlip. com. en ‘OA P15 AGE HE 5 ER HH 130499K1X101ZBW Ok Bi PTL BE — ME FE ASE TVET DAE s HERE 49% A A A, FB aE AEE AS 180 BLA Le AT ARAN AK 90% WL be Bey BE FEF PAL OE fH FEAST th FEE ID ERR NBG 8 KAP RT PCBS EARNER} EI AAS OX HE eH AB TH EE GT TP LE, ELA TE. LE ABR CE ES HEEB EA 7° ST Bt A AIS, BO BSED] SE AAI, , FSR RY a SBR A BH ABH EE HE POMEL BORAT ERR 1 FE TIM, LAA BL Ae TURAN WE RAH, PRB STE SAREE AEE EEE A AERTS BA BCT EA Sh ACA BE A TET OE SE BEY A AEB AR BE Fat EF TCAD SED LE, RT TAC AH BOR EE EIR, ERE ST RT nt BY ESL AE FEL ti REAR, AG SE AT FE, OE RR SE AE EASES AE BEIT ACD FD 9 I HT BIB ABT HH: 5 ES EP EEO AST SAT Bn BPE AUR ARLE, DAP ERLE ML PRL TE EE SBR BRIE Tht FY HEIDE IAS FR Be ESP Ab i ABC ya OS TAT RAREST. & UCP, FHI SERA ETL VLR ae BEE BET SH OF aE 11D PRT TEE A EAI IBEARC AT 3 5 5 DLA AL, Hy TOE ET ah Oy AL AB DE 9 AOL, ee Ek RE FL BH BES ABR HE TATA IA AR IB ET SL ORB A LE FERRIER AN PRT FLARE I ORL ARAL ih BE DB AIR AL ET A TBI LIL ARTCBARCE HS 5 PS ELE BE BY ls BS PE HB ABOU BE, SEL A HL SAE Fy RE ET SASH 92 BOLT PT 9 AB I GUE AT AF FET RBG FE AAV EA FD lt HB SBA A RAG ST Bs 1 SL, een TL Ag PR SSH, PRE, BET BUNA ARR Z Nb AR Se Se DB — Da AS eS AR fF) EG 9 | BA, A CL DESERT OH 10 ZEAE TAT OTR EE OF TL, GA EI EA RAT aE. i BRIE BAER AE RS RE SBR, PE EE RCH DSTA TTT A: RPS TAG Ie TREE AR LS Ae HH SHS, MR TE BT AER Ge ACT A ae Se FS TL CT Bh TE A DDL, RE RR FRATE MAR OA ZN FT. SHED , XU BE ATURE A ET EP HT ACTA, PES MOB RELBAE 2014 12 AFAR Se RRA hs teat Ko teeta R= yURER ~ SUNS ooh AU KO SETA RCAABEMT EG nnn Toe VE SURRALEOTTR SRS TABACUM AE SIA Ara hl SRR ERATE. SRSA IMT esta Tere SFSU NR A HH ETE SIT RA A A ce AA A ESE BA Sie Sain denensneeoeeee: SER ARMAMENT RK ATRIUM A RECTOR - SRG rR DRAIN Ca a Be Ka ATTRA RMCTAMEEL SAU RECKONS REAP OTSE SEH HOGER once nnensee HOR PML SA ALARA NLT SAA — MVTREYAI B — ALR HURDLE LENT SAT ASUS AE RSNA A REE 2 2 CH Seni Hie sme HER mA GRAAL IRL LHL RA ARBOR MEARE ETOH oo SAS IH PLAT SFC 17000 BEA — RE HF SA BOLT TART MW aH HE SUS ERA INH WS MEO STL RBG DM A HT S-H RHUARNBARRR BA LEER —. ROS BRI SREP REGIA A FAARRC RHEE, SBCA —PMRP HERI, AELEERMARTIR BEATER OUT AAT AEA PERCH RUA LY UT Ae LASS PERILS UA IE IEPA, BUEN A ABU ( hypersensitivity )"" BAB Z WAL — ARE FH (19 SE BN A Se AS BL dG — ARR AB Se PREZ 1 Ld AE AAR), BO A AS BS AE LPR AF J TTI PARMA AUER. 51S AE IV AOE DRC Dy BER (allergen) , LAE WAL BUR. AE JMR AARZS , AY ASE SETI ATED AE SA TE) ABT OR Co ACE Hil 26H), HEN BSE 1963 4F, Cell Ail Coombs HMMM PH AAA MEAS SAM HURST SAUL INLAY AT RUA Be AG TEAM SG TA AB} WY”) ( he 1 - 1+), RAS LH (al- ergy) af #952 Ni ( anaphylaxis) Hse 1 UB Ae 7A OS MM, AN SA I. AWM SAALD - BCPA BE: * IEF — ABE BET BE ELAR TH SER FAA — PELE IR , EAS BENE SLIEMA A” (ES BI A IL IN, TAIRA HSER AEE SA REAR A” CE PA BR BAY CE EC A LE FE DE HE PERE SES RL SAE A OE TT | A «SP PD SIS — IMAG A, Be RAE HE , 4 A ML AT SH GB 2 HH BL OR HR EAR AS) ABC RRC A, iS He A YH He , BA HG BE SFA FA ERIE AAS YT SP BEL ABT BEA AS A SA TD LAA 9 SE RBC ALDUUR XT BT BETES LPR BT BE MH A LUCE Be BAC ih IT BR Se Et $9, BU TTP tx oka BZ, Rl-1 BERLHDRER a Tn ERR 1a Tee AAR RAE TE EP SE ER (RRMA) BIL IKE —RAMASY SHV (complement mediated eyto- 18 toxicity CMC) , POAESEHEC HA: (leM BR IgG) $5 REAL A JLFR a, Se eH HE (MRE HE) USSU DRER 5 CP StS 9 RG ES ASB He REP AMAR PA A RA, DS HE HE BT LERVURAGMBHAA Be i a Eee ma SBE ASD TLE ALTER COM) ARDY SO AL MLA EAL EER PELE ERE, (RAE AM) BREE RED RG Ne SPEEA T SOURS MA BS 9 EH _ x I Fe ALI 28 % Gian) setae AYE Ee AHR SA =. tte XL Pe RL AS Ps 0 BI I, BO TE AS BL NE food allergy) , Fete PE MIBEA A Hi, BLUR REZ AE EAE HENLE, SBCA He BE BB 9 FL BR A HE TTS | AZ — FA Bh REAR, HBG Ig EO AOL USL EPA, PLAT SERCH") 90% LAE AY eeebN tt ACE BE, fi TAS FL AEE AL MERA AE 8 ASA BAH Be) SL AL ARR SE BOK SARC, PE i SI APS A SA FG EB PC WAR BR IZIN( adverse reaction to food) J&48 HY Ce CA} MAE sh HS IN FAL S| LAY — AR Bh SIM, Te Be BE BR INNA Be BE BBL , HAE BE AK (food allergy , FA) , Bll Ee 7 8 fat JRF (food hypersensitivity ,FH) ak fe 9975 AS 12 Mi; BY) ANIT SE (food intolerance ,F1) fii Fla # , FEAE RREDL BA AE AY EBA BIL INE 6 =. ROTHER SLE EO EH GL A ZI ST A bY BO AR ALL NB AS) EE HR PENT A LBLIL LAE A VBE AGE RT LEAS TR BE DD PERE 7 AE ASE A BI BED A SBE HE — A VAS i BRR ANS kU ER HEB eH a SARA: PE ABU TEAS RH Rs RY PB Dh BS A AR A EAR AL WERT AE 22 AGP EL TE ALE AT 170 ee A AHP IBURST 5 FY VA SE OP HWA Dy 5 FEAL ea BD OY eI AS PAE ARR BA TE J PEA RBBB TEA TR AYP HS Ae TY RB Be RU HER BE, AER AEA MRT a, BBD Uh EAA A AE USE A Ae BA AR, FER Ba I AB HR EB ZF WR, ALE AE SEs AL HS Ae FR BB AEE) BDO REA ts BD 2 ARE AIA eS bP Be ANE AT ADA Sy See eet Ae 8 i BE AR B9E BN bf Ey Cy BRB I NL FS AG BI HB TL Sy AS TA BB AS HY BH Ai AD DOAN ALP HE I WR I ER BREA HL BR BE, IAS iA HEE ARR EAR He BREE L, ATIB A PE 5 Ae Bz UR BORE 1 1 19 A SEER A IgE BK BRITA AB AEF AE A BEY SERRE IA gE Sic ASE RE EERE A BP (BT HE SEAR) JE AR S/F 2 Ea BE AR BY SR A Te GOH, RE IgE SUE BREAD PH A ES eB 2 SOE TDG A ACHR A AFG dS ES A, FED) PERG BORE IBM ARREAE: } RE PRESIDE AS SE I A RHE” 2 SE ROMER RAR DO. Tg Sp S64 ih WL eset ABN EAR Ug, Sp SE EAS I ILA Ae RE AR AS AN — ET DA A ASI FB A iB eo ABATE aL ACE i ASL Pe BB ES ASD SS DO He FR BE HL ABR EAE ED REAR RE A A) AHAB AE TL BIR IY i BN A st ABI MLA 30% 9 REAL AREY A PB LS ESOL BSE ML, FE BEAR Ey FY AE HE KP RE AS BR PY TEs AB LR Be AML AER LLY oi eB A AHIR MEY S0% , SE BEAR HE Be Week BOT Tm , 28 ow eh FRIMLEY) 80% , LISI PENDS ACHE I ARCH EMRE MPS HE KE 5 ES ABE Fi EERE Aa WA IHG RAL MAE SI 5 PEE AB Dy Ze I SEE, AEST OST RA PEE Oia ER — TA BEART 1 REI A Be eh A BA VIEL ABI INL) 20% BE HA IL Sk ABR NA IPR SE I BI BK HE K, Wwe yam AR PE PITE BREAK AIM FR IE ASL 5 BD PE I RE BBS A I, tH Tot EY 5 HK BHEHE, 1. MRA RSE ATL BL PS BE RSE CAE, BAA Te |e at BHA, BT BERK , A Ph Be it BAI SEA AAP 2. RORE EFA URAL A, WIE BER AT | eH TF — He I, Be HE RNA BURA , TUR Te