Biotechnology and Biochemical Engineering

SCHEME AND SYLLABUS FOR M.
TECH (FULL TIME) DEGREE COURSE
in
BIOTECHNOLOGY AND BIOCHEMICAL ENGINEERING (2015 Scheme)

(Specialization: Biotechnology and Biochemical Engineering)
(Faculty of Engineering)
At
ALAPPUZHA CLUSTER
of the
APJ ABDUL KALAM TECHNOLOGICAL UNIVERSITY
M.Tech (Full Time) Degree Course in

BIOTECHNOLOGY AND BIOCHEMICAL ENGINEERING
1
(Specialization: Biotechnology and Biochemical Engineering)
SCHEME(2015)
SEMESTER I (Credits: 23)
End Semester Exam

Intern
Exam
Course No. L-T-P al Duration Credits
Slot Name of Subject
Marks Marks (hrs)
A Mathematical
03 BH 6001
Applications in 4-0-0 40 60 3 4
Biotechnology
Advanced Bioanalytical
B 03 BH 6011 4-0-0 40 60 3 4
Techniques
Advanced Downstream
C 03 BH 6021 Biotechnological 4-0-0 40 60 3
4
Processing
Genomics and
D 03 BH 6031 3-0-0 40 60 3 3
Proteomics
E Elective I 3-0-0 40 60 3 3
S 03 RM 6001 Research Methodology 1-1-0 100 2
Biotechnology and
03 BH 6801 1
U Biochemical Engineering 0-0-2 100
Laboratory-I
03 BH 6901 2
T Seminar I 0-0-2 100
TOTAL 19-1-4 500 300 23
2
ELECTIVE I
03 BH 6041: Immuno technology
03 BH 6051: Bioprocess Control and Instrumentation
03 BH 6061: Biopharmaceutical Technology
3
SEMESTER II (Credits: 19)
End Semester Exam

Exam Internal Credits
Course No. L-T-P Duration
Slot Name of Subject Marks
Marks (hrs)
Bioreactor Design and

A 03 BH 6002 4-0-0 40 60 3 4
Analysis
Advanced Genetic
B 03 BH 6012 3-0-0 40 60 3 3
Engineering
C 03 BH 6022 Bioreaction Engineering 3-0-0 40 60 3 3
D Elective II 3-0-0 40 60 3 3
E Elective III 3-0-0 40 60 3 3
Biotechnology and
U 03 BH 6802 Biochemical Engineering 0-0-2 100 1
Laboratory-II
V 03 BH 6902 Mini Project 0-0-4 100 2
TOTAL 16-0-6 400 300 19
4
ELECTIVE II
03 BH 6032: Applied Bioinformatics

03 BH 6042: Enzyme Engineering and Technology
03 BH 6052: Biotechnology Resource Planning, IPR and Biosafety
ELECTIVE III
03 BH 6062: Biological Treatment of Waste
03 BH 6072: Nanobiotechnology
03 BH 6082: Plant Biotechnology
5
SEMESTER III (Credits: 14)
End Semester Exam

Exam Internal Credits
Course No. L-T-P Duration
Slot Name of Subject Marks
Marks (hrs)
A Elective IV 3-0-0 40 60 3 3
B Elective V 3-0-0 40 60 3 3
T 03 BH 7903 Seminar II 0-0-2 100 2
03 BH 7913 Project (Phase I) 0-0-8 50 6
TOTAL 6-0-10 230 120 6 14
ELECTIVE IV
03 BH 7003: Advanced Fermentation Technology

03 BH 7013: Modeling and Simulation of Bioprocesses
03 BH 7023: Metabolic Engineering
ELECTIVE V
03 BH 7033: Food Biotechnology

03 BH 7043: Bioenergy and Biofuel Technology
03 BH 7053: Animal Cell Technology
6
SEMESTER IV (Credits: 12)
End Semester Exam

Internal Credits
Exam Slot Course No. Name of L-T-P Duration
Marks
Subject Marks (hrs)
Project
03 BH 7914 0-0-21 70 30 12
(Phase 2)
12
Total credits for Semester IV
7
SEMESTER I
8
Course No. Course Name L-T-P-Credits Year of
Introduction
03 BH 6001 MATHEMATICAL 4-0-0-4 2015

APPLICATIONS IN
BIOTECHNOLOGY
Course Objectives
This course provides an overview of the mathematical methods used in the analysis of
biochemical systems and processes and forms the basis for modelling and optimization of
biological processes.
Syllabus
Modeling as a mathematical tool to investigate biological systems, Differential equations,
Regression analysis, Graph theory and networks.
Expected outcome
Students who successfully complete this course will be able to represent a biological problem in
terms of differential equations (models), simplify and solve the differential equations, understand
the solution in biological terms and use models to optimize and control biological processes.
References
1. Peeyush Chandra and Rathish Kumar, B.V., Mathematical Biology, Anshan Publishers Ltd.
2. Demin, O. (2009). Kinetic modelling in systems biology. Champman and Hall/CRC.
3. Jones, D. S. (2010). Differential equations and mathematical biology. CRC Press.
4. Bailey, N. T. J., Statistical methods in biology. New York: Cambridge University Press.
9
Course Plan
Module Content Hours Semester

Exam
Marks
I Modelling as a mathematical tools to investigate 16 25%

biological systems, state variables and model
parameters, linear and non-linear relations,
deterministic models and stochastic models,
biochemical reaction networks, closed and open
networks, simple network examples, time scales and
model reduction, S-system modelling, examples from
biological systems.
First Internal Exam
II Differential equations - homogeneous and 16 25%

inhomogeneous differential equations, development of
differential equations using examples from biological
systems and elementary reaction kinetics, numerical
simulation of differential equations, phase plane
analysis for a linear first-order ODE, bifurcation
diagram for a first-order linear ODE, stochastic first-
order differential equations (SDEs), The Langevin
equation, theory of partial differential equations in
biological systems.
III Method of least squares, approximation theory, linear 15 25%

approximation of a function of a single variable,
(parametric) sensitivity analysis, global and local
sensitivity analysis, determination of local sensitivity
coefficients, parameter fitting, simple linear regression,
least-square fit, testing for goodness of fit
Second Internal Exam
10
IV Introduction to graph theory and networks - graph 15 25%
theory and definitions, using graph theory to analyse
biological networks, data handling and analysis,
smoothening, interpolation, type of errors and
calculation of errors, states and parameter estimation,
measures of central tendency, measures of dispersion
probability distributions, average, variance, standard
deviation and standard error, elements of a statistical
test, F-test for equality of variances, significance tests,
chi-squared test, statistical association between two
variables, correlation coefficient, linear regression, one
way analysis of variances (ANOVA).
End semester Exam
11
Introduction
03 BH 6011 ADVANCED BIOANALYTICAL 4-0-0-4 2015

TECHNIQUES
Course Objectives
To provide the advanced knowledge of spectroscopic instrumentation and methodologies, and
with the capability of associating the most appropriate technique to the analytical problem on
hand.
Syllabus
Spectroscopy Techniques, Electrophoretic techniques, Biosensors, Microarrays, Chromatography
Techniques, Radioisotope Techniques
Expected outcome
This course helps the students to acquire knowledge on different bioanalytical techniques.
References
1. Freifelder D., Physical Biochemistry, Application to Biochemistry and Molecular

Biology, 2nd Edition, W.H
2. Spectrometric identification of organic compounds. Silverstein, Webster and

Kiemle (for problems in NMR, MS) 6th ed. 2004.
3. Practical Biochemistry, Principles and Techniques, Keith Wilson and John Walker
4. Modern Spectroscopy, J.M. Hollas, John Wiley and Son Ltd.
5. Principles and Techniques of Biochemistry and Molecular Biology, Wilson K. and

Walker J., Cambridge University Press (2005) 6th ed.
12
Course Plan
Module Content Hours Semester Exam

Marks
I Electrophoretic techniques: Basic principles of 16 25%

electophoresis, Factor affecting electrophoresis,
electrophoretic mobility, paper and gel
electrophoresis, native and denaturing PAGE,
isoelective focussing, pulse field gel electrophoresis.
Western blot analysis, visualization of proteins in
gels, electrophoresis of nucleic acids using agarose
gel, sequencing gel, FIGE, CHEF, denaturing agarose
gel electrophoresis for RNA, capillary electrophoresis
First Internal Exam
II Chromatography Techniques: TLC and Paper 16 25%

chromatography, HPTLC; Chromatographic methods
for macromolecule separation - Gel permeation, Ion
exchange, Hydrophobic, Reverse-phase and Affinity
chromatography; HPLC and FPLC; Criteria of
protein purity
Radioisotope Techniques: Basic physical principles,

the definition of radioactivity, Methods for the
preparation of radiolabelled substances, Methods for
detection of radiation, RIA methods and their
application in analytical chemistry, Immunochemical
analytical methods, The basic principles of the
method, Preparation of polyclonal and monoclonal
antibodies, EIA, ELISA and other immunoassay
techniques.
13
III Spectroscopy Techniques: Buffers; Methods of cell 15 25%
disintegration; Enzyme assays and controls;
Detergents and membrane proteins; Dialysis,
Ultrafiltration and other membrane techniques UV,
Visible and Raman Spectroscopy; Theory and
application of Circular Dichroism; Fluorescence,
NMR, PMR, ESR and Plasma Emission
spectroscopy, Mass spectrometry, components of
mass spectrometer, methods of ionization and mass
analysis including MALDI-TOF
IV Biosensors: Principles and applications of 15 25%

electrochemical, thermometric, optical and
piezoelectric biosensors. Glucose biosensors.
Microarrays: Basic principles. Introduction to
different types. Methods of manufacture.
Applications – differential expression, SNP analysis.
End semester Exam
14
Introduction
03 BH 6021 ADVANCED DOWNSTREAM 4-0-0-4 2015

BIOTECHNOLOGICAL
PROCESSING
Course Objectives
To develop skills of the students in the area of downstream processing with emphasis
onpurification of products.
Syllabus
Role and importance of downstream processing in biotechnological processes, Physio-chemical
basis of Bio-separation process, Enrichment Operations, chromatographic techniques, Product
Polishing& finishing Operations.
Expected outcome
At the end of the course, the student would have learnt about various methods to obtain pure
proteins, enzymes and in general about product development R & D. This will be handy for
projects of Industries.
References
1. Asenjo.J.M, Separation Process in Biotechnology, Taylor & Francis, 1990