BR — se ya RS DTEFERLE Lo 3. BRA SUE TAR EEL EDO Pit EEE. SORE — Dr 4 AE Bas, SUP ee PT A RRA 30% ~ 40% 5 AESLAE RL Aa Hy 1B AA EAE BEI, FLT IS IU BG 3K 60% ~ 80% '°? (Fo AST RP TT AS TD ak AE AR TS tL 2 I SLAB ZETA | ABR GE ABEL, TH Ae ACER AB FEAR A TB 4 BENT ARR AR AF] — 9 A) FY Am AS DS A A 9 TRAE PARAS. HAR APSE IY WT ESE ABI 9 SHA ae — A, MUTE PEP LEE LS AT PT BABEL ME FERTILE AE Be AB fe ES HFA JL EAA SUL FREE REAR SEM, BED BE EAE 5 So OR UE EA 5 | st Be MB ALY yt ABR, TL AA FLA DFE aT BE SN AT HF eI AA LI, EAE AE EE QPEL AREA HE MFA TT Be A EE AIS LA He 3 oy LERMUARRAM BHT ‘a es EDR AS RE, BE I TSA AT HE AB AS i — 2 LB SR, ROB — PLB FA 1 EE 2 Hm, CTR APSR ES AH BE TE AY DB Bk IE NE BAI RAB St I EP Ff CH BER D ARV AY BE A AT AS SE TAD I RE HE, HEL RIAL FF AEE LIE. BN, 2B DAE 50% PLAS BALE LL PALL ABC, RR BE BAT fs SS BE A, A AA ELT AE RY SH CA A kT Baldo 2°") 3238, ADAP AY 45-492 al, Bi 40 Fa I FARAH ( Poaceae) SAKAI IK AR EK, NNBE ABE AREAS Ui) PT AAG BE GE INT, ECR BE EATER EY RRR AY TEMES ABW TAIT Ne BE OR AE AY BE EZ — , EA AN KM KADY AREER BE INAS AK 9 PA, SUAS DG BER ATT HL AS Wd AS Td 19 2S SA HE A AY SERED ENT AE BLAIR ABO TR, SOE A SS ft BE FAD AY Ey a AR = ES HH A J TP (0B i ES NFU Ae Aa HAS Eh oT RR BERG EA I Hh ET ht BUPA. —. BURA BP 90% FY i AE ED, KE BOW TK PAE EE, HARES) FEE 10000 ~ 80000 , FLAT SAME AY = ABSA PY CSC — AR BE A, ROA OT ae TS ARETE SE Ae BAM, AAA, IF ERA FS, EAT ES TB RE AED AD Be CAL ORB Bh HE SH DE HE A EK AE A Bet vd AG BEA A — iL HA FP 9 — BB BS PT a ER A PEAT OL Fi FM EAE AR AK, IT EL BSA TE Ct EE A TG NB A ARE EAD OU IP aN — BEA AEST BL 7S RAE A YE se HE A SPARHERE A) AERA PRE TT REALS TA EA A LE ER, RORBL, SOA DTT IgE HAA IE IE EP RK 1. AHERMTAEA HOTEL OUR TD ER HE A) Ete DIOR AP UKG RARE BS, At A TL RESET A BR Aco 1993 4%, Ogawa SF") 55786 Ht AOR AY 3 BE ABCA FS J Glym BOOK , i eB EL ABE EIR IF UTR As BEAT EEE, WET ATT BSE BP = BE a 34 Glym Bd30K,Glym Bd28K #il p-7S (EAD RREA. PALA AY EAN RAD PW DR KG ERE Rt ARR, ATT SBE AEE. 1978 48 Shibasaki 4 ill T 7k ABE AAT Et AE FA, 1987 4F Matsuda “iz JH] HL PC\E LISA, SDS - PAGE BR AUPE PS AG Hi — BP FE LEE EFL BY BR, HE FE BS PRGTL AURA FP Pro Al Cys et HCA Bi A AUR BE BES, I APL ACHR A HE 100°C FE 60min JEVHR FT GRAF 60% BE ABI ILI”). Luh E— A SS AAS AE I FBR} Hg 14000 ,15500 16000 ,26000 33000 Fil 56000 , If He HLA} EE 14000 ~ 16000 fk ABC FL LA — Be A) RE FL EEA) Fg 33000 Be AR A 4 S-E RHNERN RTT SA TITANS” 5 2. inte eA ALTE LTS — APF ER REM 8 - FLRREA ( B -lactoglobulin, BLG) , @ - FLAC @ lactalbumin, aLA) 3 ; 928 SEP A HAS Ds OBE EIS A A PBC. BB HP BE EE BH GR A Legh Sie EN GEA) BSE He Be OE A Pe EAS | RL AM AE PS SE ES TS AL HHS, Masara 25!) ESE AE SAL, WE AS Be WH FB HE A JH (1 3 BE A BG hh AH ( Crassostrea gigas ) it MUR — AH BE Cly Ala, Leu ffi > Pro, Try FAY Ja WLAR HE FA, SEARLES) it 24 86000, Sy Pas SAAC SA BAA WEB) A CFS LAH A HL A FS FB TEFEN BAY A AAR BR FD AG PAS 0) NS AR A BIR, , SIVA HE RS ALE LEAD SSE A HE AB EO, =. POE RH EAE: Dy Be A OK EDD RAL RA BS A AE: YY cb PE HAA FE A 15 EA 7 BI AT Ba a AE EB EYER, Nordlee °°!) BLO Sea, — Ae MEDAL ee Fe AT AP ES RY BE AT AAG ASE ABRIL KAGE Be RT BA FAL ZR BiG LE HRT ZS Be a JSR. A ARARGE FeA EA Be EEA Ee LOE ERE Pa A, Bt FE (PDS BRAT A BER) FE , Be BEA — A ARIE RE ALR EA HE HP Bt ETA AEG AA FT ER LR, LS, 5 Ar BB A Lh EM A, PB BE FA Oe HE Mii SHE, i ABCD 3: Moe Fl 4, Ae (AB le PLB i PIT A SE OK FEE FA BF I SC PAY A LAB J AS ALAR 2K Ue GSR SCA IT VA ABET FE ABA RY HG SE BL sl HT A SB A EH DESY RUE BEB T I AAG aR A ASL ADGA ADL) 0 LS Ze SB) 3: ES PELE — AE AY RRS, VATED TERE HE BU Me ARE BE TA ET a RE TARA A ROMA BAP SAAS PEO ACR FH SEPT BU" AIRSLABAA AR” A 2000 448 , FR BIE Fe Re Ds PBR CE SAB HD HB SE IRAE FRAP IT PETE AE EL Ba PURER SEAMS F 5 BEARS SE A MRL RTA ATE TE BS yD ae BR (Ati gen Determinant, AD) , —“4iJi4}F AA — At MA AAS A] AY UR EE HE, AA EAT ARLE, FA — FA SB Be A, BA Tk SRE NL AS CE HR SATE TERR ERE FEAEFTLE SY FPSB BB aE EBS WBC EH TE th ee BE ASH 5 LERMAN R (AO FR a Sa A Pe Ah SR BT Hy AS EB De (PL 1) 0 GLARE AT PR Ht HT aE AE, BT A AL RE BAB SO BEY 1-2fifas. AREAS T ANZ EATERY T SRL Re ET SCPE SAY B Sma Be SINE EAS sy BSN GL DRT EA 5 GT EA BOT Aa — a 1 Td HE AHL TRE RIESE MRA IB A BAG HDT A EI Be) JL SA 3 IR ERATE BAAN GE FF FI SL — ALA Dy TS RD RR EERE ETB A HRT DSC ee BE RO EE EIA Sh, — LR EER — KH) FH AST A FY SERFS RLS , ey Be EB, GO FR a SE A POE FNS ALAS , PR AR ESET REI. LEBEL RVR BE BY ERIE BON 6 , AEE ADR SE BRIE DH PEEP RHE BO 16. TS EL RB SEE — fy 7] KBR (12 ~ 18 44 A) FERC Ab IEP NAF HE D B PAL — 1 BEAR RHE Dea (A BCD) AURRWCHE DEE RR (EFC) TO Be i Bema IE AE 4 / ret l 2 fim FREARAT PAL 2 RDF) T SOLA ESB AL DD EE AT REET EF FEE Ho ALOE BY PT A RES Te cE ELC Ay RAE HE SGU PEAT HEPC A ee BEC FA, ER AES Hy See SB HEE HAL (immunodominant group) . Hil WIFE DT (452 Bt antigenic valence) S24 RE SHUAGE A AY DEAE BEN BC. A BEDE ER AE AAD FP — MAR RAE SERB, KB IR BOTA BP OEE ES Vik ALN, A LIES AA 18 SRE BE OE Fae TATA 10 Mt IE BE. S-E RHVARNBARER SOT TLR MTT SPT Bb Bh PE A BE AL A EST BER SD ARS BERS (skin prick test, SPT) AIL FT Xt HR $e WII Be Ie [AK F ( double-blinded , placebo -con- trolled food challenges ,DBPCFC ) ; (4b 4% (45 2H Iie FRIAS: ( histamine releasing test HRT) Alig FAN gk —. RAE 1, BARS TAYE AS I A BE DEM ( SPT) FE AC TE FB BE DE DR (GL ABIES A Bf BE PAE A AMG ASSET ALA IgE SCA ENR SZ ES, LoL — ARB BYE He EE A ARLIB ABURS , REC SALBe : AATT y PED GS , t R A A E i RAR 5 1 EH WA ABE SP FE AE, ETT EER. SPT A BE HE 7 SLAB LS BEG SP He A A) BI Hl, A — HE 9, WME ER A GUI aE REL AU A: PP HE) PERT FB AE (BID A BE BR AC HE) BC MESURE AG 498 HE(10mg/mL histamine equivalent prick ,1OHEP) 227%. SPT FAIR (H(A TF ALE CE As BEES EER, RES AEE LO ATRIA US, HRA LIE PHF AE PARE EB EFF IEMs EAR FDL BR A 1 9 250 , SB EU TRIER TERRE SP TT AR ee BEE, BERR ATS SA IPA TE — BL, BERBER REARS HH UREA , ATR , HEB ESA SRA HH LE ABE AR, PAL te SPT ES MAES