2. Bioseparations Science and Engineering, Day, Trevor G, and Harrison, Roger G, and
Rudge, Scott R, Publisher: Oxford University Press, USA, 2002
3. P.A. Belter, E.L. Cusslerand Wei-Houhu – Bioseparations – Downstream
Processing for Biotechnology, Wiley Interscience Pub. (1988).
4. R.O. Jenkins, (Ed.) – Product Recovery in Bioprocess Technology – Biotechnology
by Open Learning Series, Butterworth-Heinemann (1992).
5. Handbook of Bioseparations, SatinderAhuja, Published by Academic Press, 2000.
6. HPLC of Biological Macromolecules, Karen M. Gooding, Fred E. Regnier, Contributor
Karen M. Gooding, Fred E. Regnier, Published by CRC Press, 2002.
7. A Century of Separation Science, Haleem J. Issaq, Published by CRC Press, 2002
15
Course Plan

Exam
Marks
I Role of Downstream Processing In Biotechnology: Role 17 25%

and importance of downstream processing in
biotechnological processes. Problems and requirements
of bio-product purification.Separation characteristics of
proteins and enzymes – size, stability & other biological
properties; Selection of purification methodologies
Synthesis of downstream processes-process synthesis,
mathematical programming techniques, Heuristics,
Artificial Intelligence/ Expert systems, Evolutionary
methods Downstream process case studies- Purification
of human insulin and Streptomycin
Economics of downstream processing in
Biotechnology, cost-cutting strategies, characteristics of
biological mixtures.
Physio-chemical basis of Bio-separation process

(Primary Separation & Recovery process)
Cell disruption methods for intracellular products- Cell
disruption by homogenizer-mechanism, process design
considerations, scale up. Bead mill disruption-operating
parameters, mixing characteristics, economic
consideration, - chemical permeabilization, Enzymatic
lysis, mathematical models, methods, process design
simulations, Sonication, removal of insolubles, biomass
(and particulate debris) separation techniques,
flocculation detailed study of the electro-kinetic
phenomena-numerical problems and sedimentation,
centrifugation and filtration methods (pre-treatments,
filtration theory, continuous rotary filters).
16
First Internal Exam
II Enrichment Operations (Membrane based 16 25%

purifications): micro and ultra-filtration theory, design
and configuration of membrane separation equipment,
applications. Reverse osmosis; Dialysis; Diafiltration ;
Pervaporation; Perstraction
Precipitation methods (with salts, organic solvents, and
polymers, extractive separations, aqueous two-phase
extraction, supercritical extraction); configuration for
stage-wise contacting.Insitu- product removal /
integrated bio-processing
III Product Resolution/Fractionation: Adsorptive 15 25%

chromatographic separation processes,Detection
methods, analysis of chromatographic processes-
Gaussian solutions, staged models, Newtonian
Continuum mechanism and linear equilibria, van
Deemter equation, gel partitioning model. Gel
permeation chromatography, reversed phase and
hydrophobic interaction chromatography, displacement
chromatography, radial flow chromatography, hybrid
separation technologies (membrane
chromatography,electro chromatography etc) scale up
strategies. Electrophoresis separation processes (all
electrophoresis techniques including capillary
electrophoresis)
17
IV Product Polishing& finishing Operations: 14 25%
Crystallisation, Principles of crystallization and
equipment, yield calculations; Principles of drying;
EMC-RH data, drying curves, various types of
industrial dryers and their criteria for choice and
lyophilization, Freeze drying technique andits
advantages over other methods.
End semester Exam
18
Introduction
03 BH 6031 GENOMICS AND 3-0-0-3 2015

PROTEOMICS
Course Objectives
Genomics and Proteomics deals with the molecular structure of proteins and nucleic acids. It is
also concerned with the structural –functional relationship of biomolecules and how alterations
in their structures affect their function.
Syllabus
Physical mapping Techniques, Gene finding; annotation, Protein level estimation, protein-
protein interactions.
Expected outcome
An in depth study will help the students to understand the mechanism of origin of various
diseases and thereby drug designing and therapy.
References
1. Cantor, C.R. and Smith, C.L. Genomics. The Science and Technology Behind the human
genome project, John Wiley & Sons, 1999.
2. Pennington, S.R. and Dunn, M.J. Proteomics: From protein sequence to function, Vina
Books, 2002.
3. Liebler,D.C. Introduction to Proteomics: Tools for the New Biology, Humana Press,
2002.
4. Hunt, S.P. and Livesey, F.J. Functional Genomics, Oxford University press, 2000
5. Primrose, S.B. Principles of genome analysis : A guide to mapping and sequencing DNA
from different organisms, 2nd ed., Blackwell Science, 1998.
19
Course Plan

Exam
Marks
I Genomes of Bacteria, archae and eukaryote. Physical 15 25%

mapping Techniques: Top down and bottom up
approach; linking and jumping of clones; genome
sequencing; placing small fragments on map; STS
assembly; gap closure; pooling strategies; cytogenetic
mapping techniques.
First Internal Exam
II Gene finding; annotation; ORF and functional 14 25%

prediction; Subtractive DNA library screening;
differential display and representational difference
analysis; SAGE;TOGA
III Protein level estimation; Edman protein 15 25%

microsequencing; protein cleavage; 2 D gel
electrophoresis; metabolic labeling; detection of
proteins on SDS gels; pattern analysis; Mass
spectrometry- principles of MALDI-TOF; Tandem MS-
MS; Peptide mass fingerprinting.
IV Post translational modification; protein-protein 15 25%

interactions; glycoprotein analysis; phosphoprotein
analysis.
End semester Exam
20
Introduction
03 BH 6041 IMMUNOTECHNOLOGY 3-0-0-3 2015
Course Objectives
To understand the components and function of the immune system, techniques for diagnosis and
detection of pathogens, immune system disorders and vaccine development.
Syllabus
Introduction to Immunology, Immuno diagnostic techniques, Tissue typing methods for organ
and tissue transplantations in humans, Complement system, Common immunizations.
Expected outcome
The course will enable the students to understand the immune responses of the human body
caused by applications of bio-incompatible materials and health care product.
References
1. Kuby, J.; Immunology
2. Abdul, K. Abbas, Andrew K. Lightman, Jordan S. Pober.; Cellular and Molecular

Immunology
3. Goding, J.V.; Monoclonal antibodies: Principles and Practice
4. Sringer, T.A.; Hybridoma technology in the Biosciences and Medicine
5. Stites, D.P., Stobo,J.D., Fudenberg, H.H. and Wells, J.V.; Basic and clinical Immunology
6. Ivan Roitt, Jonathan Brostoff and David Male; Immunology
7. Paul W.E.; Fundamentals of Immunology
8. Harlow and David Lane; Antibodies: A laboratory Manual
21
Course Plan

Exam
Marks
I Introduction to Immunology, cells and organs involved in immune 16 25%

system, Antigens-structure and properties, Antibodies - Fine
structure, heterogeneity, mechanism of heterogeneity; types and
subtypes and their properties, Antibody engineering.
First Internal Exam
II Immuno diagnostic techniques: agglutination, precipitation, 16 25%

complement fixation, immunofluorescence,
immunoelectrophoresis, ELISA (Indirect, Sandwich, Competitive,
Chemiluminiscence, ELISPOT assay), radioimmunoassay, western
blotting, flowcytometry, immuno electron microscopy.
III Different types of hypersensitivity reactions, Immunohaematology 15 25%

- blood groups- ABO and Rh, blood group incompatibilities, Tissue
typing methods for organ and tissue transplantations in humans,
mechanisms of graft versus host reaction and rejection;
Immunology of HIV infection and immune evasion.
22
IV Major histocompatibility complex, Complement system - structure, 15 25%
components, properties and functions of complement.Role of
cytokines and hormones in immunomodulation.
Common immunizations- active and passive methods;
immunological preparations-toxoids, antisera, polyclonal and
monoclonal antibodies; vaccines- conventional and modern types
of vaccines.
End semester Exam
23
Course No. Course Name L-T-P-Credits Year of Introduction
03 BH 6051 BIOPROCESS CONTROL AND 3-0-0-3 2015

INSTRUMENTATION
Course Objectives
To understand the fundamentals of process control as applied to bioprocesses in the industry.
Syllabus
Bioprocess control and instrumentation, Biosensors, Data analysis, Biosensors, Control of fermentation, Advanced fermentation
control.
Expected outcome
Small changes in a process can have a large impact on the end result. Variations in proportions, temperature, flow, turbulence,
and many other factors must be carefully and consistently controlled to produce the desired end product with a minimum of raw
materials and energy. Process control technology is the tool that enables manufacturers to keep their operations running within
specified limits and to set more precise limits to maximize profitability, ensure quality and safety.
.
References
Shuler M. L. and Kargi F, Bio-process Engineering, 2nd Edition, Prentice Hall of India, New Delhi, 2002.
Donald R. Coughanowr, Lowell B. Koppel; Process system analysis and control.
Bailey J.E and Ollis D.F, Biochemical Engineering Fundamentals, 2nd Ed., McGraw-Hill Publishing Co.
Yang, V.C and Ngo T.T, Biosensors and Their Applications, Kluwer Academic/Plenum Publishers, 2000.
Stephanopoulose G. Chemical Process Control, An introduction to theory and practice, Prentice Hall of India, New
Delhi, 1993.
Seborg E and Edgar J.F and Mellichamp, Process Dynamics and Control, John Wiley.
24
Course Plan

Exam
Marks
I Monitoring of bioprocess: different types of 16 25%

fermentation-common measurements and control
systems, additional sensors, redox, airflow, weight,
pressure.Online data analysis for measurement of
important physico-chemical and biochemical
parameters.
First Internal Exam
II Methods of on-line and off-line biomass estimation. 16 25%

Flow injection analysis for measurement of substrates,
products, and other metabolites-Data analysis-state and
parameter estimation techniques for biochemical
processes-biosensors in bioprocess monitoring,
biosensors based on thermal effects, optical effects,
potentiometric biosensors, amperometric biosensors,
enzyme electrodes, transducers, electrochemical probes.
25
III Control of fermentation; requirement of control, nature 15 25%
of control, control loop strategy, typical fermentation
sensors, control action, types of control, feedback and
feed forward control loop, different types of
controllers,P,PI,PD and PID.
IV Controller characteristics and tuning, ultimate gain 15 25%

method, cascade control system- fermentation control
system objectives-fermenter control specification,
control of incubation, specification for incubation
control, advanced incubation control-fermentation
profile-other advanced fermentation control.
End semester Exam
26
Introduction
03 BH 6061 BIOPHARMACEUTICAL 3-0-0-3 2015