A POT VGA EA 2. AMORA YR FATE HLSK (Open food challenge, OFC) , #4 FY (Single -blind placebo -controlled food challenge ,SBPCFC) 83014 (DBPCFC) S:5@7kUEFT, 1976 4F , May AGI A SME FP BU Ae aL AACR I, BERIT SHO PIL HC AY BING PE FLAT 29% Bock "Rt 500 ‘LLIN EAT HLT SBS STE BTA Hy A SBR BRE Be, DC EAS AI DS Ee FRET SBR a ME — FANT ER TL ABR OH HE SE HT FE AI I HEA. BU eR THO LEER HE ARS 7 ~ 14d, BAR EEL BO HEAT. PMT 10mg Fre , WA HY BOAT EAR , ERA 15S ~ 60min HEALER, EES 8 ~ 10g, 240 FEREAR EBL, AT HER BCT A, Sy Sb Ee PUFA Sb SBA I ADE Bt tH AD AE OT HE, HR SE Re ATE. BAS DEE ANSP FE FA Me IOAN Neh EST TE: 1. ARO SELMER FCS (HIRT) Set ABR DB Ae SABO RA, | 2 Ae Se rT PEM EK WE PETE SG Ha TE FE 5 HEL LS MR EAT ABE A SAL TB HE HE, DA, 7 SEERVAARAAMEHAR TORE A PEI EBS EAT SHS gE SCAR PEPE SR, DORE SP A BBS) HBATESAR , VFEPR SCOR TT EP 2H EATERS EERE. HES SEE FT A HEI OAR AS ABE, FH LAR AA HSE FY As A REE, 9] I PRL, LHe SUSAR i De AA A LR 2 1gk SEARS Lg 5 Ay Ea ABE Ae OA PE NS PS, PAL Se A EE IGE 4 TERCERA DET PA EB, (1) RAST fi S2HO-A1 EAST HN GSCHe AVA IgE ARIAL BL IgE SUA tl 4 Je, Jo SEL ABI BASH (radio allergo sorbent test, RAST) BLUBITKIN . ZAHER MRC Se AACS BALAN il SME HEAR ( enzyme linked allergosorbent assays,EAST) BSE AML? Ibi FAS er Be AS Bt GRR ME SECE IgE Sh ABA PF SE BRIT RK RESEE Vee HAAS Be EARLS Fo ED A BAS He HE AT TP ER FLARE A a AE A Ha TH PE RE A Ds be BE AS I RB FYE A BU FIP HB RAR Das LAP OT AR HE EAR. RAST AA EAST $0) al SMG A) — tS BEAR A FF CTP EE AIRE RE — SB, A AAT MEL MEAL. RF EAT AT DAF SR BE ABR EU A IgE SEE AS WE PEAK BRR TBE Ta PA TE EH I A OF (2) IEF-PAGE #l SDS-PAGE EE) UE Sc4 GHUEGPFHL FEA HA TK(IEF-PAGE) #1 SDS- PAGE Weak ( RL 061 LPL CEA) SEs ee EDI SP MT AT ASAE AT AAA it I AR 9 SP RE o SDS-PAGE S92 EG A BTPT LAF FILS AT ACRE RIT To TIA LEK A PA WARD TC A a EW FPS Td 29 PAE APF. 4G RAST Ail EAST 2E AVA, Se ASE EDGE AT TS TAPPE AT VASA Le: 0) il Sz BT BR AS TP BY AS Dd oh Sk ABC] BE RRM. BEEK (Immunoblotting test , IBT) tk Ps MEK Se HE He 44 BS EN BEA (enzyme linked im- munoelectrotransfer blot ,EITB) , [14 Southern 9-357 (#9 Al BCP HY EDS H Southern blot FBPAADL tH BAKY Western blot, S#EEUES = BBLUETT LAL 1-3. S8—Br Bt SDS — FRET Aas POE BEBE HTK (SDS-PAGE); 4c DR SPAE APE ih 2 SDS Ab J Jer BA He ta, PER TA RFE BEBE A BP PARR oh, BE A RY Sh FRE DH ERG BR BOR AIR ATT WLR ATER 6a AEH LIK AT) 0 OPES: HERE PE SYA BR RA HG He A EB A ER HR , eR Ha FE (L00V ) AAC Ha BAe (1 ~ 2A) , a ASmnin FERS BUTT SER. HEBERT RS BY I AR AF PAYS AS SL 9S PR Pe EEA A EUAa BEA BA HAT TR DAR) RO SEED BERS BCAA EFI JA BEE RAS PEE £8, YI HER AEA) HRP JRIOD I 3,3" — RIA Me (REE) FAL 4 - Sl — 1 - SEP EEE) PAE AY RAE BaF AT BE, IE ATARYE SDS-PAGE Ff Hm A HOHE A) CE, HE AP SP HR tS BERG T SDS-PAGE (1) 2)98 H7 $5 BIG ID Se EOD DAL SW HE (ELISA ) (1) 455 SE A ABR J AAT ER, ASF IL FF TRA SY BE ETE HE FFT FP BT CTE PEED HERS, RAL KG LE ER A Nv Fe, BC ES ACS B16, Hl CT A PE Ss PE A 8 SH SHARMA SER AB td BET HT Aa EE BE [Xe] ete HUDF ee 224000| — 102000) 169000) 48000] Wied I é 2 ae . Ns es ‘aa (an A re CLES AAT) agin. s.fp Fl -3 SRA RRA (3) BET PLSEH (Ouchterlony) 3k Je — FH He FH AR BA be Be it a AO AY ae HE PEWTER AL PAS TB a DET OSA FEAF CM SIE MRR. WED SIS AT VR} — BAY AR BY ALAS — OZER. Malmheden 2°) Ail AUSL Se iI7 WIM HOM SPOT TRAC A Oe EE Ah AYA . OTE MIAR ARAN ETE T AE AE OT, ABUSE BAK, TT EL LEA SRA (24 ~48h) (4) RS BECD ESM | HATHA Ts See EN as SCO HE PP BP A HE A Sa PO FDO) RARE at CBF A Ae a — TEASER (=i) DEAT SRE AM , BOT EE A EE A TK RR SSE 708 5 TG LAF RAS BEAK (5) KAPRBEBTKI MR STAT ALL ST EE TT PEP BASE ARE a BAS aK a SE AT eR S/R SE EI SRE ARAN TLEE «AAPA TTRE DK 19 2 8 A SPE A PE AE ER. TE AY BEBE AT VA FAG IE tT A BE A RE BG) RIE PA LARC HD IM AGSRIEBEDN “> HL, Malmheden °°! FR] RIE #230 T 4A hh PO AL UAE EB APE ESA HH SO OF ABE RIE AAR AA SE RR EL BE FP il BEANIE C2 PREZ 28 (6) Mi He RE PASE ME 1971 4F, Engvall #l Perlmann &-2¢ 1 RAID $e, EO BAL FA SC Me 9 ESRMAGARARM ATA (enzymelinke dimmunosorbentassay , ELISA.) EJF leG 2 HEIM SE HY SCRE, (849 1966 AE FEMA JIE ICI EARL EAS TAA AR TR AS PE I ET. ELISA FE SE RAPD HTT (ALLA BEEF) ELISA 342 — hm ht BEE, cht Knights PY 56 Sp 24 FAP aA 2k Oe HE, Sampson PEANSP LY T aS IM 5 7k Se TR BL AO, 20 tt#d 90 4E{R AI ELISA 4k AR Ae EIR HE, JZ A BH PRY TLE i, HE ELISA, BOREHAM TR BUR AT TRAE IEG , EAE RO SH A AH. ELISA AUPE AEDE Jeet PS AY TATA AC AUER, E BTR A FY ELISA + SCA BES Ph TE A BR 9 28 TE FAME GURD A 2. PPAR a Sh PR ( a) eR EL PIR IER AE ATR SAS CCR) SAA AT A He TG ACK — SET, ZT AVE ts BEE ASC DER) AS PI TAS OY BE, J TA A A ae BE 5 ELISA §2 (6, a0aih. Jy TEMS EI REED PEAR, ELISA PRT AER pric DMA ALPINE RAR ELISA Py 3 (194F AS ES FEGE— BA PME, HE FH ET 9 PAL = «BL Be: PAE HEANIE HI. AE ABE IgM GLK ABS (avidin biobin system) -ELISA 3% , PCR - ELISA #5 BE RENE Tt ( DIA) 4"), D VaR: TALES SESE MEST PA AG RE A 3 EE BIE TT PEPE AY , BBE E> FAA A UL, ERR, ILA AL BTR RAR, PE BERRIES ILA BRI ETAT COLA yb ASR BE BIN EA ERA IgG IgM) , PERE UE AR Jes UES) 8, PO AE BT, Ba A SPA RT SP Se BE be HWE. Q WTAE: ATE HG th J ET eR, BE MR AAT TREN 65 WM FF th, RE PH PE, 5 eT A a LOR RERKRAA, ART. Mea, MAA iE, ES SPE ET. WEA Jet, IMIS Oy So £6 DEFT Wd aE, IR Be eA at BO I A BE CIM (1 Si Ds aT A AT VA GR AB A, BS — Ee Ud A aR EUSA HEF, iT 55 — sO Eg Bp ES EST PR EA A,B fe FP A a FBR F 5000 By-E i RZ AT HEE 0 REBK: AEA TORE EDT ARID FAA Ze IMT, VERE Js a PA A: — 201 BPR TE AUB BD TL HK, TT Fs — AS eR, PPE a Ec PER ANE BLP , BU BR ATT IT BEA FE 9 AS ATG RB PEPER TT TM ELISA EASE PEF GR A AR GR BET AE DER SORE OT IPS T BEER. BRR AAT RT ih EAR A A PULLER , BUTE BEET RA PH ELISA Dy FF 0 oh eh ABI AE BE AFR aL BASEN P TE) , ELISA LER — A ARS TEE AL Tl AED Be HEMT JE A IZ AR 3. PCR PCR Sb $5 BE AS — A FL HS A BE DG AE PARK) 9 AOC A SEE GY TT AL UB AE , GD PCR Dy eT Bh EB TR (ARSE A Ny BE) OY AY BOL 2+) DB De Be A Ae RT BR AEH GK 10 SR SHUARNBAARR B) 0.001% , 1-2 PCR #2360231 8 cDNA Tit set HRB ‘ah ‘CSO LEE POR WESE(Com 1 92 1R2bp JF) SAFBESEA 30 ACR MER RRM | Aas tony PHM (Holshauser,2000) ARDEA TE POR AAEM (oo - AERA SAREE LIL RHE, MH ARIE 0.001 ~ (1) (Esther, 1998) Pe 0.014/100g eM 4.0.04 ~ 4mg/100g ELISA FY LAS Ei PEA PC AIR ( 4 SUR) AE I PCR PILE RR AA APPEND PREMISE DNA AUFEZE ELIE, PCR SQA AT Bh HF TEE A PEE FRAIL. PCR SALAS HE RRA HRT, WIR BEDE FE BRP TA AY DNA IX SPT LR RAGE 7 ~ 10d, STF — 7B Bate SEAR, ELISA py PSE SEH ER EE RIDE, TL BE — PPE AOL TE]. PCR SEAR SELA TAA REY DNA FFI Ay Rew , TAY ELISA 208% CRA 1 AS aE A) BMA. ITA ih DNA ARAN BEER ERS BY AR, 2 ,PCR RIE BF LAB Hh PLB SE ATT, CERT TMA BK HET 4. DMR B RAMA ESC RB AYRES AUT 19 tHe A 20 Hee AYE AT BA), RK AR RGR MELASMA SAR. SFT TE EE, Ba SG GET TAL IDLH, RBS EAB AG PRA LE ACF Be AI BPE Ds REAL INL Bah A IAAT 2 LL LAA th Da ESE RR HET SERRE WEEE T Bit BE 2 - DE $e Sc PRE Bo hE A SALES AT FGA PR RE TAA PAS EAS VD A AR A BA TS, FF FESR EEA ESE T LA 2 — DE 48 SCPE RG Jy BB UE AR RE AEA, MALDI - TOPMS 48 2x PA iP IE 9 FFT HE , SB aT BERGE SF TSHR, oF PAE BL SEY TTP BY INL FEE TES AY I ER AND BEA Pea BROT ALAR a AULT BB ABR ART A Be I POS crit FR Ce IR. — 9 BE 1. ie Brenna OEE REA , BEF IR AP AYE ART VA IAB DE EAA, FETE FEF RL SPAN SBR Pru p3 (prunus persica 3) fh FHM ACE MARR, Alta a MER] VA il 48 — AER AE ETO) (ELBE EA C09 PF FE TBR Se, " SERMAARAOGM BAER Ae De fl bh ka FPS AE TT EZ EAT 2. PANES SEF i ABD a ea DAN ih, HA Lh ES BR I 2 Wd 4 HUE TT HE AEC YSERA LB A A 4D EB IT AY = PAY, TTT ARDS eB A I A AY , PA PAE SE BS EB BA EK Svenning 4381 814° FLUE TT AALS AT (RAP PLP REO a —LA AB - LC Aye ERE SY BE ARSE FLNY 12. 72% Fil 18. 74%" 3. SRA SRR RL SL ABD i EE A OAS LBL © BER B Sm MLTR REA HS. © WER T ANOLE — BAH BS RPE IL INAS AY FA I — BRB FY SRE AR A: DE AL EUR SE FESR Be IE a 5 24 HY EYRE fal — A OLE LAURE ee ER" DIK” , eRe DEH HE) , ha DRTC «Heb , IRA 2G AS SbF Si SL Fa A A afk SSIS BP OE , CE TL BCH A FAS A Ae SE TE, ABE EE (UE WM BAT FH DH. =. * HK SIME eT ES DOMES FE FEY oe — Ba © — BE BETTIE mR USE ACT GE IN 5 Aa AI BY DP BEG PLB EAR, HEP ACRE) HEM ALY ARB RZ MZ ERI ES RI AA BCI Be A IE A I 5 SP BL AIG He HL AAI i F-Sek ABARG Hy a EA A FATA DSRS A) NA, FD a PF A HW HLH, CB 2 FAUT. Beeb, Ryo Nakamura Al }HAa PS BATE REHEARSE A BRT 90% BL ARC HA, TPA Ra =. EW EAE 1. BBR ‘FEUER hh O52 09 PL RR TE 19 PLDT ZOE, ELISA SUAS BL - FLEE A B ~ FLBR AE BURCHE BEAR 999% , (ELSE HE Be ARSE OP, CB A HE I AS A SE MI”? Je drychowski SESE E51, 4 FLAIR Da Az BES FLIN e AHE H OR BEI T 99% {LB — FLERE Al a — FLA EAE RA APR, PUES T 2. WRK Se BBE RIL FEA BAS ACHE TS At Ph PE HE RE EAE, GRR TRE AM GH SA AA) AS ABE AHR FPR ART: AUS RAAT LL BARE A A 12 SE RVR 3. ALB 32 NEAR, FY VAR A WR ARR BB AEE, Tada SPE SE AE , A HEA FB BY BR SE BRIS , TSP BH A KA A HE IE BE AY 300 .e/ BE RES! 60 ~ 70 e/ BBS EFHERUIE WHE RACE FB 20% 4K!) Herman “Hit , FA AE BIN Hil AI ITE AT DRA SE ETS A Gly m Bd 30K. PR iT, ARAL MT Ra BE LSS Ta) A A A DAL BAS PR HE PHS ag BEL aS Td A HE A A AFHRAABAR DRA”. 1 Se TERRA EE A EET EAS AT DE FEA RACE A EA) TA BER SD ET Pe AS SE FRE AA BEI BEAST A, ARP ELAS TH A BC A = RABE RG RR Ch HERR AREF SHER EA CA FL, REG AE BE A PR PAR AS © TE AR FB PERMEATE HE TEAR Fi BEA FE 19 TE HE ECE REE (PERE) EE RE. AT LAA FCB A BT. 4 > AST a IB 5. SHER A PAB FPA ak ART Bk PE Le 1-3, m1-3 FORRES BARNS HG Lies’ HAMAR TERI TEHE BO TR) FEREAMK( pepsin) FARE AM FFF IEG ( Tyr -, Phe-) SE SA IEMA; Lou -, Arg weak ‘BeHEB( Trypsin) eee Lys — ,Ang - Seats (BSE ILENE ( Chymotrypsin) RT EINE Phe — Tyr , Try ~ S228 ‘BURGER IAI ( Pancreatin ) ae REE ae AAI Hi AI AICHE (#488) papain See Ne ANE Lys ~ ,Ang~ ,Phe ~ #2238 7B HEE Bt EL) papain ARTA, AREAL EAE AMAT MATES D FeAe RE A BM (ficin ) REE Phe ~ , Tyr ~ #8 eit : Lys ~ , Arg ~ , Phe ~ Tyr ~ ‘SB AECTBH( brometin) EIR EE aaa cme ~ AL see YDE I REE SE — EH a ( vankese cealase) Proteinase K ee PYRE A — FLEE Ri ( Corolase ) EERMUAARROMEATA SR FFL A — BPO PL 8 KS PLR RH, ERP — AB PAS SL 200 EBV BP Ta AB A ke BU, Guadix 250°) 449052 CE BE BR — A A PE SOC pH 8.5. BY SAE TF SEAT ACI, SER BEI FLT AC il SCAB HEL 99, 97% . Shimakura 46!) 3 FA oc — BOSEFL TE NESS 3 BPM RT RHE TER GUE TALIM ELISA Sco FR Ae BH, SEE BE it Ae SRR VLER TE WS BE IL BREA ‘Toumura 2° xo DO ROC ya LETT ORE, EEL— FH Proleather FG ~ F RUBRHE REA WEAK fh BEI) 25 % i AEA BC ft Gly m Bd 30K BcC2E A , TE ELIGAY 7S BRAGA o — Iba Ail 28K 684K a8 tt BKC, LS ks BR BE HL Babiker 5°) 8 BIFSE TSB ASCH FI Be ew A A> Gs 0 a 8 TDL, LLL AL HB FNS BEE IE BAERS 100 9% TP Re RE MEL 75% HE BRA BAL I OS EP PLACE AE EAB AT EI FEE GRRL AEP, cS i AR RE BE AST, BGA KEL 7A Hr BEACHES BEA AYE SE. Ema O74 Hs EF My PARTS} FB SF 5000 BAIA BS AS IEPA SH A BOA Be BE a a FD SU 9 Ek Da BARR ARISE, 7° Ht RAS TAD LY FR 3 A ZA SB, A ACF 10000 Bi)ly F- 200000 , UH AE a ORO) xd 5 gH PL BE ST AE ABER ESE AAS), SF Dy Ba AE A RSS 2 SEEK UE — A EE A, EA ERE FET PUTA BE AA FAFA AEE ABA, TE ZK BE BRK SD FE AB 0 BEAD RE SEAR ARS , FUBERRACAPAE Vi ATTEN. PHAR ZA A 1S AE FE 0 LA ‘te PSEC FR AA Tl, RE $8 LEVMRAHAUNBHAR HEA SL ABC] fs Bt Dy a A A FB A a AB AA SH te LAB A Td bE FEATS ABR BE WS 3X BP BE A TE Ry), BR AR 2 BL A SR HE A BHD BNE). HEM TE AHR A We BR AY RAE BSE: FB SIL , LF: 100% ek AACE Pe AA J Be 7A SR a SBR Or 4 J ll = EL A BS LH AA RHE, PE AS OT | PLATES BERL re ELBA 1981 4 Sachs 96 7E2E US Pe BUSS — 4 CE A IF A 4 Peanut — 1 2 fet, Fa (i Ae BT A ET 7A RE (4S EA) 3 FD ARR SIE — i Hn ME YS Ara hi!) Ara h2") Ara h3 | Ara h4 “| Ara hS '! Ara ho“), Ara h7 “)Cefr i RA LE BAMA FCP 90% HALTER Ara hl Al Ava 2, HLA EAE EE Raia ie AEE TABS A ML HE ASD), FR PEE LAH TUE ARAB RP FE et ARPA (cA) Se ET AT Bk A 5 FA SP A A AR PIE CR AS 8, BHM TL A A 2 A FE Hk FB AG BP BE Te TEAS FR ATTRA, DEBE, BRU Hr TAT HCH BE PE RE; HE SH A LAB I BT JRL BL, ZEA AIRS CAR ih IF A HR SB TEA BABE A AS A — SR MEST (—) Be A(A2-1) R2-1 zee Eo RAN eH TST Ai: Ee TEAR seat FS ISLE Me fs FY; Runner, Virgin, Spanish #il Valencia FEA OR A HE A HE La ACER = Be Be eA 0. Olmol/L pH 7. 