TECHNOLOGY
Course Objectives
The course aims to teach the students about the interactions and mechanisms of drug molecules in the human body.
It will give an idea about drug composition, packaging, manufacture, physicochemical properties, toxicology,
therapy, and ethical applications.
Syllabus
Pharmaceutical industry and development of drugs, Bulk drug manufacturing, Quality management, Therapeutic
categories, Toxicology, Biotransformation of drugs, Monoclonal Antibody Based Pharmaceuticals.
Expected outcome
This course helps the students to acquire knowledge on biological based drugs and how it can be applied to
pharmaceutical industry.
References
1. Remington, “The Science and Practices of Pharmacy”, 21 st edition, Volume I and II, Lippincott Williams
and Wilkins publishers, 2006.
2. Goodman and Gilman, “The Pharmacological Basis of Therapeutics”, 11 th Edition, McGraw Hill publishers,
2005.
3. P. N. Bennett, M. J.Brown, “Clinical Pharmacology”, 9th Edition, Churchill Livingstone, 2003.
4. Gary Walsh, “Pharmaceutical Biotechnology-Concepts and Applications”, Wiley, 2007.
5. D. J. A. Crommelin, Robert D. Sindelar, “Pharmaceutical Biotechnology,” 2 nd Edition, Taylor & Francis
Publishers, 2004.
6. K. D. Tripathi, “Essentials of Medical Pharmacology,” 6 th Edition, Jaypee publications, 2008.
27
Course Plan

Exam
Marks
I Introduction: Pharmaceutical industry and development of 16 25%

drugs, Types of therapeutic agents and their uses, Economics
and Regulatory aspects.
Drug delivery system: Basic concepts and Novel advances-

Cell specific drug delivery, Brain specific drug targeting
strategies and Pulmonary delivery systems.
Drug action, Metabolism and Pharmacokinetics: Mechanism

of drug action, Physico-chemical principles of drug
metabolism- radioactivity, Pharmacokinetic action of drugs
on human body.
First Internal Exam
28
II Important Unit Processes and their Applications: Bulk drug 16 25%
manufacturers, Type of reactions in bulk drug manufacture
and processes, Special requirement for bulk drug
manufacture.
Manufacturing Principles: Compressed tablets, Wet

granulation-Dry granulation or Slugging-Direct compression,
Tablet presses, Formulation, Coating, Sustained action
dosage forms-parental solution, oral liquids, injections,
ointment, vegetable drugs, topical applications. Preservation
of drugs, Analytical methods and other tests used in drug
manufacture, Packing techniques, Quality management-
Standard of hygiene and Good Manufacturing Practice.
III Therapeutic categories: Laxatives, Analgesics, Non-steroidal 15 25%

contraceptives, External Antiseptics, Antacids, Antibiotics,
Biologicals, Hormones, Vitamins.
Toxicology: Dose response relationship, Risk and its

assessment, Spectrum of undesired effects, Types of toxic
reactions, Toxicity tests in animals, New drug approval
process and clinical trials design-phase 1, phase 2 and phase
3.
29
IV Biotransformation of drugs: Fermentation, Microsomal and 15 25%
non-microsomal mechanisms and Enzymes involved.
Biological Products: Properties of biotechnology derived

therapeutic products, Production of Human insulin,
Interferons, Human growth hormone, Somatotropin,
Somatostatin, Gene Therapy, Vaccines, Monoclonal
Antibody Based Pharmaceuticals, Recombinant Human
Deoxyribonuclease.
End semester Exam
30
Introduction
03RM6001 Research Methodology 1-1-0-2 2015
Course Objectives:
 This course is designed to familiarize the student with the research process, problem identification strategies and formulation of a research
plan by doing case studies
Learning Outcomes:
 Students will be able to write a review paper after critically evaluating the state of the art development in a topic of interest
 Students will acquire capability to write a research proposal in the form of a technical paper which could lead the student towards his / her
final thesis topic
 No formal end semester examination is intended – Evaluation is based on internal oral presentations and a Technical Report or a
Research Plan or a Review Paper
----------------------------------------------------------------------------------------------------------------------------------------------------------------
Syllabus:
---------------------------------------------------------------------------------------------------------------------------------------------------------------
MODULE I
Introduction to Research Methodologies - Objectives -motivation in research- Significance of research - interaction between industries and
research units –research and innovation
Research Formulation- - literature review–

----------------------------------------------------------------------------------------------------------------------------------------------------------------
MODULE II
Ethics in research: – copy right – plagiarism – citation – acknowledgement
----------------------------------------------------------------------------------------------------------------------------------------------------------------
MODULE III
Research Design – and Report writing
MODULE IV
Case Studies : Department / stream specific case study
and preparation of a research plan or a review paper
----------------------------------------------------------------------------------------------------------------------------------------------------------------
References
1. R. Paneersalvam, “Research Methodology”,Prentice Hall of India Pvt. Ltd.,2011

2. Mike Martin, Roland Schinzinger, “Ethics in Engineering” ,McGraw Hill Education, Fourth
Edition,.2014
3. Vinod V Sople,” Managing Intellectual Property-The Strategic Imperative, EDA”, Prentice of Hall
Pvt. Ltd.,2014
4. Kothari C R &Gaurav Garg – “Research Methodology- Methods and Techniques”,New Age
International(P) Ltd Publications,2006
5. Day A Robert,”How to write and publish a scientific paper”,Cambridge University,UK,2012
6. Leedy P D,”Practical Research-Planning and Design”, Prentice Hall of India Pvt. Ltd.
31
Course Plan
Module content Hours Semester
Exam
Marks
I Introduction –Need for research- objectives and motivations in research- 4 25%

Significance of research - -need for interaction between academic institutions, industrial and
research establishments – research and innovation.
Research Formulation- Identifying a research problem- - literature review– confirming to a

research problem based on literature review.
First Internal Exam -- Oral Presentation Part -I
II Research Ethics – Environmental impacts – Ethical issues - Intellectual Property Rights – 3

Patents – legal formalities in filing patent in India – Copy right– plagiarism – citation and
acknowledgement.
III Research design –Prepare research plan. 3 25%

Report writing – types of report – research report, research proposal, funding agencies for
research concerned to the specialization, significance of peer reviewed articles and technical
paper- - simple exercises - oral presentation
Second Internal Exam – Oral Presentation –Part II
IV Case Studies 6 50%

The student is expected to prepare a research plan relating to a topic of current interest in the
concerned specialization, which has appeared in a recent journal. A minimum of 20 related
referred articles should be critically studied. On the basis of this, the student is expected to
prepare a review report/paper of publishable quality.
This paper has to be presented for open defence before the departmental committee. (This
would carry 50% marks)
End semester Evaluation of submitted Technical Report by the departmental committee.
32
03 BH 6801 BIOTECHNOLOGY AND BIOCHEMICAL 0-0-2-1 2015

ENGINEERING LABORATORY-I
Course Objectives
The lab provides hands on experience to biochemical engineering experiments with the ability to plan and execute experiments,
analyze the data and arrive at conclusions, which will equip students to handle complex experiments fundamentals to many areas
of Biotechnology.
Syllabus
Chromatographic techniques and Fermentation experiments.
Expected outcome
Students will prepare, perform, observe and analyze results of biochemical experiments independently.
References
1. Scragg A.H.; Bioreactors in Biotechnology , ellisHorwood Ltd , England
2. Resnick; Process analysis and design for Chemical Engineers
3. Sadavisam and Manickam, 2008. Biochemical methods, New age international publishers.
4. John M Walker, 2002. The protein protocol handbook, Humana press
5. Ghasem D. Najafpour; Biochemical Engineering and Biotechnology
6. Juan A. Asenjo; Separation Processes in Biotechnology.
7. Roger G. Harrison, Paul W. Todd, Scott R. Rudge, DemetriPetrides; Bioseparations Science

and Engineering
33
EXPERIMENTS
1. Affinity Chromatography for purification of Horse radish peroxidase using concanavalin

Agarose columns
2. Gel Chromatography for separation of plant pigments and qualitative analysis of the plant
pigments.
3. Ion Exchange Chromatography for purification of lysozyme using CM cellulose column
4. Protein purification using ammonium sulphate precipitation and Desalting by dialysis
5. Quantification of biomolecules using UV Spectrophotometer
6. Thin Layer Chromatography for separation of amino acids
FERMENTATION EXPERIMENTS
7. Batch sterilization design and Thermal death kinetics
8. Kinetics of growth in a batch reactor.
9. Immobilized enzyme reactor
10. Estimation of Monod Kinetics and yield coefficient calculation using:
A) Submerged fermentation
11. Volumetric oxygen transfer coefficient calculation in the fermenter .
12. Model simulation using MATLAB-SIMULINK, BMS and ISIM software packages
(Minimum of 12 experiments should be performed in the Laboratory)
Marks:
Continuous evaluation: 100 Marks
i) Practical Records /outputs 40%
ii) Regular Class Viva-Voce 20%
iii) Final Test (Objective) 40%
34
Introduction
03 BH 6901 SEMINAR-I 0-0-2-2 2015
Students have to register for the seminar and select a topic in consultation with any faculty
member offering courses for the programme. They are required to choose a topic of their interest
from Biotechnology and Biochemical Engineering related topics preferably from outside the
M.Tech syllabus and give a seminar on that topic. A detailed write-up on the topic of the
seminar is to be prepared in the prescribed format given by the Department. The seminar shall be
of 30 minutes duration and a committee with the Head of the department as the chairman and
two faculty members from the department as members shall evaluate the seminar based on the
coverage of the topic, presentation and ability to answer the questions put forward by the
committee.
Marks:
Seminar Report Evaluation: 50 Marks

Seminar Presentation and evaluation by the committee: 50 Marks
35
SEMESTER II
36
Introduction
03 BH 6002 BIOREACTOR DESIGN AND 4-0-0-4 2015