8 PBS S20) Dit Ae ADS SF ER EAE AM Pharmacia ‘EPH (Glycerin) pa 8 ACRE IAA B HAZE AeA Amresco + =a (SDS) ste Sion ESRMUMRROMAHTA ad cos eA eB. SPST REP ine Amreseo HAR Glycine) Mt Amreseo PURaEZ, —Re( TEMED) Arie RRA ‘$B E(Bromophenol Blue, BPB) tnt Sigma SMM Acrylamide, Aer) 99% Sigma (Urea) snk Sigma UL ALPAOIRERE( N,N" ~ methylenebisaerylamide Bis) sn Sigma J MEARBE( Ammonium persalfate) cas Sigma 75 FRIEW R -250( Coomassie Brilliant Blue) cd Amresco FFIRH( Methanol) Sit ARIANA] DKIBAR( Acetic acid) Sri SORIA A ‘Sen EN Abit PLB CG ALACRA SLSR Sri ALA HELM LCS (30% ) Site SCRE AL ‘pater Se ORE ‘Tween 20 pad Amresco ARETE ERB (NC BL) An Amersham mA FEE (BSA) iy Sigma 4-M-1-2m ped Sigma 3 108 yh Sigma HRP ICA FLLLA TEE @ — chain specifi) £8 IgG Zymed NV ~28( FF) Rk - DL — REAR - P - REARSERE(BAPNA) = “EA tR Sigma #7 aE THK ( dimethyl sulfoxide ,DMSO) Ae Sigma (=) FBKR(A2-2) 2-2 RS RRB AB TR GCL. —208 kro URL teem ew aT SZ-96 Fy ahMA ARI RE Liens ALE SO) =1 fiat ge a Li LRAT SK ~M TOR ah 5-6 5 1866 ART HR 72S basic MR HI HL AE -6450 AIK AE ~8450 stLik( H # IKALABORTECHINIK 4} HA ATTO 238 HA ATIO ZA} 16 SLE LAVARASMBATA ee ROE AAS PTR DY - NB RRA KAT oa DYY ~ 408 Suse eb LRA diosa DYY ~2A fei AHS RAT (2) RBs 1. CERAM TEA TSG FP FB EB FEA: PP SE EAD (+P) 45 Running , Virginia , Spanish All Valencia ( 32 F8)) , 3: 80/5 .4°C WARE FD. 764 {— FEE OLS AO , He 1Oml/g He ‘aM A TE CLASS, F 4 °C He LLIB, PREG 4°C LD 10min (12000 g ) ,LILIE LP WBRAE TE AE BY HE 1: 20( m/V ) HCPA A 20mmol/L BERRA MPF HK ( Lmol/L AL SAFE, PH 7.8) 4° Hew PER 4h Pla F 4°C FHL>(14000 g ,10 min) REV 4° ET 48h Zhe URS FA. 2. CSR ASEM FEA A— 2 2 BEA See RVI TE SRE , BRIBE PR FLAK — PIES 3. CENRBA IE FLEUR 1 BEER 22 TE UA DE SHE IgE FE HEAL gk Ese BIO HY. FURS WATE ER LE BR Ae OA A BLS A ART ETT. AA AR HH — FR We EAU , BLIMP A — FR IS Bs BE BY ABR A kB BPE LIAR AT Tg Sat PRET LA 5 EAT SY SI TEE Se ESAS TH ER BRACE PALA TgE 5 HEIL SBI FDR 19 ER SP, A A BBR BaHHT HN. PE : SRE AB IZ MME SCS min PAIR ER BBE OR A FE CA TE KF S91 TU/ mL) , 5a fa RINE SLB 3 4b 4 A Ta) BE KK CA IgE 7K E 31 ~90 IU/mL) DAME : 7 $5 FL A (A IgE KF <30 IU/mL) , MPA 2-1 SAR. Rat | ram ————— TERESI RB oars 2-1 abt 4. MAH AE SB 10 GAY AEA 3 SAA BAY FF RIE 2 A 0. 4 L/ FF SbF — 20°C VRAD ORFF 0 5. SDS - PAGE HEAR Laemmli!) BAB , WY ETE Ae HS RG SPH, IRI a SE BE 12. 5% , WRAR EMR HE 4. 5% , BOR BE 3. 6% , HEAT SE LAL UK , ABUL BE 10mA , PEA BES 7 LEROAGCRAOMAATA SR BLLAE SEM BORE >, UB KH IM BR Semin JG, FE AE ABE SBR Spe bE, lh BE HK AS pL ERE HL DK ea a Ps HS SSE RE R250 He fs BE A, DE HERE E Arbitl, FA3E FA CELPRO BE IBE MUR ABE AT HAD MT dF 6. BBR RD (1) REE D AERP HE PERL AE WL pric, 45 SDS - PAGE BEREAIAZ)—FE (6mm x Sm) , HEA] ABD A AN Ge AG 2 — TB HEH HH? 30 ~ 60. ming He IKTEHEAY SDS — PACE MEBEHC ER WA Ree OEE OT 20 ~ 30min, PERE FERRELL TE MOLE DAE eB AB BF BE BED» BE BEC AR — HE A — = FR BAR NC BEBE SFE UE, Eh EIB IA (He I APA), HE RARE = BUT” LA Hk 1H OR. AG NC BBE TE Bk , HE BE TL HAL, FT FP HHL, ETL 9OmA/ HRMS 3. Oh, D HARARE MERE T FAS ITE IK R — 250 Ye €6,( [A] SDS — PAGE) , WIRE BRL SR FEE AY NC IR—-GRPS 0}, GP AVES 10B FABLE 4, Sin , BLES Smin WLS ERR ARLE AA BEEN I (2) Sepe EN a D HEFREWIE AY NC EU ILE AK VERRIAVE Smin , FEVER TBST 6 37°C BYE 4 Uc, AEC 15min, ZEAM A Et PAW (10 ~ 20mL) AIF MILF 37°C ARH Lh SEAT ETAL NC IBS, 9A Jes FL ROK Smin, Hf NC WRG PAI HY eH [ (2.5 ~5) PES EE PEA , FED AR ok BI Smin, ff] TBST Yi 3 WK, UK 10min, ¥§ NC HBAS IAPR HRP pic HO BULA IgE ( AibbpicRhAy 1+ 200 HAE) F 37°C BE FF 2h, FEF ALAR AK VERE Smin , TBST YE 3 UW, AEE 10min, @ SBE 21M HERI NC ABUSE RE Ce ICMR HTM £8, (37°C ,35 ~ 40min) , AEE SEG , BUY FPA AAC ERE , HIE IZ HY © ACA ARA MOE FB Rak Mgt NC BE ke FUE MEAL PLT RE (3) FEET BE EL BE PT a HA NC BH D Fe EH HLEKBE BL) :pH 8.3 ff) 2mmol/L Tris - 192mmol/L HAREM 20% HARE, FART 4°C BUS. @ pH 7.4 fi TBS; Tris 1. 2g, NaCl 8. Og, ti LAE 7K 3 1000mL., FH Lmol/L HCI i] pH B74, @ vEYRM (TBST) ; TBS100mL., Tween -20 0. 05mL, @ SAW (AEA ARR) : TBST + 1% BSA(1% BSA TI FH 5% BLARSLBHUAE) o © GERM CK .0. 1% BIEN 10B 0. 5g,45% FRE SOmL., 10% YK MYA 10mL., HAE 7k 40mL., © BER W : ZF 45 mL, DRRAAR SmL, JM ZEHBK 5OmL BAY D PRARTL AA TE PEH CRE A Hl ) 24 — SRL — 1 - 387 30mg, FRE 10mL, SEH 4 ~~ 1 — SEE SCE DE, PDL A TBS HR SOmL., ti FARIA 30% 3 Jk 30pL., 18 SIR RANGA BATA 7. SRAM wl eae) PAA AE A it DCH PR ABA FF 2 A i PE aw A Bak, SEBEL T 4 AM hil Pe BE RS TG TA A OR A PR, LAA GDS J EE , SRP HEML IE 0. 4mL. 5 20pLL HENE IY 20 pg/L HABE HE BY TE HE BEF 37°C EF Oh, (8 TAP PETAR EEA BE HP RS PAD SUE GT EB 8. SERA RNa ES FHSGE] GELPRO BEBE BURST ABE AT BREE HY Si, HES: ot = HRI eet SHEVA 2 = oe AB T° 100% 9. EERREA N-BASRA HME 464: AGEL SDS — PAGE 4) 25 Jai , F¢EN BIR MZ. MG HR Polyvinylidene fluoride ,PVDF) , Fr Fae EDP AT 10% FA REY 10mmol/L ft) 3 - FE REPT HMB [3 — ( Cyclohexylami- no) ~1 ~propanesuhinie acid ,CAPS ] , #28 Ja AR Sire ik R - 250 Hea LAP Ma 50% ft FF RISE £8, FF FH LAR AERA, MR FAY PROCISE49 1 2 5 BW FAR (iC az BEAN — SAE ET =. RBAR 1. ZENRES MO BRANCH T 10 GY AE Ae et AB MTP ABR AS SEE AEA, 7 APE EAR OL BH, BER 2 ARB ABS 1 AT ABE IES , LAE 2-3. FRE HC HE 9 TA GY RS BE HEE SL ALE TT VAP} A BI HE A TL AR AS BR FR GE HEAR. 2-3 LENSES sm eH ei EAR 1 aK - 4 tHE Ree 2 B 2 sates 3 & 50 RE 4 a 25 atte 5 x 2 Wet ee 6 & 48 abet ee 7 a 6 tHE 8 x 2 tHE RE 9 ke a7 ee eae 10 & 2 TRE 2. ARTE GAA SDS - PAGE Fak Hit ARS AR T TEE GORAS F 7642 EEN SDS - PAGE iif, Wn 2 -2 Bias, 764: EA 19 FERMUARAOM BAHAR AHIR YF Ht EE 5) Ahi LE 14000 ~ 66000 , BRT FY ih PBK 29100 FRA ZIb, AAMT, ARIAL APTELE SDS - PACE EARTHS ADL. FASE CELPRO BEBE MURATA SEW Fe HEE TA BAY APF HE SA TPG BARE EU Hy 63000 , 58000 47100 , 41100 37500 35000, 32800 ,30200 ,29100 ,23600 .20800 ,18600 #11 14500, = i 2 Bt Ml (b) 2-2 SDS-PAGE #4ee Ait (a) (©) 3KFE (b) (a) FRED MAIR 5 Ait iM ES (AED 2 Bt 4} 5 27 94000 , 66200 ,45000 35000 ,28500 , 20000 #1 14400.) 1 ~3—FP BIA ALEAE SAR: E> AR TERITE 4 ~7 SRF 4644 ih; Runner Virginia Spanish Fil Valencia 3. FANS BE AY ED aE Gn 2 -2(b) AtzN ,SDS - PAGE fe FAVA AR HEEN BY T PARA HEA ERE EE ANSE FE NLA 2-30) (b) , Se HE4M Hi] SMA RELA 2 -4(c). FED YE RR BY ARF , 35000, 29100 Fil 14400 Fete 2eBU ARATE IR BH , Alte EE BOC A. ASN By ESE A HART S)F HHL 63000 Fil 58000 fy BEA , HAGA 100% ,41000 ,37500 Ail 23600 2 FHI Sl) Hy 80% , HH LASK 5 AAG A Dy HP FBI EE BARC FY. Tf 30300 , 18700 , 47000 , 32800 i 20800 2 Fy WBE BAY SEARCHES TR Se EE a IH BASE AR SE OR AS, EAT SAM HSM, BATTLI T =a al BS BE , NP 2 - 4c) ~ Ce) , REAP PT, BA TA A PE SPP , LE: BR BAS Bz 1M SB}, AR 0 hil SP HR AF HE 3K HBE_- 63000 58000 41000 .37500 Ail 23600 fy Feit RU AE PG , HAE — AP IE A TE He a BAB Pa TAG SHE A BE A JE FE. ‘GFA 2 — 5 8 2 — 4 Poa, VA BE AL AB A a HA RL, WB 9 A FAY BCR Ara hl, KH!) Ara h3 AEA, MIA 2 -S(cl) rar. MEM RAT AT IA SN Bl A So OR BEAT AM HARM, A 2 - 5 (02) SRA, FEA 2-5 (cl ) Se BE EN ak PA IT HB A BN AFTER 2 5 (2) SiREE NS PAEIR , HER T Ara hl Al Ara h3 BFR HE RINT , 9 38 BA AEE YE BT BR. FILS RADHA SHTA ee 1 ees 2 ial @ Oy © P23 EAS RE 4) Ree Es i mH A (aa) (to) AE BE PT a Pia A A se TH fe 8 Pe ARIA) | Co) AME HE ESR 1 ~3—F O AMERUER 23600] @ PA2-4 See CU SIM fal sea (a) FEE A REFS 0 9 A Se PRN) PEE SA ARE <1 Se A ROP e) PEPE TAR SS PAN HE A 13 Se ED A A PRT Cd) RE AES eS Fei SEE IVAN TCADA (©) RAYENMLIA SSFP MEHR 17 ARREST MEIN SHRP HAE AE 21 EEROUSRAM AHR 4 S 6 Pe ee 26 ch) «@ P25 SERRE: ih ee ES (Col) SIRE AA PE HE MLA SE CEPR (02) 318 Te A te SE EN LAE 4 ~ 7 — He Run nner, Virginia, Spanish #1 Valencia 2-4 HARMEN MMT NR 5am : mate NE 7 Ni Sak ems EMRE N gs aa meres ae ee aA aan ene reas : lnm ie Bake RSPP 85-88 (1995) 63000 RSPP Anhi “4 Buschmann RSPP Les (1996) EGREGE EGREGEQ Burks 3 8000 1s0z4s0 Ab = 9/10 QEEG EWG (1995) 41000, Pee RY Dorr © 1~10 Arah3 10/10 Pitt PEEN EN (1999) 37500 TREO TER QOEE 1-10 Ara h 3 10/10 Aaah PEEN EN (1999) GIEETIC GIEEIICIA Rabjohn 35 23600 26-335 Amh3 9/10 IAS s (1999) 4. FELARREA N - MRM AME HAL AYF LA 63000 VBE N - Sv IEARAF IAT Burks WURAY Ara hl HY3% 85 ~88 fic QUERY A) —BC, 5 Buschmann iif Ara hl 3 1 ~4 (ERIE AE Tl , AG PE 4 63000 AYZE AY Ara hl ; AIX 4}F- Hit 58000 AYE AG Burks WAY Ara hi 3 150 ~ 159 Fy RAEIOIE FALE Hy 90% , FY AE AE Ara hl AY WE SBR Hw FA MS EA 22 FIR RAVGRAOMEHTR 41000 37500 #1 23600 fiyZEF43 Ara h3. 5. ERLSRAR ANAS MRE 2-55 PERE AAR A Hibs FH (P< 0.05) Ara hl 47 48 AE AH PADRE SGN 7. 98% .7. 71% 9. 12% 10.91% 11. 02% 11.61% Fil 10. 43% 5 41 3 AHEM: th BRP HERE Sd F DCH SOOO 2 ARE A BE IPG 4 A+ ADE ABI BEAL ABAD BC 41000 FEA SS BAY 4 AE eH BY 3 ES RT 4 37500 AYRE FTE Virginia "}-4r HEIR IY 6. 43% , PETE REP Ar LIED Dy 3.979% 5 HIRED Fit 23600 AVAREA HEH 5. 34% ~ 13. 45% , R2-S HALERBRARRANSE BE i Wa ea He eH = Runner Virginia Spanish Valencia MIFEEILN TH FAG) ORB ORB) k)— kB) Ce) a Samm — %) 63000" 7.98 7-7 9.12 10.91 1.02—«1L61 10.43, 10/10 8000" 2.12 2.21 2.26 1.52 1.47 14s 1.46 10/10 41000" 3.59 3.26 3.09 3.78 4.31 4.2 4.21 8/10 37500" 5.33 3.97 4.45 5.33 6.43 5.25 6.24 8/10 23600" 11.83 5.44 8.12 138. As ARGF | hee 8/0 +P <0.05, =. # Hill 95.7) BRAG AR AEA BE 08) AE a PET A, AT 3. 4% ~ 5.0% HA FRA POD TEBUAE , £0 HF SF ME ANE DR Oy EEL BUR, ZEA: LP AR | AE AR. 1B Fi AC SANE Be AYES GK BAO BEDE RE, AT 4% HY PL A HEA |. TA JHCIESE AP Bal FE BEATE A AiR Aa HE SE ATMO T TEARGURAS F AEE ES SAY SDS - PAGE [Ali%f 3X49 Koppelman 4%!" OTTER IE — BUN. TEAL AY HAE pH 3 ~6.5'7) ASCE LRAT pH 7. 8 AMA We ORE TEA: A ROK Burks 45°") 58RET Ara hl (3819 3Rt CDNA BRAEFE I Wa T Ara hl (HS SLANE FER, FATE FE AeA RL 5} FH it 63000 AY ZEA N — Say REAB AG Buschmann 2%”) (1435 1 ~4 {iL BOER FF —BC, 9 Ara hl BURCH A , AXES BEE 58000. 9 AG Burks FF AY Ara hl HAAS 150 ~ 159 (2A SEAR IF A 90% FLAT NE , FAHEY Ara hl AYER iBT. AED XY 4PF HICH 41000 il 37000 FAR FAHY N - 3) IERIE IG Rabjohn 4°) Je MAYA MSP FI F-42000 AEH], MIMEAP-F CHE 23600 EAA!G Rabjohn 45° Bes AY AINE 4} Feit 25000 BABE N — Sis SEAR FF Fi — 3B , PS AA AE HY kT Ara 3, FEE KAIST Ara hl ESN SCMCE GR4TIO RG T Burks!) Al Rabjohn AY BSEA RE. Ara hl Al Ara bl IP SEU 22 1 (02.83 Dy BG OR AA A AT WEB Ara bl EA Oe Pe HEF. Ara h3 BARC ES 45 ACER AE ECA TA HE 8 A EE ME , S88 AL 332 {NC AHI Me SPR I A — HE PE WB: (res 1 ~ 325) AVL HE ID. ( res 326 ~ 510) HEHE HEIK') , 23600 SAAT ET ALIE Ara h3 (YS 326 (7S IIR, Hy MUR UE Am aR HE 3H 28 fe A BUT Ara h3 ERLE ASE IT. LERDUARARUEAHA Ara hi Ara h2 il Ara h3 J: [Rb°# #20 6S 3 SEA A BABAR OT ELAR ASS IR Soe Ara 1 FAL Ava 3 BAAR SE BE BAS BAC A 33h DE SRE ta a 9 2S | WE? PACA m EET SS ERAS FHA 4 BA AE A it EAT SE ES 2B AK , Ara h2 YAY PARA Ara hl Al Ara h3 7 Jee BEB A. Ay Ara h2 FALSE RE LPAI I A TH , PA FE SBE ETA ATHY AF, Ara h2 BY REAR FE PR AEE RCE AZ, PERSE JE Ara hl ADRS} MECH 58000 FE EAI= AR Ara h3 Zefik,IFAL Ara hi AUREL} FICHE 58000 ZEEE RGAE AY 100% , Ara h3 HARA 80% | ASTER H Ara hl Ail Ara b3 AAR A HLA FAR ABA (-P <0. 05) , BR 58000 ZEA HEBEPG ST PERVEAE AP Ara hl A Ara 3 (HER BES HERP SE EEE fh #35 Roland 45° Ay BASEA ELE — BN, BG SE EA FALE, PEA EARL 20% «FEE BEAR PE SH ERLE St OB Ae HG IMIR 3G EY aA. WO. AW Ay Hi TEE FR HL BE (1 0 DLAI, AS EF a FN BB FD Et, FD EG A he BEA SEG To Ba BS 78 A AT HE Be 5 3 FA ZEAE it PE EE A A HHA: © Ara hl #1—AFI) Ara b3 ALLE ABE EA BORE IF AESUT Ara hl ft) cDNA [AA 58000 ZEA , EA N — AR ABFF INS Ara hl 9 150 ~ 159 {iA RFE INA 90% HEHE. (QD SRA AEA: hs Bh A BRL ZEAE it Fh SE CT RAB I], BD Ara hl Hil Ara h3 Je 3 BE BCA BEA, BSAA AY SE BEE Ae Be ABE Ara h2 BY AREA AE AR FB SE BE AR, 5 EE FMEA MAK. @ "PEM ZEE ah AR Ara hl il Ara h3 fA A ARES IR 3 EAE, fe PE WR FIRF RAN RAZ—. SAG Ara hl HERP ERNIE —. SRS ARE (-) Rae MH (&2-6) 2-6 uate WA HRI ZI ‘Ara hE BiB RAIA AA {#898 AN (Streptavidin, SA) i EMRE! AAT LAS “MLCT BSA) oad Sigma HARP Ri2AY LALA gE (© ~ chain specifi 9 IgG Zymed = FAKE (dimethyl sulforide