ANALYSIS
Course Objectives
To provide an understanding of the basic principles of the design of reactors for bioprocesses,
develop mathematical descriptions of reaction kinetics and their relationships with reactor design
and use them to analyze their behavior, and introduce scale-up and scale-down concepts.
Syllabus
Overview of bioreactors, Biochemical aspects of bioreactor design, Analysis of bioreactor
performance, Scale-up and Scale-down of bioreactors.
Expected outcome
Students who successfully complete this course will be able to select a suitable bioreactor and
mode of operation for a bioprocess, apply mass/energy balances and reaction kinetics for the
design and analysis of bioreactors and use scale-up and scale-down considerations for bioprocess
intensification.
References
1. Tapobrata Panda, Bioreactors - Analysis and Design, Tata McGraw-Hill Education.
2. Michael L. Shuler, Fikret Kargı, Bioprocess Engineering: Basic Concepts (2nd Edition),
Prentice Hall.
3. James Edwin Bailey, Biochemical Engineering Fundamentals, McGraw-Hill.
4. Alan H. Scragg, Bioreactors in biotechnology: a practical approach. Ellis Horwood

Limited.
37
Course Plan

Exam
Marks
I Overview of bioreactors - Classification of bioreactors, 16 25%

major components of a typical stirred tank bioreactor
with functions, basic features of special purpose
bioreactors such as membrane bioreactors, perfusion
bioreactors, pulsating column bioreactor,
photobioreactors, bioreactors for animal and plant cell
cultivation, microbioreactors, bioreactors for
immobilised enzymes and cells, bioreactors for
environmental applications, mechanical aspects of
bioreactor design.
First Internal Exam
II Biochemical aspects of bioreactor design - 16 25%

Stoichiometry of bioreactions, mass balances for
bioreactors, yield factors, application of yield factors to
arrive at single-carbon and energy yielding substrate,
biomass and product formation, and nitrogen and
oxygen requirements, experimental determination of
yield factors, distinction between observed yields and
true yields, factors influencing yield, degree of
reductance of substrate and its influence on yield
coefficient, general energy balance in bioreactors,
numerical problems.
38
III Analysis of bioreactor performance - Development of 15 25%
performance equations for ideal batch, CSTR and plug
flow reactors, non-ideal behaviour in bioreactors,
models for non-ideal reactors, prediction of conversion
in non-ideal chemostat, transient behaviour in
bioreactors, stability of bioreactors, phase-plane
analysis, bifurcation analysis, numerical problems
IV Scale-up and Scale-down of bioreactors - strategies and 15 25%

methods for scale-up, similarity criteria, Hubbard
method, method of Wang et al., Ettler’s method,
dimensionless numbers and scale up, scale up based on
aeration and power requirement (Aeration and Power
number), regime analysis and the scale-down
bioreactor.
End semester Exam
39
Introduction
03 BH 6012 ADVANCED GENETIC 3-0-0-3 2015

ENGINEERING
Course Objectives
To have an understanding of the basic molecular biology and the relevant tools for analyzing
and manipulating at genetic level.
Syllabus
Gene Regulation and Expression in Prokaryotes and Eukaryotes, Vectors for Gene Transfers,
Genetic engineering, Introduction of DNA into living cells, Molecular markers, Introduction to
Gene therapy
Expected outcome
The students will be adept in advanced genetic engineering techniques and the possibilities in
genetic manipulations to treat incurable diseases.
References
1. Principles of Gene manipulation: An introduction to Genetic engineering, Old RW and

Primrose SB
2. Gene Cloning, T.A. Brown.
3. Molecular Cell Biology – Gerald Karp.
4. Genes X, Benjamin Lewin
40
Course Plan
Exam Marks
I Gene Regulation and Expression in Prokaryotes- 15 25%
Lactose, Arabinose and Tryptophan operons,

Repressors and activator, Gene regulation in Eukaryotic
system, Repetitive DNA, Promoters, enhancer elements,
gene amplification. Transposons, Applications of
transposons, Retrotransposons.
First Internal Exam
II Vectors for Gene Transfers.Purification of genomic 14 25%
DNA from living cells, Manipulation of purified DNA;

Construction of prototype vector (pBR 322), Different
types of cloning vectors (plasmid – pUC 19, λ phage,
Cosmid, M13, Phasmid, Phagemid, YAC and BAC).
Enzymes involved in genetic engineering; cloning
strategies.
.
41
III Introduction of DNA into living cells. Methods of Gene 15 25%
transfer, Restriction mapping. Expression of cloned

genes in yeast &E.coli. Blot analysis - Southern,
Northern & Western blot; dot and slot blot. Genomic
and cDNA library construction and application.DNA
sequencing. Molecular markers: RFLP, RAPD, AFLP,
16S rRNA typing, gene chip and micro array;
applications in disease profile.
IV Introduction to Gene therapy (Ex vivo &In vivo), case 15 25%
study of ADA as an example. Advantages and

limitations of Gene therapy. Basic molecular
mechanism of gene transfer, prerequisite of human
gene therapy, biological basis of gene therapy
strategies, vehicles for gene transfer, clinical gene
therapy studies, gene therapy for hereditary disease,
gene therapy for cancer, gene therapy for HIV.
End semester Exam
42
Course Course Name L-T-P- Year of
No. Credits Introduction
03 BH BIOREACTION ENGINEERING 3-0-0-3 2015

6022
Course Objectives
To understand the reaction mechanism of cell, various types of reactions and how it affects mass transfer effects.
Syllabus
Reaction kinetics, Homogenous Reactions; General reaction Kinetics for biological system, Interpretation of batch reactor,
Heterogeneous Reactions, Ideal reactors, Analysis of non- ideal behavior in bioreactors.
Expected outcome
The aims of the course are to review fundamentals and provide an up-to-date account of current knowledge in reaction
engineering.
References
1. Chemical Reaction Engineering: Octave Levenspiel

2. Bioreaction Engineering, K. Schergeri, Vols 1 & 2, John Wiley. 1985.
3. S.M. Walas, “Reaction Kinetics for Chemical Engineers”, McGraw Hill, New York.
4. Elements of Chemical Reaction Engineering: H.Scott, Fogler.
5. Bioreaction engineering- Principle Nilesons S. and Villadsen J
6. Basic Biotechnology, edited by Colin Ratledge and Bjorn Kristiansen, Cambridge
University Press 2003.
7. Biochemical Engineering Fundamentals, Bailey, and Ollis, McGraw Hill Book Co.1986.
Course Plan
43
Exam
Marks
I Reaction kinetics: - Reaction thermodynamics, order and 16 25%

molecularity of reaction, homogeneous and heterogeneous
reactions, elementary and non elementary reactions, reaction
yield, reaction rate, calculation of reaction rates from
experimental data, general reaction kinetics for biological
system, production kinetics in cell culture, kinetics of substrate
uptake in cell culture, growth kinetics with plasmid instability,
kinetics of bi-substrate enzyme reactions, kinetics of enzyme
deactivation.
.
First Internal Exam
II Homogenous Reactions: - General reaction Kinetics for 16 25%

biological system: Zero –order kinetics, First-order kinetics,
Michaelis-Menten Kinetics, effect of conditions on enzyme
reaction rate.
Determining enzyme kinetic constant from batch data:

Michaelis-Menten plot, Lineweaver Burk plot, Eadie-Hofstee
plot. Langmuir plot, Direct linear plot. Numerical Problems.
III Interpretation of batch reactor: Constant volume batch reactor, 15 25%

integral method of analysis of data, series and parallel
reactions, reversible reactions- concept of equilibrium
conversion. Variable volume batch reactor, development of
rate equations for different homogeneous reactions. Collection
and analysis of batch reactor data: differential method of rate
analysis, integral method, method of half lives, Heterogeneous
Reactions.
44
IV Ideal reactors: Isothermal batch, mixed flow reactor, plug flow 15 25%
reactor, semi-batch reactors; concept of holding and space
time. Performance equations for single reactors; comparison of
productivity in plug flow and CSTR. Numerical problems.
Analysis of non- ideal behavior in bioreactors- reasons for non

ideality-importance
Course No. of Name
Course RTD studies- stimulus-response
L-T-P-Credits Year of
experiment-circulation time distribution, exit age distribution, Introduction
F-curve and C-curve- mean and variance of residence time-
diagnosis ofAPPLIED
03 BH 6032 ills of flow reactors. Numerical problems.
BIOINFORMATICS 3-0-0-3 2015
End semester Exam
45
Course Objectives
This course is formulated to provide students an in depth knowledge of biological data analysis using compilation methods. It is
also useful for investigating molecular biology problems from computational perspective.
Syllabus
Sequence-alignment related problems, Pattern analysis in sequences, Structure-related problems, Molecular dynamics, and
System-wide analyses.
Expected outcome
At the end students gain expertise with existing tools and resources for computational analysis of biological data. They develop
an understanding of problems related to genomics and proteomics, which will be useful in the modeling and analysis of living
system
References
1. David W. Mount, Bioinformatics: Sequence and Genome Analysis 2nd Edition, CSHL Press, 2004.
2. A. Baxevanis and F. B. F. Ouellette, Bioinformatics: a practical guide to the analysis of genes and proteins, 2 nd Edition,
John Wiley, 2001.
3. Jonathan Pevsner, Bioinformatics and Functional Genomics, 1 st Edition, Wiley-Liss, 2003.
4. P. E. Bourne and H. Weissig, Structural Bioinformatics. Wiley, 2003.
5. C. Branden and J. Tooze, Introduction to Protein Structure, 2 nd Edition, Garland Publishing, 1999.
Course Plan

Exam
Marks
I Sequence-alignment related problems: Sequence 16 25%

databases; Similarity matrices; Pairwise alignment;
BLAST; Statistical significance of alignment; Sequence
assembly; Multiple sequence alignment; Clustal;
Phylogenetics: distance based approaches, maximum
parsimony
46
First Internal Exam
II Pattern analysis in sequences: Motif representation: 16 25%

consensus, regular expressions; PSSMs; Markov
models; Regulatory sequence identification using
Meme; Gene finding: composition based finding,
sequence motif-based finding.
III Structure-related problems: Representation of molecular 15 25%

structures (DNA, mRN A, protein), secondary
structures, domains and motifs; Structure classification
(SCOP, CATH); Visualization software (Pymol,
Rasmol etc.); Structure databases; Secondary structure
prediction; RNA structure prediction; Mfold; Protein
structure prediction by comparative modelling
approaches (homology modelling, threading); Ab initio
structure prediction: force fields, backbone conformer
generation by Monte Carlo approaches, side-chain
packing; Energy minimization; Molecular dynamics;
Rosetta; Structure comparison (DALI, VAST etc.);
CASP; Protein-ligand docking; Computer-aided drug
design (pharmacophore identification); QSAR; Protein-
Protein interactions
47
IV System-wide analyses: Transcriptomics: Microarray 15 25%
technology, expression profiles, data analysis; SAGE;
Proteomics: 2D gel electrophoresis; Mass Spectrometry;
Introduction
Protein arrays; Metabolomics: 13C NMR based
metabolic flux analysis.
03 BH 6042 ENZYME ENGINEERING AND 3-0-0-3 2015
TECHNOLOGY
End semester Exam
48
Course Objectives
This course is designed to provide students with fundamental knowledge of optimization,

modeling and design of enzymatic process.
Syllabus
Enzymes, Mechanisms and Kinetics of Enzyme Action, Enzyme Inhibition, Enzyme
Immobilization, Overview of application of immobilized enzyme systems, Mass Transfer
Effects In Immobilized Enzyme Systems, Design of Enzyme Reactors Analysis of Film and
Pore Diffusion Effects, Enzyme Reactors, Isolation of Enzymes, Enzyme Biosensors.
Expected outcome
Students will be able to implement fundamental and emerging knowledge to design new and
important enzymatic processes.
References
 Enzymes by Trevor palmer , East west Press

 Biochemical engineering fundamentals, second edition. James E Bailey, David F., Ollis,
Mc Graw Hill Intl. Edition.
 Wiseman, A: Handbook of Enzyme Biotechnlogy, 3rd Edition, Ellis Horwood
Publication.
 Biochemical Engineering Principles and functions by Syed Trnveer Ahmed Inamdar, PHI
Learning Private limited.
Course Plan

Exam
Marks
49
I Enzymes- Nature, Classification ; Purification and 16 25%
characterization of enzymes from natural sources
Comparison of chemical and enzyme catalysis.
Mechanisms and Kinetics of Enzyme
Action:Mechanisms of Enzyme Action; Concept of
active site and energetics of enzyme substrate complex
formation; Specificity of enzyme action; Kinetics of
single substrate reactions; turnover number; estimation
of Michaelis-Menton parameters. Importance of Km.
Multi-substrate reaction mechanisms and kinetics.
First Internal Exam
II Enzyme Inhibition:Types of Inhibition- kinetic models; 16 25%

Substrate and Product Inhibition; Allosteric regulation
of enzymes; Deactivation kinetics
Enzyme Immobilization: Physical and Chemical

techniques for enzyme Immobilization - adsorption.
Matrix entrapment, encapsulation. cross-linking.
covalent binding - examples; Advantages and
disadvantages of different Immobilization techniques.
Overview of application of immobilized enzyme

systems.
III Mass Transfer Effects In Immobilized Enzyme Systems 15 25%

and Design of Enzyme Reactors, Analysis of Film and
Pore Diffusion Effects on kinetics of Immobilized
Enzyme Reactions; Formulation of dimensionless
groups and calculation of Effectiveness Factor
Enzyme Reactors: Design of Immobilized Enzyme

Reactors-Packed- bed, Fluidized-bed Membrane
reactors; Bioconversion calculations in free- enzyme
CSTRs and immobilized enzyme reactors.
50
IV Isolation of Enzymes:Extraction and Purification of 15 25%

Crude Enzyme extracts from plant, animal and
microbial sources-some case studies; methods of
characterization of enzymes; development of enzymatic
assays.
Enzyme Biosensors: Different types.Applications of

enzymes in analysis; Design of enzyme electrodes and
their applications as biosensors in industry, health care
and environment.
End semester Exam
51
Introduction
03 BH 6052 BIOTECHNOLOGY RESOURCE 3-0-0-3 2015

PLANNING, IPR AND BIOSAFETY
Course Objectives
This course is designed to provide students with fundamental knowledge of Resource base for process
biotechnology, Types of Patents, Guidelines for Biosafety and Biotechnology Related Issues.
Syllabus
Economic, social and product benefits of modern biotechnology, IPR, Basics of Patents, Biosafety and Risk
analysis.
Expected outcome
Students will be able to plan resources for biotechnology processes, identify IPR prospects as well as suggest
biosafety procedure proportionate to the risks involved.
References
1. BAREACT, Indian Patent Act 1970 Acts & Rules, Universal Law Publishing Co. Pvt. Ltd., 2007
2. Kankanala C., Genetic Patent Law & Strategy, 1st Edition, Manupatra, Information Solution Pvt. Ltd.,
2007
3. S.S.Kanka Entrepreneurship Development, S.Chand and Co, New Delhi 1997
52
Course Plan

Exam
Marks
I Economic, social and product benefits of modern 16 25%

biotechnology; Resource base for process
biotechnology; Typical stages in commercialization of
process/ product; Commercial and financial aspects of
bioprocessing; Financial appraisal of bioprocessing
projects.
First Internal Exam
II IPR: Types of IP- Patents, Trademarks, Copyright & 16 25%

Related Rights, Industrial Design, Traditional
Knowledge,Geographical Indications, Protection of
New GMOs; International framework for the protection
of IP; IP as a factor in R&D; IPR issues in relation to
biotech products/ processes; Architecture of Patent
application. Alternative models of technology transfer
and licensing; Good manufacturing practices; Funding
mechanisms of commercial projects.
53
III Basics of Patents: Invention in context of “prior art”; 15 25%
Patent databases; Types of patents; Indian Patent Act
1970; Recent Amendments; Filing of a patent
application; Precautionsbefore patenting-
disclosure/non-disclosure; WIPO Treaties; Budapest
Treaty; PCT and Implications; Role ofa Country Patent
Office; Procedure for filing a PCT application
IV Biosafety - Introduction; Historical Backround; 15 25%

Introduction to Biological Safety Cabinets; Primary
Containment for Biohazards; Biosafety Levels;
Biosafety Levels of Specific Microorganisms;
Recommended Biosafety Levels for Infectious Agents
and Infected Animals; Biosafety guidelines -
Government of India; Definition of GMOs & LMOs;
Roles of Institutional Biosafety Committee, RCGM,
GEAC etc. for GMO applications in food and
agriculture; Environmental release of GMOs; Risk
Analysis; Risk Assessment; Risk management and
communication; Biotechnology Related Issues of Public
Concern - Bioethics
End semester Exam
54
Introduction
03 BH 6062 BIOLOGICAL TREATMENT OF WASTE 3-0-0-3 2015
Course Objectives
The purpose of this course is to provide specialized knowledge in the area of waste water treatment processes. The
course will provide fundamental principles of aerobic and anaerobic biological waste treatment processes,
application of microbial systems to the operations and design of waste (domestic, industrial) treatment processes
Syllabus
Activated Sludge Process, Aerobic Fixed-Film and Anaerobic Treatment Processes, Advanced Waste Water
Treatment, Environmental Concerns and Recycling of Wastes.
Expected outcome
At the end of the course, students will develop knowledge and skills to know the nature of raw waste water,
treatment objectives, number and sequence of unit processes, the fundamental and scientific basis governing the
design and performance of the treatment technologies.
References
1. Wastewater Engineering: Treatment Disposal Reuse by Metcalf & Eddy
2. Environmental Biotechnology : Principles and Applications by Bruce E. Rittmann
3. Waste water Engineering Treatment and Reuse: McGraw Hill, G. Tchobanoglous, FI Biston,
2002.
4. Industrial Waste Water Managemnet Treatment and Disposal by Waste Water McGraw Hill,
3rd Edition 2008.
5. Environmental Biotechnology: Principles and Applications by Bruce E. Rittmann.
6. Biological Wastewater Treatment, Second Edition, Marcel Dekker, Inc., New York.
55
Course Plan

Exam
Marks
I Activated Sludge Process-Process Analysis and 16 25%

Selection: Characteristics of Activated Sludge (aerobic
and anaerobic); Analysis of Data – Mass Balance
Analysis. Reactors used in waste water treatment- Up
Flow Anaerobic Sludge Blanket (UASB), Two-stage
Aerobic UNI Tank System (TSU-System), Route Zone
Treatment, Submerged Aerobic Fixed Film (SAFF)
Reactor, and Fluidized Aerobic Bioreactor (FAB).
First Internal Exam
II Aerobic Fixed-Film and Anaerobic Treatment 16 25%

Processes: Biofilm process considerations; Trickling
Filters and Biological Towers; Rotating Biological
Contactors; Granular – Media Filters; Fluidized – Bed
and Circulating Bed- Biofilm reactors. Hybrid
Biofilm/suspended growth processes. Anaerobic
Processes: Methanogenesis, process chemistry and
microbiology; process kinetics and factors for the
design of anaerobic digestors
56
III Advanced Waste Water Treatment: Technologies used 15 25%
in advanced treatment – Classification of technologies;
Removal of Colloids and suspended particles – Depth
Filtration – Surface Filtration – Membrane Filtration
Absorption – Ion Exchange – Advanced oxidation
process - Activated Carbon, Air Stripping, Heavy
Metals Removal, Steam Stripping, Chemical
Precipitation, and Electrolysis.
IV Environmental Concerns and Recycling of Wastes: 15 25%

Environmental regulations and technology- Regulatory
Concerns, Technology; Laws, regulations and permits-
Air, Water, Solid Waste, Environmental Auditing,
National Environmental Policy act, Occupational Safety
and Health Act (OSHA), Storm Water Regulations;
Technology (waste water); Recycling of Industrial
wastes : paper, plastics, leather and chemicals.
End semester Exam
57
Introduction
03 BH 6072 NANOBIOTECHNOLOGY 3-0-0-3 2015
Course Objectives
The course aims to introduce different nanomaterials and their biomedical applications to students which will be beneficial for
the development of many site directed therapy devoid of side effects.
Syllabus
Introduction to Nanotechnology and Nanomedicine: Properties of nanomaterials, Synthesis of nanomaterials, Tools for the
characterization of nanomaterials. Types of nanomaterials and their biomedical applications: nanoparticles for molecular
diagnostics, Nanobiosensors. Nanopharmaceuticals: drug delivery by using different types of nanoparticles, Nanoparticle based
therapies, Fluorescent Nanoparticles. Nanostructures for Tissue Engineering/Regenerative Medicine. Ethical, Safety, and
Regulatory Issues of Nanomedicine.
Expected outcome
At the end of the course the student will be able to understand promises and purpose of nanotechnologies and also helps to
identify scientific, ethical, social and political issues arising from the development of nanotechnology.
References
 OdedShoseyov and Ilan Levy; Nanobiotechnology: Bioinspired Devices and Materials of the Future,
Humana Press; 1 edition (2007)
 M. Reza Mozafari; Nanomaterials and Nanosystems for Biomedical Applications, Springer; 1 edition
(2007)