DMSO) et Sigma 96 FRR. Ma IRIN Costar aE Shere Aes eA AL OF IDE Ae (TMB) ER ‘Sigma 24 SIE LATARAAMBHTA (5) ae 2-7) 2-7 Re (LAE Hh BAUS APT R S21 —1 {EAE HE RE EMDR ARE 1OA #5 RACE HPLC) Shimazu( FE) PURE bee Mini 1240 RSET LAE EBEA Shimazu( 1) BIO ~ RADSSO AHR BIO-RAD 2A ‘PSHSOOA 4 (38 Ei 5 (ALIS ML RRA RS F-32 AER FAME fe“ =. Ew IK 1. AR SRA Fimoc (ALAA Ras KG C — AC SE IE Ee I — Ah I A AAR Fmoc VET RAGE. AAA, ASR ARH EA ARF LB Oe, 22 HPLC 24k, UR PR ee Fimoc FAVA AKEY BUS HSE AN GG, WUE) 2 - 6 SrA. pees Eaenont cen -{ LD | rme—wb-co0-c-{)-0c8-L)-® al ki us—tu—coo-cu—{_ oc) | Fmoc— @8 8 — pt R R Frnoe—nit—€it—connt—L11-coo-cn-€_ ocr )-® | teenth) -coo-en--{ 00%.) | FA2-6 Fimoe [aARDK A ARE HY AEAS IE (1) CAMA TRIER (2) Na — Foc ABBR BEER (3) MNER ERR (4) (2) ~ (3) (S)ARDRPAE Fmoe—9 ~ 351 EAE LERMULARAAM BHAA 2. Pe AK ZH RH a rR 2A ES dF WFR ABT ERP — HPLC ) i EE GT OP BT oT FEE FE Vydac C - 18218TP # (4. Gem x 250em, Vydac 2 7}, HA). VAAL 0. 1% TRA HY IK? ZAMS % ~ 40% /20min Bh BE YEE , HEE 1. OmL/min, Hew YK Ky 220nm, £E thd 3. BERS ( ELISA) 7 UICC HE ATEN TEE 96 FL AR LEAT A) , AFL I HG BEA HAH LE, SHH IMA FEET LTE KA EC SEEDER OT D FAYE 100% = FFE TWA (DMSO) *P , HIP ATH Ey LOmg/ mL, TER GPR HE DY Lmg/mL. FFF - 70% FH Spe/mL AEA (ASF AAO) CBE 96 FLAIR, AFL SOL. @ HO. 1% PBS - Tween #96 FLAK 4 UC, FH 2% BSA/PBS $f fa AE REE LESS A Ai, Sa BP 2h, @ ¥EIKAH 0. 1% ( m/V ) BSA/PBS FB REEL 20 4e/ mL, AFFLIMA SOL WRATH, AC REPT LAL, ACH CPE LATFL TE RT RA @ MASAO. 1% BSA/PBS FRE 4 FAITE AE EE AF UL —Ht) REPT 2h, © WATTS , DETR RUE 96 FLAG 4 UC PR eT IAAL AL eA EC BSA/PBS ff: 1/800 ARE) BFF 2h OBE 96 FLA 4 WC, HMA TMB JY, FL SOL, BLA EEA MA 100mmol/L BEAR 50 pL AER. #6 450nm BEC FMI OD fff. = BRAMTIE 1. Ara hl RAB ‘Ara hl BAA SER SE OH = RT HE, HAS FL Bh 7k ER ER 9 il, Ara hl AYU ELA EKER) NPL 2-7 a, = “SAR EY x WESC ATE RK RE BS) RAAF Ara hl Ze imi AY 10 ~ 22 LEE BE, ABER EADIE PRE BEML Burks 46" 92 RET Ara hl AVSCEA ii Rt cDNA BRIE FEI Ws T Ara hl AY ABR IEA FER ANE 2 —8 PB), HEA: TR I ANAL ej SRS LAS HT BE IR RB, SE 2S = RR i A A} Sy ASD AR A A ALR ELF: Aree hd YSERA ARE AS Sy THE” Fe LESERROANE OAR eave PA2-7 Ara hl 5}F EY R2-8 Ara bl S308) momAE re MRGRVSPLML LLGILVLASV SATQAKSPYR KTENPCAQR 1-40 LoscQQEPDD LKQKACESRE TKLEYDPRCV YDTGATNORH 41 ~80 PPGERTRGRQ PCDYDDDRRQ PRREEGGRWG PAEPRERERE 81-120 RRPSHQQPRK IRPEGREGEQ EWGTPCSEVR 121 ~ 160 FPSRRFSTRY GNOQNGRIRVL QRFDQRSKQF 161 ~200 QNLQNHRIVO TEARPNTLVL PKHADADNIL Viggcgatvr 201 ~240 VANGNNRKSF NLDEGHALRI PSGFISYILN RHDNQNLRVA 241 ~280 KISMPVNTPG QFEDFFPASS RDQSSYLOGF SRNTLEAAFN 281 ~320 AEFNEIRRVL LEENAGGEQE ERGQRRRSTR SSDNEGVIVK 321 ~360 VSKEHVQELT KHAKSVSKKG SEEEDITNPL NLRDGEPDLS 361 ~400 NNFGRLFEVK PDKKNPQLQD LOMMLTCVEL KEGALMLPHF 401 ~440 NSKAMVIVVY NKGTGNLELV AVRKEQQQRG RREQEWEEEE 441 ~480 EDEEEEGSNR EVRRYTARLK EGDVFIMPAA HPVAINASSE, 481-520 LHLLGFGINA ENNHRIFLAG. DKDNVIDQIE KQAKDLAFPG 521 ~$60 SGEQVEKLIK NQRESHFVSA RPQSQSPSSP EKEDQEEENQ 561 ~600 GGKGPLISIL KAFN 601 ~614 2. AAR LA Ara hl SEAR FEIN (He 2-8) BA, AHEM T 23 A Ara hl (HYAKBE, HLA 2-9. BME RED HAMELS BEE ARES Bo PAE LN HS KS TAAL SARE Boo BARWCF A ALA TCI ETA EAA AB WO UTA Te RA ar EERMACRAMMNEATA SPSL AEE AE Hh Fae FA TEU TL aE} RR HP] ASL RF AGRE LTOALS IKE A BR BRILL RL TTT ABD HK BE A — Ft HRP Be A A Hae SS FREE), ADAFE NIH MURAVIDIE Ae Ara hl AKER, FeHG Ae a AAS ey BER I AY FER 4 AY RARER ER I EAT AIDE I RE HG ARE HE A, WY A Sk 3 A PEPER: C HRP AE 8a PRAY C PR EAR ( BUR) AUR AES A KES 2-9 Ara hi AYRKEE ae SOEMIFA eS 1 AKSSPYQKKT 25 ~34 2 QEPDDLKQKA 48-57 3 LEYDPRLVYD 65 ~74 4 GERTRGRQPG 89 ~98 5 PGDYDDDRRQ 97 ~105 6 PRREEGGRWG 107 ~116 7 REREEDWRQP 123 ~132 8 EDWRRPSHQQ 134-143 9 QPRKIRPEGR 143 ~152 10 TRGQFEDFFP 294 ~303 u SYLQEFSRNT 311 ~320 2 NAEFNETRR 325 ~334 13 EQEERGQRRW 344 ~353 14 DITNPINLRE 393 ~402 15 NNFGKLFEVK 409 ~418 16 GTGNLELVAV 461 ~470 a RRYTARLKEG. 498 ~507 18 ELHLLGFGIN 525 ~534 19 HRIFLAGDKD 539 ~548 20 IDQIEKQAKD 551-560 a KDLAFPCSCE 559 ~ 568 n KESHFVSARP 578 ~587 2 PEKESPEKED 597 ~ 606 3. SRR EEE RURAL PROB AA, SITE aE , BED 90% Fea, ARE EMT ET 4S ik ( BOEBIFIIA GERTRGROPG) All 17 5 Aik ( EMRE I A RRYTARLKEG ) (1) 401A FA, NP 2 -8 PL 2—9 Bias, Heft ey AA GP si fRmv AuR/mv FILS CAVSRASMEHTA 1080} 1000} 850 800 750 700 650 35h 430 350} 19} 109} 7S 67 8 ST 14 is 16 17 1 19 20 OS oF ‘et /min @ TS eT oO MN DG Wb 1 iT i 0 a BoM BE V/min © F284 AKT 17 SAAC iE (a) RAEMRFFII A GERTRGRQPG —(b) SAEMRIFFI I RRYTARLKEG EZRVUSRAAMA OTR MS, OTmin Fi TF 113s sey 107au) 600 800 1000 1200 1400 1600 1800 mlz @) +MS, 03min #16 Te 1249.6 12 02; 00: 600 800 1600 1200 1400 1600 1800 mlz © FA2-9 4K (a) AL IT RK (b) ACRE SIE CENCRAOMNEATR 4. Ara hl (FAR RCHME FARE AETE ABB HH EHS BEE BEA Fe, MNP 2 - 10 2A 2-17 RAR, ABH RA AYER PUES 4 Sik, AE ARIE INF 89 ~98 11,C EFC A HUB RREBEW 17 SK, FEA REARIF IVF 498 ~ 507 fi, D AAAI BE BED 11 SK, HERBIE TA Hy HS 311 ~ 320 480, BABA PRAICUAS (Arc LFA ) 5 DR PS RS a 4 i BR SE CIE KR -TANAA BEAN AK RM —TALRF HEEB TAF. BORE I LESS 25 ~34 89 ~98 393 ~402 498 ~507 Ai 597 ~ 606 {i AKAY LIAR TE 60% Wt IF ABE DBIF Fly BS 498 ~ 507 {29 17 BAK 100% , A BE AFF FI 89 ~ 98 (A 4 SAK 87.5% , AM 17 SU SEE PEF A202, Burks °°) BEFEARHY Ara hl Ai 4 NM EBHE PE I Aeti2, FLEE TF) HAE 25 ~34 65 ~74 89 ~98 11498 ~507 1. ADEE EARIE UTES 25 ~34,89 ~98 #1 498 ~507 (AIP ZENS S Burks ANDERE — BAY , AEN F AI TE BS 393 ~402 FL S97 ~ G06 40 A) PEF A210, FE AS RELL A 3 TE GH A tA PE AS AK. 0.0 nas O35 0.30] 029 oo 025 Boar 020 ait os oid 0.10 00s 0s; i OTs s 7 9 113 15 17 19 21 23 Beek we PL2 10 A Eh Ae Ae BU F ei W211 Bese Ni Ae aU EL Ret 020, a us| 03s 016 re oud gon 2 B10 B02 0.08 ols Ley 0.10 004 002 0s . 