 Kewal K. Jain; The Handbook of Nanomedicine, Humana Press
 Elisabeth S. Papazoglou, Aravind Parthasarathy; Bio Nanotechnology, Morgan & Claypool Publishers.
 Kenneth E. Gonsalves, Craig R. Halberstadt, Cato T. Laurencin, Lakshmi S. Nair; Biomedical
Nanostructures, Wiley & Sons Inc.
 Rolando Barbucci; Integrated Biomaterials Science, Springer
 Vijay K. Varadan, Linfing Chen, JiningXie; Nanomedicine: Design and Applications of Magnetic
Nanomaterials, Nanosensors and Nanosystems, Wiley.
58
Course Plan

Exam
Marks
I Introduction to Nanotechnology and Nanomedicine: 16 25%

Visualization and Manipulation on
Nanoscale.Unique properties of nanomaterials;
magnetic, electrical, thermal and mechanical
properties. Approaches for the synthesis of
nanomaterials; bottom up approaches and top-down
approaches, tools for the characterization of
nanomaterials; Atomic Force Microscopy (AFM),
Magnetic Resonance Force Microscopy, Scanning
Probe Microscopy, Scanning Electron Microscopy,
TEM, scanning tunneling microscopy (STM), XRD.
First Internal Exam
II Different types of nanomaterials and their biomedical 16 25%

applications:Nanomolecular diagnostics; nanoparticles
for molecular diagnostics, gold nanoparticles, quantum
dots, magnetic nanoparticles,
nanobarcodetechnology.Nanobiosensors; cantilevers as
biosensors for molecular diagnosis, carbon nanotubes,
FRET based DNA nanosensors, viral nanosensors,
PEBBLE nanosensors, optical nanosensors.
59
III Nanopharmaceuticals; drug delivery by using gold 15 25%
nanoparticles, QDs, dendrimers, fullerenes, liposomes,
nanoshells, targeted drug delivery using nanoparticles.
Nanoparticle based therapies; stem cell therapy, gene
therapy, nanomachines for gene delivery, antisense
therapy, RNA interference; nanoparticle SiRNA
delivery, nanodevices for medicine and surgery.
Fluorescent Nanoparticles, Bacterial Structures
Relevant to Nanobiotechnology, Cubosomes,
Dendrimers, DNA–Nanoparticle Conjugates, DNA
Octahedron, Fullerenes, Nanoshells, CarbonNanotubes ,
Nanopores, Nanostructured Silicon and Molecular
Motors.
IV Nanostructures for Tissue Engineering/Regenerative 15 25%

Medicine: nanobiomaterials for tissue engineering,
replacement of diseased tissue, cell culture and in vitro
tissue development, development of skin. Ethical,
Safety, and Regulatory Issues of Nanomedicine;
biocompatibility of nanomaterials.
End semester Exam
60
Introduction
03 BH 6082 PLANT BIOTECHNOLOGY 3-0-0-3 2015
Course Objectives
The course will provide an overview of plant biotechnology with focus on industrial applications. Also it provides basic
knowledge in plant biology, plant molecular biology and plant biochemistry.
Syllabus
Introduction to cell and tissue culture, Tissue culture media, Embryo culture and embryo rescue, Protoplast isolation,
culture and fusion, Cryopreservation, DNA banking for germ plasm conservation, Plant Transformation technology,
mechanisms of DNA transfer, viral vectors and their applications, Transgene stability and gene silencing, Biopesticides and
Bioinsecticides, Phytoremediation, Single Cell Proteins.
Expected outcome
Upon completion of the course, the student will be able to describe the plant cell, its characteristic organelles as well as
the composition, structure and properties of the plant cell wall and different methods for transformation of plants or plant
cells, including their specific advantages and applications.
References
 Hammond J, McGarvey P and Yusibov V (Eds), Plant Biotechnology, Springer Verlag,

 Fu. G. Singh T.J and Curtis W.R. (Eds), Plant Cell and Tissue Culture for the production of Food Ingredients, Kluwer
Academics/Plenum Press, 1999.
 Henry R.J, Practical Application of Plant Molecular Biology, Chapman and Hall, 1997.
 Gupta P.K, Elements of Biotechnology, Rastogi and C. Meerut, 1996
 Arie Altman, Agricultural Biotechnology, Marcel Dekker, Inc, 2001.
 Neal Stewart, Jr. C, Plant Biotechnology and Genetics: Principles, Techniques and Applications, John Wiley, 2008.
 Dutta Gupta S and Yasuomi Ibaraki, Plant Tissue Culture and Engineering, Springer, 2006.

61
Course Plan

Exam
Marks
I Introduction to cell and tissue culture, tissue culture as a 16 25%

technique to produce novel plants and hybrids-Tissue
culture media (composition and preparation), Initiation
and maintenance of callus and suspension culture,
production of virus-fee plants- Embryo culture and
embryo rescue.
First Internal Exam
II Protoplast isolation, culture and fusion : selection of 16 25%

hybrid cells and regeneration of hybrid plant.
Symmetric and asymmetric hybrids. Cybrids. Anther,
pollen and ovary culture for production of haploid
plants and homozygous lines. Cryopreservation, slow
growth and DNA banking for germ plasm conservation.
III Plant Transformation technology : basis of tumour 15 25%

formation, hairy root , features of Ti and Ri plasmids-
mechanisms of DNA transfer , role of virulence genes,
use of Ti and Ri as vectors, binary vectors . Use of 35S
and other promoters, genetic markers, reporter genes,
use of scaffold. Methods of nuclear transformation ,
viral vectors and their applications-Multiple gene
transfers. Vectors less or direct DNA transfer, particle
bombardment, electroporation, microinjection.
Transgene stability and gene silencing.
62
IV Biopesticides and Bioinsecticides– Biological Sources, 15 25%

Collection, Characters & Constituents. Biocontrol
Methods, Engineered Biocontrol Methods. male sterile
lines, bar and barnase systems. Biofertilizers &
Vermiculture , Phytoremediation , Medicinal Plants &
Nutraceuticals , Floriculture , Biofuels, Single Cell
Proteins.
End semester Exam
63
Introduction
03 BH 6802 BIOTECHNOLOGY AND 0-0-2-1 2015

BIOCHEMICAL ENGINEERING
LABORATORY-II
Course Objectives
The lab integrates practical exposure to molecular biology experiments with the ability to
analyze data with computational tools, which will equip them to handle complex experiments
and arrive at fair results.
Syllabus
Molecular biology and genetic engineering experiments, animal cell culture experiments,
bioinformatics tools, biochemical experiments.
Expected outcome
Students will prepare, perform, observe and analyze results of biochemical experiments
independently.
References
1. Current Protocols in Molecular biology. 2003. Frederick M Ausubel, Roger Brent, Robert E
Kingston erat.John Wiley & sons Inc.
2. Molecular Cloning: A Laboratory Manual, JosephSambrook and David W. Russell, Cold

spring harbor Laboratory press.
3.Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, R. Ian
Freshney
64
LIST OF EXPERIMENTS
MOLECULAR BIOLOGY AND GENETIC ENGINEERING EXPERIMENTS
1. Isolation of Genomic DNA, visualization and quantification.
2. Restriction mapping.
3. Competent cell preparation, Transformation and screening for recombinants (α-

complementation study).
4. SDS PAGE for protein separation, silver staining and Western blotting.
5. PCR
6. RFLP
ANIMAL CELL CULTURE EXPERIMENTS
7. Sterilization procedures in animal cell culture
8. Preparation of media
9. Subculturing and maintenance of cell lines
10. Cytotoxicity evaluation by Trypan blue staining
BIOINFORMATICS TOOLS
11. Homology Search tools including BLAST, CD search, Homologene, Protein Clusters,
Genome BLAST
12. NCBI tools : OMIM- Online Mendelian inheritance in man, Unigene- computational analysis
of expression, MapViewer- provide a variety of genome mapping and sequencing data, ORF
Finder: To find out ORF regions in a given sequence, Primer BLAST-To design PCR primer
pairs, VecScreen- To quickly identify sequences that maybe of vector origin, mfold- predict
secondary structure of RNA, Swiss PDB viewer, Rasmol- protein structure visualization tools,
CN3D, Chempen3D- drug structure drawing tools
65
BIOCHEMICAL EXPERIMENTS
13. RTD studies in CSTR
14. RTD studies in PFR
(Minimum of 12 experiments should be performed in the Laboratory)
Marks: Continuous evaluation: 100
i) Practical Records /outputs 40%
ii) Regular Class Viva-Voce 20%
iii) Final Test (Objective) 40%
66
Introduction
03 BH 6902 MINIPROJECT 0-0-4-2 2015
Marks:
Miniproject Report Evaluation: 50 Marks

Miniprojecct Presentation and evaluation by the committee: 50 Marks
67
SEMESTER III
68
Introduction
03 BH 7003 ADVANCED FERMENTATION 3-0-0-3 2015

TECHNOLOGY
Course Objectives
To understand the mechanism of cell growth kinetics, metabolism, scale up of processes in industry and physic-chemical
parameters that determine production.
Syllabus
Introduction to fermentation processes, Design equations for ideal reactors, Sterilization reactors- batch heat sterilization of
liquids and continuous heat sterilization of liquids, Convective mass transfer.
Expected outcome
The aims of the course are to review fundamentals and provide an up-to-date account of current knowledge in biological and
biochemical technology. The lectures will emphasize and place perspectives on biological systems with industrial practices.
References
1. James E. Bailey and David F. Olis, Biochemical Engineering Fundamentals (2nd Edition), Mc
Graw Hill International Series
2. Paulin M Doran, Bioprocess Engineering Principles - Academy Press
3.Michael L shuler, Fikertkargi, Bioprocess Engineering, peason education international services
4. Stanburry P F, Stefan J Hall and A Whitaker, Principles of Fermentation Technology
5. Muo – Young ,Comprehensive Biotechnology –(ed) Pergamon Press
6.Wang.D.I.C Cooney C.L., Demain A.L., Dunnil.P. Humphrey A.E. Lilly M.D, Fermentation and Enzyme Technology John
Wiley and sons 1980.
7. Stanbury P.F., and Whitaker A., Principles of Fermentation Technology, Pergamon Press, 1984.
69
Course Plan

Exam
Marks
I Introduction to fermentation processes- classification – 16 25%

aerobic and anaerobic fermentation, submerged and
solid state fermentation - Cell kinetics-batch growth,
balanced growth-effect of substrate concentration-
Monod equation.-growth kinetics with plasmid
instability-production kinetics-structured and
unstructured models.
First Internal Exam
II Design equations for ideal reactors-batch fermenter, 16 25%

fed-batch fermenter, continuous operations of mixed
reactor, chemostat with immobilized cells, chemostat
cascade, chemostat with cell recycle, continuous
operation of plug flow reactor, comparison between
major modes of reactor operations.
III Sterilization reactors- batch heat sterilization of liquids 15 25%

and continuous heat sterilization of liquids.
Immobilized cell bioreactor, packed bed, bubble
column reactors, fluidized bed bioreactor, trickle bed
reactor, Selection criteria for fermentation air filters-air
sterilization, principle and design-scale-up of stirred
tank fermenters
70
IV Convective mass transfer, gas- liquid mass transfer- 15 25%

rates of metabolic oxygen utilization-oxygen transfer in
fermenters, determination of oxygen transfer rates,
measurement of KLa, factors affecting KLa, estimation
of power requirement for sparged and agitated vessels.
Chemical and physical factors affecting mass transfer.
End semester Exam
71
Introduction
03 BH 7013 2015
MODELLING AND SIMULATION OF
BIOPROCESSES 3-0-0-3
Course Objectives
To understand the fundamentals and basic modeling techniques in different systems.
Syllabus
Basic modeling principles, Mathematical models for chemical engineering systems, modeling of different reactors, Digital
simulation, Numerical integration.
Expected outcome
This subject will lead to the analysis and understanding of observed phenomena and testing of hypotheses and theories.
References
 Luyben W.L., Process Modelling Simulation and Control for Chemical Engineers, McGraw Hill.
 Franks R.G.E., Mathematical Modelling in Chemical Engineering, John Wiley
 JohnInghamet.al.,Chemical Engineering Dynamics- Modelling with PC Simulation,VCH

Publishers.
 BiquetteW.B.,Process Dynamics- Modelling Analysis and Simulation, Prentice Hall.
72
Course Plan

Exam
Marks
I Basic modeling principles – uses of mathematical modelling- 16 25%

classification of modelling techniques- fundamental laws-
energy equations – continuity equation – equations of motion-
transport equations- equations of state- equilibrium states and
chemical kinetics– examples.
First Internal Exam
II Mathematical models for chemical engineering systems – 16 25%

continuous flow tanks-enclosed – enclosed vessel- mixing vessel-
mixing vessel- mixing with reaction-reversible reaction – steam
jacketed vessel- boiling of single component liquid – open and
closed vessel – continuous boiling system- batch distillation.
73
III Gas flow systems – hydraulic transients between two reservoirs- 15 25%
reaction kinetics-general modeling scheme-liquid phase CSTR-
batch reactor-distributed systems-jacketed tubular reactor-CSTR
in series- non isothermal CSTR.
IV Ideal binary distillation column--laminar flow in a pipe-counter 15 25%

current heat exchanger. Digital simulation-numerical integration-
Euler and fourth order RungeKutta methods-simulation of gravity
flow tank-- binary distillation column-batch reactor.
End semester Exam
74
Introduction
03 BH 7023 2015
METABOLIC ENGINEERING 3-0-0-3
Course Objectives
The overall objective of this course is to provide a quantitative basis, based on thermodynamics, enzyme
kinetics, metabolic flux analysis and metabolic control analysis, for the understanding of metabolic
networks in single cells and at the organ level.
Syllabus
Introduction to Metabolic Engineering: Basic concepts of Metabolic Engineering, Synthesis of Primary
and Secondary Metabolites, Regulation of Enzyme Production. Bioconversions. Applications of
Metabolic Engineering.
Expected outcome
At the end the student will be able to apply the knowledge of science and engineering to integrate modern
biology with engineering principles. Also they can identify, formulate, and solve engineering problems.
References
 Wang.D.I.C Cooney C.L., Demain A.L., Dunnil.P. Humphrey A.E. Lilly M.D.,
Fermentation and Enzyme Technology, John Wiley and sons, 1980.
 Stanbury P.F., and Whitaker A., Principles of Fermentation Technology,
Pergamon Press, 1984.
 Wendisch, Volker F. (Ed.) Amino Acid Biosynthesis – Pathways, Regulation and Metabolic
Engineering, Microbiology Monographs, 2007
75
Course Plan

Exam
Marks
I Introduction to Metabolic Engineering-Identification of 16 25%

metabolic regulation is a key point in metabolic
engineering. Basic concepts of Metabolic Engineering –
Overview of cellular metabolism – Different models for
cellular reactions.
First Internal Exam
II Synthesis of Primary and Secondary Metabolites- 16 25%

Amino acid synthesis pathways and its regulation at
enzyme level and whole cell level, Alteration of
feedback regulation, Limiting accumulation of
endproducts. Regulation of secondary metabolite
pathways, precursor effects, prophase, idiophase
relationship, producers of secondary metabolites.
III Regulation of Enzyme Production-Strain selection, 15 25%

Genetic improvement of strains, Gene dosage,
metabolic pathway manipulations to improve
fermentation, Feedback repression, Catabolite
Repression, optimization and control of metabolic
activities. The modification of existing-or the
introduction of entirely new -metabolic pathways.
76
IV Bioconversions-Applications of Bioconversions, 15 25%

Factors affecting bioconversions, Specificity,
Yields,Co-metabolism, Product inhibition, mixed or
sequential bioconversions, Conversion
of insoluble substances.
Applications of Metabolic Engineering. Application in
pharmaceuticals, chemical bioprocess, food technology,
agriculture, environmental bioremediation and biomass
conversion.
End semester Exam
77
2015
FOOD BIOTECHNOLOGY 3-0-0-3
03 BH 7033
Course Objectives
The objective of the course is to familiarize the students with advanced research area and basic concept in Food
Biotechnology.
Syllabus
Biotechnology relating to the food industry, Technological aspects of industrial production. Fermented Foods,
Application of enzymes in food industry. Application of genetics to food production. Genetically modified
foods (GMF),Food adulteration and food safety . Principles of Preservation methods. Food labeling, Food
allergy , Food intolerance , Sanitation..
Expected outcome
At the end of the course, the students will have sufficient scientific understanding of different types of
biotechnological methods to improve the value of different food and new techniques used in Food
Biotechnology.
References
 Stanbury, P.F., Allan Whitaker and S.J. Hall. “Principles of Fermentation Technology” Aditya books
private Ltd., New Delhi. 1997
 Roger A., Gorden B., and John ., “ Food biotechnology”, 1989
 Jelen, P. 1985. Introduction to Food Processing. Prentice Hall, Reston Virginia, USA.
 Inteaz Alli, “Food Quality assurance-Principles and Practices”, CRC Press, 2004.
 Mehta R. and George J., “Food Safety Regulation Concerns And Trade- The Developing
Country Perspective”, Macmillan India Ltd., New Delhi. 2005.
 Knorr, D. 1982. Food Biotechnology. Marcel Dekker, New York
78
Course Plan

Exam Marks
I Introduction -Biotechnology relating to the food 16 25%

industry – role of bioprocess engineering in
biotechnology industry. Regulatory and Social
aspects of biotechnology in foods. Technological
aspects of industrial production of beer and
wine, bakers yeast, vitamins, single cell protein,
food flavor – food color – food enzymes.
production of food flavour, colour, enzymes,
Immobilised enzymes .
Fermented Foods: Scope and development,

benefits of fermentation, sauerkrauts,
yoghurt.Application of enzymes in food
industry,.
First Internal Exam
II Application of genetics to food production. 16 25%

Methods of molecular cloning, immobilization
of microbial and cultured plant cells. Plant tissue
culture. Genetically modified foods (GMF).Food
adulteration and food safety.
79
III Principles of Preservation methods, fermentation 15 25%
methods for preservation, and chemical
preservations of foods. Food preservation by
low-temp: Refrigeration, freezing and freeze-
drying. Food preservation by heating: drying,
osmotic dehydration, blanching, canning,
pasteurization, sterilization, extrusion cooking.
Non-thermal preservation: Hydrostatic pressure,
dielectric heating, microwave processing, hurdle
technology, membrane technology, irradiation.
IV Food labeling - Food allergy - Food intolerance - 15 25%

Sanitation - Need for sanitation- safe handling –
cross contamination -cleaning and cleaners- CIP
cleaning sanitizers, hygiene and safety rules for
building and equipments – cleaner production in
food industry – fruit and vegetable processing -
sea food processing - brewing and wine
processing
End semester Exam
80
Introduction
03 BH 7043 BIOENERGY AND BIOFUEL 2015

TECHNOLOGY 3-0-0-3
Course Objectives
To understand the mechanism of bioenergy and production of bio-fuels so as to make it as an alternate fuel for
upcoming depleting energy sources.
Syllabus
Biomass Sources, Characteristics and Preparation. Pyrolysis and Gasification of Biomass. Clean Coal Technology.
Biogas Technology, Microbial and biochemical aspects, Digesters for rural application. Microbial Fuel Cell: types,
working principle and applications. Combustion of Biomass and Cogeneration Systems. Biofuels: orgin, chemical
and physical properties, advantage and disadvantage. Biorefinery concept.
Expected outcome
The lectures will emphasize and place perspectives on biological systems with industrial practices for bioenergy and
bio-fuel technology.
References
 Chakravarthy A, Biotechnology and Alternative Technologies for Utilization of Biomass or