1s gor ¥ e = 1S 17 19 21 23 S So Be ce 1315 17 19 21 23 P2-12 CFA ETE HA I E De ic PA2-13 ZEA CR A PED AeA EERMUGRROM AHA - ted 0.2] 15} , RF A A, BE FY AE SBS DYE Bz AE 5 5 FUE PE ETT ER Fb RA 5 a SP ADCS EL AR ED BBE. REAR IFFY RRYTARLKEG AY 17 SARS TEPE RELL AIR A H RE , FP AK BA FH SA He TT EL TE, SEERA . DEFER , 11 FPO Bd AY FEA ERENT F RE AVA A , OR ES 144.145 147 ~ 150 fix FA) EAR AR RE PRN, LT PAY IgE AA BSF BE, ORES 152 (it EPS RO AUR IgE B24 AH) BS, BZ in PE FRAN R i MR IgE BAH EK BS a RAST E Agi EE TART AE KE FEE” ABE YY SS ER TE A BAR PEROEVCHE ACE THR DEL a 2B oA PRATT BEBE PS EROS WO. ABE hy Bi © Vir BeAr CE HL HELA, Ara hi 8 25 ~34 89 ~ 98 393 ~ 402 498 ~ 507, 597 ~ 606 fii ABER IE I Dy He ZEAE SCA SS 498 ~ 507 (i289 17 SABE REPEAL. (Q RAEMBIFTA RRYTARLKEG fit) 17 5 fK GEM FA Be 100 EP SE BS BUI , BORE AE HH MR TE , 5 499 fir SS 503 (ie TO TN MI 17 Sk BUREN: 5 502 (007TH SAR AUB) 17 ASK Ig 2-4 AE Ha BAR &, 35 505 {0 PBS ERIC 9 ER ABE th — EB AR, TT 498 SB ET HU 17 SABRE SSR ALL Ara hl 5 499 {ics 503 {UCAS 502 fie Ht 3 EEROUSCRAAMAAAT PON BRAK Ara hl BORCHERT KAP AEM. SBA MT FONE A: SIRE A 2 =. SES AIRE (7) Raat TAB AAD SHERMER. HRB WH 2-16 (=) BRS BWR2-2, 2) BOAR HF R(K2-10) 2-10 ZaARanee aS Eras HE pH 1 Citrate 0. mol/L BAR —FNAL 0. OS mol/L ARN a 2 NaAC 0. 0SmoV/L Z.ARALO. OSmol/L ZAR 5.0 3 PBS 0. 025 mol/L BMRA. — BAI 0. 025 mol/L RAH 67 4 PBS 0. 02 mol/L. BEA — AF 0. O2mol/L. BNR — LN 7.6 5S PBS (NaCl) 0. O2mol/L. RARE MEENA Lmol/L LAE HA 7.6 6 Urea Gmol/L FRA AIO. O2mol/ L RARE MEME 8.2 7 TBs 0. OLmoI/L Tris £10. OLmol/1. A 85 8 Borate(0.95% ) 0. 025 mol/L ARAN O. 025 mol/L. RCE 9.6 9 Borate(1. 9% ) 0. OSmio1/L HARA 0. OSmol/L EAE &6i 10 Na,COs 0. 05mmol/L. BEARS AO. OSrmol/L. RRL 10.8 =. KBE 1. FEA fa AY DATE RE BRA , SHR SE A ZEAE tl th. 170°C BEG 2Omain hel ES ZEA IG A TE, ACTH PAE ZEAE Smin FEI MEAELE , LOO 2K AE AE A= 20min fil BAR AL EME , ARLE TL AA TE HAR. 2. CRAMER HEH: Be SCH hh DP BI CEA A FA TE HE A= FEY LID HE 1OmL/g HE BTA TED BEF 4C HR ECB, PR 4°C FALL 10min( 12000 g ) IRUTIE TF IH. JBAEZE ALS Be 1:20 m/V ) LEAMA 20mmol/L 258 PEE SRE, IF FI SE PEE BE AS Le 2 — 124°C REM 4h, PGF 4°C BP (14000 g , 10min) , EWM ,4C BH 48h ZR eH. 3. EERRSEME FAA — Eo GUE LAVARAM ATA 4. SDS - PAGE ik SRSA RB— WS, 5. ABBR RRA BRPIKB—H 6. MFA ANE BS GPE AB A 9 LT EA BRE A 0. 4 mL PEAY - 20°C TATRA 7. BEERS BH ( ELISA) RFE TL SB Se FE SE AST AG il SCARED) °) ARE DRCEF SL P2 -20,, FEEL BE (SOmmol/L pH 9. 6 fry PUR EE MH) HE AEA AE AA ER LOpe/mL., APE file 3 ATROPHY ZEA EF (AG) A A ic BLE A IgE ( tit.) JE PROP #8 a BM, AE EHCP OD45Onm Hf {A. LA TBS - Tween FL DAB BE (EL 52 NE BCE BE 4 ISIN, EN RH RS SAE FAL PBL AS WT Se HE EA) Sas JTF A J SB £8, YE TMB ie FR) J 10mL 0. 1 mol/L fy pH 6.0 AI BERRZE MH AN 100 p.L TMB Je" I 15 pL fk) 30% 3 AE Age Hl TAR, FE TMB Je YC HE 60mg TMB HFT 1OmL, — FEY BATT A 5 GUE (HRNE<20 ug/L) , #EFL100 nL, CHER ACTA ‘TBS-Tween 2096%R3K, #23 min eee en MAAIBSA (HTBS-Tween 20H 7AWOTCHIA IN, HEFL10ONL, AH (Hiei), HEFLIOORL A SGcAMGAES 7C FE BE TBS-Tween 20963, 23min MARE Bi, AEFL1O0pL. ‘TBS-Tween 20963RS%K, U3ming ARVAPLARK, 43min IRM TMB REA, #EFL100L. BRSUAS HENGE ES~30min, Sie hy tk we M2moV. HSOPEIE LA, BALMS, AEFLSOUL fEI-Smin BERGE (450nm) F220 PGI eae Roa Tic PT EEROUSRRAM BHT =a RR 1. RAAB MEE RADE tw TREAT HE AE AE Je Be Bd 2 FS HEE lh, BG HEA Se EL Hak Sk 2 9 EE ht AD SHAS Sie FLA Fe a HTT TL EE ih, PEER ERE T Se We SE HS FALE) pH 4 ~ 11 9 ARE PEE HR , EE AS VB PE RT ZEA: RH SCG. ANP 2 — 21 SR Aas, AR TE PE WE A He IR AS), A 36. 83 mg/mL, FE 2. 09mg/mL. HAR AN AR AR TEC ARTA 5 7 Ae A RAK, SUG HE pH 3 ~6.5 BARRA AMET ARTE A BE THLE. PBS pH 7.6 (NaCl) EYES FA AF HBL TF PBS ( pH 7. 6) AS 22 PRE MBE II AY , BS AG BSE TBR FEA SF TRE, HEMTHEMS TA ASEH. PBS( pH 7. 6) FEW A & HF PBS (pH 6. 7) SH PEF ROLE HOC 651-2 Hak TPN RSF Pe TE 6) RPP BB SE AS BS KAAPRETRARRBMIAK, HA 4 AEA fl th TE Pe] — Be he PE A BEI BBE FI BA ( P< 0.05) , RAAB, BOB TE Ae BE Ae REA HELIS «FLUO TH ME TE AER JE BENG TEE, TK ACAE AE EA Ee EIBEAIR 10 PREPS IBE PY, RRR 04) SE HE HR HG BONO FARE EAE OP PEE POE IA BE wee make a nite REEL M/(me/mL) Bae gS wa? sed a? a8 gh a? gh oP 22 SP aR” Pos eX CELL ES FS F221 ATR PE RAHI HE A ES AE eB 2. ASEAN Ara hl FA Ara h3 RRR AR TDR PE HOA OCH A lth BHR PGR AP) — 22. Pa, EA A "F(pHI ~2) ,Ara hl Ail Ara h3 EA RPE AIL WS ; te PBS ( pH 7. 6) Bem ve iH SETHE Ara hl f9-& HEBH SB Wh /l> , PBSpH 7. 6 ( NaCl) PARP ZK AEE Ara hl Br BEAR 5 PH 6 ~ 11 fy PBL, 2 A= BE A FEL Tk PEL GPE AAD, Pte ASS LA PE EI HE A BC A RORUEGT T a2 GMiT (12-23). PBS pH7. 6 #il PBS pH7. 6 (NaCl) 384 AEA 36 Soe LANGA STE A TEES @e BESHIRP HEN TEE. ITE pH 8. 2 ~ 10. 8 FEF TIME TE Ae fit ies FEMS TE. 7 PEGI POCA ARENT WCE , AK ACAE AE Ara bil 2G A RED PEI HE A CA OE HE CAE EAE 8 SF AN AB. 2 24). SBE MMA Ara h3 HERBIE FEAR Eo Arh Arah3 ‘Arab Ara h3 3 6 7 8 9 10 PA 2-22 AIRSET TE: BEA SDS - PAGE Flite T= BIRR A ARE IK AEE, | MR 1— FREER EL 2— NARA 3—PBS pH 6.7 aPHEA 4—PBS pH 7. 6 SePAAL S—PBS pH 7.6 (NaCl Sab 6 HURRAH 7—TBS pH 8.5 SEMPER 8 RARER RHF (O. 95% ) 9 — AIRE HEWL(L. 9% | 10—BRRR AMEE (pH 10. 8) LERDIARAGUBAAR RHE mT mitt: BARRE: AUR EMC SHE FA2-23 ASEM AEMERT Ara hl PRAY HERE @ (b) © @ FA2-24 764 Hah REED (2) PRR MEO 7 BE PAE et) RI 8 oe A BE EP (©) BRIE FEAL ( pH 7. 6 ) SENS 7 il i AE PE SE a PEC d) RE FEEL ( pHH 7. 6) BENG 7 A A i EB a A SE a H ~ B—IUBHEM: (TEE) SBE in Bl AE 3. REAM MSR EA Bate i ARTS RY TAS TFL Be EERO AEA: FAR EY BE, ALB — 11, PRATT CAE TANASE MPP EHEIK Ara hl FE A BCSCE AS aE 1C, ATRLSE MARIE Ara b3 HERA MBAR I CP >0.05). SEB SRY : PRR BELAY BUS, BEE SKE AUK ACTEM: A OD {ESP ( bE WO, 792( £0.03) ,0.746( +0. 03) ,0. 814( +0. 03) Hi 0.773( +0.03) ,25R AEE I ( P >0.05). FA PBS pH 7. 6 P2384 0. 787 ( +0. 01) 0. 828( +0. 02) 0. 725( +0. 02) Al 0. 738( +0.01), PANTIE IE FE ABAE( P <0. 05) FEE BERG TEA BRC HEREES TAME TEASER, FRAT MAS LE TREE PBDI HY Be EAT TEETER EHDA Lo BARI APR PE IIE 19 6, BEEN SPA, NPE 2 —24 fra , AeA AST LAI EPPO BIH EE AS So IE EEE TR 38 EK LAAARRAMAAAT B= ra ELBE $90°Le ze Le 660'SE Iss "8 +3 ee Tose RLS oz Ie Lolz ez 07 sve0e 07% sre"81 west es 61 cose z's ons '6 $196 exe's SrL'6 s0r'9 8019 16r'6 oosie so0

    You might also like