AgriculturalWastes, Oxford and IBH publishing Co, 1989.
 Mital K.M, Biogas Systems: Principles and Applications, New Age International publishers (P) Ltd.,
1964.
 Nijaguna, B.T, Biogas Technology, New Age International publishers (P) Ltd., 2002.
 Venkata Ramana P and Srinivas S.N, Biomass Energy Systems, Tata Energy Research
Institute, 1996.
 Rezaiyan. J and N. P. Cheremisinoff, Gasification Technologies, A Primer for Engineers and Scientists,
Taylor and Francis, 2005.
81
Course Plan

Exam
Marks
I Biomass Sources, Characteristics and Preparation: 16 25%

Biomass Sources and classification. - Chemical
composition and properties of different biomass
materials Energy plantations -Preparation of woody
biomass: Size reduction, Briquetting of loose biomass,
Drying, Storage and Handling of Biomass.
Pyrolysis and Gasification of Biomass: Thermo-

chemical conversion of ligno- cellulose biomass,
Biomass processing for liquid fuel production -
Pyrolysis of biomass – Pyrolysis regime, effect of
particle size, temperature and products obtained.
Thermo-chemical gasification principles: Effect of
pressure, temperature and of introducing steam and
oxygen.
Clean Coal Technology: Biotechnology and
Microbiology of Coal Degradation – Aerobic and
coal degradation- Biosolubilization and bioliquefaction
of coal- Biodesulfurisation of coal and oil- Mechanisms
of coal biosolubilization- Enzymes that depolymerise
coal – Recent Advances in Bioprocessing of coal.
First Internal Exam
82
II Biogas, Technology: Feedstock for biogas production, 16 25%
Aqueous wastes containing biodegradable organic matter,
animal residues. Microbial and biochemical aspects-
Operating parameters for biogas production. Kinetics and
mechanism - Dry and wet fermentation. Digesters for rural
application - High rate digesters for industrial waste water
treatment. KVIC plants, process kinetics, digester design,
sludge treatment, energy from wastes – development in
energy routes.
III Microbial Fuel Cell: Types of Biological fuel cells – 15 25%

Working Principle - Applications of biological Fuel cells.
A brief study of the principle, construction of different
types of fuel cells. Hydrogen production by photosynthetic
bacteria, biophotolysis of water and by fermentation;
Microbial recovery of petroleum by biopolymers
(Xanthum gum), biosurfactants.
Combustion of Biomass and Cogeneration Systems:

Combustion of Woody Biomass: Theory, Calculations and
Design of Equipments. Cogeneration in Biomass
Processing Industries. Case Studies: Combustion of Rice
Husk, Use of Bagasse for Cogeneration
IV Biofuels: Biodiesel fuels, their origin, chemical and 15 25%

physical properties; Biodiesel production; Advantages and
disadvantages of biodiesel; Storage and use of biodiesel.
Gasohol as a Substitute for Leaded Petrol. - Trans-
Esterification of Oils to Produce Bio-Diesel.Biorefinery
concept – biomass derived chemical products. Policy
issues in biofuels, Indian Biofuel Programme, Bioethanol
: Production of Fuel Ethanol by Fermentation of Sugars.
Biohydrogen technology: potential of organic waste for
hydrogen production; biofuel refining and technology.
End semester Exam
83
03 BH 7053 ANIMAL CELL TECHNOLOGY 3-0-0-3 2015
Course Objectives
Understanding the fundamental principles of cell science and its application in advanced molecular
biology.
Syllabus
Scope of Animal Biotechnology, Cell culture-Scaling up of animal cell culture, Gene therapy-
prospects and problems, Rumen manipulation .
Expected outcome
At the end of the course, the students will have sufficient scientific understanding of different
techniques in animal cell technology.
References:
1. Watson, J.D., Gilman, M., WitowskiJ.andZoller, M. Recombinant DNA, 2nd ed., Scientific
American Books, 1983
2. Glick, B.R. and Pasternack, J.J. Molecular Biotechnology, 3rd ed., ASM Press, 2003
3. Lewin, B. Genes VIII , Pearson Prentice Hall, 2004
4. Davis J.M. Basic Cell Culture: A Practical Approach, IRL Press, 1998
5. Freshney R.I. Animal Cell Culture- a practical approach, 1987
84
Course plan
Sem.
Module Contents Hours Exam
Marks
I Scope of Animal Biotechnology, Animal 15 25%
Biotechnology for production of regulatory proteins,
blood products, vaccines, hormones and other
therapeutic proteins.Biology of animal viral vectors-
SV40, adeno virus, retrovirus, vaccinia virus, herpes
virus, adeno associated virus and baculo virus.
FIRST INTERNAL EXAM

II Cell culture-Scaling up of animal cell culture- 15 25%
monolayer culture, suspension culture; Various bio-
reactors used for animal cell culture-Roller bottle
culture; Bioreactor process control, stirred animal cell
culture, Air-lift fermentor, Chemostat/Turbidostat;
High technology vaccines; Hybridoma technology; Cell
lines and their applications
III Gene therapy-prospects and problems; Knock out mice 16 25%

and mice model for human genetic disorder; Baculo
virus in biocontrol; Enzymes technology, Somatic
manipulation of DNA, Nucleic acid hybridization and
probes in diagnosis- preparation of probes, evaluation
and applications.
SECOND INTERNAL EXAM

IV Rumen manipulation- probiotics embryo transfer 15
technology, invitro fertilization, transgenesis- 25%
methods of transferring genes into animal oocytes,
eggs, embryos and specific tissues by physical,
chemical and biological methods; Biopharming -
85
Transgenic animals (Mice, Cows, Pigs, Sheep,
Goat, Birds and Insects); Artificial insemination
and embryo transfer
END SEMESTER EXAM
86
Introduction
03 BH 7903 SEMINAR II 0-0-2-2 2015
Students have to register for the seminar and select a topic in consultation with any faculty
member offering courses for the programme. A detailed write-up on the topic of the seminar is to
be prepared in the prescribed format given by the Department. The seminar shall be of 30
minutes duration and a committee with the Head of the department as the chairman and two
faculty members from the department as members shall evaluate the seminar based on the
coverage of the topic, presentation and ability to answer the questions put forward by the
Committee.
Marks:
Seminar Report Evaluation: 50 Marks
Seminar Presentation and evaluation by the committee: 50 Marks
87
Introduction
03 BH 7913 PROJECT PHASE -I 0-0-8-6 2015
Project Progress evaluation:
Progress evaluation by the Project Supervisor : 20 Marks
Presentation and evaluation by the committee : 30 Marks
88
SEMESTER IV
89
Introduction
03 BH 7914 PROJECT PHASE -II 0-0-21-12 2015
The student has to continue the project work done third semester. There would be an interim
presentation at the first half of the semester to evaluate the progress of the work and at the end of
the semester there would be a pre-Submission seminar before the Evaluation committee for
assessing the quality and quantum of work. This would be the qualifying exercise for the
students for getting approval from the Department Committee for the submission of Thesis.
Distribution of marks
Total Marks: 100
Project evaluation by the supervisor/s: 30 Marks

Evaluation by the External expert: 30 Marks
Presentation & evaluation by the Committee: 40 Marks
90

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SCHEME AND SYLLABUS FOR M.

TECH (FULL TIME) DEGREE COURSE

BIOTECHNOLOGY AND BIOCHEMICAL ENGINEERING (2015 Scheme)

APJ ABDUL KALAM TECHNOLOGICAL UNIVERSITY

M.Tech (Full Time) Degree Course in

SEMESTER I (Credits: 23)

End Semester Exam

S 03 RM 6001 Research Methodology 1-1-0 100 2

TOTAL 19-1-4 500 300 23

End Semester Exam

Bioreactor Design and

C 03 BH 6022 Bioreaction Engineering 3-0-0 40 60 3 3

E Elective III 3-0-0 40 60 3 3

V 03 BH 6902 Mini Project 0-0-4 100 2

TOTAL 16-0-6 400 300 19

03 BH 6032: Applied Bioinformatics

End Semester Exam

T 03 BH 7903 Seminar II 0-0-2 100 2

03 BH 7913 Project (Phase I) 0-0-8 50 6

TOTAL 6-0-10 230 120 6 14

03 BH 7003: Advanced Fermentation Technology

03 BH 7033: Food Biotechnology

End Semester Exam

03 BH 6001 MATHEMATICAL 4-0-0-4 2015

2. Demin, O. (2009). Kinetic modelling in systems biology. Champman and Hall/CRC.

3. Jones, D. S. (2010). Differential equations and mathematical biology. CRC Press.

Module Content Hours Semester

I Modelling as a mathematical tools to investigate 16 25%

First Internal Exam

II Differential equations - homogeneous and 16 25%

III Method of least squares, approximation theory, linear 15 25%

Second Internal Exam

End semester Exam

03 BH 6011 ADVANCED BIOANALYTICAL 4-0-0-4 2015

1. Freifelder D., Physical Biochemistry, Application to Biochemistry and Molecular

2. Spectrometric identification of organic compounds. Silverstein, Webster and

4. Modern Spectroscopy, J.M. Hollas, John Wiley and Son Ltd.

5. Principles and Techniques of Biochemistry and Molecular Biology, Wilson K. and

Module Content Hours Semester Exam

I Electrophoretic techniques: Basic principles of 16 25%

First Internal Exam

II Chromatography Techniques: TLC and Paper 16 25%

Radioisotope Techniques: Basic physical principles,

Second Internal Exam

IV Biosensors: Principles and applications of 15 25%

End semester Exam

03 BH 6021 ADVANCED DOWNSTREAM 4-0-0-4 2015

1. Asenjo.J.M, Separation Process in Biotechnology, Taylor & Francis, 1990

Module Content Hours Semester

I Role of Downstream Processing In Biotechnology: Role 17 25%

Physio-chemical basis of Bio-separation process

II Enrichment Operations (Membrane based 16 25%

III Product Resolution/Fractionation: Adsorptive 15 25%

Second Internal Exam

End semester Exam

03 BH 6031 GENOMICS AND 3-0-0-3 2015

Module Content Hours Semester

I Genomes of Bacteria, archae and eukaryote. Physical 15 25%

First Internal Exam

II Gene finding; annotation; ORF and functional 14 25%

III Protein level estimation; Edman protein 15 25%

Second Internal Exam

IV Post translational modification; protein-protein 15 25%

End semester Exam